FastQCFastQC Report
Sat 18 Jun 2016
SRR3550226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797780
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC24540.30760359998997217No Hit
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC19500.24442828850058915No Hit
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT15940.19980445736919955Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC10610.13299405851237184No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10510.13174058010980472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG950.045.0000042
TCGATCA302.1646629E-645.00000417
CTGCGTA302.1646629E-645.0000041
CGGCTAC1200.045.00000425
CTGTCGA453.8380676E-1045.012
CGAACTG207.031989E-445.027
GCGACGT207.031989E-445.010
CGTATGG551.8189894E-1245.02
GTCGACT207.031989E-445.044
GTCGAAA253.8897295E-545.017
CACGAAT253.8897295E-545.012
TCGATAG207.031989E-445.01
CTAACGG351.2113014E-745.02
TACGACA207.031989E-445.026
TCGTCGA207.031989E-445.042
TCCCGTT207.031989E-445.039
CGTCGAC207.031989E-445.043
CGAGACA1000.045.022
ATACGAC207.031989E-445.025
CGGTACA207.031989E-445.019