FastQCFastQC Report
Sat 18 Jun 2016
SRR3550221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487306
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC31640.649284022770087No Hit
GCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC31590.6482579734294263No Hit
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT21000.4309407230774913No Hit
TCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC11270.23127152138492035No Hit
ACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC8890.18243157276947136No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA7600.15595949978042545No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG7150.1467250557144792No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.1467250557144792No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5520.11327584720894057No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG5060.10383619327486221No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG4960.10178409459354082No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG4940.10137367485727652No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG253.8874874E-545.015
TATCACG207.0292834E-445.01
TTAGGAT253.8874874E-545.032
GTCGCTA207.0292834E-445.022
AGATCGA207.0292834E-445.017
CGAACGT207.0292834E-445.038
GTATTAG551.8189894E-1245.01
AATCACG207.0292834E-445.02
CAAGTAA207.0292834E-445.026
CGCATGG406.8012014E-945.02
TACGGTA207.0292834E-445.019
TCGATAG253.8874874E-545.01
CCCGTTA253.8874874E-545.043
AATGCGA351.21001E-745.025
CGGATAT253.8874874E-545.09
TACGACG351.21001E-745.01
CGCTAAT207.0292834E-445.029
ACATGCG253.8874874E-545.01
ACGTCTT207.0292834E-445.023
ACGTCGC253.8874874E-545.020