##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550204_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1422755
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51546893175564	34.0	31.0	34.0	31.0	34.0
2	32.606274446408555	34.0	31.0	34.0	31.0	34.0
3	32.69731752831654	34.0	31.0	34.0	31.0	34.0
4	36.16815825634069	37.0	37.0	37.0	35.0	37.0
5	36.132808529929605	37.0	35.0	37.0	35.0	37.0
6	36.14764242613802	37.0	36.0	37.0	35.0	37.0
7	36.294236182617524	37.0	37.0	37.0	35.0	37.0
8	36.233986877572036	37.0	37.0	37.0	35.0	37.0
9	37.89997996844151	39.0	39.0	39.0	35.0	39.0
10	37.60437882839983	39.0	38.0	39.0	35.0	39.0
11	37.54664857969222	39.0	37.0	39.0	35.0	39.0
12	37.48841156769788	39.0	37.0	39.0	35.0	39.0
13	37.478941209132984	39.0	37.0	39.0	35.0	39.0
14	38.78560960952518	40.0	38.0	41.0	35.0	41.0
15	38.819380005693176	40.0	38.0	41.0	35.0	41.0
16	38.87884491707989	40.0	38.0	41.0	35.0	41.0
17	38.86500908448749	40.0	38.0	41.0	35.0	41.0
18	38.36519920857772	39.0	38.0	41.0	35.0	41.0
19	37.88463860608467	38.0	37.0	41.0	35.0	41.0
20	37.348997543498356	38.0	35.0	41.0	34.0	41.0
21	37.286067523923656	38.0	35.0	41.0	34.0	41.0
22	37.23979181236404	38.0	35.0	41.0	34.0	41.0
23	37.181494354263386	38.0	35.0	41.0	34.0	41.0
24	37.14023918383699	38.0	35.0	41.0	34.0	41.0
25	37.06916018569606	38.0	35.0	41.0	34.0	41.0
26	36.97573510548197	38.0	35.0	41.0	33.0	41.0
27	36.92691292597812	38.0	35.0	40.0	33.0	41.0
28	36.819827728596984	38.0	35.0	40.0	33.0	41.0
29	36.64773204100495	38.0	35.0	40.0	33.0	41.0
30	36.375330257141954	38.0	35.0	40.0	33.0	41.0
31	35.94613900495869	38.0	35.0	40.0	31.0	41.0
32	35.27363671187239	38.0	35.0	41.0	25.0	41.0
33	34.43227751791419	38.0	35.0	41.0	18.0	41.0
34	33.65120206922485	38.0	34.0	41.0	15.0	41.0
35	33.19649834300354	38.0	33.0	41.0	10.0	41.0
36	32.94075859863434	38.0	33.0	41.0	10.0	41.0
37	32.69162505139676	37.0	32.0	40.0	10.0	41.0
38	32.51335964378969	37.0	32.0	40.0	10.0	41.0
39	32.394152190644206	37.0	31.0	40.0	9.0	41.0
40	32.21674568003627	37.0	31.0	40.0	8.0	41.0
41	32.0593601849932	37.0	31.0	40.0	8.0	41.0
42	31.94685451817073	37.0	30.0	40.0	8.0	41.0
43	31.829144863310972	36.0	30.0	40.0	8.0	41.0
44	31.618499319981304	36.0	29.0	40.0	7.0	41.0
45	31.648324553419247	36.0	29.0	40.0	8.0	41.0
46	31.48212728122551	36.0	28.0	40.0	8.0	41.0
47	31.512226630727007	35.0	29.0	40.0	8.0	41.0
48	31.480563413939855	35.0	29.0	40.0	8.0	41.0
49	31.43187126385077	35.0	28.0	40.0	8.0	41.0
50	31.282412643076285	35.0	28.0	40.0	8.0	41.0
51	30.56045137778465	35.0	26.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	9.0
11	16.0
12	21.0
13	24.0
14	37.0
15	56.0
16	101.0
17	270.0
18	541.0
19	1027.0
20	1722.0
21	2688.0
22	4170.0
23	7164.0
24	13351.0
25	27522.0
26	51057.0
27	65734.0
28	64053.0
29	51862.0
30	41910.0
31	38151.0
32	37298.0
33	43518.0
34	72552.0
35	95687.0
36	107075.0
37	124649.0
38	222685.0
39	347550.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.98654019841786	15.773833161717935	18.24207259858514	35.997554041279066
2	50.04361256857295	18.09601793703062	18.6836103194155	13.176759174980933
3	24.266651672283704	17.94497295739604	44.7788269941065	13.009548376213754
4	21.599994377106388	19.67900306096271	44.344809893481305	14.376192668449592
5	19.213708614624444	23.14572783086336	43.062649577755835	14.577913976756363
6	20.379334460254928	25.73703835164874	42.28479253279728	11.59883465529905
7	59.84276983739295	5.314548182926786	31.217953899301005	3.6247280803792643
8	60.24065984656529	4.672554304852206	31.208957269522863	3.877828579059641
9	55.345298382363794	6.573303203995066	32.94931312840229	5.13208528523885
10	30.983971238899176	19.755825844927625	37.91165731274886	11.348545603424343
11	23.81935048550172	19.127326911520253	42.35177525294235	14.701547350035671
12	21.770086908849382	17.116720728445866	46.090085784270656	15.023106578434096
13	19.061117339246746	18.11309747637506	46.6061971316214	16.2195880527568
14	16.07156537843831	20.884270306553134	46.30600489894606	16.738159416062498
15	15.606130359759762	19.357092401713576	49.488422110623404	15.548355127903257
16	17.449666316407253	18.734497506598114	47.47872964776086	16.337106529233775
17	16.68263334165053	19.02941827651282	45.861515158969745	18.426433222866905
18	16.384971411100295	20.08075880949285	46.91264483343935	16.621624945967504
19	18.98183453932687	20.01342465849707	44.578230264522	16.42651053765406
20	20.018485262747276	19.349360922997985	45.374502286057684	15.257651528197055
21	19.09548727644605	19.765244191726612	45.34006206268823	15.799206469139099
22	17.846150602176763	18.804291673548853	44.931277697143926	18.418280027130464
23	18.297458100656826	19.61244205783849	44.99903356516055	17.091066276344137
24	18.346798992096318	18.2088272401081	46.25061939687438	17.193754370921205
25	17.935976327617897	19.98657534150293	44.1255170426391	17.95193128824007
26	16.347016879223762	21.06771721062305	45.01934626833151	17.565919641821676
27	17.296091034647567	20.65176365572428	45.28362226806442	16.768523041563725
28	16.048441228461684	21.059634301056754	45.919922966357525	16.97200150412404
29	16.892226701013175	20.396976288960502	45.24658145639973	17.46421555362659
30	17.27135030275768	20.14436779347112	44.20170725107274	18.382574652698462
31	17.931407726558685	21.614754472836154	42.732164005749404	17.721673794855754
32	18.80450253205928	22.648874894131456	41.481702752757855	17.06491982105141
33	18.92911991172057	23.326925577488744	38.8872293543161	18.856725156474585
34	17.7803627469241	24.13184279795186	39.44241981226564	18.645374642858396
35	19.030683427575372	24.17527965109945	36.78201798623094	20.012018935094236
36	19.546162199394836	25.844154474944737	35.55693004066055	19.052753284999877
37	19.328064213445042	27.086111101349143	35.77601203299233	17.809812652213488
38	19.109052507283405	26.707128071944926	34.73170011702647	19.4521193037452
39	20.240940991245857	25.32199851696181	34.850202599885435	19.5868578919069
40	20.038024818046676	24.601846417689625	36.520061430112705	18.840067334150994
41	18.45764028240983	26.07384967896792	35.12312379854578	20.345386240076472
42	19.797786688502235	25.318905925475573	34.93440543171523	19.94890195430696
43	20.31368717734255	24.504008068852333	34.84563399882622	20.336670754978897
44	18.53425220786432	25.286082284019386	34.82532129565526	21.354344212461037
45	18.71727739491339	25.027077747047098	34.23442546327372	22.02121939476579
46	19.087544939220034	25.208697210693337	34.7888427733517	20.91491507673493
47	18.47985071217462	24.85213546956433	36.81779364683308	19.850220171427967
48	18.667585072623186	23.60511823890972	36.6008905257757	21.126406162691396
49	18.855811436262744	23.62079205485133	37.19382465709135	20.329571851794583
50	17.659470534280324	23.395032876356083	37.02612185513318	21.919374734230416
51	17.517070753573176	22.889394168356464	36.137564092201394	23.455970985868966
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	623.0
1	1364.5
2	2106.0
3	62131.0
4	122156.0
5	81806.0
6	41456.0
7	39075.0
8	36694.0
9	34892.5
10	33091.0
11	31426.0
12	29761.0
13	27536.0
14	25311.0
15	23316.0
16	21321.0
17	19690.5
18	18060.0
19	16676.0
20	15292.0
21	14580.0
22	13868.0
23	13582.0
24	13296.0
25	13456.5
26	13778.0
27	13939.0
28	15710.0
29	17481.0
30	19992.0
31	22503.0
32	23043.0
33	23583.0
34	25740.5
35	27898.0
36	29995.0
37	32092.0
38	33127.5
39	34163.0
40	39309.0
41	44455.0
42	49969.0
43	55483.0
44	61201.0
45	66919.0
46	73894.5
47	80870.0
48	88891.5
49	96913.0
50	93985.0
51	91057.0
52	79973.5
53	68890.0
54	63080.5
55	57271.0
56	55529.5
57	53788.0
58	52930.0
59	52072.0
60	50028.0
61	47984.0
62	45495.0
63	43006.0
64	36775.0
65	30544.0
66	26889.0
67	23234.0
68	20356.0
69	17478.0
70	15140.5
71	12803.0
72	10982.0
73	9161.0
74	7302.0
75	4289.0
76	3135.0
77	2629.5
78	2124.0
79	1550.0
80	976.0
81	705.0
82	434.0
83	379.0
84	324.0
85	181.5
86	39.0
87	30.5
88	22.0
89	15.5
90	9.0
91	7.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1422755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.839379230009055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8319524754306	23.22457146126416
2	9.550258720151783	5.6994758339062
3	3.6005803627317943	3.2231724867503266
4	1.7533143744205604	2.0927125011104475
5	1.0227293525143222	1.525880449966824
6	0.6822788318235682	1.2215266082034615
7	0.46031112877610986	0.9614778834740715
8	0.3527242196509154	0.8420057403018142
9	0.29181829919184604	0.7836909206257566
>10	3.188706284226603	23.341342696851815
>50	1.0992070351403875	22.856046677384267
>100	0.15901981700158804	7.31853755342067
>500	0.004496095995320418	0.9552948907291242
>1k	0.0023663663133265357	1.1392362899714459
>5k	0.0	0.0
>10k+	2.3663663133265358E-4	4.815028006039597
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68191	4.792884228134851	No Hit
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	2984	0.20973393170292848	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	2845	0.19996415405322773	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	1869	0.13136485199489722	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1425	0.1001577924519682	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.622893611338565E-4	0.0	0.0	0.13740946262708617	0.0
2	5.622893611338565E-4	0.0	0.0	0.7091171705599347	0.0
3	6.325755312755885E-4	0.0	0.0	1.0173220266314298	0.0
4	6.325755312755885E-4	0.0	0.0	1.340989840134106	0.0
5	6.325755312755885E-4	0.0	0.0	2.2222378413711428	0.0
6	6.325755312755885E-4	0.0	0.0	2.675935069636023	0.0
7	6.325755312755885E-4	0.0	0.0	3.1568330457457536	0.0
8	6.325755312755885E-4	0.0	0.0	3.87979659182361	0.0
9	6.325755312755885E-4	0.0	0.0	4.157216105373026	0.0
10	6.325755312755885E-4	0.0	0.0	4.772922955814599	0.0
11	6.325755312755885E-4	0.0	0.0	5.648337204929872	0.0
12	6.325755312755885E-4	0.0	0.0	6.389364296734153	0.0
13	6.325755312755885E-4	0.0	0.0	6.6739529996380265	0.0
14	6.325755312755885E-4	0.0	0.0	6.787254305906498	0.0
15	6.325755312755885E-4	0.0	0.0	7.00767173547097	0.0
16	6.325755312755885E-4	0.0	0.0	7.467202715857614	0.0
17	6.325755312755885E-4	0.0	0.0	8.039542999321739	0.0
18	6.325755312755885E-4	0.0	0.0	8.672610533788319	0.0
19	6.325755312755885E-4	0.0	0.0	9.023479095135846	7.028617014173207E-5
20	6.325755312755885E-4	0.0	0.0	9.384539151153923	7.028617014173207E-5
21	6.325755312755885E-4	0.0	0.0	9.82467114858145	7.028617014173207E-5
22	6.325755312755885E-4	0.0	0.0	10.288630157687022	7.028617014173207E-5
23	6.325755312755885E-4	0.0	0.0	10.742819389142895	7.028617014173207E-5
24	6.325755312755885E-4	0.0	0.0	11.116390383446202	7.028617014173207E-5
25	7.028617014173206E-4	0.0	0.0	11.445399945879648	7.028617014173207E-5
26	7.028617014173206E-4	0.0	0.0	11.744467599832719	7.028617014173207E-5
27	7.028617014173206E-4	0.0	0.0	12.048174141015144	7.028617014173207E-5
28	7.028617014173206E-4	0.0	0.0	12.352513257728843	7.028617014173207E-5
29	7.028617014173206E-4	0.0	0.0	12.673088479745283	7.028617014173207E-5
30	7.028617014173206E-4	0.0	0.0	13.026557629388053	7.028617014173207E-5
31	7.731478715590526E-4	0.0	0.0	13.355567191821502	7.028617014173207E-5
32	8.434340417007848E-4	0.0	0.0	13.706857470189878	7.028617014173207E-5
33	8.434340417007848E-4	0.0	0.0	14.014078319879388	7.028617014173207E-5
34	9.137202118425168E-4	0.0	0.0	14.344493605715671	7.028617014173207E-5
35	9.840063819842489E-4	0.0	0.0	14.683835235159954	7.028617014173207E-5
36	9.840063819842489E-4	0.0	0.0	15.02331743694452	7.028617014173207E-5
37	0.0010542925521259809	0.0	0.0	15.367649384468866	7.028617014173207E-5
38	0.0010542925521259809	0.0	0.0	15.70024354157954	7.028617014173207E-5
39	0.0010542925521259809	0.0	0.0	16.03733601357929	7.028617014173207E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	35	1.2121927E-7	45.000004	9
TAGTACG	35	1.2121927E-7	45.000004	1
CATATCG	20	7.0338533E-4	45.0	1
CGCATCG	25	3.8912753E-5	45.0	21
TTATACG	40	6.8175723E-9	45.0	1
CGCGTAG	20	7.0338533E-4	45.0	1
ATACCGG	20	7.0338533E-4	45.0	2
TATCCGA	20	7.0338533E-4	45.0	30
ACGATAG	30	2.1658689E-6	44.999996	1
ATAACGC	30	2.1658689E-6	44.999996	11
CGTTTTT	37035	0.0	44.349937	1
ATAGCGG	105	0.0	40.714283	2
GTTTTTT	40970	0.0	40.342934	2
TTAGCGC	40	3.4589902E-7	39.375	19
ATATGCG	40	3.4589902E-7	39.375	1
TCTAGCG	40	3.4589902E-7	39.375	1
ATATCGG	40	3.4589902E-7	39.375	2
AACGCAC	40	3.4589902E-7	39.375	1
GCAATTG	40	3.4589902E-7	39.375	1
GTACGTG	35	6.2493364E-6	38.57143	1
>>END_MODULE