FastQCFastQC Report
Sat 18 Jun 2016
SRR3550210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419150
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC93090.6559560300179684TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC80670.5684388542437375TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT57210.40312863333685656TruSeq Adapter, Index 27 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC26660.18785892964098228TruSeq Adapter, Index 16 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC23330.16439417961455802TruSeq Adapter, Index 27 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG19430.13691294084487193TruSeq Adapter, Index 16 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG16020.11288447310009514TruSeq Adapter, Index 27 (95% over 22bp)
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG15670.1104182080823028No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG15320.10795194306451046TruSeq Adapter, Index 27 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG14320.10090547158510377TruSeq Adapter, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT502.1827873E-1145.00000427
GCTATCG253.8912694E-545.00000435
ATTCGTC253.8912694E-545.00000440
GCTTCGT302.1658634E-645.00000422
CCGAACA302.1658634E-645.00000413
TTCGAAT253.8912694E-545.00000417
TCGAATA253.8912694E-545.00000418
GCGTACG253.8912694E-545.0000041
CGAACCG207.033848E-445.02
CCGATTT207.033848E-445.012
TTTACGC207.033848E-445.015
ATTACGA453.8562575E-1045.037
CCAATCG207.033848E-445.01
TATTCGA207.033848E-445.017
GTCCGAT207.033848E-445.016
CATACGT207.033848E-445.014
TCCCGAA207.033848E-445.013
ACGTCGG453.8562575E-1045.02
ATACGTC207.033848E-445.015
TTGCACG453.8562575E-1045.01
Produced by FastQC (version 0.11.5)