##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550221_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487306
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.450936372628284	33.0	31.0	34.0	31.0	34.0
2	32.61742313864389	34.0	31.0	34.0	31.0	34.0
3	32.61376835089246	34.0	31.0	34.0	31.0	34.0
4	36.12624716297357	37.0	35.0	37.0	35.0	37.0
5	36.14292456895667	37.0	35.0	37.0	35.0	37.0
6	36.14966571312481	37.0	36.0	37.0	35.0	37.0
7	36.2402494531157	37.0	37.0	37.0	35.0	37.0
8	36.09167340439067	37.0	37.0	37.0	35.0	37.0
9	37.62775750760303	39.0	38.0	39.0	35.0	39.0
10	37.67735468063188	39.0	37.0	39.0	35.0	39.0
11	37.601548103245186	39.0	37.0	39.0	35.0	39.0
12	37.45882053576192	39.0	37.0	39.0	35.0	39.0
13	37.36500063615059	39.0	37.0	39.0	35.0	39.0
14	38.583029964744945	40.0	38.0	41.0	35.0	41.0
15	38.62709673182764	40.0	38.0	41.0	35.0	41.0
16	38.64945639905932	40.0	38.0	41.0	35.0	41.0
17	38.59922923173529	40.0	38.0	41.0	35.0	41.0
18	38.53709373576356	40.0	38.0	41.0	35.0	41.0
19	38.53329324900576	40.0	38.0	41.0	35.0	41.0
20	38.518768494539366	40.0	37.0	41.0	35.0	41.0
21	38.48315021772767	40.0	37.0	41.0	35.0	41.0
22	38.48104066028327	40.0	37.0	41.0	35.0	41.0
23	38.465003509088746	40.0	37.0	41.0	35.0	41.0
24	38.43962315259816	40.0	37.0	41.0	35.0	41.0
25	38.36253606563432	40.0	37.0	41.0	34.0	41.0
26	38.314968418201296	40.0	37.0	41.0	34.0	41.0
27	38.24880465251813	40.0	37.0	41.0	34.0	41.0
28	38.15598617706328	40.0	37.0	41.0	34.0	41.0
29	38.0855068478533	40.0	36.0	41.0	34.0	41.0
30	38.003886674902425	40.0	36.0	41.0	34.0	41.0
31	37.91567721308582	40.0	36.0	41.0	34.0	41.0
32	37.79213676827291	40.0	36.0	41.0	34.0	41.0
33	37.6923267926108	40.0	36.0	41.0	33.0	41.0
34	37.62033096247532	40.0	36.0	41.0	33.0	41.0
35	37.56740528538536	40.0	36.0	41.0	33.0	41.0
36	37.43719962405552	40.0	35.0	41.0	33.0	41.0
37	37.33349476509626	40.0	35.0	41.0	33.0	41.0
38	37.21817913179809	39.0	35.0	41.0	33.0	41.0
39	37.12435102379203	39.0	35.0	41.0	33.0	41.0
40	37.007605077712974	39.0	35.0	41.0	32.0	41.0
41	36.939918654808274	39.0	35.0	41.0	32.0	41.0
42	36.8449721530209	39.0	35.0	41.0	32.0	41.0
43	36.77565431166454	39.0	35.0	41.0	32.0	41.0
44	36.63629218601864	39.0	35.0	41.0	31.0	41.0
45	36.579089935276805	39.0	35.0	41.0	31.0	41.0
46	36.50403647810616	39.0	35.0	41.0	31.0	41.0
47	36.37011857026181	39.0	35.0	41.0	31.0	41.0
48	36.27572613511838	38.0	35.0	40.0	31.0	41.0
49	36.18619306965233	38.0	35.0	40.0	30.0	41.0
50	36.08953101336737	38.0	35.0	40.0	30.0	41.0
51	34.550011696962486	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	17.0
10	23.0
11	22.0
12	12.0
13	12.0
14	20.0
15	30.0
16	49.0
17	65.0
18	113.0
19	188.0
20	317.0
21	554.0
22	802.0
23	1216.0
24	1647.0
25	2274.0
26	2843.0
27	3411.0
28	3964.0
29	4696.0
30	5642.0
31	7078.0
32	9546.0
33	13562.0
34	29169.0
35	41266.0
36	32457.0
37	51876.0
38	98640.0
39	175720.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.29109840634017	21.98187586444657	25.200387436231033	11.526638292982232
2	30.2508075008311	25.056740528538533	26.427952867397487	18.264499103232875
3	32.27068823285574	24.900575818889976	26.608127131617504	16.220608816636776
4	29.36430087050026	25.03519349238466	27.210623304453463	18.389882332661614
5	25.534674311418286	29.885739145424022	24.578806745658785	20.0007797974989
6	26.40784230032054	34.475257846199305	23.27921265077795	15.837687202702202
7	79.23029882660998	5.765371245172438	9.304420630979303	5.699909297238285
8	78.84717200280727	5.0775077671935085	9.215769967946219	6.859550262053002
9	72.86653560596422	7.4952904335263675	11.69716769340004	7.941006267109373
10	40.74277763869109	25.224602200670628	18.375517641892365	15.657102518745921
11	30.559032722765572	24.786273922340378	23.444406594624322	21.210286760269728
12	27.151112442695148	22.18934304112816	30.372291742765327	20.287252773411367
13	23.71651487976754	25.326181085396033	31.127258847623462	19.830045187212963
14	20.20311672747719	28.95757491186236	29.119280287950488	21.720028072709958
15	19.04388618239874	25.75383845058341	33.74860970314341	21.453665663874443
16	20.90431884688471	25.541035817330382	30.165645405556262	23.388999930228646
17	20.85937788576377	25.621478085638184	26.98632891858504	26.532815110013008
18	21.73234066479789	25.33726241827517	29.88409746647897	23.046299450447975
19	22.801073658030067	26.41666632465022	26.34689496948529	24.43536504783442
20	23.983903337943715	27.309534460893154	26.903629341727786	21.802932859435344
21	23.48072874128371	27.29373330104698	28.120523859751366	21.10501409791794
22	21.780770193677075	25.368043898494992	26.08484196788055	26.766343939947383
23	21.20392525435763	25.689607761858053	27.68978834654201	25.416678637242306
24	22.816259188271847	25.338698887352095	28.049521245377644	23.79552067899841
25	21.68616844446816	26.37521393128753	26.490747087045925	25.44787053719839
26	20.295461168136654	26.720787349222046	28.627802653774015	24.355948828867284
27	22.490591127546143	25.669907614517367	29.495429976236696	22.344071281699797
28	20.99666328754417	25.355526096538934	29.501996692016924	24.145813923899972
29	21.176427132027925	23.238581096887785	31.642130406767	23.942861364317285
30	23.31389311849228	24.410945073526698	30.044982003094567	22.230179804886458
31	26.18334270458398	25.061255145637446	24.84701604330749	23.908386106471088
32	23.02700972284355	25.26687543350585	27.231965130739205	24.474149712911395
33	24.106824048954866	25.661494009923953	25.86218926095718	24.369492680164004
34	21.43376030666563	24.22420409352645	28.24631750891637	26.095718090891555
35	21.800470341017757	22.603866974755082	28.702704255642246	26.89295842858491
36	25.192794671110146	25.783593881462576	27.240378735332627	21.78323271209466
37	21.923391052028908	25.402724366209323	29.75871423705023	22.915170344711537
38	21.920312904006927	27.531161118475865	26.097154559968477	24.451371417548728
39	24.363541593988174	24.08794474108671	27.69184044522333	23.856673219701786
40	23.29070440339335	23.360475758558277	28.827266645598453	24.521553192449918
41	20.129856804554016	24.24267298165834	26.34155951291386	29.285910700873785
42	21.26815594308299	24.28084201713092	28.16977422810308	26.281227811683006
43	24.171259947548357	22.734996080491516	29.401443856632177	23.692300115327946
44	21.941244310556407	23.51397273992112	28.7790423265874	25.765740622935073
45	22.372185033633897	21.985159222336684	28.264991606916396	27.37766413711303
46	24.584552621966484	22.64655062732657	28.48046196845514	24.28843478225181
47	20.492462641543508	23.03255038928312	31.12664321801907	25.348343751154307
48	20.639392907126116	22.8983431355247	29.818635518544816	26.643628438804367
49	22.782399560030044	21.596081312358148	31.39074831830513	24.23077080930668
50	21.382047419896328	21.313096904203928	31.011315272128808	26.293540403770937
51	21.077310765720103	21.81976006862218	27.095500568431337	30.007428597226383
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	330.0
1	631.5
2	933.0
3	1286.0
4	1639.0
5	1274.5
6	910.0
7	855.0
8	800.0
9	796.0
10	792.0
11	836.0
12	880.0
13	900.0
14	920.0
15	950.0
16	980.0
17	1049.5
18	1119.0
19	1091.5
20	1064.0
21	1156.0
22	1248.0
23	1478.5
24	1709.0
25	2117.5
26	2956.5
27	3387.0
28	4278.0
29	5169.0
30	5371.5
31	5574.0
32	6361.0
33	7148.0
34	8642.5
35	10137.0
36	10324.5
37	10512.0
38	11931.0
39	13350.0
40	16453.5
41	19557.0
42	22839.5
43	26122.0
44	28799.0
45	31476.0
46	38588.5
47	45701.0
48	52978.5
49	60256.0
50	58374.0
51	56492.0
52	46625.5
53	36759.0
54	31308.0
55	25857.0
56	24043.0
57	22229.0
58	20946.5
59	19664.0
60	18519.0
61	17374.0
62	16426.0
63	15478.0
64	13372.5
65	11267.0
66	9885.0
67	8503.0
68	7580.0
69	6657.0
70	5397.5
71	4138.0
72	3731.0
73	3324.0
74	2821.0
75	1887.0
76	1456.0
77	1081.5
78	707.0
79	557.0
80	407.0
81	297.0
82	187.0
83	167.0
84	147.0
85	92.5
86	38.0
87	38.5
88	39.0
89	24.5
90	10.0
91	12.5
92	15.0
93	8.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	487306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.049575017743006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28666543511551	21.42134000757251
2	11.928820756155337	7.169119883705995
3	4.549161902966987	4.101011456131951
4	2.2264948080573745	2.6762089104534157
5	1.4023795010153761	2.1070454009553274
6	0.8701010832852	1.5687700665118844
7	0.7085921578149628	1.490502524227158
8	0.5343232049148903	1.2844948183848692
9	0.4480607176076386	1.2117630731629054
>10	5.339446273305003	38.769034697880386
>50	0.6220102928114608	11.32923226437702
>100	0.07706322211823408	4.0415465757524105
>500	0.004128386899191111	0.8553679942644766
>1k	0.002752257932794074	1.9745623266197128
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	3164	0.649284022770087	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	3159	0.6482579734294263	No Hit
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT	2100	0.4309407230774913	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	1127	0.23127152138492035	No Hit
ACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	889	0.18243157276947136	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	760	0.15595949978042545	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG	715	0.1467250557144792	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	715	0.1467250557144792	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	552	0.11327584720894057	No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG	506	0.10383619327486221	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG	496	0.10178409459354082	No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG	494	0.10137367485727652	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0520986813213875E-4	0.0	0.0	0.4572075861984051	0.0
2	2.0520986813213875E-4	0.0	0.0	2.276803486926079	0.0
3	2.0520986813213875E-4	0.0	0.0	3.102773206157938	0.0
4	2.0520986813213875E-4	0.0	0.0	3.946596183917292	0.0
5	2.0520986813213875E-4	0.0	0.0	6.262799965524742	0.0
6	2.0520986813213875E-4	0.0	0.0	7.413206486273512	0.0
7	2.0520986813213875E-4	0.0	0.0	8.64015628783557	0.0
8	2.0520986813213875E-4	0.0	0.0	10.44313018924454	0.0
9	2.0520986813213875E-4	0.0	0.0	11.039059646300272	0.0
10	2.0520986813213875E-4	0.0	0.0	12.42915129302738	0.0
11	2.0520986813213875E-4	0.0	0.0	14.439592371117943	0.0
12	2.0520986813213875E-4	0.0	0.0	16.047001268196986	0.0
13	2.0520986813213875E-4	0.0	0.0	16.64641929301096	0.0
14	2.0520986813213875E-4	0.0	0.0	16.877075184791487	0.0
15	2.0520986813213875E-4	0.0	0.0	17.345158894000896	0.0
16	2.0520986813213875E-4	0.0	0.0	18.298153521606547	0.0
17	2.0520986813213875E-4	0.0	0.0	19.417778562135496	0.0
18	2.0520986813213875E-4	0.0	0.0	20.6539628077635	0.0
19	2.0520986813213875E-4	0.0	0.0	21.368913988335873	0.0
20	2.0520986813213875E-4	0.0	0.0	22.0797609715456	0.0
21	2.0520986813213875E-4	0.0	0.0	22.928508986140127	0.0
22	2.0520986813213875E-4	0.0	0.0	23.785260185591806	0.0
23	2.0520986813213875E-4	0.0	0.0	24.658428174494055	0.0
24	2.0520986813213875E-4	0.0	0.0	25.37009599717631	0.0
25	2.0520986813213875E-4	0.0	0.0	25.947146146363885	0.0
26	2.0520986813213875E-4	0.0	0.0	26.515167061353647	0.0
27	2.0520986813213875E-4	0.0	0.0	27.059178421771946	0.0
28	2.0520986813213875E-4	0.0	0.0	27.619196151904553	0.0
29	2.0520986813213875E-4	0.0	0.0	28.22559131223502	0.0
30	2.0520986813213875E-4	0.0	0.0	28.894370272477662	0.0
31	4.104197362642775E-4	0.0	0.0	29.45951824931357	0.0
32	4.104197362642775E-4	0.0	0.0	30.0049660788088	0.0
33	4.104197362642775E-4	0.0	0.0	30.55431289579853	0.0
34	4.104197362642775E-4	0.0	0.0	31.082728306238792	0.0
35	4.104197362642775E-4	0.0	0.0	31.66039408503076	0.0
36	4.104197362642775E-4	0.0	0.0	32.16931455799846	0.0
37	4.104197362642775E-4	0.0	0.0	32.68028712964749	0.0
38	4.104197362642775E-4	0.0	0.0	33.1949534789229	0.0
39	4.104197362642775E-4	0.0	0.0	33.677401878901556	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	25	3.8874874E-5	45.0	15
TATCACG	20	7.0292834E-4	45.0	1
TTAGGAT	25	3.8874874E-5	45.0	32
GTCGCTA	20	7.0292834E-4	45.0	22
AGATCGA	20	7.0292834E-4	45.0	17
CGAACGT	20	7.0292834E-4	45.0	38
GTATTAG	55	1.8189894E-12	45.0	1
AATCACG	20	7.0292834E-4	45.0	2
CAAGTAA	20	7.0292834E-4	45.0	26
CGCATGG	40	6.8012014E-9	45.0	2
TACGGTA	20	7.0292834E-4	45.0	19
TCGATAG	25	3.8874874E-5	45.0	1
CCCGTTA	25	3.8874874E-5	45.0	43
AATGCGA	35	1.21001E-7	45.0	25
CGGATAT	25	3.8874874E-5	45.0	9
TACGACG	35	1.21001E-7	45.0	1
CGCTAAT	20	7.0292834E-4	45.0	29
ACATGCG	25	3.8874874E-5	45.0	1
ACGTCTT	20	7.0292834E-4	45.0	23
ACGTCGC	25	3.8874874E-5	45.0	20
>>END_MODULE