FastQCFastQC Report
Sat 18 Jun 2016
SRR3550211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377392
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC36320.9623945393649044No Hit
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC24630.6526370458303302No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC20870.5530058930766948No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG19350.5127294696230975No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC18910.5010705049391614No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT17850.472982999109679TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC10800.2861745876966125No Hit
ACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC8340.22099037605460636TruSeq Adapter, Index 15 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG5940.1573960232331369No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.15368635265188452No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT5700.15103658795098995No Hit
CGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG5250.13911264679696442TruSeq Adapter, Index 15 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG4910.1301034468139229No Hit
AGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG4600.12188917624114978TruSeq Adapter, Index 15 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG4590.12162419977106032TruSeq Adapter, Index 15 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4390.11632467036927122No Hit
GAATGACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT4050.1073154703862297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTT302.1616124E-645.00000410
TACCAAT1200.045.0000049
ACGATTT302.1616124E-645.00000419
CGGGATC302.1616124E-645.0000046
ACTAATC302.1616124E-645.00000410
GATCTAC302.1616124E-645.00000410
ACGCGAG207.027258E-445.01
TCACGAC207.027258E-445.025
ATTTATG253.8858096E-545.01
TTTAGTA207.027258E-445.013
GTGTTAG406.7939254E-945.01
CAGGTAG207.027258E-445.01
CAGCGAT502.1827873E-1145.021
AGCCGCA207.027258E-445.032
AGATCGC207.027258E-445.020
CATATGG502.1827873E-1145.02
ACGCCCC207.027258E-445.020
TACTTTC207.027258E-445.025
ATTAGCG207.027258E-445.012
ATTAGAA207.027258E-445.034
Produced by FastQC (version 0.11.5)