##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808436
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.446750268419514	33.0	31.0	34.0	31.0	34.0
2	32.60324379419026	34.0	31.0	34.0	31.0	34.0
3	32.59664710626444	34.0	31.0	34.0	31.0	34.0
4	36.10074266856004	37.0	35.0	37.0	35.0	37.0
5	36.120197517181325	37.0	35.0	37.0	35.0	37.0
6	36.133435423459616	37.0	36.0	37.0	35.0	37.0
7	36.247788322143	37.0	37.0	37.0	35.0	37.0
8	36.10145639234275	37.0	37.0	37.0	35.0	37.0
9	37.65142200495772	39.0	38.0	39.0	35.0	39.0
10	37.65603708889758	39.0	37.0	39.0	35.0	39.0
11	37.58034897011019	39.0	37.0	39.0	35.0	39.0
12	37.457073905664764	39.0	37.0	39.0	35.0	39.0
13	37.40185122879238	39.0	37.0	39.0	35.0	39.0
14	38.634777521040625	40.0	38.0	41.0	35.0	41.0
15	38.67650129385628	40.0	38.0	41.0	35.0	41.0
16	38.702440267380474	40.0	38.0	41.0	35.0	41.0
17	38.6571651930394	40.0	38.0	41.0	35.0	41.0
18	38.607444497771006	40.0	38.0	41.0	35.0	41.0
19	38.58416126941403	40.0	38.0	41.0	35.0	41.0
20	38.57076132186098	40.0	38.0	41.0	35.0	41.0
21	38.52898435992459	40.0	38.0	41.0	35.0	41.0
22	38.55882964143111	40.0	38.0	41.0	35.0	41.0
23	38.542489448762794	40.0	38.0	41.0	35.0	41.0
24	38.49392901849992	40.0	38.0	41.0	35.0	41.0
25	38.41240493990866	40.0	37.0	41.0	34.0	41.0
26	38.360842416715734	40.0	37.0	41.0	34.0	41.0
27	38.31723104859259	40.0	37.0	41.0	34.0	41.0
28	38.21035678767398	40.0	37.0	41.0	34.0	41.0
29	38.140688935178545	40.0	37.0	41.0	34.0	41.0
30	38.04337387251434	40.0	37.0	41.0	34.0	41.0
31	37.98277538358015	40.0	37.0	41.0	34.0	41.0
32	37.903877363205005	40.0	37.0	41.0	34.0	41.0
33	37.802419733905964	40.0	36.0	41.0	33.0	41.0
34	37.71831289056895	40.0	36.0	41.0	33.0	41.0
35	37.647395712214696	40.0	36.0	41.0	33.0	41.0
36	37.55321138593531	40.0	36.0	41.0	33.0	41.0
37	37.49280833609587	40.0	36.0	41.0	33.0	41.0
38	37.3985893750402	40.0	35.0	41.0	33.0	41.0
39	37.29149493590092	40.0	35.0	41.0	33.0	41.0
40	37.13239390625851	40.0	35.0	41.0	32.0	41.0
41	37.06115635622362	39.0	35.0	41.0	32.0	41.0
42	37.010358272021534	39.0	35.0	41.0	32.0	41.0
43	36.970488696693366	39.0	35.0	41.0	32.0	41.0
44	36.862628581606955	39.0	35.0	41.0	32.0	41.0
45	36.77998382061165	39.0	35.0	41.0	31.0	41.0
46	36.68528492051319	39.0	35.0	41.0	31.0	41.0
47	36.57285425191357	39.0	35.0	41.0	31.0	41.0
48	36.51083573715174	39.0	35.0	41.0	31.0	41.0
49	36.40207388092564	39.0	35.0	41.0	31.0	41.0
50	36.302646096908106	39.0	35.0	40.0	31.0	41.0
51	34.80965469128045	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	36.0
10	32.0
11	21.0
12	23.0
13	28.0
14	18.0
15	38.0
16	77.0
17	131.0
18	213.0
19	329.0
20	600.0
21	910.0
22	1285.0
23	1936.0
24	2679.0
25	3691.0
26	4696.0
27	5664.0
28	6466.0
29	7432.0
30	9308.0
31	11635.0
32	15484.0
33	22032.0
34	44407.0
35	62102.0
36	52649.0
37	84435.0
38	162946.0
39	306971.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.955904981965176	21.693244734277048	25.013977606143218	10.336872677614554
2	31.432049042843218	24.686307883369864	25.681439223389358	18.20020385039756
3	31.87401352735405	24.40675576050547	26.38922561588054	17.33000509625994
4	28.358212647630733	26.07689415117585	26.614722748615844	18.95017045257757
5	25.316413420481027	30.449905743930255	24.461181837523316	19.772498998065398
6	26.37586648788525	34.19825440727528	23.956503668812374	15.469375436027095
7	80.9899608627028	5.613183974983796	8.41105542058988	4.985799741723525
8	80.97907564729923	4.8754632401328	8.046029617681548	6.0994314948864226
9	75.03166608117402	7.093202182980471	10.59749936915229	7.2776323666932194
10	40.51588499275144	26.994097244556155	17.13011295884894	15.359904803843472
11	30.127926020117858	25.348079501655047	24.118421247940468	20.405573230286628
12	27.94667728799806	22.819988224176065	28.87835277003993	20.354981717785947
13	24.97884804734079	25.455323612506124	30.139924496187703	19.425903843965386
14	21.611853010009447	28.911626894398566	27.570642574056574	21.90587752153541
15	19.524612956375027	26.235966730823467	32.888812472477724	21.350607840323786
16	21.81112666927252	26.31290541242597	29.29953638878031	22.576431529521198
17	21.590948448609414	25.514573819077825	27.22763459321455	25.666843139098212
18	22.434676338015624	25.73574160477762	29.345922249875066	22.483659807331687
19	23.30561726593076	27.380275000123692	25.97261873543484	23.341488998510705
20	24.68061788440891	27.42987694758769	27.344898050062096	20.544607117941307
21	23.809924347752943	27.391778693675196	27.498033239489583	21.300263719082277
22	22.804031487959467	25.70444661049236	25.399784274822	26.091737626726175
23	21.952510773889337	27.46240889816881	26.344324102340817	24.240756225601036
24	22.861055173198622	25.414504054742736	28.041180749001775	23.683260023056864
25	22.33943070323439	27.415404558926127	25.520140122409195	24.72502461543029
26	20.874255970788038	27.433093033956922	27.140800261245168	24.551850734009868
27	22.542662622644215	26.477544295404954	26.46529842807594	24.514494653874888
28	19.980307655769906	26.68226056236981	28.96988753593358	24.3675442459267
29	21.81891949393644	24.71092331365748	28.99784274822002	24.472314444186058
30	22.32087635879649	25.64544379517983	26.41483061120484	25.618849234818835
31	22.21721942120341	27.415280863296537	25.659792488211806	24.707707227288246
32	23.82489151893285	25.82579202311624	25.642351404440177	24.70696505351073
33	22.368251784927935	25.997234165722453	25.25926603961229	26.37524800973732
34	21.066850066053465	25.81169072134343	28.29092222513594	24.83053698746716
35	21.21194503955786	24.4666244452251	26.885863568668395	27.435566946548644
36	21.584268884611767	25.38964123319595	27.970179457619405	25.05591042457288
37	21.66219713125096	26.80904858269548	28.48710349365936	23.041650792394204
38	21.684709735835614	26.281857809399877	25.864013972658316	26.169418482106188
39	22.72696911072738	25.30948646522421	27.21254372640506	24.751000697643352
40	22.016585110014894	25.348079501655047	28.445294370859287	24.19004101747077
41	19.613055331529026	25.939963089224133	27.032937672246167	27.41404390700068
42	20.70380339321851	24.7425893948315	29.35965246475912	25.193954747190872
43	22.72597954569069	24.139696896229264	28.66411193959695	24.470211618483095
44	21.76461711254818	24.140933852525123	27.60465887219273	26.48979016273397
45	21.952015991370992	23.826375866487886	26.85444487875354	27.367163263387578
46	22.564804140340115	24.944955444834225	27.32139588044075	25.16884453438491
47	19.723886615638097	25.370221019350947	29.880782152205	25.025110212805963
48	20.893923575892217	23.67571458965212	28.73832931734856	26.692032517107105
49	21.637829092222514	22.43987155445824	30.526473338644	25.39582601467525
50	20.74858121112865	22.599562612253784	29.62584545962822	27.026010716989347
51	20.406439099693728	22.930448421396374	27.116432222216723	29.54668025669317
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	373.0
1	773.0
2	1173.0
3	1730.0
4	2287.0
5	1740.0
6	1193.0
7	1143.5
8	1094.0
9	1143.0
10	1192.0
11	1249.0
12	1306.0
13	1323.0
14	1340.0
15	1429.0
16	1518.0
17	1472.5
18	1427.0
19	1565.0
20	1703.0
21	1730.5
22	1758.0
23	2263.0
24	2768.0
25	3051.0
26	3885.5
27	4437.0
28	5666.0
29	6895.0
30	9290.0
31	11685.0
32	12907.5
33	14130.0
34	15927.5
35	17725.0
36	19963.5
37	22202.0
38	23795.5
39	25389.0
40	28652.5
41	31916.0
42	35862.5
43	39809.0
44	47626.5
45	55444.0
46	65864.0
47	76284.0
48	86259.0
49	96234.0
50	92412.5
51	88591.0
52	74877.5
53	61164.0
54	54117.5
55	47071.0
56	42425.5
57	37780.0
58	35428.0
59	33076.0
60	31185.5
61	29295.0
62	26731.5
63	24168.0
64	21154.5
65	18141.0
66	15944.5
67	13748.0
68	11689.5
69	9631.0
70	8618.5
71	7606.0
72	6293.5
73	4981.0
74	4387.0
75	2958.5
76	2124.0
77	1636.0
78	1148.0
79	964.0
80	780.0
81	542.5
82	305.0
83	268.5
84	232.0
85	174.5
86	117.0
87	72.0
88	27.0
89	20.5
90	14.0
91	11.0
92	8.0
93	9.0
94	10.0
95	6.0
96	2.0
97	3.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	808436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.649341095403734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.32591071534559	21.444155968349975
2	11.616000239199527	6.888135065126363
3	4.323562352507924	3.8457232481026096
4	2.186961793076793	2.593679046621984
5	1.3313406456831527	1.9736686459017425
6	0.8357163466442287	1.4867063412395642
7	0.6563876893349161	1.3623023744340381
8	0.4964847670799696	1.1776356966260875
9	0.442778909413728	1.1815292623552647
>10	4.67479204698407	32.335735343834365
>50	1.032393567608974	19.713749769211873
>100	0.07137328438164947	3.6894816431603825
>500	0.003778585643734384	0.7755143775731025
>1k	0.0025190570958229224	1.531983217462628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	4154	0.5138316453003082	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	2637	0.3261853752183228	No Hit
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	1971	0.2438040859140365	No Hit
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	1432	0.17713214156717416	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1075	0.13297280180496662	No Hit
ACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	1038	0.12839606351028404	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTC	944	0.11676867432919859	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGC	869	0.10749150211024744	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2369562958601546E-4	0.0	0.0	0.2624821259815248	0.0
2	1.2369562958601546E-4	0.0	0.0	1.4906560321410725	0.0
3	1.2369562958601546E-4	0.0	0.0	2.0968883127421343	0.0
4	1.2369562958601546E-4	0.0	0.0	2.823229049671217	0.0
5	1.2369562958601546E-4	0.0	0.0	4.790979125125551	0.0
6	1.2369562958601546E-4	0.0	0.0	5.808128287211356	0.0
7	1.2369562958601546E-4	0.0	0.0	6.8312148395172905	0.0
8	1.2369562958601546E-4	0.0	0.0	8.4312178082124	0.0
9	1.2369562958601546E-4	0.0	0.0	9.026441177780306	0.0
10	1.2369562958601546E-4	0.0	0.0	10.43013918232241	0.0
11	2.473912591720309E-4	0.0	0.0	12.317487098545834	0.0
12	2.473912591720309E-4	0.0	0.0	13.789959873137763	0.0
13	2.473912591720309E-4	0.0	0.0	14.3451058587198	0.0
14	2.473912591720309E-4	0.0	0.0	14.546729734945005	0.0
15	2.473912591720309E-4	0.0	0.0	14.946019227248662	0.0
16	2.473912591720309E-4	0.0	0.0	15.77465624984538	0.0
17	2.473912591720309E-4	0.0	0.0	16.78265193534182	0.0
18	3.710868887580464E-4	0.0	0.0	17.890346298284587	0.0
19	4.947825183440618E-4	0.0	0.0	18.59491660440653	0.0
20	4.947825183440618E-4	0.0	0.0	19.268439307502387	0.0
21	4.947825183440618E-4	0.0	0.0	20.052545903448138	0.0
22	4.947825183440618E-4	0.0	0.0	20.865349885457846	0.0
23	4.947825183440618E-4	0.0	0.0	21.693368429906634	0.0
24	4.947825183440618E-4	0.0	0.0	22.36924134996462	0.0
25	6.184781479300774E-4	0.0	0.0	22.94120994117036	0.0
26	6.184781479300774E-4	0.0	0.0	23.462710715505	0.0
27	6.184781479300774E-4	0.0	0.0	23.96219366777333	0.0
28	6.184781479300774E-4	0.0	0.0	24.511525958764825	0.0
29	6.184781479300774E-4	0.0	0.0	25.099203894927985	0.0
30	6.184781479300774E-4	0.0	0.0	25.73141225774211	0.0
31	6.184781479300774E-4	0.0	0.0	26.305359979021222	0.0
32	6.184781479300774E-4	0.0	0.0	26.855063356901475	0.0
33	6.184781479300774E-4	0.0	0.0	27.38448065152962	0.0
34	7.421737775160928E-4	0.0	0.0	27.914269033046523	0.0
35	7.421737775160928E-4	0.0	0.0	28.497246535285417	0.0
36	7.421737775160928E-4	0.0	0.0	29.034085567688724	0.0
37	7.421737775160928E-4	0.0	0.0	29.568326991870723	0.0
38	7.421737775160928E-4	0.0	0.0	30.10924798005037	0.0
39	7.421737775160928E-4	0.0	0.0	30.642005056677338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGT	20	7.0320425E-4	45.000004	36
TCCGTAT	20	7.0320425E-4	45.000004	40
TATAGCG	30	2.1646993E-6	45.000004	1
ATAACGC	30	2.1646993E-6	45.000004	11
CGCACTA	20	7.0320425E-4	45.000004	34
ACGTACG	40	6.8102963E-9	45.000004	1
CGCGTAA	20	7.0320425E-4	45.000004	31
GCGTAAC	20	7.0320425E-4	45.000004	32
GGTCGAA	25	3.889776E-5	45.0	8
CGACGGT	25	3.889776E-5	45.0	28
TAACGAC	35	1.2113378E-7	45.0	19
TAGCCGT	25	3.889776E-5	45.0	44
TTGAGCG	25	3.889776E-5	45.0	1
CGGTCTA	25	3.889776E-5	45.0	31
GCTACGA	95	0.0	45.0	10
CGTTTAT	25	3.889776E-5	45.0	39
AACAGCG	35	1.2113378E-7	45.0	1
AATACGG	25	3.889776E-5	45.0	2
AAATGCG	25	3.889776E-5	45.0	1
CGTTGAT	90	0.0	42.5	25
>>END_MODULE