SRR3550231_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003052
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	4034	0.4021725693184401	Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	2666	0.26578881254411535	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT	2368	0.23607948541052706	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1939	0.1933100178255963	No Hit
TCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1695	0.16898426003836292	Illumina PCR Primer Index 5 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1287	0.12830840275479236	No Hit
ACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1190	0.11863791707708074	Illumina PCR Primer Index 5 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCCG	20	7.0328556E-4	45.000004	37
ACGTTTA	40	6.8139343E-9	45.000004	25
CGTGCTA	20	7.0328556E-4	45.000004	32
TCCTCGA	20	7.0328556E-4	45.000004	13
ACGATAA	20	7.0328556E-4	45.000004	41
CCCCGCA	20	7.0328556E-4	45.000004	43
TATCTCG	20	7.0328556E-4	45.000004	1
CTACGTC	20	7.0328556E-4	45.000004	42
TGACATA	20	7.0328556E-4	45.000004	20
TCGTAGT	20	7.0328556E-4	45.000004	26
TGCTCGA	20	7.0328556E-4	45.000004	41
CACAATC	20	7.0328556E-4	45.000004	42
CACGTAA	20	7.0328556E-4	45.000004	15
GACGAAC	20	7.0328556E-4	45.000004	32
TGTCGGT	20	7.0328556E-4	45.000004	23
TCGGTAA	20	7.0328556E-4	45.000004	25
TGCGTAC	20	7.0328556E-4	45.000004	12
CTTACGT	20	7.0328556E-4	45.000004	9
CGGACTA	20	7.0328556E-4	45.000004	23
CGTGGTT	25	3.8904494E-5	45.0	45