##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	947215
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10478085756666	33.0	31.0	34.0	30.0	34.0
2	32.26356529404623	34.0	31.0	34.0	30.0	34.0
3	32.261591085445225	34.0	31.0	34.0	30.0	34.0
4	35.84895087176618	37.0	35.0	37.0	35.0	37.0
5	35.873472231753084	37.0	35.0	37.0	35.0	37.0
6	35.72840168282808	37.0	35.0	37.0	35.0	37.0
7	36.05389167190131	37.0	35.0	37.0	35.0	37.0
8	36.06913953009612	37.0	35.0	37.0	35.0	37.0
9	37.71441752928322	39.0	38.0	39.0	35.0	39.0
10	37.3147859778403	39.0	37.0	39.0	34.0	39.0
11	37.177945872901084	39.0	37.0	39.0	34.0	39.0
12	37.01125087757267	39.0	37.0	39.0	33.0	39.0
13	36.949387414684104	39.0	37.0	39.0	33.0	39.0
14	38.10262295255037	40.0	37.0	41.0	33.0	41.0
15	38.122083159578345	40.0	37.0	41.0	33.0	41.0
16	38.18701773092698	40.0	37.0	41.0	34.0	41.0
17	38.14538726688239	40.0	37.0	41.0	33.0	41.0
18	38.09405467607671	40.0	37.0	41.0	33.0	41.0
19	38.01480339732796	40.0	37.0	41.0	34.0	41.0
20	37.88494270044288	40.0	36.0	41.0	33.0	41.0
21	37.77206230897948	40.0	36.0	41.0	33.0	41.0
22	37.755865352639056	40.0	36.0	41.0	33.0	41.0
23	37.744661982759986	39.0	36.0	41.0	33.0	41.0
24	37.79172732695323	40.0	36.0	41.0	33.0	41.0
25	37.71030758592294	40.0	36.0	41.0	33.0	41.0
26	37.63839571797322	40.0	36.0	41.0	33.0	41.0
27	37.555395554335604	39.0	36.0	41.0	33.0	41.0
28	37.48006735535227	39.0	36.0	41.0	33.0	41.0
29	37.430669911266186	39.0	35.0	41.0	33.0	41.0
30	37.35617573623728	39.0	35.0	41.0	32.0	41.0
31	37.22089388364838	39.0	35.0	41.0	32.0	41.0
32	37.12557972582782	39.0	35.0	41.0	32.0	41.0
33	36.972790760281455	39.0	35.0	41.0	31.0	41.0
34	36.84741795685246	39.0	35.0	41.0	31.0	41.0
35	36.79122585685404	39.0	35.0	41.0	31.0	41.0
36	36.685696489181446	39.0	35.0	41.0	31.0	41.0
37	36.57500989743617	39.0	35.0	41.0	31.0	41.0
38	36.46558278743474	39.0	35.0	40.0	30.0	41.0
39	36.39057236213531	39.0	35.0	40.0	30.0	41.0
40	36.256203712990185	38.0	35.0	40.0	30.0	41.0
41	36.2416821946443	38.0	35.0	40.0	30.0	41.0
42	36.15141335388481	38.0	35.0	40.0	30.0	41.0
43	36.06764250988424	38.0	35.0	40.0	30.0	41.0
44	35.95354697719103	38.0	35.0	40.0	30.0	41.0
45	35.849979149401136	38.0	35.0	40.0	29.0	41.0
46	35.83488437155239	38.0	35.0	40.0	29.0	41.0
47	35.764979439725934	38.0	35.0	40.0	29.0	41.0
48	35.68100484050611	38.0	35.0	40.0	29.0	41.0
49	35.59195008524992	38.0	34.0	40.0	29.0	41.0
50	35.43013571364474	37.0	34.0	40.0	28.0	41.0
51	33.94070511974578	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	19.0
10	25.0
11	17.0
12	16.0
13	16.0
14	16.0
15	34.0
16	47.0
17	116.0
18	234.0
19	456.0
20	844.0
21	1305.0
22	1876.0
23	2891.0
24	4115.0
25	5592.0
26	7530.0
27	9018.0
28	10912.0
29	12952.0
30	16382.0
31	20667.0
32	27222.0
33	37920.0
34	65331.0
35	80228.0
36	79674.0
37	114611.0
38	188790.0
39	258297.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.88088237622926	20.706492190263035	25.89380446889038	11.518820964617325
2	31.305458633995446	24.40280189819629	26.77343580918799	17.518303658620273
3	31.190595588118853	24.67971896559915	27.5011481025955	16.628537343686492
4	28.291359406259403	25.769334311639913	27.443505434352282	18.4958008477484
5	25.822648501132267	30.136347080652225	25.286444999287383	18.75455941892812
6	25.887364537090313	34.62888573343961	24.474169011259324	15.009580718210755
7	81.13648960373305	5.858965493578543	8.549484541524363	4.455060361164044
8	82.0400859361391	5.178549748473156	7.941280490701688	4.840083824686054
9	76.35711005421155	7.24017250571412	10.202541133744715	6.200176306329609
10	42.070385287395155	24.238108560358523	18.825609814033772	14.86589633821255
11	31.69956134562903	25.2197230829326	23.059917758903733	20.020797812534642
12	27.444455588224425	23.050205074877404	28.761052137054417	20.744287199843754
13	24.869327449417504	24.96413169132668	30.913678520715994	19.252862338539824
14	21.86050685430446	27.651061269088856	29.115670676667914	21.37276119993877
15	20.80815865458212	25.50065191112894	33.90940810692398	19.781781327364957
16	22.994462714378468	24.576363338840707	30.599177589037335	21.82999635774349
17	23.45190901748811	25.579514682516642	27.34796218387589	23.62061411611936
18	23.854774259275878	25.660383334301084	29.182920456285004	21.301921950138038
19	25.085856959613185	25.88567537465095	26.997144259750954	22.031323405984914
20	25.81557513341744	26.52998527261498	27.139139477309797	20.51530011665778
21	24.755836848022888	27.017308636370835	27.660351662505345	20.566502853100932
22	22.890051361095423	25.937933837618704	26.693939601885532	24.478075199400347
23	23.307380056270226	26.065254456485594	27.904330062340648	22.723035424903536
24	23.70676140052681	24.903216270857197	28.021410133918913	23.368612194697086
25	23.039753382283852	27.12815992145395	26.438770500889447	23.39331619537275
26	21.972941729174476	27.573254224225757	27.738475425325827	22.715328621273944
27	24.096957924019364	27.159409426582137	27.038317594210397	21.705315055188105
28	21.248396615340763	26.763300834551817	29.063728931657547	22.924573618449877
29	23.898375764741903	25.695539027570298	28.35565315160761	22.050432056080194
30	24.59895588646717	25.574975058460858	28.39703763137197	21.429031423700003
31	24.626510348759258	27.09933858733234	27.054681355341714	21.21946970856669
32	26.508237306208198	26.503697682152417	26.522595186942777	20.465469824696612
33	26.008034078852216	26.463368928912658	26.076339584993903	21.45225740724123
34	23.37072364774629	26.37236530249204	28.95636154410561	21.300549505656054
35	25.117951045961057	25.160813542859856	27.74586551099803	21.975369900181057
36	25.376604044488317	27.57378208748806	27.24946289913061	19.800150968893018
37	25.225846296775284	27.518250872294043	27.453534836335997	19.80236799459468
38	24.28371594622129	27.612104960331074	26.72466124375142	21.379517849696214
39	25.702612395285122	25.521977586925882	27.019208944115114	21.756201073673875
40	24.348009691569498	25.10464889175108	29.056972281900094	21.49036913477933
41	23.041548117375672	26.67335293465581	26.649387942547364	23.635711005421157
42	24.587026176739176	25.089868720406667	27.661407389029947	22.66169771382421
43	25.728266549832927	24.063069102579668	27.295492575603213	22.913171771984185
44	23.0449264422544	25.09134673754111	27.530075009369575	24.333651810834922
45	23.29481691062747	24.27220852710314	27.758850947250625	24.674123615018765
46	24.13464736094762	25.81040207344689	27.801291153539587	22.2536594120659
47	23.40936323854669	25.661544633478144	29.420775642277626	21.508316485697545
48	23.25079311455161	24.285932971922954	29.134568181458278	23.328705732067164
49	23.634866424201476	23.441035034284717	30.53625628817111	22.387842253342694
50	23.167496291760585	23.77570034258326	29.362605110772105	23.694198254884054
51	22.796830708973147	23.725764477969626	27.267726968006205	26.209677845051015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	332.0
1	908.5
2	1485.0
3	2750.0
4	4015.0
5	3004.5
6	1994.0
7	1857.0
8	1720.0
9	1725.0
10	1730.0
11	1874.5
12	2019.0
13	2109.5
14	2200.0
15	2207.5
16	2215.0
17	2494.5
18	2774.0
19	2467.5
20	2161.0
21	2382.0
22	2603.0
23	3281.5
24	3960.0
25	5266.5
26	7363.0
27	8153.0
28	9576.0
29	10999.0
30	13728.5
31	16458.0
32	17561.0
33	18664.0
34	20412.0
35	22160.0
36	25652.0
37	29144.0
38	30245.0
39	31346.0
40	37362.5
41	43379.0
42	46490.0
43	49601.0
44	55669.5
45	61738.0
46	74233.5
47	86729.0
48	89388.5
49	92048.0
50	89820.0
51	87592.0
52	77364.0
53	67136.0
54	58591.0
55	50046.0
56	45864.5
57	41683.0
58	41797.5
59	41912.0
60	39850.5
61	37789.0
62	35263.0
63	32737.0
64	29027.5
65	25318.0
66	21037.5
67	16757.0
68	14298.5
69	11840.0
70	10481.5
71	9123.0
72	7548.0
73	5973.0
74	5623.0
75	4368.0
76	3463.0
77	2660.0
78	1857.0
79	1614.5
80	1372.0
81	946.0
82	520.0
83	380.0
84	240.0
85	265.0
86	290.0
87	170.0
88	50.0
89	33.5
90	17.0
91	12.0
92	7.0
93	7.5
94	8.0
95	10.0
96	12.0
97	6.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	947215.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.130486550611913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.9414846016435	18.07924511212656
2	11.602698899379336	5.831629372833041
3	4.490786871339512	3.3856697721558646
4	2.178147083341717	2.189515839326944
5	1.302487818093176	1.636607629746321
6	0.8294693164772833	1.2506980501126574
7	0.6246856507361075	1.09890580409288
8	0.47297360843902636	0.9508845524537072
9	0.3775713816233381	0.8539697275003131
>10	4.408673535324086	26.118576077904642
>50	1.4569652277883987	26.159614245429186
>100	0.3072656056888059	10.202607908202445
>500	0.0033952000628597336	0.5969412452536466
>1k	0.0033952000628597336	1.6451346628618007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	3301	0.3484953257708123	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	2885	0.3045771023474079	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2134	0.2252920403498678	No Hit
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	1958	0.206711253516889	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	1494	0.15772554277539946	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	1381	0.1457958330474074	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	1154	0.12183084093896318	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1118	0.11803022545039932	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0557265246010673E-4	0.0	0.0	0.22560875830724808	0.0
2	1.0557265246010673E-4	0.0	0.0	1.1668945276415597	0.0
3	1.0557265246010673E-4	0.0	0.0	1.5998479753804575	0.0
4	1.0557265246010673E-4	0.0	0.0	2.084109732214967	0.0
5	1.0557265246010673E-4	0.0	0.0	3.5025838906689613	0.0
6	1.0557265246010673E-4	0.0	0.0	4.231985346515838	0.0
7	1.0557265246010673E-4	0.0	0.0	5.008894495969764	0.0
8	1.0557265246010673E-4	0.0	0.0	6.205982802214915	0.0
9	1.0557265246010673E-4	0.0	0.0	6.620883326383134	0.0
10	1.0557265246010673E-4	0.0	0.0	7.707014774892712	0.0
11	1.0557265246010673E-4	0.0	0.0	9.051693649277091	0.0
12	1.0557265246010673E-4	0.0	0.0	10.144687320196576	0.0
13	1.0557265246010673E-4	0.0	0.0	10.530660937590726	0.0
14	1.0557265246010673E-4	0.0	0.0	10.68310784774312	0.0
15	1.0557265246010673E-4	0.0	0.0	11.00943291649731	0.0
16	1.0557265246010673E-4	0.0	0.0	11.697555465232286	0.0
17	1.0557265246010673E-4	0.0	0.0	12.528728958050706	0.0
18	1.0557265246010673E-4	0.0	0.0	13.464524949457093	0.0
19	1.0557265246010673E-4	0.0	0.0	13.991965921147786	0.0
20	1.0557265246010673E-4	0.0	0.0	14.495653046034954	0.0
21	1.0557265246010673E-4	0.0	0.0	15.141757679090809	0.0
22	1.0557265246010673E-4	0.0	0.0	15.798525150045132	0.0
23	1.0557265246010673E-4	0.0	0.0	16.495726946891676	0.0
24	1.0557265246010673E-4	0.0	0.0	17.0312970128218	0.0
25	1.0557265246010673E-4	0.0	0.0	17.532028103440084	0.0
26	1.0557265246010673E-4	0.0	0.0	18.006471603595806	0.0
27	1.0557265246010673E-4	0.0	0.0	18.46719065893171	0.0
28	1.0557265246010673E-4	0.0	0.0	18.92347566286429	0.0
29	1.0557265246010673E-4	0.0	0.0	19.41766124903005	0.0
30	1.0557265246010673E-4	0.0	0.0	19.983847384173604	0.0
31	1.0557265246010673E-4	0.0	0.0	20.509493620772474	0.0
32	1.0557265246010673E-4	0.0	0.0	21.004523788157915	0.0
33	1.0557265246010673E-4	0.0	0.0	21.49501433148757	0.0
34	1.0557265246010673E-4	0.0	0.0	21.981915404633583	0.0
35	1.0557265246010673E-4	0.0	0.0	22.456042186831922	0.0
36	2.1114530492021347E-4	0.0	0.0	22.953817243181327	0.0
37	2.1114530492021347E-4	0.0	0.0	23.43290594004529	0.0
38	2.1114530492021347E-4	0.0	0.0	23.932264586181596	0.0
39	2.1114530492021347E-4	0.0	0.0	24.435529420458924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	20	7.0326583E-4	45.0	11
TTTCGCG	20	7.0326583E-4	45.0	1
TAGCGTA	20	7.0326583E-4	45.0	1
TAGAACG	50	2.1827873E-11	45.0	1
CACGCTA	25	3.890285E-5	45.0	19
GCACGAA	20	7.0326583E-4	45.0	9
ATTCGGT	20	7.0326583E-4	45.0	39
AAGCGTA	20	7.0326583E-4	45.0	24
TCTAGCG	40	6.8121153E-9	45.0	1
GCGCAAT	20	7.0326583E-4	45.0	19
GCCGATA	20	7.0326583E-4	45.0	9
CTAACGG	40	6.8121153E-9	45.0	2
ATTCACG	25	3.890285E-5	45.0	18
CTAGTCG	20	7.0326583E-4	45.0	23
GTATGCG	25	3.890285E-5	45.0	1
TCGCTAA	25	3.890285E-5	45.0	20
ACTCGGT	25	3.890285E-5	45.0	20
TCGTAAG	45	3.8380676E-10	45.0	1
ACATGCG	40	6.8121153E-9	45.0	1
CATCGTT	25	3.890285E-5	45.0	36
>>END_MODULE