##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	797780
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5201183283612	34.0	31.0	34.0	31.0	34.0
2	32.68600240667853	34.0	31.0	34.0	31.0	34.0
3	32.70396851262253	34.0	31.0	34.0	31.0	34.0
4	36.1369625711349	37.0	35.0	37.0	35.0	37.0
5	36.12834866755246	37.0	35.0	37.0	35.0	37.0
6	36.149107523377374	37.0	36.0	37.0	35.0	37.0
7	36.29998871869438	37.0	37.0	37.0	35.0	37.0
8	36.223595477449926	37.0	37.0	37.0	35.0	37.0
9	37.79183860212088	39.0	38.0	39.0	35.0	39.0
10	37.669525433076785	39.0	37.0	39.0	35.0	39.0
11	37.63426257865577	39.0	37.0	39.0	35.0	39.0
12	37.39794554889819	39.0	37.0	39.0	35.0	39.0
13	37.206948030785426	39.0	37.0	39.0	34.0	39.0
14	38.3849106269899	40.0	38.0	41.0	34.0	41.0
15	38.49395823409963	40.0	38.0	41.0	35.0	41.0
16	38.55460527965103	40.0	38.0	41.0	35.0	41.0
17	38.57861064453859	40.0	38.0	41.0	35.0	41.0
18	38.54017022236707	40.0	37.0	41.0	35.0	41.0
19	38.53158264183108	40.0	37.0	41.0	35.0	41.0
20	38.45988242372584	40.0	37.0	41.0	35.0	41.0
21	38.40772894783023	40.0	37.0	41.0	34.0	41.0
22	38.39620572087543	40.0	37.0	41.0	35.0	41.0
23	38.364399959888694	40.0	37.0	41.0	34.0	41.0
24	38.260393842914084	40.0	36.0	41.0	34.0	41.0
25	38.23763694251548	40.0	36.0	41.0	34.0	41.0
26	38.20467547444157	40.0	36.0	41.0	34.0	41.0
27	38.15884705056532	40.0	36.0	41.0	34.0	41.0
28	38.09241018827245	40.0	36.0	41.0	34.0	41.0
29	37.98012108601369	40.0	36.0	41.0	34.0	41.0
30	37.87946677028755	40.0	36.0	41.0	34.0	41.0
31	37.8493644864499	40.0	36.0	41.0	34.0	41.0
32	37.73870866654968	40.0	35.0	41.0	34.0	41.0
33	37.65092381358269	40.0	35.0	41.0	33.0	41.0
34	37.57679936824689	40.0	35.0	41.0	33.0	41.0
35	37.41575120960666	40.0	35.0	41.0	33.0	41.0
36	37.350198049587604	40.0	35.0	41.0	33.0	41.0
37	37.271888239865625	39.0	35.0	41.0	33.0	41.0
38	37.177458697886635	39.0	35.0	41.0	33.0	41.0
39	37.11203339266464	39.0	35.0	41.0	33.0	41.0
40	37.075767755521575	39.0	35.0	41.0	32.0	41.0
41	37.04692897791371	39.0	35.0	41.0	33.0	41.0
42	36.99273985309233	39.0	35.0	41.0	33.0	41.0
43	36.91272029882925	39.0	35.0	41.0	32.0	41.0
44	36.70992880242673	39.0	35.0	41.0	32.0	41.0
45	36.677874852716286	39.0	35.0	41.0	31.0	41.0
46	36.63186592795006	39.0	35.0	41.0	31.0	41.0
47	36.583568151620746	39.0	35.0	41.0	31.0	41.0
48	36.530304093860465	39.0	35.0	41.0	31.0	41.0
49	36.46776930983479	38.0	35.0	41.0	31.0	41.0
50	36.33996966582266	38.0	35.0	40.0	31.0	41.0
51	35.42679184737647	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	8.0
10	10.0
11	7.0
12	10.0
13	11.0
14	20.0
15	29.0
16	43.0
17	76.0
18	162.0
19	250.0
20	467.0
21	761.0
22	1180.0
23	1856.0
24	2505.0
25	3255.0
26	4381.0
27	5343.0
28	6020.0
29	7125.0
30	8698.0
31	11484.0
32	15226.0
33	22577.0
34	47490.0
35	80709.0
36	53006.0
37	82023.0
38	154403.0
39	288326.0
40	313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.06603324224724	22.478753541076486	27.07450675624859	11.380706460427687
2	29.95199177718168	24.919777382235704	27.112111108325603	18.016119732257014
3	31.072350773396174	23.892677179172203	27.434881796986637	17.600090250444985
4	27.830855624357593	25.942615758730476	27.558850811000525	18.667677805911403
5	25.146280929579586	30.613201634535837	25.02293865476698	19.2175787811176
6	26.678407581037376	34.49735516057058	23.42036651708491	15.403870741307127
7	83.40394595001128	5.359372258015995	6.988894181353256	4.2477876106194685
8	84.18097721176264	4.256311263756926	6.531499912256512	5.031211612223921
9	77.329589611171	7.219910250946376	9.414124194640126	6.036375943242498
10	40.287547945548894	30.592644588733737	14.866003158765576	14.253804306951793
11	34.64163052470606	23.54157787861315	23.107498307804157	18.709293288876633
12	31.787710897741235	20.70345207952067	27.32006317531149	20.188773847426607
13	25.07320313870992	25.831682920103287	29.62332974002858	19.471784201158215
14	20.37140565068064	28.97365188397804	28.652761412920857	22.002181052420468
15	19.195768256912935	24.48607385494748	36.05806111960691	20.26009676853268
16	21.389230113565144	23.868610393842914	32.93614781017323	21.80601168241871
17	22.22893529544486	23.421745343327736	27.934392940409637	26.414926420817768
18	22.64947729950613	24.08533680964677	30.987740981222895	22.277444909624208
19	24.795181629020533	24.9962395647923	27.14043972022362	23.068139085963548
20	26.02308907217529	25.881571360525456	27.2062473363584	20.88909223094086
21	23.733234726365666	27.114367369450225	27.750633006593294	21.401764897590812
22	22.300759607911953	25.68703151244704	25.723257038281233	26.288951841359776
23	21.305748451954173	26.990022311915567	26.951039133595728	24.753190102534532
24	22.319561783950462	24.625460653312945	29.622703000827293	23.432274561909296
25	20.650179247411568	26.88122038657274	27.89866880593647	24.56993156007922
26	20.547017974880294	27.787986662989795	26.759507633683473	24.905487728446438
27	22.787610619469024	27.186693073278345	27.45080097269924	22.574895334553386
28	20.954147760034093	26.444759206798867	29.166186166612352	23.434906866554687
29	21.142044172578906	23.880894482188072	29.478678332372333	25.498383012860685
30	23.410589385544885	26.026974855223244	27.717290481085012	22.84514527814686
31	25.01604452355286	26.062698989696408	25.06066835468425	23.860588132066486
32	26.87996690817017	24.401213367093685	25.63037428865101	23.088445436085138
33	26.24094361854145	23.780365514302186	26.609591616736445	23.369099250419914
34	23.25189901977989	23.64561658602622	29.17809421143674	23.92439018275715
35	23.824362606232295	23.013738123292136	29.19739777883627	23.964501491639297
36	26.852515731153954	23.885908395798342	28.904710571836844	20.35686530121086
37	24.206046779813985	25.812630048384268	29.689137356163354	20.292185815638398
38	23.618416104690514	26.467948557246356	28.49056130762867	21.423074030434456
39	23.125799092481635	24.622452305146783	31.538770086991402	20.71297851538018
40	24.552508210283534	23.038556995662965	30.29619694652661	22.112737847526887
41	22.136553937175663	23.32347263656647	28.604377146581765	25.935596279676098
42	23.623430018300784	23.44794304194139	28.097094437062847	24.831532502694976
43	23.67281706736193	23.501967961092028	28.95459901225902	23.870615959287022
44	22.53240241670636	24.70192283586954	28.4322745619093	24.333400185514805
45	22.204993857955827	24.552132166762767	27.765424051743587	25.477449923537815
46	24.10977963849683	25.452129659805962	27.85579984456868	22.58229085712853
47	22.37471483366342	23.595226754243022	30.418411090776903	23.611647321316653
48	22.80879440447241	22.168893679961894	30.979091980245183	24.043219935320515
49	23.198751535511043	21.437990423425006	31.59241896262127	23.77083907844268
50	21.437990423425006	23.95108927273183	30.439218832259517	24.171701471583646
51	21.58727970117075	22.5545889844318	27.824964275865526	28.033167038531925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	163.0
1	419.0
2	675.0
3	1391.0
4	2107.0
5	1555.0
6	1003.0
7	995.5
8	988.0
9	1077.0
10	1166.0
11	1295.5
12	1425.0
13	1400.5
14	1376.0
15	1353.0
16	1330.0
17	1356.0
18	1382.0
19	1462.5
20	1543.0
21	1854.5
22	2166.0
23	2153.5
24	2141.0
25	2837.5
26	4961.0
27	6388.0
28	7402.0
29	8416.0
30	9778.0
31	11140.0
32	12641.0
33	14142.0
34	16268.5
35	18395.0
36	20915.5
37	23436.0
38	25875.0
39	28314.0
40	31981.0
41	35648.0
42	40698.0
43	45748.0
44	51680.5
45	57613.0
46	63878.0
47	70143.0
48	76181.0
49	82219.0
50	79971.0
51	77723.0
52	69456.0
53	61189.0
54	52653.0
55	44117.0
56	41446.5
57	38776.0
58	35191.0
59	31606.0
60	31618.0
61	31630.0
62	29426.0
63	27222.0
64	22870.5
65	18519.0
66	15628.5
67	12738.0
68	11208.5
69	9679.0
70	8257.0
71	6835.0
72	6418.0
73	6001.0
74	4852.5
75	3169.0
76	2634.0
77	1924.5
78	1215.0
79	1110.5
80	1006.0
81	658.5
82	311.0
83	270.0
84	229.0
85	128.5
86	28.0
87	18.0
88	8.0
89	7.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	797780.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.520071091055375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.55842023529286	17.546175513329963
2	11.420104327050533	5.600435399330963
3	4.276792092114098	3.1460173842090335
4	2.0602897862233998	2.0207380810549216
5	1.2240342297473112	1.500670316564464
6	0.7879030609787315	1.159166344083518
7	0.6290692157932591	1.0797375324711616
8	0.499641631567351	0.9800998660865874
9	0.38200746516480094	0.8430165182839299
>10	5.336853365259247	32.56842383876739
>50	1.6996219344755508	28.211452423165902
>100	0.12110455985708449	4.05316598036549
>500	0.001559286178417397	0.2573135274772168
>1k	0.002598810297362328	1.0335872748094246
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	2454	0.30760359998997217	No Hit
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	1950	0.24442828850058915	No Hit
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT	1594	0.19980445736919955	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	1061	0.13299405851237184	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1051	0.13174058010980472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21033367595076335	0.0
2	0.0	0.0	0.0	1.0291057685076086	0.0
3	0.0	0.0	0.0	1.4623079044348066	0.0
4	0.0	0.0	0.0	1.9501616987139312	0.0
5	0.0	0.0	0.0	3.267191456291208	0.0
6	0.0	0.0	0.0	4.132718293263807	0.0
7	0.0	0.0	0.0	5.055027701872697	0.0
8	0.0	0.0	0.0	6.758880894482188	0.0
9	0.0	0.0	0.0	7.548948331620246	0.0
10	0.0	0.0	0.0	8.986061320163454	0.0
11	0.0	0.0	0.0	10.549900975206198	0.0
12	0.0	0.0	0.0	11.786833462859436	0.0
13	0.0	0.0	0.0	12.280327909950111	0.0
14	0.0	0.0	0.0	12.455062799267969	0.0
15	0.0	0.0	0.0	12.777833487929003	1.2534784025671237E-4
16	0.0	0.0	0.0	13.409962646343603	1.2534784025671237E-4
17	0.0	0.0	0.0	14.183484168567775	1.2534784025671237E-4
18	0.0	0.0	0.0	15.095389706435357	1.2534784025671237E-4
19	1.2534784025671237E-4	0.0	0.0	15.576600065180877	1.2534784025671237E-4
20	1.2534784025671237E-4	0.0	0.0	16.095665471683922	1.2534784025671237E-4
21	1.2534784025671237E-4	0.0	0.0	16.680663842161998	1.2534784025671237E-4
22	1.2534784025671237E-4	0.0	0.0	17.249617689087216	1.2534784025671237E-4
23	1.2534784025671237E-4	0.0	0.0	17.85003384391687	1.2534784025671237E-4
24	1.2534784025671237E-4	0.0	0.0	18.311564591742084	1.2534784025671237E-4
25	1.2534784025671237E-4	0.0	0.0	18.74138233598235	1.2534784025671237E-4
26	2.5069568051342474E-4	0.0	0.0	19.129709945097645	1.2534784025671237E-4
27	2.5069568051342474E-4	0.0	0.0	19.51277294492216	1.2534784025671237E-4
28	2.5069568051342474E-4	0.0	0.0	19.907618641730803	1.2534784025671237E-4
29	2.5069568051342474E-4	0.0	0.0	20.304595251823812	1.2534784025671237E-4
30	2.5069568051342474E-4	0.0	0.0	20.78580561056933	1.2534784025671237E-4
31	2.5069568051342474E-4	0.0	0.0	21.20333926646444	1.2534784025671237E-4
32	2.5069568051342474E-4	0.0	0.0	21.57198726465943	1.2534784025671237E-4
33	2.5069568051342474E-4	0.0	0.0	21.944019654541354	1.2534784025671237E-4
34	2.5069568051342474E-4	0.0	0.0	22.33071774173331	1.2534784025671237E-4
35	2.5069568051342474E-4	0.0	0.0	22.7749504876031	1.2534784025671237E-4
36	2.5069568051342474E-4	0.0	0.0	23.16578505352353	1.2534784025671237E-4
37	2.5069568051342474E-4	0.0	0.0	23.547218531424704	1.2534784025671237E-4
38	2.5069568051342474E-4	0.0	0.0	23.931660357492042	1.2534784025671237E-4
39	2.5069568051342474E-4	0.0	0.0	24.349695404748175	1.2534784025671237E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	95	0.0	45.000004	2
TCGATCA	30	2.1646629E-6	45.000004	17
CTGCGTA	30	2.1646629E-6	45.000004	1
CGGCTAC	120	0.0	45.000004	25
CTGTCGA	45	3.8380676E-10	45.0	12
CGAACTG	20	7.031989E-4	45.0	27
GCGACGT	20	7.031989E-4	45.0	10
CGTATGG	55	1.8189894E-12	45.0	2
GTCGACT	20	7.031989E-4	45.0	44
GTCGAAA	25	3.8897295E-5	45.0	17
CACGAAT	25	3.8897295E-5	45.0	12
TCGATAG	20	7.031989E-4	45.0	1
CTAACGG	35	1.2113014E-7	45.0	2
TACGACA	20	7.031989E-4	45.0	26
TCGTCGA	20	7.031989E-4	45.0	42
TCCCGTT	20	7.031989E-4	45.0	39
CGTCGAC	20	7.031989E-4	45.0	43
CGAGACA	100	0.0	45.0	22
ATACGAC	20	7.031989E-4	45.0	25
CGGTACA	20	7.031989E-4	45.0	19
>>END_MODULE