##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550211_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	377392
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27144189595964	33.0	31.0	34.0	30.0	34.0
2	32.42200682579387	34.0	31.0	34.0	30.0	34.0
3	32.42163850850044	34.0	31.0	34.0	30.0	34.0
4	35.982127337092464	37.0	35.0	37.0	35.0	37.0
5	35.998778458472884	37.0	35.0	37.0	35.0	37.0
6	35.998314749650234	37.0	35.0	37.0	35.0	37.0
7	36.149126107601646	37.0	35.0	37.0	35.0	37.0
8	35.96162345783694	37.0	35.0	37.0	35.0	37.0
9	37.624523042353836	39.0	38.0	39.0	35.0	39.0
10	37.60186490439649	39.0	37.0	39.0	35.0	39.0
11	37.512684423623185	39.0	37.0	39.0	35.0	39.0
12	37.276722877008524	39.0	37.0	39.0	35.0	39.0
13	37.06840367575359	39.0	37.0	39.0	33.0	39.0
14	38.16917952685802	40.0	37.0	41.0	33.0	41.0
15	38.23260429473863	40.0	37.0	41.0	34.0	41.0
16	38.297141963793614	40.0	37.0	41.0	34.0	41.0
17	38.22293000381566	40.0	37.0	41.0	34.0	41.0
18	38.18913225505575	40.0	36.0	41.0	34.0	41.0
19	38.180138953660915	40.0	36.0	41.0	34.0	41.0
20	38.14620076737186	40.0	36.0	41.0	34.0	41.0
21	38.09954106075381	40.0	36.0	41.0	34.0	41.0
22	38.11097479543817	40.0	36.0	41.0	34.0	41.0
23	38.097548437698734	40.0	36.0	41.0	34.0	41.0
24	38.034974244287106	40.0	36.0	41.0	34.0	41.0
25	37.986804171789544	40.0	36.0	41.0	34.0	41.0
26	37.902684741594946	40.0	35.0	41.0	34.0	41.0
27	37.84303323864841	40.0	36.0	41.0	34.0	41.0
28	37.739260503667275	40.0	36.0	41.0	34.0	41.0
29	37.66352757875101	40.0	36.0	41.0	33.0	41.0
30	37.56995643786832	39.0	36.0	41.0	33.0	41.0
31	37.48350786450163	39.0	35.0	41.0	33.0	41.0
32	37.346077818289736	39.0	35.0	41.0	33.0	41.0
33	37.25449665069742	39.0	35.0	41.0	33.0	41.0
34	37.18877453682113	39.0	35.0	41.0	33.0	41.0
35	37.16326790181032	39.0	35.0	41.0	33.0	41.0
36	37.018892822317376	39.0	35.0	41.0	32.0	41.0
37	36.91146606181371	39.0	35.0	41.0	32.0	41.0
38	36.749088480942895	39.0	35.0	41.0	31.0	41.0
39	36.62204816212321	39.0	35.0	41.0	31.0	41.0
40	36.39194259549752	39.0	35.0	41.0	30.0	41.0
41	36.33334039937254	39.0	35.0	41.0	30.0	41.0
42	36.23219888073939	38.0	35.0	40.0	30.0	41.0
43	36.08256666807988	38.0	35.0	40.0	30.0	41.0
44	35.94111427905202	38.0	35.0	40.0	30.0	41.0
45	35.808021897655486	38.0	35.0	40.0	30.0	41.0
46	35.59720661805232	38.0	35.0	40.0	29.0	41.0
47	35.407894178996905	38.0	35.0	40.0	28.0	41.0
48	35.40666733794039	38.0	35.0	40.0	28.0	41.0
49	35.31761934540213	37.0	35.0	40.0	28.0	41.0
50	35.18410830118285	37.0	34.0	40.0	27.0	41.0
51	33.47692584898461	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	6.0
10	10.0
11	7.0
12	8.0
13	6.0
14	10.0
15	16.0
16	32.0
17	51.0
18	110.0
19	216.0
20	327.0
21	476.0
22	808.0
23	1181.0
24	1666.0
25	2203.0
26	2741.0
27	3112.0
28	3697.0
29	4602.0
30	5601.0
31	7087.0
32	9330.0
33	13204.0
34	26452.0
35	39003.0
36	26808.0
37	41199.0
38	76417.0
39	110961.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.504366812227076	20.83430491372366	23.836488319837198	11.824839954212067
2	30.245209225420783	25.901979904184508	25.396404799253823	18.456406071140883
3	28.84666341628863	25.963719421715354	27.91659600627464	17.27302115572137
4	28.23377284097172	24.69315724763641	28.0156972060881	19.05737270530377
5	24.457858142196972	29.026582439479377	25.74484885742146	20.77071056090219
6	24.286418366049094	35.050822486963156	24.650231059481918	16.012528087505828
7	76.05540128036631	5.793180565565778	11.8089413660067	6.34247678806122
8	74.08185653114003	5.778606859710857	11.346822402170687	8.79271420697842
9	68.81173951753084	5.49985161317675	14.598613643108491	11.089795226183915
10	43.38247763598592	18.894147199728664	21.31285241870522	16.41052274558019
11	30.951901471149363	22.406145334294315	24.060923390002966	22.581029804553356
12	26.989443337431634	17.779656166532412	32.71373002077416	22.517170475261796
13	22.70609869843558	23.683331920125493	35.164762369101624	18.445807012337305
14	18.158837495230422	28.198530970449827	31.498812905414	22.143818628905755
15	16.443379827871286	22.68967015729003	40.90070801712808	19.966241997710604
16	17.912674354517318	24.06436808411413	32.990895408487724	25.032062152880822
17	17.978653495569592	24.261245601390595	28.72212447534659	29.03797642769322
18	20.782369525586127	24.255946071988806	31.466485776063085	23.49519862636198
19	22.08128418196464	25.641773010556662	27.54350913638869	24.73343367109001
20	22.463910204773814	26.84238139653199	29.323621062449657	21.37008733624454
21	21.205271971848898	27.433278924831473	30.797685165557297	20.563763937762328
22	18.92514944672913	24.397708483486667	27.51277186585831	29.164370203925895
23	18.13207487175139	26.411264679696444	28.42879552295756	27.027864925594606
24	22.645154110315005	21.84757493534574	29.414507991690336	26.09276296264892
25	20.63954720820791	26.29997456225887	26.828072667147158	26.232405562386056
26	18.308019247890787	27.43115911307076	27.977805570865304	26.283016068173147
27	21.62790944164158	26.103097044982405	29.095741298172722	23.17325221520329
28	17.840335778182897	26.274006868190103	31.155138423707974	24.730518929919025
29	23.17828676813499	26.37310806800356	26.851390596515028	23.59721456734642
30	23.150729215245686	28.680523169542543	26.97831432568788	21.19043328952389
31	24.58531182431	31.62388179929622	20.81973120786874	22.971075168525033
32	25.157396023233137	30.322052401746724	21.10882053673634	23.4117310382838
33	21.802263958960445	29.269830839021495	22.215627252299996	26.712277949718064
34	22.197608852333914	27.551723406961457	25.328040869970746	24.92262687073388
35	20.21161020901344	25.02490778818841	25.58533302242761	29.178148980370544
36	21.817102641285455	28.270074617373975	26.01247509221181	23.900347649128758
37	23.441938355873997	25.499215669648535	28.60977445202866	22.449071522448808
38	21.79934921778946	29.641062873616825	22.29273540509603	26.266852503497688
39	27.046678254970956	24.261245601390595	24.77159028278289	23.920485860855557
40	24.65394073006317	22.368518675541612	27.682886759655744	25.294653834739478
41	20.882795607750033	23.584760673252212	23.78163819052868	31.750805528469073
42	21.16102090134396	22.912515368635265	27.473290371814983	28.45317335820579
43	23.6178827320134	21.519534065374994	27.9579323356086	26.90465086700301
44	21.088947301479628	23.4997032263535	27.019915631491926	28.391433840674946
45	22.843356509941916	22.70026921609361	26.53394878534786	27.92242548861661
46	26.324617373977187	24.341268495357614	26.350320091575867	22.98379403908933
47	19.902382668419044	23.39318268537754	31.76166956374274	24.942765082460678
48	20.397888667486328	21.248198160003394	28.787308686988595	29.566604485521687
49	22.08128418196464	18.388042141857802	32.39231356255565	27.13836011362191
50	21.785305464874718	18.923824564378684	30.782581506762202	28.5082884639844
51	20.803832619663375	19.55340865731123	25.489411540255226	34.15334718277017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	351.0
1	465.0
2	579.0
3	892.5
4	1206.0
5	895.0
6	584.0
7	558.5
8	533.0
9	605.5
10	678.0
11	726.0
12	774.0
13	880.0
14	986.0
15	963.5
16	941.0
17	877.5
18	814.0
19	891.0
20	968.0
21	1003.5
22	1039.0
23	1085.0
24	1131.0
25	998.0
26	1055.0
27	1245.0
28	1377.5
29	1510.0
30	2323.5
31	3137.0
32	3154.5
33	3172.0
34	3555.0
35	3938.0
36	4674.0
37	5410.0
38	6396.0
39	7382.0
40	9786.5
41	12191.0
42	16006.5
43	19822.0
44	22882.0
45	25942.0
46	36419.0
47	46896.0
48	51717.5
49	56539.0
50	55021.0
51	53503.0
52	43172.5
53	32842.0
54	26233.0
55	19624.0
56	17487.0
57	15350.0
58	14261.0
59	13172.0
60	11870.5
61	10569.0
62	10511.5
63	10454.0
64	9129.5
65	7805.0
66	6566.0
67	5327.0
68	4440.5
69	3554.0
70	3108.0
71	2662.0
72	2228.5
73	1795.0
74	1396.5
75	732.0
76	466.0
77	378.0
78	290.0
79	245.0
80	200.0
81	130.0
82	60.0
83	51.0
84	42.0
85	30.0
86	18.0
87	14.5
88	11.0
89	9.5
90	8.0
91	7.5
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	377392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.670007716534183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.61409218562333	17.87872429373049
2	14.299009798427504	7.913074229225738
3	6.299262574297823	5.229019321178872
4	3.336123858390157	3.6924229161987774
5	2.0045494731540963	2.773294969517419
6	1.283448551425221	2.1307818793026287
7	1.0016490574221526	1.9400946003691109
8	0.6929876848956172	1.5339979670819912
9	0.5296972120996591	1.3191053351601791
>10	5.1077940279488745	33.00325172687238
>50	0.7288314273807888	12.943650894029524
>100	0.09060609274815816	4.690151820270712
>500	0.004978356744404295	0.8548837581238262
>1k	0.006969699442166012	4.097546288938339
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	3632	0.9623945393649044	No Hit
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	2463	0.6526370458303302	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC	2087	0.5530058930766948	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG	1935	0.5127294696230975	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	1891	0.5010705049391614	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	1785	0.472982999109679	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	1080	0.2861745876966125	No Hit
ACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	834	0.22099037605460636	TruSeq Adapter, Index 15 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	594	0.1573960232331369	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	580	0.15368635265188452	No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT	570	0.15103658795098995	No Hit
CGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	525	0.13911264679696442	TruSeq Adapter, Index 15 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	491	0.1301034468139229	No Hit
AGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	460	0.12188917624114978	TruSeq Adapter, Index 15 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	459	0.12162419977106032	TruSeq Adapter, Index 15 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	439	0.11632467036927122	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	405	0.1073154703862297	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6497647008945607E-4	0.0	0.0	0.536312375461059	0.0
2	2.6497647008945607E-4	0.0	0.0	2.982840123797007	0.0
3	2.6497647008945607E-4	0.0	0.0	4.128333403993725	0.0
4	2.6497647008945607E-4	0.0	0.0	5.474943824988341	0.0
5	2.6497647008945607E-4	0.0	0.0	9.271261711959978	0.0
6	2.6497647008945607E-4	0.0	0.0	10.920740238266841	0.0
7	2.6497647008945607E-4	0.0	0.0	12.800483317081444	0.0
8	2.6497647008945607E-4	0.0	0.0	15.757355746809683	0.0
9	2.6497647008945607E-4	0.0	0.0	16.839784627125113	0.0
10	2.6497647008945607E-4	0.0	0.0	19.725643362869377	0.0
11	2.6497647008945607E-4	0.0	0.0	22.750879721880697	0.0
12	2.6497647008945607E-4	0.0	0.0	25.60467630474414	0.0
13	2.6497647008945607E-4	0.0	0.0	26.555411879425108	0.0
14	2.6497647008945607E-4	0.0	0.0	26.883982702336034	0.0
15	2.6497647008945607E-4	0.0	0.0	27.550133548140924	0.0
16	2.6497647008945607E-4	0.0	0.0	28.850108110399795	0.0
17	2.6497647008945607E-4	0.0	0.0	30.3829969898673	0.0
18	2.6497647008945607E-4	0.0	0.0	32.30513630389621	0.0
19	2.6497647008945607E-4	0.0	0.0	33.324500784330354	0.0
20	2.6497647008945607E-4	0.0	0.0	34.32823165302921	0.0
21	2.6497647008945607E-4	0.0	0.0	35.52778013312418	0.0
22	2.6497647008945607E-4	0.0	0.0	36.58450629584093	0.0
23	2.6497647008945607E-4	0.0	0.0	37.65130156442108	0.0
24	2.6497647008945607E-4	0.0	0.0	38.485447492262686	0.0
25	2.6497647008945607E-4	0.0	0.0	39.133314961631406	0.0
26	2.6497647008945607E-4	0.0	0.0	39.77800271335905	0.0
27	2.6497647008945607E-4	0.0	0.0	40.38373892398355	0.0
28	2.6497647008945607E-4	0.0	0.0	40.97172171111205	0.0
29	2.6497647008945607E-4	0.0	0.0	41.6087251452071	0.0
30	2.6497647008945607E-4	0.0	0.0	42.348539449696865	0.0
31	2.6497647008945607E-4	0.0	0.0	43.016280154322295	0.0
32	2.6497647008945607E-4	0.0	0.0	43.61804171789545	0.0
33	2.6497647008945607E-4	0.0	0.0	44.2425912578963	0.0
34	2.6497647008945607E-4	0.0	0.0	44.838523339127484	0.0
35	2.6497647008945607E-4	0.0	0.0	45.44425954975198	0.0
36	2.6497647008945607E-4	0.0	0.0	46.02058337219655	0.0
37	2.6497647008945607E-4	0.0	0.0	46.57729893585449	0.0
38	2.6497647008945607E-4	0.0	0.0	47.141963793615126	0.0
39	2.6497647008945607E-4	0.0	0.0	47.730476533683806	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGTT	30	2.1616124E-6	45.000004	10
TACCAAT	120	0.0	45.000004	9
ACGATTT	30	2.1616124E-6	45.000004	19
CGGGATC	30	2.1616124E-6	45.000004	6
ACTAATC	30	2.1616124E-6	45.000004	10
GATCTAC	30	2.1616124E-6	45.000004	10
ACGCGAG	20	7.027258E-4	45.0	1
TCACGAC	20	7.027258E-4	45.0	25
ATTTATG	25	3.8858096E-5	45.0	1
TTTAGTA	20	7.027258E-4	45.0	13
GTGTTAG	40	6.7939254E-9	45.0	1
CAGGTAG	20	7.027258E-4	45.0	1
CAGCGAT	50	2.1827873E-11	45.0	21
AGCCGCA	20	7.027258E-4	45.0	32
AGATCGC	20	7.027258E-4	45.0	20
CATATGG	50	2.1827873E-11	45.0	2
ACGCCCC	20	7.027258E-4	45.0	20
TACTTTC	20	7.027258E-4	45.0	25
ATTAGCG	20	7.027258E-4	45.0	12
ATTAGAA	20	7.027258E-4	45.0	34
>>END_MODULE