##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550218_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	786518
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50390963716024	33.0	31.0	34.0	31.0	34.0
2	32.67118743626973	34.0	31.0	34.0	31.0	34.0
3	32.65633590076769	34.0	31.0	34.0	31.0	34.0
4	36.15034748092224	37.0	35.0	37.0	35.0	37.0
5	36.16202044962734	37.0	35.0	37.0	35.0	37.0
6	36.176693985388766	37.0	36.0	37.0	35.0	37.0
7	36.271185147701644	37.0	37.0	37.0	35.0	37.0
8	36.145785601855266	37.0	37.0	37.0	35.0	37.0
9	37.68773632644135	39.0	38.0	39.0	35.0	39.0
10	37.69199433452254	39.0	37.0	39.0	35.0	39.0
11	37.61967558275844	39.0	37.0	39.0	35.0	39.0
12	37.44235224114388	39.0	37.0	39.0	35.0	39.0
13	37.37296794224671	39.0	37.0	39.0	35.0	39.0
14	38.60378402020043	40.0	38.0	41.0	35.0	41.0
15	38.64562031638182	40.0	38.0	41.0	35.0	41.0
16	38.67142137878599	40.0	38.0	41.0	35.0	41.0
17	38.609570283197584	40.0	38.0	41.0	35.0	41.0
18	38.56674100274883	40.0	38.0	41.0	35.0	41.0
19	38.53969267073354	40.0	38.0	41.0	35.0	41.0
20	38.54558191929492	40.0	38.0	41.0	35.0	41.0
21	38.494855807495824	40.0	38.0	41.0	35.0	41.0
22	38.49349538090673	40.0	37.0	41.0	35.0	41.0
23	38.47710287622152	40.0	37.0	41.0	35.0	41.0
24	38.44197335598168	40.0	37.0	41.0	35.0	41.0
25	38.35504209693866	40.0	37.0	41.0	34.0	41.0
26	38.282864473540336	40.0	37.0	41.0	34.0	41.0
27	38.22125876330866	40.0	37.0	41.0	34.0	41.0
28	38.106240416621105	40.0	37.0	41.0	34.0	41.0
29	38.06115181089307	40.0	36.0	41.0	34.0	41.0
30	37.97810476047592	40.0	36.0	41.0	34.0	41.0
31	37.878407105749645	40.0	36.0	41.0	34.0	41.0
32	37.79034936263378	40.0	36.0	41.0	34.0	41.0
33	37.68237980567514	40.0	36.0	41.0	33.0	41.0
34	37.532673123819166	40.0	35.0	41.0	33.0	41.0
35	37.48595200618422	40.0	35.0	41.0	33.0	41.0
36	37.415610322967815	40.0	35.0	41.0	33.0	41.0
37	37.3235132571664	40.0	35.0	41.0	33.0	41.0
38	37.23346064552877	40.0	35.0	41.0	33.0	41.0
39	37.13700894321554	39.0	35.0	41.0	33.0	41.0
40	36.989892157585714	39.0	35.0	41.0	32.0	41.0
41	36.89713649274397	39.0	35.0	41.0	32.0	41.0
42	36.81251160176881	39.0	35.0	41.0	31.0	41.0
43	36.744733114817464	39.0	35.0	41.0	31.0	41.0
44	36.666320414790256	39.0	35.0	41.0	31.0	41.0
45	36.58867565650119	39.0	35.0	41.0	31.0	41.0
46	36.51317833794013	39.0	35.0	41.0	31.0	41.0
47	36.40682349291434	39.0	35.0	41.0	31.0	41.0
48	36.31760366577752	38.0	35.0	40.0	31.0	41.0
49	36.225915999379545	38.0	35.0	40.0	31.0	41.0
50	36.09674540188527	38.0	35.0	40.0	30.0	41.0
51	34.634546189661265	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	15.0
9	26.0
10	28.0
11	18.0
12	21.0
13	20.0
14	23.0
15	40.0
16	79.0
17	134.0
18	232.0
19	330.0
20	613.0
21	882.0
22	1389.0
23	1953.0
24	2755.0
25	3680.0
26	4700.0
27	5602.0
28	6219.0
29	7253.0
30	9166.0
31	11611.0
32	15280.0
33	22015.0
34	47322.0
35	67173.0
36	51428.0
37	81150.0
38	155470.0
39	289735.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63909789731449	21.63218133596434	25.653195476772307	11.075525289948864
2	31.769902278142393	23.864806654138874	25.783898143462707	18.581392924256022
3	31.552106881215686	24.190037608802342	26.83206233042346	17.42579317955851
4	28.413208597896045	26.453304310899433	26.57815841468345	18.55532867652107
5	25.507744260144076	29.443191382778274	24.947045077162887	20.102019279914764
6	26.63944118252856	34.87053061722681	23.19997762288975	15.290050577354872
7	81.74879659461067	5.545581919294918	7.761805832797215	4.94381565329719
8	82.82772930816587	4.513437708990767	7.391057801601489	5.267775181241879
9	76.6323211929034	7.092399665360488	9.555788932993268	6.7194902087428385
10	42.04417444991723	26.517129932182097	16.132879349232947	15.305816268667725
11	32.69550092941293	25.224216101856538	22.401903071512667	19.678379897217866
12	30.36866289137693	22.088242099990083	28.1870217846254	19.356073224007588
13	25.54779420178559	24.935475094022006	28.631766850854017	20.884963853338384
14	20.92793807643309	27.363518698872753	28.232284575813903	23.476258648880254
15	19.439097388743807	25.840603775120215	33.39643847947536	21.323860356660624
16	23.020070742182632	24.293150315695257	30.32645152431349	22.36032741780862
17	22.18347196122657	24.151131951207727	28.386000066114192	25.27939602145151
18	23.814203870731504	24.983280738648066	28.75763809601306	22.44487729460737
19	24.216991855240437	27.538848443392265	25.959355030654095	22.284804670713193
20	26.900083659878092	25.83907806305768	26.0350049204214	21.225833356642823
21	25.74219534708678	26.866390851830474	26.90936507492518	20.48204872615757
22	23.27244894586011	25.4715085986589	26.35057303202215	24.90546942345884
23	23.308938892689042	26.454830022961968	26.292214545630234	23.944016538718756
24	24.455130079667597	24.2906074622577	27.845007997274063	23.409254460800643
25	23.11072346723152	26.012246382155276	25.93163792818473	24.945392222428474
26	21.562507151775293	26.99493209309895	27.109996211148378	24.332564543977377
27	22.32930460587043	26.523487065775992	26.469832858243546	24.67737547011003
28	19.91562812294188	27.727401025787074	28.16947609590626	24.187494755364785
29	21.323224643301234	25.708248253695405	28.719876722465347	24.248650380538017
30	23.173277661795407	25.277361738701465	27.153479004930592	24.395881594572533
31	23.361067388158947	28.04233342402844	25.345383068156103	23.25121611965651
32	25.217858968262647	26.543067037245173	25.836280924276366	22.40279307021581
33	24.829183820332148	25.499225701128264	25.069102042165596	24.602488436373992
34	21.605354232198117	26.126420501501556	28.948860674517302	23.319364591783025
35	22.535275734312503	24.26428892917899	28.246397412392344	24.954037924116168
36	23.20290190434294	27.25417600105783	25.785805283540874	23.75711681105836
37	22.850970988585132	28.02542344866869	27.054307720865893	22.069297841880285
38	22.88542665266402	27.401534357764223	26.05827202937504	23.654766960196717
39	23.80606673973132	24.813799557034933	27.253540287698435	24.126593415535307
40	24.517811416903363	23.243206131328208	29.211664577288758	23.02731787447967
41	21.77089399098304	25.55033705522315	27.52867702964204	25.15009192415177
42	23.401625900487975	25.774489585743748	26.20270610462825	24.621178409140033
43	23.110850609903398	24.55824278656051	27.359450133372665	24.971456470163428
44	21.926643764033372	24.45042580080812	27.796185211272977	25.826745223885535
45	22.410167345184725	24.221823276771797	26.7458595988903	26.622149779153183
46	22.64563557350245	25.87989086073046	27.121820479633012	24.352653086134072
47	21.69918552404395	25.22167324841898	28.75852809471621	24.320613132820863
48	21.345856038895487	23.276136083344564	29.875985037850374	25.50202283990958
49	23.225533299937194	23.211293320686877	29.55660264609329	24.006570733282643
50	20.480141586079405	24.058572086080673	29.24726452541455	26.214021802425375
51	20.339776076326288	23.158783397201336	28.267757381267817	28.23368314520456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	327.0
1	863.5
2	1400.0
3	1882.5
4	2365.0
5	1889.5
6	1414.0
7	1254.5
8	1095.0
9	1142.5
10	1190.0
11	1244.0
12	1298.0
13	1403.5
14	1509.0
15	1478.5
16	1448.0
17	1492.0
18	1536.0
19	1705.5
20	1875.0
21	1860.5
22	1846.0
23	2032.0
24	2218.0
25	3001.5
26	4543.5
27	5302.0
28	7118.0
29	8934.0
30	9894.5
31	10855.0
32	11956.5
33	13058.0
34	15295.5
35	17533.0
36	20004.0
37	22475.0
38	23195.5
39	23916.0
40	28227.0
41	32538.0
42	36408.5
43	40279.0
44	46101.0
45	51923.0
46	58259.5
47	64596.0
48	71737.5
49	78879.0
50	77553.0
51	76227.0
52	66714.0
53	57201.0
54	50603.5
55	44006.0
56	42070.0
57	40134.0
58	40336.5
59	40539.0
60	38431.5
61	36324.0
62	33697.0
63	31070.0
64	27252.0
65	23434.0
66	19295.5
67	15157.0
68	12425.0
69	9693.0
70	8291.5
71	6890.0
72	6045.0
73	5200.0
74	4153.0
75	2427.5
76	1749.0
77	1418.0
78	1087.0
79	742.0
80	397.0
81	368.0
82	339.0
83	277.0
84	215.0
85	163.0
86	111.0
87	66.0
88	21.0
89	16.0
90	11.0
91	6.5
92	2.0
93	4.0
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	786518.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.800620273974737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28216905568318	20.094891344962956
2	10.987063766229022	6.108943753817597
3	4.10066253532272	3.4200288604866436
4	2.037004375055757	2.265199405094013
5	1.2197073092779362	1.6954309875313682
6	0.8527341067534571	1.4223922257911936
7	0.6415693230053684	1.2485217589812303
8	0.5362027347171917	1.1925414894191566
9	0.4332022655231846	1.0838962517072068
>10	5.8188255338353025	38.89265565664225
>50	1.01721450551514	17.884990832517346
>100	0.06950198657090399	3.231761668733288
>500	0.0027616683405657216	0.5831144252620207
>1k	0.0013808341702828608	0.8756313390537651
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	2848	0.3621023295080341	No Hit
CCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	2323	0.2953524267721781	No Hit
CTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGCT	1672	0.21258254737971669	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	976	0.12409124775275328	No Hit
TCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	924	0.11747982881510659	No Hit
ACTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC	818	0.10400270559605757	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5428534375564193E-4	0.0	0.0	0.23076394945824508	0.0
2	2.5428534375564193E-4	0.0	0.0	1.1878939833544815	0.0
3	2.5428534375564193E-4	0.0	0.0	1.6388690405051125	0.0
4	2.5428534375564193E-4	0.0	0.0	2.159263996501034	0.0
5	2.5428534375564193E-4	0.0	0.0	3.5345662782034233	0.0
6	2.5428534375564193E-4	0.0	0.0	4.347897950205844	0.0
7	2.5428534375564193E-4	0.0	0.0	5.114568261629104	0.0
8	2.5428534375564193E-4	0.0	0.0	6.290510833827071	0.0
9	2.5428534375564193E-4	0.0	0.0	6.759921578399986	0.0
10	3.8142801563346296E-4	0.0	0.0	7.7985500649699055	0.0
11	3.8142801563346296E-4	0.0	0.0	9.377407764348686	0.0
12	3.8142801563346296E-4	0.0	0.0	10.57165888129706	0.0
13	3.8142801563346296E-4	0.0	0.0	10.997841117431514	0.0
14	3.8142801563346296E-4	0.0	0.0	11.168848011107183	0.0
15	3.8142801563346296E-4	0.0	0.0	11.536417475505964	0.0
16	3.8142801563346296E-4	0.0	0.0	12.258969279787621	0.0
17	3.8142801563346296E-4	0.0	0.0	13.126209444666237	0.0
18	3.8142801563346296E-4	0.0	0.0	14.091349466890776	0.0
19	3.8142801563346296E-4	0.0	0.0	14.646327229637466	0.0
20	5.085706875112839E-4	0.0	0.0	15.201940705743542	0.0
21	5.085706875112839E-4	0.0	0.0	15.908599676040472	0.0
22	5.085706875112839E-4	0.0	0.0	16.59237296539939	0.0
23	5.085706875112839E-4	0.0	0.0	17.313399057618515	0.0
24	5.085706875112839E-4	0.0	0.0	17.89889106161588	0.0
25	5.085706875112839E-4	0.0	0.0	18.39957890347074	0.0
26	5.085706875112839E-4	0.0	0.0	18.846358252449402	0.0
27	5.085706875112839E-4	0.0	0.0	19.275210484693293	0.0
28	5.085706875112839E-4	0.0	0.0	19.73114410604716	0.0
29	5.085706875112839E-4	0.0	0.0	20.19839342519815	0.0
30	5.085706875112839E-4	0.0	0.0	20.7335369311319	0.0
31	5.085706875112839E-4	0.0	0.0	21.219857651064565	0.0
32	5.085706875112839E-4	0.0	0.0	21.686598399528048	0.0
33	5.085706875112839E-4	0.0	0.0	22.1781319690077	0.0
34	5.085706875112839E-4	0.0	0.0	22.64347414808053	0.0
35	5.085706875112839E-4	0.0	0.0	23.14797627009172	0.0
36	5.085706875112839E-4	0.0	0.0	23.607469886258166	0.0
37	5.085706875112839E-4	0.0	0.0	24.105487732003592	0.0
38	5.085706875112839E-4	0.0	0.0	24.60553986049906	0.0
39	5.085706875112839E-4	0.0	0.0	25.07469631972822	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGA	25	3.8896782E-5	45.0	17
AACCCGC	20	7.0319267E-4	45.0	21
TTATGCG	25	3.8896782E-5	45.0	1
GAGTACG	25	3.8896782E-5	45.0	1
CACGAGG	25	3.8896782E-5	45.0	2
ATCACGA	20	7.0319267E-4	45.0	21
CACCTCG	25	3.8896782E-5	45.0	18
CTACGCG	20	7.0319267E-4	45.0	1
TACGAAG	55	1.8189894E-12	45.0	1
AATTACG	25	3.8896782E-5	45.0	1
CTCGACC	20	7.0319267E-4	45.0	12
ATAACGC	40	6.8102963E-9	45.0	11
TACGTTG	25	3.8896782E-5	45.0	1
AAATGCG	20	7.0319267E-4	45.0	1
CCGTAAG	30	2.1646247E-6	44.999996	1
ACATACG	205	0.0	43.90244	17
CGTAAGG	80	0.0	42.1875	2
ATACGAA	220	0.0	40.909092	19
AATACGG	55	6.002665E-11	40.909092	2
CGCATGG	100	0.0	40.5	2
>>END_MODULE