SRR3550212_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503070
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	5957	1.1841294452064326	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	4848	0.9636829864631165	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	3459	0.6875782694257261	TruSeq Adapter, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	2211	0.4395014610292802	TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1513	0.30075337428190907	TruSeq Adapter, Index 16 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	1019	0.20255630429164925	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	931	0.18506370882779732	TruSeq Adapter, Index 16 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	870	0.1729381596994454	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	861	0.1711491442542787	TruSeq Adapter, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	760	0.15107241536963048	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	671	0.1333810404118711	No Hit
GGCCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	608	0.12085793229570437	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	586	0.11648478342974138	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	566	0.11250919355159322	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	520	0.10336533683185242	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	507	0.10078120341105612	No Hit
GACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	505	0.1003836444232413	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	20	7.0294994E-4	45.000004	20
CGGCGTT	20	7.0294994E-4	45.000004	42
TGTCAAC	20	7.0294994E-4	45.000004	12
TTAGGCG	20	7.0294994E-4	45.000004	1
ACAGTTC	20	7.0294994E-4	45.000004	12
CGGCGAA	20	7.0294994E-4	45.000004	40
TCGTTGA	30	2.1630585E-6	45.000004	24
CTGTCGA	20	7.0294994E-4	45.000004	11
TGCCCGT	20	7.0294994E-4	45.000004	13
CGAACGG	20	7.0294994E-4	45.000004	3
ACACGAA	20	7.0294994E-4	45.000004	12
ATTAGCG	20	7.0294994E-4	45.000004	1
TTTACGG	30	2.1630585E-6	45.000004	2
CTATCGA	30	2.1630585E-6	45.000004	1
GCGAAGT	20	7.0294994E-4	45.000004	42
GCGAACG	20	7.0294994E-4	45.000004	2
TTCACGG	20	7.0294994E-4	45.000004	2
TGAACGT	20	7.0294994E-4	45.000004	45
CACGATT	30	2.1630585E-6	45.000004	24
AAGCGCT	20	7.0294994E-4	45.000004	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content