FastQCFastQC Report
Sat 18 Jun 2016
SRR3550204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422755
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT681914.792884228134851No Hit
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC29840.20973393170292848Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC28450.19996415405322773Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT18690.13136485199489722Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA14250.1001577924519682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT351.2121927E-745.0000049
TAGTACG351.2121927E-745.0000041
CATATCG207.0338533E-445.01
CGCATCG253.8912753E-545.021
TTATACG406.8175723E-945.01
CGCGTAG207.0338533E-445.01
ATACCGG207.0338533E-445.02
TATCCGA207.0338533E-445.030
ACGATAG302.1658689E-644.9999961
ATAACGC302.1658689E-644.99999611
CGTTTTT370350.044.3499371
ATAGCGG1050.040.7142832
GTTTTTT409700.040.3429342
TTAGCGC403.4589902E-739.37519
ATATGCG403.4589902E-739.3751
TCTAGCG403.4589902E-739.3751
ATATCGG403.4589902E-739.3752
AACGCAC403.4589902E-739.3751
GCAATTG403.4589902E-739.3751
GTACGTG356.2493364E-638.571431