##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550228_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1528011
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.910833102641277	33.0	31.0	34.0	30.0	34.0
2	32.07090786650096	33.0	31.0	34.0	30.0	34.0
3	32.0806905185892	33.0	31.0	34.0	30.0	34.0
4	35.6736463284623	37.0	35.0	37.0	33.0	37.0
5	35.72642212654228	37.0	35.0	37.0	35.0	37.0
6	35.59202911497365	37.0	35.0	37.0	33.0	37.0
7	35.971676905467305	37.0	35.0	37.0	35.0	37.0
8	35.97865329503518	37.0	35.0	37.0	35.0	37.0
9	37.57480934365001	39.0	37.0	39.0	35.0	39.0
10	37.189833711930085	39.0	37.0	39.0	34.0	39.0
11	37.08156551229016	39.0	37.0	39.0	33.0	39.0
12	36.93356854106417	39.0	37.0	39.0	33.0	39.0
13	36.84614115997856	39.0	37.0	39.0	33.0	39.0
14	37.926228279770235	40.0	37.0	41.0	33.0	41.0
15	37.981807722588385	40.0	37.0	41.0	33.0	41.0
16	38.054670417948564	40.0	37.0	41.0	33.0	41.0
17	38.03709659158213	40.0	37.0	41.0	33.0	41.0
18	38.005896554409624	40.0	37.0	41.0	33.0	41.0
19	37.90709818188482	40.0	37.0	41.0	33.0	41.0
20	37.768777188122336	40.0	36.0	41.0	33.0	41.0
21	37.64410727409685	39.0	36.0	41.0	33.0	41.0
22	37.642142628554375	39.0	36.0	41.0	33.0	41.0
23	37.64197378160236	39.0	36.0	41.0	33.0	41.0
24	37.683246390241955	39.0	36.0	41.0	33.0	41.0
25	37.596486543617814	39.0	36.0	41.0	33.0	41.0
26	37.53652689673046	39.0	36.0	41.0	33.0	41.0
27	37.451923448195075	39.0	36.0	41.0	32.0	41.0
28	37.381963873296726	39.0	36.0	41.0	32.0	41.0
29	37.342879076132306	39.0	36.0	41.0	32.0	41.0
30	37.258663059362796	39.0	35.0	41.0	32.0	41.0
31	37.137217598564405	39.0	35.0	41.0	32.0	41.0
32	37.030433681432925	39.0	35.0	41.0	31.0	41.0
33	36.871312444740255	39.0	35.0	41.0	31.0	41.0
34	36.777535632924106	39.0	35.0	41.0	31.0	41.0
35	36.70007872979972	39.0	35.0	41.0	31.0	41.0
36	36.58083089715977	39.0	35.0	40.0	30.0	41.0
37	36.47989379657607	39.0	35.0	40.0	30.0	41.0
38	36.38959798064281	39.0	35.0	40.0	30.0	41.0
39	36.28594493102471	39.0	35.0	40.0	30.0	41.0
40	36.10575709206282	38.0	35.0	40.0	30.0	41.0
41	36.05712851543608	38.0	35.0	40.0	30.0	41.0
42	35.95467506451197	38.0	35.0	40.0	29.0	41.0
43	35.83883885652656	38.0	35.0	40.0	29.0	41.0
44	35.759943482082264	38.0	35.0	40.0	28.0	41.0
45	35.587444069447145	38.0	34.0	40.0	28.0	41.0
46	35.547943044912635	38.0	34.0	40.0	28.0	41.0
47	35.449097552308196	38.0	34.0	40.0	27.0	41.0
48	35.375870330776415	38.0	34.0	40.0	27.0	41.0
49	35.26032993217981	38.0	34.0	40.0	27.0	41.0
50	35.09883240369343	37.0	34.0	40.0	26.0	41.0
51	33.601266613918355	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	28.0
10	30.0
11	27.0
12	23.0
13	23.0
14	19.0
15	43.0
16	76.0
17	204.0
18	410.0
19	726.0
20	1341.0
21	2002.0
22	3189.0
23	4978.0
24	7301.0
25	10212.0
26	13649.0
27	16879.0
28	20099.0
29	24476.0
30	30244.0
31	37652.0
32	48280.0
33	63515.0
34	101160.0
35	128270.0
36	128659.0
37	184691.0
38	302365.0
39	397311.0
40	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18608373892596	21.62445165643441	25.561596087986278	10.627868516653349
2	31.586683603717514	24.762583515432805	25.815586406118808	17.835146474730877
3	31.815739546377614	24.212980142158663	26.78311870791506	17.188161603548664
4	27.504775816404464	27.218717666299526	26.219248421640945	19.057258095655072
5	24.950147610193905	30.030542973839847	25.07442682022577	19.944882595740477
6	26.82127288350673	34.605968150752844	23.900875059145516	14.671883906594912
7	80.96649827782653	6.3790116694186105	7.886134327567014	4.768355725187842
8	81.08920681853729	5.626399286392571	8.017874216874093	5.266519678196034
9	74.460262393399	8.396470967813713	10.67904615869912	6.4642204800881675
10	38.93486368880853	28.887553819965955	17.665448743497265	14.512133747728257
11	30.801414387723646	26.32965338600311	23.399307989274945	19.469624236998293
12	28.510396849237342	23.798388885943883	28.322309198035878	19.368905066782897
13	25.574488665330286	25.412840614367305	29.230941400290966	19.78172932001144
14	21.014770181628272	29.88892095672086	27.54181743456035	21.55449142709051
15	19.705682747048286	26.782398817809554	33.61683914579149	19.895079289350665
16	21.953637768314497	25.933452049756184	31.076281518915767	21.036628663013552
17	22.02477599964922	25.990977813641393	27.182657716469315	24.80158847024007
18	22.671957204496564	26.835474351951653	28.806271682599142	21.686296760952636
19	24.9033547533362	28.199535212770066	25.989014476990025	20.908095556903714
20	26.138031728829176	27.36832391913409	25.892745536517737	20.600898815519
21	23.76324515988432	27.90555827150459	27.238940033808657	21.092256534802434
22	22.52045305956567	27.59757619545933	25.24026332271168	24.64170742226332
23	22.975816273573948	27.364462690386393	26.536785402722884	23.12293563331678
24	23.319989188559507	25.423246298619578	27.676305995179355	23.58045851764156
25	22.553764338083955	27.35883445865246	26.077495515411865	24.009905687851724
26	21.741270187191063	29.092657055479314	26.270164285466528	22.895908471863095
27	23.442370506495045	28.69763372122321	26.062443267751345	21.797552504530397
28	19.908429978579996	28.727476438324068	28.34600012696244	23.018093456133496
29	23.142438110720406	26.755697439350897	26.999674740561424	23.102189709367277
30	23.58798464147182	28.15679991832519	25.70963167149975	22.54558376870324
31	22.835372258445783	29.042788304534454	24.94949316464345	23.172346272376313
32	24.27325457735579	27.733766314509516	25.48849452000018	22.50448458813451
33	23.45539397294915	29.368702188662255	24.788761337451103	22.387142500937493
34	22.002852073708894	26.71440192511703	27.478859772606352	23.803886228567723
35	22.165678126662698	26.32225815128294	26.12167059006774	25.390393131986617
36	22.88753156881724	30.573798225274558	24.9963514660562	21.542318739852004
37	22.003964631144672	28.851821092910978	27.344763879317625	21.799450396626728
38	21.070070830641928	28.116682406082155	27.192408955171132	23.620837808104785
39	23.86946167272356	27.0999357988915	26.18024346683368	22.85035906155126
40	22.981968061748244	25.323312463064727	28.19456142658659	23.500158048600436
41	20.580414669789683	26.97696547996055	25.483913401146978	26.958706449102788
42	23.53850855785724	26.23816189804916	26.257991598228024	23.96533794586557
43	23.11815818079844	26.18809681343917	27.552157674257582	23.141587331504812
44	22.425493010194298	25.68666063267869	27.927416752889865	23.96042960423714
45	21.46352349557693	25.41349505991776	26.77474180486921	26.348239639636102
46	22.056778387066586	27.623099571927167	26.189405704540086	24.130716336466165
47	20.880805177449638	26.092089651187067	28.849726867149517	24.17737830421378
48	20.948016735481616	24.546289261006628	28.92662421932826	25.57906978418349
49	22.170782801956268	24.45807000080497	29.51346554442344	23.857681652815327
50	20.145208378735493	24.461538562222394	28.81327424998904	26.579978809053078
51	20.33643736857915	24.297469062722715	27.063483181731023	28.30261038696711
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	388.0
1	1197.5
2	2007.0
3	4010.5
4	6014.0
5	4399.5
6	2785.0
7	2619.0
8	2453.0
9	2582.0
10	2711.0
11	2757.0
12	2803.0
13	2893.0
14	2983.0
15	3122.5
16	3262.0
17	3377.5
18	3493.0
19	3405.5
20	3318.0
21	4063.5
22	4809.0
23	5553.5
24	6298.0
25	7233.5
26	10513.5
27	12858.0
28	15261.0
29	17664.0
30	21627.5
31	25591.0
32	28644.5
33	31698.0
34	35223.0
35	38748.0
36	41571.0
37	44394.0
38	49441.5
39	54489.0
40	62516.0
41	70543.0
42	77179.5
43	83816.0
44	94198.0
45	104580.0
46	119848.0
47	135116.0
48	150261.0
49	165406.0
50	158392.0
51	151378.0
52	128699.5
53	106021.0
54	95622.5
55	85224.0
56	77793.5
57	70363.0
58	68057.5
59	65752.0
60	63227.0
61	60702.0
62	54159.5
63	47617.0
64	40765.0
65	33913.0
66	29130.5
67	24348.0
68	20874.5
69	17401.0
70	13960.5
71	10520.0
72	9006.5
73	7493.0
74	6278.0
75	3928.5
76	2794.0
77	2194.0
78	1594.0
79	1229.0
80	864.0
81	595.5
82	327.0
83	231.0
84	135.0
85	92.0
86	49.0
87	37.0
88	25.0
89	18.5
90	12.0
91	9.5
92	7.0
93	8.0
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1528011.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.75996187656459
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73827068119536	17.267169234039393
2	12.382518338151598	6.13181363976991
3	5.182559771452954	3.8495994709257735
4	2.7811395694375527	2.754436388507162
5	1.5701611288560136	1.943856484526926
6	1.0589777697133727	1.5732149523739505
7	0.7401735583568261	1.282866836086932
8	0.5405985639092397	1.0708159866334672
9	0.4115912749949244	0.9171885849840848
>10	3.742510424386479	21.033962112168787
>50	1.3805367019750372	24.556421773497743
>100	0.46373722902862	15.375500919528617
>500	0.004549066859836327	0.8093136055982004
>1k	0.002675921682256663	1.4338400113590386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	4387	0.28710526298567224	No Hit
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	3381	0.2212680406096553	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3275	0.21433091777480656	No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCT	2926	0.19149076806384246	No Hit
TCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	2055	0.13448856061900077	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2722277522871234E-4	0.0	0.0	0.22323137726102757	0.0
2	3.2722277522871234E-4	0.0	0.0	1.074141481965771	0.0
3	3.2722277522871234E-4	0.0	0.0	1.5601981922905006	0.0
4	3.2722277522871234E-4	0.0	0.0	2.1342123845967076	0.0
5	3.2722277522871234E-4	0.0	0.0	3.675693434144126	0.0
6	3.2722277522871234E-4	0.0	0.0	4.572938283821255	0.0
7	3.2722277522871234E-4	0.0	0.0	5.496491844626773	0.0
8	3.2722277522871234E-4	0.0	0.0	7.026781875261369	0.0
9	3.2722277522871234E-4	0.0	0.0	7.596345837824466	0.0
10	3.2722277522871234E-4	0.0	0.0	8.818261125083524	0.0
11	3.2722277522871234E-4	0.0	0.0	10.527149346437952	0.0
12	3.2722277522871234E-4	0.0	0.0	11.810778849105144	0.0
13	3.2722277522871234E-4	0.0	0.0	12.324911273544497	0.0
14	3.2722277522871234E-4	0.0	0.0	12.517318265378979	0.0
15	3.2722277522871234E-4	0.0	0.0	12.896765795534195	0.0
16	3.2722277522871234E-4	0.0	0.0	13.742898447720599	0.0
17	3.2722277522871234E-4	0.0	0.0	14.793479889869904	0.0
18	3.2722277522871234E-4	0.0	0.0	15.942424498252958	0.0
19	3.2722277522871234E-4	0.0	0.0	16.577433015861796	0.0
20	3.9266733027445484E-4	0.0	0.0	17.196800284814703	0.0
21	4.581118853201973E-4	0.0	0.0	17.971467482891157	0.0
22	4.581118853201973E-4	0.0	0.0	18.810990235017943	0.0
23	4.581118853201973E-4	0.0	0.0	19.609937363016364	0.0
24	4.581118853201973E-4	0.0	0.0	20.27681737893248	0.0
25	4.581118853201973E-4	0.0	0.0	20.86837071199095	0.0
26	4.581118853201973E-4	0.0	0.0	21.403510838599985	0.0
27	4.581118853201973E-4	0.0	0.0	21.938716409764066	0.0
28	4.581118853201973E-4	0.0	0.0	22.480400991877676	0.0
29	4.581118853201973E-4	0.0	0.0	23.052255513867372	0.0
30	4.581118853201973E-4	0.0	0.0	23.71821930601285	0.0
31	4.581118853201973E-4	0.0	0.0	24.33797924229603	0.0
32	4.581118853201973E-4	0.0	0.0	24.905776201872893	0.0
33	4.581118853201973E-4	0.0	0.0	25.45505235237181	0.0
34	4.581118853201973E-4	0.0	0.0	26.009236844499156	0.0
35	4.581118853201973E-4	0.0	0.0	26.582465702144816	0.0
36	4.581118853201973E-4	0.0	0.0	27.19803718690507	0.0
37	4.581118853201973E-4	0.0	0.0	27.76570325737184	0.0
38	4.581118853201973E-4	0.0	0.0	28.333565661503744	0.0
39	4.581118853201973E-4	0.0	0.0	28.90973952412646	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	20	7.0340163E-4	45.000004	34
CACGGAT	20	7.0340163E-4	45.000004	10
CGACGAA	20	7.0340163E-4	45.000004	22
ACGTTAG	115	0.0	45.000004	1
ATCACGT	30	2.1659725E-6	45.000004	12
CGGATAG	20	7.0340163E-4	45.000004	12
CATCGAA	35	1.2122837E-7	45.000004	38
ATAACGC	30	2.1659725E-6	45.000004	11
TAAATCG	30	2.1659725E-6	45.000004	25
ATACGGA	20	7.0340163E-4	45.000004	14
TATACGG	115	0.0	45.000004	2
CAATACG	20	7.0340163E-4	45.000004	24
ACGACTA	30	2.1659725E-6	45.000004	12
CCCGCGA	30	2.1659725E-6	45.000004	29
TAATCCG	35	1.2122837E-7	45.000004	1
TCGCAAA	25	3.891412E-5	45.0	15
CGAATCG	25	3.891412E-5	45.0	2
ATACCGG	25	3.891412E-5	45.0	2
GTATACG	75	0.0	42.0	1
TGATTCG	170	0.0	41.02941	15
>>END_MODULE