##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550201_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1360452
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08796708740918	33.0	31.0	34.0	30.0	34.0
2	32.23123932340134	34.0	31.0	34.0	30.0	34.0
3	32.21125699399905	34.0	31.0	34.0	30.0	34.0
4	35.810570310455645	37.0	35.0	37.0	35.0	37.0
5	35.79421471687351	37.0	35.0	37.0	35.0	37.0
6	35.719753434887814	37.0	35.0	37.0	35.0	37.0
7	35.94083730995287	37.0	35.0	37.0	35.0	37.0
8	35.73970121694848	37.0	35.0	37.0	35.0	37.0
9	37.574712669024706	39.0	37.0	39.0	35.0	39.0
10	37.30365716688277	39.0	37.0	39.0	34.0	39.0
11	37.1990140041692	39.0	37.0	39.0	34.0	39.0
12	37.033116934665834	39.0	37.0	39.0	33.0	39.0
13	36.96834360932984	39.0	37.0	39.0	33.0	39.0
14	38.08432491554277	40.0	37.0	41.0	33.0	41.0
15	38.220703854307246	40.0	37.0	41.0	33.0	41.0
16	38.27920205931558	40.0	37.0	41.0	34.0	41.0
17	38.20446439859694	40.0	37.0	41.0	34.0	41.0
18	38.11452811271548	40.0	37.0	41.0	33.0	41.0
19	38.05094483304078	40.0	37.0	41.0	34.0	41.0
20	37.98985116711211	40.0	36.0	41.0	33.0	41.0
21	37.86003989850432	40.0	36.0	41.0	33.0	41.0
22	37.91052826560584	40.0	36.0	41.0	33.0	41.0
23	37.9247382487585	40.0	36.0	41.0	33.0	41.0
24	37.895597198578116	40.0	36.0	41.0	33.0	41.0
25	37.78063467141803	40.0	36.0	41.0	33.0	41.0
26	37.781364575890954	40.0	36.0	41.0	33.0	41.0
27	36.84065883985617	40.0	36.0	41.0	32.0	41.0
28	37.14036290879796	40.0	35.0	41.0	32.0	41.0
29	37.40588275073284	40.0	36.0	41.0	32.0	41.0
30	37.450650960122076	40.0	36.0	41.0	33.0	41.0
31	37.49463340125194	40.0	36.0	41.0	33.0	41.0
32	37.42678462746205	40.0	36.0	41.0	33.0	41.0
33	37.355028328820126	40.0	35.0	41.0	33.0	41.0
34	37.239267537553694	40.0	35.0	41.0	32.0	41.0
35	37.1754909397759	39.0	35.0	41.0	32.0	41.0
36	37.104013225016395	39.0	35.0	41.0	32.0	41.0
37	37.041395065757555	39.0	35.0	41.0	32.0	41.0
38	36.967947417475955	39.0	35.0	41.0	31.0	41.0
39	36.9209402463299	39.0	35.0	41.0	31.0	41.0
40	36.81240940510948	39.0	35.0	41.0	31.0	41.0
41	36.71778791166465	39.0	35.0	41.0	31.0	41.0
42	36.68609917880234	39.0	35.0	41.0	31.0	41.0
43	36.61332336605775	39.0	35.0	41.0	31.0	41.0
44	36.56483874477012	39.0	35.0	41.0	31.0	41.0
45	36.46936826878126	39.0	35.0	40.0	31.0	41.0
46	36.42568499292882	39.0	35.0	40.0	31.0	41.0
47	36.36959922143523	38.0	35.0	40.0	30.0	41.0
48	36.32087423885591	38.0	35.0	40.0	30.0	41.0
49	36.25871181048652	38.0	35.0	40.0	30.0	41.0
50	36.16076348154878	38.0	35.0	40.0	30.0	41.0
51	34.89231593617416	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	42.0
9	68.0
10	100.0
11	59.0
12	41.0
13	19.0
14	25.0
15	46.0
16	86.0
17	156.0
18	319.0
19	570.0
20	1052.0
21	1696.0
22	2516.0
23	3797.0
24	5278.0
25	7169.0
26	9273.0
27	11313.0
28	13603.0
29	16487.0
30	21307.0
31	27422.0
32	36477.0
33	50948.0
34	88042.0
35	112744.0
36	105740.0
37	156680.0
38	265457.0
39	421802.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.850020434385044	20.35948346578931	23.30872386530359	11.481772234522056
2	30.958166844548725	26.07927365316821	25.509757051332937	17.452802450950124
3	32.78741183077389	25.636259125643534	25.50468520756337	16.071643836019206
4	28.937367874794557	26.52743352944463	26.36395844910368	18.17124014665714
5	26.959936844519323	30.299268184397537	23.9791628076551	18.76163216342804
6	28.57616439242252	35.370009379235725	22.44651042447657	13.607315803865186
7	82.01053767424355	6.006312607868561	7.885246962039087	4.097902755848792
8	83.3525181336791	5.579101651509939	6.890357028399386	4.1780231864115756
9	76.54147298103865	7.550064243354415	9.146518951054503	6.761943824552429
10	42.7904843390285	25.58201244880378	17.2428722218792	14.384630990288521
11	32.3435152434632	25.76731850884853	23.15723009705598	18.73193615063229
12	28.705900685948492	24.327723433094295	27.37296133931958	19.59341454163763
13	25.30409011122774	25.244771590618413	29.423897351762502	20.02724094639135
14	21.716238426640558	28.753384904428824	28.38005309999912	21.150323568931505
15	22.02922264071059	27.008964667625175	31.91909747642695	19.042715215237287
16	24.701496267417006	25.908815599521333	29.007050597889528	20.382637535172137
17	25.32158429698365	25.347237535760176	27.4102283652786	21.920949801977578
18	25.044029484318447	25.795176897090084	28.19812826913408	20.962665349457385
19	25.782093010264234	27.410375375242936	26.279427719610837	20.528103894881994
20	27.370976704801052	26.81542604957764	27.340986672076635	18.472610573544674
21	26.131094665596432	27.92601282514929	26.20599624242531	19.736896266828964
22	24.828880401513615	25.545774492595108	27.24373958066878	22.3816055252225
23	24.15219353567785	27.252045643653727	27.438233763484487	21.157527057183938
24	23.86493606536651	25.388032800863243	28.71045799484289	22.036573138927356
25	22.27458227118634	28.0966178887605	27.31378982867459	22.315010011378572
26	22.29442861637162	28.394386571521817	27.923954685648596	21.387230126457972
27	22.71818483856836	29.918512376768895	26.598512847200784	20.764789937461963
28	20.987142508519227	27.314524878496265	29.17853772128675	22.51979489169776
29	22.766771631781204	26.48524166968037	28.804029837142366	21.943956861396067
30	22.835278275161492	27.1495061935298	27.8122271127537	22.20298841855501
31	22.888569387233066	28.161302273068067	25.838397826604687	23.111730513094177
32	24.280533234542638	28.72412992152608	25.822373740492132	21.17296310343915
33	24.357272435925704	27.492112915413408	25.72696427363847	22.42365037502242
34	22.22526042815182	28.208198451691057	27.496376204379132	22.070164915777994
35	23.327467635756353	27.72225701458045	27.024621228826888	21.92565412083631
36	23.9452770108758	28.82159752788044	25.271968434020458	21.961157027223305
37	23.164507090290577	29.72313613416717	26.252965926030463	20.85939084951178
38	21.60539291353168	29.70887616762664	25.36686336599895	23.318867552842732
39	23.135619632298678	28.61203482372035	26.15858552892715	22.09376001505382
40	22.68753326100443	27.016903205699283	27.878822626597632	22.416740906698656
41	21.73086591809193	28.182912737825372	26.741774057445618	23.344447286637088
42	22.712598459923612	27.544007432823797	27.159429366122435	22.583964741130153
43	23.486164892256397	26.498840091381393	26.84240237803318	23.172592638329025
44	21.815911182459946	26.831670650636703	26.931269901473918	24.421148265429434
45	21.701243410278348	26.868864171613556	26.5422815358425	24.8876108822656
46	22.72296266240926	28.210183086209582	26.380497070091412	22.686357181289747
47	21.278883782742795	27.426325956373322	28.496411486770572	22.79837877411331
48	21.60252621922714	26.970007027076292	27.56899912676081	23.858467626935752
49	22.194976375498733	25.37186170478635	29.396112468503116	23.037049451211804
50	21.247276640410686	26.944353788299768	27.876985002043437	23.931384569246102
51	21.249261274929214	26.679441832567413	26.464072234816076	25.6072246576873
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	251.0
1	791.5
2	1332.0
3	2284.0
4	3236.0
5	2641.0
6	2046.0
7	1959.5
8	1873.0
9	1948.0
10	2023.0
11	2001.5
12	1980.0
13	2137.5
14	2295.0
15	2242.5
16	2190.0
17	2399.5
18	2609.0
19	2854.0
20	3099.0
21	3668.5
22	4238.0
23	4563.5
24	4889.0
25	6238.5
26	9557.5
27	11527.0
28	14883.0
29	18239.0
30	21320.5
31	24402.0
32	27836.5
33	31271.0
34	34422.5
35	37574.0
36	42374.0
37	47174.0
38	50321.5
39	53469.0
40	60011.0
41	66553.0
42	71418.0
43	76283.0
44	86387.5
45	96492.0
46	109197.5
47	121903.0
48	122242.0
49	122581.0
50	122328.0
51	122075.0
52	109258.5
53	96442.0
54	87778.0
55	79114.0
56	74529.0
57	69944.0
58	66729.0
59	63514.0
60	58012.0
61	52510.0
62	47650.0
63	42790.0
64	37164.5
65	31539.0
66	26210.0
67	20881.0
68	17178.0
69	13475.0
70	10970.0
71	8465.0
72	7029.0
73	5593.0
74	4328.5
75	2449.5
76	1835.0
77	1479.5
78	1124.0
79	785.5
80	447.0
81	341.5
82	236.0
83	189.5
84	143.0
85	118.5
86	94.0
87	57.5
88	21.0
89	22.0
90	23.0
91	13.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1360452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.32045436453029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96787389241247	16.78323518820276
2	10.90878524426026	5.087956569224655
3	4.03633038382861	2.823871755487264
4	1.9824489543971953	1.849264414841223
5	1.1620430175218992	1.354968557987026
6	0.7803441835144035	1.0918788552164578
7	0.5893939030578796	0.962145353329557
8	0.4285678781058776	0.7995518114777358
9	0.3738300069800825	0.7846097056083949
>10	5.803720381093427	34.541842554293254
>50	1.873883101870286	29.086153522560448
>100	0.08860560074853413	3.0365962157233124
>500	0.0019262087118913334	0.2976702799203278
>1k	0.0022472434972065558	1.5002552161276281
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	4214	0.30974999485465127	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC	4152	0.3051926859602544	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	3568	0.2622657763743227	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	3112	0.22874750450585538	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	2121	0.1559040671776733	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	1774	0.1303978383654844	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.675249108384566E-4	0.0	0.0	0.14451079494168115	0.0
2	3.675249108384566E-4	0.0	0.0	0.7014580448262783	0.0
3	3.675249108384566E-4	0.0	0.0	0.9519630240537703	0.0
4	3.675249108384566E-4	0.0	0.0	1.2888363573282997	0.0
5	3.675249108384566E-4	0.0	0.0	2.3728878343374116	0.0
6	3.675249108384566E-4	0.0	0.0	2.8784551016867925	0.0
7	4.4102989300614793E-4	0.0	0.0	3.3929899768606315	0.0
8	4.4102989300614793E-4	0.0	0.0	4.148400678597995	0.0
9	4.4102989300614793E-4	0.0	0.0	4.381484977051745	0.0
10	4.4102989300614793E-4	0.0	0.0	5.245756557379459	0.0
11	4.4102989300614793E-4	0.0	0.0	6.113409366886888	0.0
12	4.4102989300614793E-4	0.0	0.0	7.136672223643319	0.0
13	4.4102989300614793E-4	0.0	0.0	7.4179757903990735	0.0
14	4.4102989300614793E-4	0.0	0.0	7.539920555815273	0.0
15	4.4102989300614793E-4	0.0	0.0	7.8172548535339725	0.0
16	4.4102989300614793E-4	0.0	0.0	8.337743632263395	0.0
17	4.4102989300614793E-4	0.0	0.0	8.912111562921735	0.0
18	4.4102989300614793E-4	0.0	0.0	9.519042200680362	0.0
19	4.4102989300614793E-4	0.0	0.0	9.940519768429905	0.0
20	5.145348751738393E-4	0.0	0.0	10.322304645808893	0.0
21	5.145348751738393E-4	0.0	0.0	10.811627312099214	0.0
22	5.145348751738393E-4	0.0	0.0	11.357548814658658	0.0
23	5.145348751738393E-4	0.0	0.0	11.871422144992987	0.0
24	5.880398573415306E-4	0.0	0.0	12.300838250816641	0.0
25	5.880398573415306E-4	0.0	0.0	12.710334506472849	0.0
26	5.880398573415306E-4	0.0	0.0	13.06720119489699	0.0
27	5.880398573415306E-4	0.0	0.0	13.42796364737602	0.0
28	5.880398573415306E-4	0.0	0.0	13.802471531520407	0.0
29	5.880398573415306E-4	0.0	0.0	14.191018867258823	0.0
30	5.880398573415306E-4	0.0	0.0	14.64513264709082	0.0
31	6.615448395092219E-4	0.0	0.0	15.072637623378112	0.0
32	6.615448395092219E-4	0.0	0.0	15.488455307500743	0.0
33	7.350498216769132E-4	0.0	0.0	15.912505549626154	0.0
34	7.350498216769132E-4	0.0	0.0	16.30965296827819	0.0
35	7.350498216769132E-4	0.0	0.0	16.729733941366547	0.0
36	7.350498216769132E-4	0.0	0.0	17.15143202406259	0.0
37	8.085548038446046E-4	0.0	0.0	17.577246385760027	0.0
38	8.085548038446046E-4	0.0	0.0	18.007765066316196	0.0
39	8.085548038446046E-4	0.0	0.0	18.432476853281116	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGATC	30	2.165796E-6	45.000004	16
ATCGCTA	20	7.0337445E-4	45.0	33
CCCGCGA	25	3.8911854E-5	45.0	28
TATAGCG	70	0.0	41.785713	1
GTATACG	50	1.0822987E-9	40.5	1
TAGCGCG	45	1.9286745E-8	40.0	1
AATTGCG	45	1.9286745E-8	40.0	1
AATAGCG	80	0.0	39.375	1
TACGGCT	440	0.0	38.863632	7
TCGATCA	35	6.2491363E-6	38.571426	17
CCGCTAT	65	9.094947E-12	38.07692	25
CGCTATC	65	9.094947E-12	38.07692	26
ACGGGAC	285	0.0	37.894737	5
GCAACCG	30	1.14003196E-4	37.500004	1
CGTTATA	30	1.14003196E-4	37.500004	23
TCATCGA	30	1.14003196E-4	37.500004	16
CATCGAT	30	1.14003196E-4	37.500004	11
GACCGAT	620	0.0	36.653225	9
ATTGTCG	25	0.0021072445	36.0	1
TCTAGCG	25	0.0021072445	36.0	1
>>END_MODULE