##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503070
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25716103126801	33.0	31.0	34.0	30.0	34.0
2	32.40180293000974	34.0	31.0	34.0	30.0	34.0
3	32.413407676864054	34.0	31.0	34.0	30.0	34.0
4	35.97343510843422	37.0	35.0	37.0	35.0	37.0
5	35.98812690082891	37.0	35.0	37.0	35.0	37.0
6	35.99629475023357	37.0	35.0	37.0	35.0	37.0
7	36.145216371479115	37.0	35.0	37.0	35.0	37.0
8	35.969135507980994	37.0	35.0	37.0	35.0	37.0
9	37.52282982487527	39.0	38.0	39.0	35.0	39.0
10	37.55329874570139	39.0	37.0	39.0	35.0	39.0
11	37.492458306001154	39.0	37.0	39.0	35.0	39.0
12	37.36734649253583	39.0	37.0	39.0	35.0	39.0
13	37.25629634046952	39.0	37.0	39.0	34.0	39.0
14	38.35570994096249	40.0	38.0	41.0	34.0	41.0
15	38.4288548313356	40.0	38.0	41.0	34.0	41.0
16	38.47213509054406	40.0	38.0	41.0	34.0	41.0
17	38.43072733416821	40.0	37.0	41.0	34.0	41.0
18	38.39761265827817	40.0	37.0	41.0	34.0	41.0
19	38.424378317132806	40.0	37.0	41.0	34.0	41.0
20	38.41417894130041	40.0	37.0	41.0	34.0	41.0
21	38.33404695171646	40.0	37.0	41.0	34.0	41.0
22	38.373218438785855	40.0	37.0	41.0	34.0	41.0
23	38.39514779255372	40.0	37.0	41.0	34.0	41.0
24	38.37026855109627	40.0	37.0	41.0	34.0	41.0
25	38.286162959429106	40.0	37.0	41.0	34.0	41.0
26	38.25797801498798	40.0	37.0	41.0	34.0	41.0
27	38.18372989842368	40.0	37.0	41.0	34.0	41.0
28	38.11908481921005	40.0	37.0	41.0	34.0	41.0
29	38.07715427276522	40.0	37.0	41.0	34.0	41.0
30	37.993358777108554	40.0	37.0	41.0	34.0	41.0
31	37.90485220744628	40.0	36.0	41.0	34.0	41.0
32	37.83297155465442	40.0	36.0	41.0	34.0	41.0
33	37.758697596755916	40.0	36.0	41.0	33.0	41.0
34	37.700930288031486	40.0	36.0	41.0	33.0	41.0
35	37.63474665553502	40.0	36.0	41.0	33.0	41.0
36	37.54218498419703	40.0	36.0	41.0	33.0	41.0
37	37.485930387421234	40.0	36.0	41.0	33.0	41.0
38	37.3711551076391	40.0	35.0	41.0	33.0	41.0
39	37.27820581628799	40.0	35.0	41.0	33.0	41.0
40	37.129232512374024	39.0	35.0	41.0	32.0	41.0
41	37.04022700618204	39.0	35.0	41.0	32.0	41.0
42	37.00263979167909	39.0	35.0	41.0	32.0	41.0
43	36.929238475758844	39.0	35.0	41.0	32.0	41.0
44	36.81619456536863	39.0	35.0	41.0	31.0	41.0
45	36.74163237720397	39.0	35.0	41.0	31.0	41.0
46	36.64123084262627	39.0	35.0	41.0	31.0	41.0
47	36.52808356689924	39.0	35.0	41.0	31.0	41.0
48	36.49399288369412	39.0	35.0	41.0	31.0	41.0
49	36.4094877452442	39.0	35.0	40.0	31.0	41.0
50	36.268555071858785	39.0	35.0	40.0	30.0	41.0
51	34.64949609398295	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	14.0
10	15.0
11	18.0
12	7.0
13	5.0
14	4.0
15	25.0
16	36.0
17	70.0
18	123.0
19	226.0
20	375.0
21	599.0
22	892.0
23	1324.0
24	1795.0
25	2459.0
26	2878.0
27	3461.0
28	3961.0
29	4942.0
30	6260.0
31	8035.0
32	10398.0
33	14821.0
34	29245.0
35	41552.0
36	33210.0
37	51025.0
38	102427.0
39	182788.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26217027451448	21.40616613990101	23.92788279961039	11.403780785974119
2	30.990915777128432	24.704514282306636	24.70670085673962	19.597869083825312
3	29.63484206969209	23.796489554137594	28.592442403641638	17.976225972528674
4	28.411553064185895	25.33086846760888	26.446418987417257	19.811159480787964
5	23.887133003359374	28.049575605780507	26.779374639712167	21.283916751147952
6	24.764148130478862	32.06929453157612	26.296141690023255	16.87041564792176
7	74.91661200230584	5.84948416721331	12.202874351481901	7.031029478998946
8	70.99409624903095	5.4757787186673825	12.818494444113146	10.711630588188523
9	65.12055976305484	7.009958852644761	16.959071302204464	10.91041008209593
10	36.89387162820283	22.494682648537978	23.024628779295128	17.586816943964063
11	26.814161051145962	25.064305166279045	26.67223249249607	21.449301290078914
12	26.420975212197114	19.98707933289602	33.16794879440237	20.423996660504503
13	22.87435148190113	25.937742262508202	32.212415767189455	18.975490488401217
14	16.10193412447572	30.271334009183615	29.911940684199017	23.71479118214165
15	14.554634543900452	26.436480012721887	38.749080644840674	20.259804798536983
16	15.15912298487288	26.135925417933887	33.343669867016516	25.361281730176717
17	15.480748206015068	26.35438408173813	29.25795614924364	28.906911563003163
18	16.444431192478184	25.656469278629217	34.08253324587036	23.816566283022244
19	18.814677877830125	27.539507424414094	28.674140775637586	24.971673922118196
20	21.75263879778162	27.73828691832151	29.72528673941996	20.783787544476912
21	19.947323434114537	27.973244280120063	30.992903572067505	21.086528713697895
22	16.97557000019878	26.993658934144353	26.617568131671536	29.41320293398533
23	17.137177728745502	27.60848390879997	28.92996998429642	26.32436837815811
24	21.68068062098714	23.813584590613633	29.318981453873217	25.186753334526014
25	17.659371459240266	27.560180491780468	27.02844534557815	27.752002703401118
26	17.621802134891766	27.68879082433856	30.451428230663723	24.23797881010595
27	22.962609577196016	26.445425089947722	26.992068698193094	23.599896634663168
28	17.042757469139485	25.26805414753414	29.58713499115431	28.102053392172067
29	18.52108056532888	22.31955791440555	32.82107857753394	26.338282942731627
30	23.408074423042518	23.240503309678573	26.618363249647164	26.73305901763174
31	19.37404337368557	24.85240622577375	25.08557457212714	30.68797582841354
32	20.606873794899318	23.591547895919057	30.959111058103243	24.84246725107838
33	21.83195181585068	22.006281432007473	27.48802353549208	28.673743216649772
34	16.367901087323833	20.804460611843282	31.663188025523286	31.164450275309598
35	17.965690659351583	19.19752718309579	34.17973641839108	28.657045739161546
36	20.740453614805094	21.691215934164234	31.130458981851433	26.437871469179242
37	18.8677520027034	21.57015922237462	34.222672789075084	25.3394159858469
38	18.052159739201304	22.99381795773948	31.397817401156896	27.55620490190232
39	19.55592661061085	19.487944023694514	33.70167173554376	27.254457630150874
40	21.332021388673546	18.972508795992603	34.02945912099708	25.666010694336773
41	17.213111495418133	21.230842626274672	32.24143757329993	29.314608305007255
42	20.599121394636928	19.817321645099092	34.44808873516608	25.135468225097902
43	23.365734390840238	19.788101059494704	32.937563360963686	23.908601188701372
44	19.46468682290735	20.904645476772615	30.24231220307313	29.388355497246906
45	19.986880553402113	18.71290277694953	29.971972091359056	31.328244578289304
46	22.387341721827976	19.704414892559683	31.348321307173954	26.559922078438387
47	16.690520205935556	20.571292265489895	36.28898562824259	26.449201900331964
48	17.944818812491302	18.06170115490886	34.42741566779971	29.566064364800127
49	20.259804798536983	16.70264575506391	37.00359790883972	26.033951537559386
50	19.51358657840857	16.834635339018426	34.128451308962966	29.523326773610037
51	17.38585087562367	17.089669429701633	29.736617170572682	35.78786252410202
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	442.0
1	583.5
2	725.0
3	1055.5
4	1386.0
5	1043.0
6	700.0
7	736.0
8	772.0
9	818.0
10	864.0
11	882.5
12	901.0
13	1029.0
14	1157.0
15	1147.0
16	1137.0
17	1037.5
18	938.0
19	1223.0
20	1508.0
21	1346.0
22	1184.0
23	1405.0
24	1626.0
25	1688.5
26	2244.5
27	2738.0
28	3163.0
29	3588.0
30	4773.5
31	5959.0
32	6544.5
33	7130.0
34	8489.0
35	9848.0
36	10593.0
37	11338.0
38	12880.5
39	14423.0
40	16348.5
41	18274.0
42	21911.0
43	25548.0
44	31619.0
45	37690.0
46	47464.5
47	57239.0
48	67725.0
49	78211.0
50	76638.5
51	75066.0
52	58280.5
53	41495.0
54	33558.5
55	25622.0
56	21896.5
57	18171.0
58	16389.0
59	14607.0
60	13256.0
61	11905.0
62	10722.5
63	9540.0
64	8173.5
65	6807.0
66	5771.0
67	4735.0
68	3806.0
69	2877.0
70	2542.0
71	2207.0
72	1711.5
73	1216.0
74	1092.0
75	681.5
76	395.0
77	293.5
78	192.0
79	143.5
80	95.0
81	63.5
82	32.0
83	25.5
84	19.0
85	16.0
86	13.0
87	16.5
88	20.0
89	10.5
90	1.0
91	2.5
92	4.0
93	4.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	503070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.082668888464912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.12657581726937	19.522199781100813
2	13.202224656985079	7.679118565804487
3	5.806456272507455	5.066017355660534
4	3.1753734677730523	3.693933406418412
5	1.9616131935938468	2.852447349826703
6	1.2599000438943688	2.19847534854854
7	0.8780140703365388	1.7874494740907625
8	0.6402109851988522	1.4895235281036903
9	0.5198796964117067	1.3607540165319596
>10	4.6564516262694555	31.06088381146564
>50	0.6602687723978101	12.203933086717976
>100	0.10131472812367234	5.7951895114262975
>500	0.007581374213336024	1.4802668283717213
>1k	0.0034460791878800114	2.61577279759661
>5k	6.892158375760022E-4	1.1940351383358623
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	5957	1.1841294452064326	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	4848	0.9636829864631165	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	3459	0.6875782694257261	TruSeq Adapter, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	2211	0.4395014610292802	TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1513	0.30075337428190907	TruSeq Adapter, Index 16 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	1019	0.20255630429164925	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	931	0.18506370882779732	TruSeq Adapter, Index 16 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	870	0.1729381596994454	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	861	0.1711491442542787	TruSeq Adapter, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	760	0.15107241536963048	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	671	0.1333810404118711	No Hit
GGCCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	608	0.12085793229570437	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	586	0.11648478342974138	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	566	0.11250919355159322	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	520	0.10336533683185242	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	507	0.10078120341105612	No Hit
GACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	505	0.1003836444232413	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.737670701890393	0.0
2	0.0	0.0	0.0	3.843997853181466	0.0
3	0.0	0.0	0.0	5.241815254338363	0.0
4	0.0	0.0	0.0	6.814359830639871	0.0
5	0.0	0.0	0.0	10.776830262190153	0.0
6	0.0	0.0	0.0	12.728646112866997	0.0
7	0.0	0.0	0.0	14.714254477508101	0.0
8	0.0	0.0	0.0	17.945415150973027	0.0
9	0.0	0.0	0.0	19.058977875842327	0.0
10	0.0	0.0	0.0	21.64311129663864	0.0
11	0.0	0.0	0.0	25.073647802492694	0.0
12	0.0	0.0	0.0	27.660961695191524	0.0
13	0.0	0.0	0.0	28.716282028345955	0.0
14	0.0	0.0	0.0	29.116623929075477	0.0
15	0.0	0.0	0.0	29.856679984892757	0.0
16	0.0	0.0	0.0	31.409545391297435	0.0
17	0.0	0.0	0.0	33.06358956010098	0.0
18	0.0	0.0	0.0	35.15634007195818	0.0
19	0.0	0.0	0.0	36.17250084481285	0.0
20	0.0	0.0	0.0	37.189655515137055	0.0
21	0.0	0.0	0.0	38.36921303198362	0.0
22	0.0	0.0	0.0	39.56228755441589	0.0
23	0.0	0.0	0.0	40.71023913173117	0.0
24	0.0	0.0	0.0	41.58347744846642	0.0
25	0.0	0.0	0.0	42.31657622199694	0.0
26	0.0	0.0	0.0	42.944918202238256	0.0
27	0.0	0.0	0.0	43.584988172620115	0.0
28	0.0	0.0	0.0	44.223269127556804	0.0
29	0.0	0.0	0.0	44.90567913014093	0.0
30	0.0	0.0	0.0	45.651897350269344	0.0
31	0.0	0.0	0.0	46.36054624604926	0.0
32	1.9877949390740852E-4	0.0	0.0	47.036396525334446	0.0
33	1.9877949390740852E-4	0.0	0.0	47.56992068698193	0.0
34	1.9877949390740852E-4	0.0	0.0	48.17460790744827	0.0
35	1.9877949390740852E-4	0.0	0.0	48.77015127119486	0.0
36	1.9877949390740852E-4	0.0	0.0	49.37444093267339	0.0
37	1.9877949390740852E-4	0.0	0.0	49.96680382451746	0.0
38	1.9877949390740852E-4	0.0	0.0	50.49178046792693	0.0
39	1.9877949390740852E-4	0.0	0.0	51.05015206631284	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	20	7.0294994E-4	45.000004	20
CGGCGTT	20	7.0294994E-4	45.000004	42
TGTCAAC	20	7.0294994E-4	45.000004	12
TTAGGCG	20	7.0294994E-4	45.000004	1
ACAGTTC	20	7.0294994E-4	45.000004	12
CGGCGAA	20	7.0294994E-4	45.000004	40
TCGTTGA	30	2.1630585E-6	45.000004	24
CTGTCGA	20	7.0294994E-4	45.000004	11
TGCCCGT	20	7.0294994E-4	45.000004	13
CGAACGG	20	7.0294994E-4	45.000004	3
ACACGAA	20	7.0294994E-4	45.000004	12
ATTAGCG	20	7.0294994E-4	45.000004	1
TTTACGG	30	2.1630585E-6	45.000004	2
CTATCGA	30	2.1630585E-6	45.000004	1
GCGAAGT	20	7.0294994E-4	45.000004	42
GCGAACG	20	7.0294994E-4	45.000004	2
TTCACGG	20	7.0294994E-4	45.000004	2
TGAACGT	20	7.0294994E-4	45.000004	45
CACGATT	30	2.1630585E-6	45.000004	24
AAGCGCT	20	7.0294994E-4	45.000004	16
>>END_MODULE