FastQCFastQC Report
Sat 18 Jun 2016
SRR3550223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808436
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC41540.5138316453003082No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC26370.3261853752183228No Hit
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT19710.2438040859140365No Hit
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC14320.17713214156717416No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10750.13297280180496662No Hit
ACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC10380.12839606351028404No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTC9440.11676867432919859No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGC8690.10749150211024744No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGT207.0320425E-445.00000436
TCCGTAT207.0320425E-445.00000440
TATAGCG302.1646993E-645.0000041
ATAACGC302.1646993E-645.00000411
CGCACTA207.0320425E-445.00000434
ACGTACG406.8102963E-945.0000041
CGCGTAA207.0320425E-445.00000431
GCGTAAC207.0320425E-445.00000432
GGTCGAA253.889776E-545.08
CGACGGT253.889776E-545.028
TAACGAC351.2113378E-745.019
TAGCCGT253.889776E-545.044
TTGAGCG253.889776E-545.01
CGGTCTA253.889776E-545.031
GCTACGA950.045.010
CGTTTAT253.889776E-545.039
AACAGCG351.2113378E-745.01
AATACGG253.889776E-545.02
AAATGCG253.889776E-545.01
CGTTGAT900.042.525