FastQCFastQC Report
Sat 18 Jun 2016
SRR3550218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786518
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC28480.3621023295080341No Hit
CCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC23230.2953524267721781No Hit
CTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGCT16720.21258254737971669Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.12409124775275328No Hit
TCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC9240.11747982881510659No Hit
ACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC8180.10400270559605757No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGA253.8896782E-545.017
AACCCGC207.0319267E-445.021
TTATGCG253.8896782E-545.01
GAGTACG253.8896782E-545.01
CACGAGG253.8896782E-545.02
ATCACGA207.0319267E-445.021
CACCTCG253.8896782E-545.018
CTACGCG207.0319267E-445.01
TACGAAG551.8189894E-1245.01
AATTACG253.8896782E-545.01
CTCGACC207.0319267E-445.012
ATAACGC406.8102963E-945.011
TACGTTG253.8896782E-545.01
AAATGCG207.0319267E-445.01
CCGTAAG302.1646247E-644.9999961
ACATACG2050.043.9024417
CGTAAGG800.042.18752
ATACGAA2200.040.90909219
AATACGG556.002665E-1140.9090922
CGCATGG1000.040.52