##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1419150
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25629214670754	33.0	31.0	34.0	30.0	34.0
2	32.42434696825565	34.0	31.0	34.0	30.0	34.0
3	32.445704823309725	34.0	31.0	34.0	30.0	34.0
4	35.97564739456717	37.0	35.0	37.0	35.0	37.0
5	35.99666772363739	37.0	35.0	37.0	35.0	37.0
6	36.017123630342105	37.0	35.0	37.0	35.0	37.0
7	36.13994503752246	37.0	35.0	37.0	35.0	37.0
8	36.12233872388401	37.0	35.0	37.0	35.0	37.0
9	37.71358912024804	39.0	38.0	39.0	35.0	39.0
10	37.53344396293556	39.0	37.0	39.0	35.0	39.0
11	37.35479547616531	39.0	37.0	39.0	35.0	39.0
12	37.078895817919175	39.0	37.0	39.0	34.0	39.0
13	36.955108339499	39.0	35.0	39.0	33.0	39.0
14	38.0425677342071	40.0	37.0	41.0	33.0	41.0
15	38.09017017228623	40.0	37.0	41.0	33.0	41.0
16	38.181942007539725	40.0	37.0	41.0	34.0	41.0
17	38.14990592960575	40.0	37.0	41.0	34.0	41.0
18	38.1023105379981	40.0	36.0	41.0	34.0	41.0
19	38.063112426452456	40.0	36.0	41.0	34.0	41.0
20	37.96112250290667	40.0	36.0	41.0	34.0	41.0
21	37.880216326674415	40.0	36.0	41.0	33.0	41.0
22	37.79477856463376	40.0	35.0	41.0	33.0	41.0
23	37.74785963428813	39.0	35.0	41.0	33.0	41.0
24	37.75779304513265	40.0	35.0	41.0	33.0	41.0
25	37.61552337666913	39.0	35.0	41.0	33.0	41.0
26	37.47461860973118	39.0	35.0	41.0	33.0	41.0
27	37.385372934503046	39.0	35.0	41.0	33.0	41.0
28	37.26253743437974	39.0	35.0	41.0	32.0	41.0
29	37.26022548708734	39.0	35.0	41.0	32.0	41.0
30	37.16580770179333	39.0	35.0	41.0	32.0	41.0
31	37.03014691893035	39.0	35.0	41.0	32.0	41.0
32	36.957222985589965	39.0	35.0	41.0	31.0	41.0
33	36.85076348518479	39.0	35.0	41.0	31.0	41.0
34	36.756583870626784	39.0	35.0	41.0	31.0	41.0
35	36.72133037381531	39.0	35.0	41.0	31.0	41.0
36	36.70204911390621	39.0	35.0	41.0	31.0	41.0
37	36.655114681323326	39.0	35.0	41.0	31.0	41.0
38	36.594211323679666	39.0	35.0	41.0	31.0	41.0
39	36.56728816545115	39.0	35.0	41.0	31.0	41.0
40	36.45401895500828	39.0	35.0	40.0	30.0	41.0
41	36.33748722827044	38.0	35.0	40.0	30.0	41.0
42	36.30195328189409	38.0	35.0	40.0	30.0	41.0
43	36.22302223161752	38.0	35.0	40.0	30.0	41.0
44	36.1247373427756	38.0	35.0	40.0	30.0	41.0
45	36.00777507663038	38.0	35.0	40.0	30.0	41.0
46	35.943786773773034	38.0	35.0	40.0	30.0	41.0
47	35.90905330655674	38.0	35.0	40.0	30.0	41.0
48	35.873415777049644	38.0	35.0	40.0	30.0	41.0
49	35.87713772328507	38.0	35.0	40.0	30.0	41.0
50	35.73962935560019	38.0	35.0	40.0	29.0	41.0
51	34.351786632843606	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	15.0
9	54.0
10	72.0
11	53.0
12	29.0
13	29.0
14	38.0
15	47.0
16	94.0
17	173.0
18	307.0
19	554.0
20	875.0
21	1477.0
22	2349.0
23	3446.0
24	5281.0
25	7397.0
26	9866.0
27	12373.0
28	15295.0
29	18768.0
30	23498.0
31	29604.0
32	39202.0
33	56807.0
34	108782.0
35	133960.0
36	105351.0
37	163887.0
38	299344.0
39	379907.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.619032519465875	21.61850403410492	22.879470105344748	10.882993341084452
2	31.70996723390762	23.745622379593417	23.966670189902405	20.577740196596555
3	32.759680090194834	23.71652045238347	25.763590881865905	17.76020857555579
4	29.963640207166257	25.45213684247613	24.707888524821193	19.876334425536413
5	25.60758200331184	29.660501004122185	24.19941514286721	20.532501849698765
6	27.599478561110523	32.64919141739774	23.86675122432442	15.884578797167318
7	78.6257971320861	6.355987739139627	9.326075467709545	5.692139661064722
8	78.01867314942042	5.563752950709932	9.809674805341226	6.607899094528415
9	72.3919247436846	7.056125145333474	12.441250044040448	8.11070006694148
10	45.230807173307966	20.585843638797872	19.51104534404397	14.672303843850193
11	31.89134340978755	24.87552408131628	23.172603318888065	20.060529190008104
12	28.826551104534403	22.830919916851634	28.727407250819155	19.615121727794808
13	25.192333439030406	24.27960398830286	29.804812740020438	20.7232498326463
14	20.38325758376493	26.543071556917873	30.333439030405522	22.74023182891167
15	19.811718282070252	26.488038614663704	32.990945284148964	20.70929781911708
16	23.741253567276186	26.650036993975267	28.250642990522497	21.35806644822605
17	22.865236232956345	26.964802874960363	27.12412359510975	23.04583729697354
18	24.269104745798543	26.08420533417891	29.542895395130884	20.10379452489166
19	22.981009759363	28.13606736426734	26.696825564598527	22.18609731177113
20	25.635838353944262	28.245992319346087	27.061339534228235	19.056829792481413
21	25.149843216009582	27.49455660078216	28.312863333685655	19.0427368495226
22	23.796145580100763	24.465137582355638	28.00697600676461	23.73174083077899
23	22.888630518267977	25.691646408061164	28.91935313391819	22.50036993975267
24	25.46869605045274	23.07754641863087	28.925060775816508	22.528696755099883
25	21.42099143853715	26.169749497938906	27.158369446499663	25.250889617024274
26	20.315470528133037	27.000739879505335	29.021104182080826	23.6626854102808
27	21.18775323256879	25.936370362540956	28.164182785470178	24.711693619420075
28	17.782334496001127	26.61114047140894	30.230419617376597	25.376105415213328
29	19.941796145580103	24.39622309128704	30.189127294507273	25.472853468625583
30	21.31141880703238	24.707254342388048	29.816932670965013	24.164394179614558
31	22.468942676954516	24.693654652432794	28.132050875524083	24.70535179508861
32	22.484303984779622	24.484726773068385	28.273262163971392	24.7577070781806
33	22.19427121868724	22.912729450727547	28.136419687841315	26.756579642743894
34	19.35524785963429	22.767853997110947	31.797132086107883	26.079766057146887
35	18.65616742416235	23.977874079554663	30.98587182468379	26.380086671599194
36	20.291019272099497	25.60638410316034	28.834513617306136	25.26808300743403
37	20.335059718845788	25.338547722228093	29.627664447028152	24.698728111897967
38	19.973434802522636	26.484656308353593	27.819258006553216	25.72265088257055
39	21.49702286579995	23.470034880033825	28.654969524010852	26.377972730155374
40	22.60190959377092	21.840538350421028	29.838424408977204	25.71912764683085
41	18.605291900081035	23.914455836240002	28.326181164781733	29.154071098897226
42	20.099918965577988	21.85526547581299	29.10143395694606	28.943381601662963
43	21.335940527780714	21.632596977063738	29.17126448930698	27.86019800584857
44	19.153718775323256	22.455977169432405	29.11031251101011	29.279991544234225
45	20.904555543811437	21.997815593841384	28.54596061022443	28.55166825212275
46	21.819680794841982	22.405947221928617	28.98629461297255	26.788077370256847
47	18.126202304196173	22.2458513899165	32.60705351795089	27.02089278793644
48	19.281471303244903	20.977486523623295	32.19272099496177	27.54832117817003
49	21.326991509001868	19.827854701758092	32.99749850262481	25.84765528661523
50	19.934749674100694	20.73980904062291	31.83574674981503	27.489694535461368
51	18.898425113624352	20.21625620970299	29.620336116689565	31.26498255998309
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	637.0
1	1228.5
2	1820.0
3	2451.5
4	3083.0
5	2450.0
6	1817.0
7	1769.5
8	1722.0
9	1824.5
10	1927.0
11	1987.5
12	2048.0
13	2124.5
14	2201.0
15	2308.5
16	2416.0
17	2256.5
18	2097.0
19	2489.5
20	2882.0
21	3345.5
22	3809.0
23	4103.5
24	4398.0
25	5491.5
26	7727.5
27	8870.0
28	10852.0
29	12834.0
30	15303.5
31	17773.0
32	21138.0
33	24503.0
34	27396.5
35	30290.0
36	33382.5
37	36475.0
38	39696.0
39	42917.0
40	48926.5
41	54936.0
42	64863.5
43	74791.0
44	85104.5
45	95418.0
46	110529.0
47	125640.0
48	144049.0
49	162458.0
50	164556.5
51	166655.0
52	140111.0
53	113567.0
54	101684.0
55	89801.0
56	78378.5
57	66956.0
58	63820.0
59	60684.0
60	56936.5
61	53189.0
62	49637.0
63	46085.0
64	42126.5
65	38168.0
66	30145.5
67	22123.0
68	17760.5
69	13398.0
70	11259.5
71	9121.0
72	7901.0
73	6681.0
74	5572.0
75	3264.5
76	2066.0
77	1463.0
78	860.0
79	612.5
80	365.0
81	340.5
82	316.0
83	245.5
84	175.0
85	133.5
86	92.0
87	57.5
88	23.0
89	15.5
90	8.0
91	6.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1419150.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.279217981561775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.4855248732722	13.934807584153447
2	13.054686414491098	5.555870357897785
3	6.014003632375579	3.8391988270567285
4	3.431347873968553	2.920655975229816
5	2.1105899606152905	2.2455851920814327
6	1.4435492026780128	1.8430558890537085
7	1.0000771444273486	1.4896601688253175
8	0.7465165064886148	1.270822997472417
9	0.577789434901473	1.106541659944066
>10	3.8677453463618283	17.83067081096683
>50	1.5216419900233384	23.784528473572504
>100	0.7348942870426763	20.44161388865225
>500	0.007644761918405507	1.0036312780032581
>1k	0.0029914285767673725	1.0997507217528304
>5k	9.971428589224574E-4	1.6336061753376503
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	9309	0.6559560300179684	TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	8067	0.5684388542437375	TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	5721	0.40312863333685656	TruSeq Adapter, Index 27 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2666	0.18785892964098228	TruSeq Adapter, Index 16 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2333	0.16439417961455802	TruSeq Adapter, Index 27 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1943	0.13691294084487193	TruSeq Adapter, Index 16 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1602	0.11288447310009514	TruSeq Adapter, Index 27 (95% over 22bp)
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	1567	0.1104182080823028	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1532	0.10795194306451046	TruSeq Adapter, Index 27 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1432	0.10090547158510377	TruSeq Adapter, Index 16 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4092942958813373E-4	0.0	0.0	0.4370926258675968	0.0
2	1.4092942958813373E-4	0.0	0.0	2.1680583447838493	0.0
3	1.4092942958813373E-4	0.0	0.0	2.999894302927809	0.0
4	1.4092942958813373E-4	0.0	0.0	3.818835218264454	0.0
5	1.4092942958813373E-4	0.0	0.0	5.955395835535356	0.0
6	1.4092942958813373E-4	0.0	0.0	6.963464045379276	0.0
7	1.4092942958813373E-4	0.0	0.0	8.046999964767643	0.0
8	1.4092942958813373E-4	0.0	0.0	9.6092731564669	0.0
9	1.4092942958813373E-4	0.0	0.0	10.136208293696932	0.0
10	1.4092942958813373E-4	0.0	0.0	11.379417256808653	0.0
11	1.4092942958813373E-4	0.0	0.0	13.37554169749498	0.0
12	1.4092942958813373E-4	0.0	0.0	14.87185991614699	0.0
13	1.4092942958813373E-4	0.0	0.0	15.512806961913821	0.0
14	1.4092942958813373E-4	0.0	0.0	15.78480076101892	0.0
15	1.4092942958813373E-4	0.0	0.0	16.333439030405525	0.0
16	1.4092942958813373E-4	0.0	0.0	17.345100940703944	0.0
17	1.4092942958813373E-4	0.0	0.0	18.47422753056407	0.0
18	1.4092942958813373E-4	0.0	0.0	19.583412606137475	0.0
19	1.4092942958813373E-4	0.0	0.0	20.41130254025297	0.0
20	1.4092942958813373E-4	0.0	0.0	21.18430046154388	0.0
21	1.4092942958813373E-4	0.0	0.0	22.1543881901138	0.0
22	1.4092942958813373E-4	0.0	0.0	23.07162738258817	0.0
23	2.1139414438220062E-4	0.0	0.0	23.95617094739809	0.0
24	2.8185885917626747E-4	0.0	0.0	24.687665151675297	0.0
25	2.8185885917626747E-4	0.0	0.0	25.36285804883205	0.0
26	2.8185885917626747E-4	0.0	0.0	25.94489659303104	0.0
27	4.2278828876440123E-4	0.0	0.0	26.507768734806046	0.0
28	4.2278828876440123E-4	0.0	0.0	27.067258570270937	0.0
29	4.2278828876440123E-4	0.0	0.0	27.672057217348414	0.0
30	4.2278828876440123E-4	0.0	0.0	28.355846809710037	0.0
31	4.2278828876440123E-4	0.0	0.0	28.9667758869746	0.0
32	4.2278828876440123E-4	0.0	0.0	29.554944861360674	0.0
33	4.2278828876440123E-4	0.0	0.0	30.164887432618116	0.0
34	4.932530035584681E-4	0.0	0.0	30.75369058943734	0.0
35	4.932530035584681E-4	0.0	0.0	31.367508720008455	0.0
36	4.932530035584681E-4	0.0	0.0	31.924884614029526	0.0
37	5.637177183525349E-4	0.0	0.0	32.519606806891446	0.0
38	5.637177183525349E-4	0.0	0.0	33.13462283761407	0.0
39	5.637177183525349E-4	0.0	0.0	33.797413944967055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTT	50	2.1827873E-11	45.000004	27
GCTATCG	25	3.8912694E-5	45.000004	35
ATTCGTC	25	3.8912694E-5	45.000004	40
GCTTCGT	30	2.1658634E-6	45.000004	22
CCGAACA	30	2.1658634E-6	45.000004	13
TTCGAAT	25	3.8912694E-5	45.000004	17
TCGAATA	25	3.8912694E-5	45.000004	18
GCGTACG	25	3.8912694E-5	45.000004	1
CGAACCG	20	7.033848E-4	45.0	2
CCGATTT	20	7.033848E-4	45.0	12
TTTACGC	20	7.033848E-4	45.0	15
ATTACGA	45	3.8562575E-10	45.0	37
CCAATCG	20	7.033848E-4	45.0	1
TATTCGA	20	7.033848E-4	45.0	17
GTCCGAT	20	7.033848E-4	45.0	16
CATACGT	20	7.033848E-4	45.0	14
TCCCGAA	20	7.033848E-4	45.0	13
ACGTCGG	45	3.8562575E-10	45.0	2
ATACGTC	20	7.033848E-4	45.0	15
TTGCACG	45	3.8562575E-10	45.0	1
>>END_MODULE