##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550208_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	824207
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.282455742307455	33.0	31.0	34.0	30.0	34.0
2	32.45400366655464	34.0	31.0	34.0	31.0	34.0
3	32.44226389729764	34.0	31.0	34.0	30.0	34.0
4	35.9903191795265	37.0	35.0	37.0	35.0	37.0
5	36.000832315182954	37.0	35.0	37.0	35.0	37.0
6	36.016420632195555	37.0	35.0	37.0	35.0	37.0
7	36.12694747800006	37.0	35.0	37.0	35.0	37.0
8	36.14659666807004	37.0	35.0	37.0	35.0	37.0
9	37.62254021137894	39.0	38.0	39.0	35.0	39.0
10	37.468572822118716	39.0	37.0	39.0	35.0	39.0
11	37.33459676998618	39.0	37.0	39.0	35.0	39.0
12	37.16081154370201	39.0	37.0	39.0	34.0	39.0
13	37.08764303142293	39.0	37.0	39.0	33.0	39.0
14	38.252099290590834	40.0	37.0	41.0	33.0	41.0
15	38.3339549409311	40.0	38.0	41.0	34.0	41.0
16	38.41266696351766	40.0	38.0	41.0	34.0	41.0
17	38.38056944432648	40.0	37.0	41.0	34.0	41.0
18	38.299075353642955	40.0	37.0	41.0	34.0	41.0
19	38.252761745532375	40.0	37.0	41.0	34.0	41.0
20	38.138832841749704	40.0	37.0	41.0	34.0	41.0
21	38.052856867267565	40.0	37.0	41.0	34.0	41.0
22	38.01967588239362	40.0	36.0	41.0	34.0	41.0
23	38.026879169917265	40.0	36.0	41.0	34.0	41.0
24	38.038347162787986	40.0	36.0	41.0	34.0	41.0
25	37.97201916508838	40.0	36.0	41.0	34.0	41.0
26	37.8992704502631	40.0	36.0	41.0	33.0	41.0
27	37.85135894259573	40.0	36.0	41.0	33.0	41.0
28	37.764792097130936	40.0	36.0	41.0	33.0	41.0
29	37.7309874825135	40.0	36.0	41.0	33.0	41.0
30	37.63333846958349	40.0	36.0	41.0	33.0	41.0
31	37.570509592857135	40.0	36.0	41.0	33.0	41.0
32	37.465526257360104	40.0	36.0	41.0	33.0	41.0
33	37.35097979027113	40.0	35.0	41.0	33.0	41.0
34	37.21941211370444	40.0	35.0	41.0	32.0	41.0
35	37.15630418086719	39.0	35.0	41.0	32.0	41.0
36	37.05119709005141	39.0	35.0	41.0	31.0	41.0
37	36.987413356110785	39.0	35.0	41.0	31.0	41.0
38	36.890589378639106	39.0	35.0	41.0	31.0	41.0
39	36.807898986541005	39.0	35.0	41.0	31.0	41.0
40	36.69316567318647	39.0	35.0	41.0	31.0	41.0
41	36.58777952625979	39.0	35.0	41.0	31.0	41.0
42	36.53870204936381	39.0	35.0	41.0	31.0	41.0
43	36.4324593215054	39.0	35.0	41.0	30.0	41.0
44	36.33719199181759	39.0	35.0	41.0	30.0	41.0
45	36.2007978578197	38.0	35.0	40.0	30.0	41.0
46	36.134099807451285	38.0	35.0	40.0	30.0	41.0
47	36.09146367356744	38.0	35.0	40.0	30.0	41.0
48	36.03092791010025	38.0	35.0	40.0	30.0	41.0
49	35.942624850310665	38.0	35.0	40.0	29.0	41.0
50	35.81508407475307	38.0	35.0	40.0	29.0	41.0
51	34.4655104846234	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	11.0
9	24.0
10	34.0
11	20.0
12	12.0
13	17.0
14	17.0
15	21.0
16	44.0
17	90.0
18	176.0
19	316.0
20	562.0
21	877.0
22	1391.0
23	2157.0
24	3125.0
25	4559.0
26	6103.0
27	7386.0
28	8426.0
29	10012.0
30	12688.0
31	15937.0
32	20767.0
33	29205.0
34	53488.0
35	64586.0
36	60439.0
37	90868.0
38	163844.0
39	266907.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.812311712955605	21.731069986059328	24.64368781143572	11.812930489549348
2	32.456894930521095	24.530488093403722	24.641018579070547	18.371598397004636
3	32.870383289634766	23.554641006446197	26.543817269205427	17.031158434713607
4	29.982273870520395	25.35467425052202	25.354916908009763	19.308134970947833
5	26.297641247890397	30.325755544420275	23.885504490983458	19.491098716705878
6	27.637110580230452	34.072508483912415	23.520911615649954	14.769469320207183
7	80.99166835515835	6.48259478504793	7.879695270726892	4.646041589066825
8	82.42286221786517	5.3166255564439515	7.707529783173402	4.552982442517474
9	75.1731057853185	7.820486843717658	10.353588358264368	6.652819012699479
10	42.801140975507366	24.990202703932386	17.975217390776834	14.233438929783414
11	30.951933191540476	26.971501091352053	23.817803051903226	18.25876266520425
12	29.580433070818373	23.892784215615738	27.756012749224407	18.770769964341483
13	25.465083407444972	24.226316932518166	28.745205998007783	21.56339366202908
14	20.396939118449612	27.864359317501552	28.73841158835099	23.000289975697854
15	20.40191359694834	27.535437092866232	31.806208877138875	20.256440433046553
16	23.511569302371857	26.91593252665896	29.704188389567182	19.868309781402
17	23.10002220316013	27.208820114364475	27.989813238664556	21.70134444381084
18	23.36027235876424	28.005828632855582	28.410096007435026	20.22380300094515
19	24.573074482502573	28.834746610984862	26.007786878781662	20.584392027730896
20	26.96858920149914	27.045754282601337	27.539926256389474	18.44573025951005
21	26.390579065695874	27.561644101542456	27.039202530432284	19.00857430232939
22	24.369363521542525	25.945666561919516	27.43570486540396	22.249265051134
23	23.982203499848946	26.542603981766717	27.999519538174265	21.47567298021007
24	23.904189117539648	25.560933115103367	28.815091354477694	21.719786412879287
25	22.76721745872093	27.11090781806027	26.88717761436144	23.234697108857365
26	21.922284086400627	29.208317813364847	27.422844018553594	21.446554081680937
27	23.38126223145399	28.660518534785556	27.451962917082724	20.506256316677728
28	21.37399949284585	28.969421516682097	28.07425804439904	21.582320946073015
29	23.159594616400977	27.066986812778826	28.36496171471487	21.408456856105325
30	23.60511376389669	26.745222984031926	28.236959889930564	21.41270336214082
31	23.625011677891596	28.10034372433139	26.31450594328852	21.960138654488496
32	25.577676481757617	28.170593067032918	25.8020133291758	20.449717122033665
33	24.544319570205058	28.69157869321663	25.856489935174054	20.907611801404258
34	22.48039630820898	27.75018896951858	28.14899655062381	21.620418171648627
35	24.362447783141857	25.87602386293735	27.497582524778363	22.263945829142436
36	23.14151663356414	29.551920816008597	26.526467258831822	20.780095291595437
37	23.554398348958454	29.027052670021003	26.819112189049594	20.59943679197095
38	23.502469646581503	28.46991107816362	26.3285800775776	21.699039197677283
39	23.68482674862019	26.956456327112	27.637959881437553	21.72075704283026
40	23.998825537759323	25.520530643394196	28.779299375035638	21.70134444381084
41	21.678413311219145	27.238788314100702	27.894691503469392	23.18810687121075
42	23.015213411194033	27.5627360602373	26.720714577769904	22.701335950798768
43	23.917292621877756	26.861819906892322	26.833792967058034	22.38709450417189
44	22.133153443249086	27.30479115076674	27.228596699615508	23.333458706368667
45	22.715410085087846	25.81936333954941	27.009234330696053	24.455992244666692
46	22.385031915526074	27.69607634975194	27.04539029636972	22.87350143835226
47	22.125145746153578	26.599385833898527	28.92865505874131	22.346813361206593
48	22.02286561507	25.65714680899337	29.48531133562321	22.834676240313417
49	22.41111759545842	26.05389180145279	28.855251168699127	22.679739434389663
50	20.977011842898687	25.67194891574568	28.85549382618687	24.495545415168763
51	20.77584878555994	25.37348020582208	27.83730300761823	26.013368000999748
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	256.0
1	728.0
2	1200.0
3	2207.5
4	3215.0
5	2509.5
6	1804.0
7	1690.5
8	1577.0
9	1557.0
10	1537.0
11	1521.0
12	1505.0
13	1550.0
14	1595.0
15	1618.5
16	1642.0
17	1675.5
18	1709.0
19	1799.5
20	1890.0
21	2361.0
22	2832.0
23	3138.0
24	3444.0
25	4328.0
26	6680.5
27	8149.0
28	10082.0
29	12015.0
30	14067.5
31	16120.0
32	17511.0
33	18902.0
34	21825.0
35	24748.0
36	27102.5
37	29457.0
38	31558.5
39	33660.0
40	36416.0
41	39172.0
42	43357.5
43	47543.0
44	50182.5
45	52822.0
46	58816.0
47	64810.0
48	69082.5
49	73355.0
50	72929.5
51	72504.0
52	65102.5
53	57701.0
54	51820.0
55	45939.0
56	42073.5
57	38208.0
58	37462.0
59	36716.0
60	36093.0
61	35470.0
62	32251.0
63	29032.0
64	25115.0
65	21198.0
66	17343.0
67	13488.0
68	11567.5
69	9647.0
70	7612.5
71	5578.0
72	4750.5
73	3923.0
74	3037.0
75	1660.5
76	1170.0
77	971.5
78	773.0
79	550.0
80	327.0
81	228.5
82	130.0
83	87.0
84	44.0
85	32.0
86	20.0
87	14.5
88	9.0
89	7.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	824207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.453751291635697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.67546911689881	20.678897232551897
2	9.943053006296672	5.658343146414325
3	3.241144698025279	2.7666817541344493
4	1.664285943853253	1.894207132982625
5	1.0311063223347419	1.466942142547295
6	0.7689447030812412	1.3127616803096578
7	0.6143481688152689	1.2236357001359037
8	0.5453546875628382	1.2413909316512552
9	0.4887649100735139	1.2516475672179408
>10	8.83865790679075	57.10004841631237
>50	0.15425851499757268	2.6815662893538947
>100	0.030766241128975334	1.5141031475050188
>500	0.0021365445228455093	0.3754557551991888
>1k	0.0017092356182764074	0.8343191036842078
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	2352	0.28536520558549006	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	1750	0.21232530177491818	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1404	0.1703455563954201	No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	1356	0.16452177668959375	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6398623161414546E-4	0.0	0.0	0.19194207280452605	0.0
2	3.6398623161414546E-4	0.0	0.0	0.9457575584774214	0.0
3	3.6398623161414546E-4	0.0	0.0	1.3093798038599527	0.0
4	3.6398623161414546E-4	0.0	0.0	1.6581999424901754	0.0
5	3.6398623161414546E-4	0.0	0.0	2.561735098100356	0.0
6	3.6398623161414546E-4	0.0	0.0	3.1282190032358375	0.0
7	3.6398623161414546E-4	0.0	0.0	3.691669689774535	0.0
8	3.6398623161414546E-4	0.0	0.0	4.534055158473539	0.0
9	3.6398623161414546E-4	0.0	0.0	4.867587875375968	0.0
10	3.6398623161414546E-4	0.0	0.0	5.561709619064143	0.0
11	3.6398623161414546E-4	0.0	0.0	6.644568658116226	0.0
12	3.6398623161414546E-4	0.0	0.0	7.4493422162151015	0.0
13	3.6398623161414546E-4	0.0	0.0	7.784937521763344	0.0
14	3.6398623161414546E-4	0.0	0.0	7.946304751112281	0.0
15	3.6398623161414546E-4	0.0	0.0	8.242832201133938	0.0
16	3.6398623161414546E-4	0.0	0.0	8.821812966888173	0.0
17	3.6398623161414546E-4	0.0	0.0	9.456119639847756	0.0
18	3.6398623161414546E-4	0.0	0.0	10.095764777537681	0.0
19	3.6398623161414546E-4	0.0	0.0	10.526481818281088	0.0
20	3.6398623161414546E-4	0.0	0.0	10.965691871095489	0.0
21	3.6398623161414546E-4	0.0	0.0	11.508759328663794	0.0
22	3.6398623161414546E-4	0.0	0.0	12.10970059705875	0.0
23	3.6398623161414546E-4	0.0	0.0	12.686618774167169	0.0
24	3.6398623161414546E-4	0.0	0.0	13.162227450142986	0.0
25	3.6398623161414546E-4	0.0	0.0	13.583480848864424	0.0
26	3.6398623161414546E-4	0.0	0.0	13.97464471910576	0.0
27	3.6398623161414546E-4	0.0	0.0	14.347245291534772	0.0
28	3.6398623161414546E-4	0.0	0.0	14.729794820961239	0.0
29	3.6398623161414546E-4	0.0	0.0	15.15578004069366	0.0
30	3.6398623161414546E-4	0.0	0.0	15.632965990339805	0.0
31	3.6398623161414546E-4	0.0	0.0	16.069142824557424	0.0
32	3.6398623161414546E-4	0.0	0.0	16.51296336963894	0.0
33	3.6398623161414546E-4	0.0	0.0	16.937371315701032	0.0
34	3.6398623161414546E-4	0.0	0.0	17.362021919250868	0.0
35	3.6398623161414546E-4	0.0	0.0	17.804871834381412	0.0
36	3.6398623161414546E-4	0.0	0.0	18.26810497848235	0.0
37	3.6398623161414546E-4	0.0	0.0	18.729639520169084	0.0
38	3.6398623161414546E-4	0.0	0.0	19.192508678038404	0.0
39	3.6398623161414546E-4	0.0	0.0	19.65913902696774	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCA	20	7.0321246E-4	45.0	1
ACGATAG	30	2.164752E-6	44.999996	1
CGTTAGG	100	0.0	40.5	2
ACGGGCC	105	0.0	38.57143	5
ACGCACC	35	6.2461404E-6	38.571426	38
GCCGATA	35	6.2461404E-6	38.571426	9
TTAAGCG	35	6.2461404E-6	38.571426	1
GACCTAT	125	0.0	37.8	33
CGAAACG	30	1.13964205E-4	37.499996	42
TAGCCGT	30	1.13964205E-4	37.499996	44
TTACGGC	30	1.13964205E-4	37.499996	40
TACGAAT	145	0.0	37.24138	12
TATGCGG	85	0.0	37.058823	2
CGTGCGG	135	0.0	36.666668	2
TAGTTAG	105	0.0	36.42857	1
CTCACGA	25	0.0021067637	36.0	24
TCCGAAC	25	0.0021067637	36.0	36
GTATGCG	25	0.0021067637	36.0	1
CTACGAC	25	0.0021067637	36.0	24
GTACTAC	50	4.876165E-8	36.0	30
>>END_MODULE