FastQCFastQC Report
Sat 18 Jun 2016
SRR3550201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360452
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC42140.30974999485465127No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC41520.3051926859602544No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG35680.2622657763743227No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC31120.22874750450585538No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC21210.1559040671776733No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT17740.1303978383654844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATC302.165796E-645.00000416
ATCGCTA207.0337445E-445.033
CCCGCGA253.8911854E-545.028
TATAGCG700.041.7857131
GTATACG501.0822987E-940.51
TAGCGCG451.9286745E-840.01
AATTGCG451.9286745E-840.01
AATAGCG800.039.3751
TACGGCT4400.038.8636327
TCGATCA356.2491363E-638.57142617
CCGCTAT659.094947E-1238.0769225
CGCTATC659.094947E-1238.0769226
ACGGGAC2850.037.8947375
GCAACCG301.14003196E-437.5000041
CGTTATA301.14003196E-437.50000423
TCATCGA301.14003196E-437.50000416
CATCGAT301.14003196E-437.50000411
GACCGAT6200.036.6532259
ATTGTCG250.002107244536.01
TCTAGCG250.002107244536.01