FastQCFastQC Report
Sat 18 Jun 2016
SRR3550208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824207
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC23520.28536520558549006No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC17500.21232530177491818No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.1703455563954201No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT13560.16452177668959375Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCA207.0321246E-445.01
ACGATAG302.164752E-644.9999961
CGTTAGG1000.040.52
ACGGGCC1050.038.571435
ACGCACC356.2461404E-638.57142638
GCCGATA356.2461404E-638.5714269
TTAAGCG356.2461404E-638.5714261
GACCTAT1250.037.833
CGAAACG301.13964205E-437.49999642
TAGCCGT301.13964205E-437.49999644
TTACGGC301.13964205E-437.49999640
TACGAAT1450.037.2413812
TATGCGG850.037.0588232
CGTGCGG1350.036.6666682
TAGTTAG1050.036.428571
CTCACGA250.002106763736.024
TCCGAAC250.002106763736.036
GTATGCG250.002106763736.01
CTACGAC250.002106763736.024
GTACTAC504.876165E-836.030
Produced by FastQC (version 0.11.5)