##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	996686
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0678107247418	33.0	31.0	34.0	30.0	34.0
2	32.208010346287594	34.0	31.0	34.0	30.0	34.0
3	32.19410125154763	34.0	31.0	34.0	30.0	34.0
4	35.787005135017445	37.0	35.0	37.0	35.0	37.0
5	35.781940350320966	37.0	35.0	37.0	35.0	37.0
6	35.70114559650683	37.0	35.0	37.0	35.0	37.0
7	35.95144809900009	37.0	35.0	37.0	35.0	37.0
8	35.8412177957752	37.0	35.0	37.0	35.0	37.0
9	37.642271487710275	39.0	37.0	39.0	35.0	39.0
10	37.326138824062944	39.0	37.0	39.0	34.0	39.0
11	37.188840818472414	39.0	37.0	39.0	34.0	39.0
12	36.97691248798518	39.0	37.0	39.0	33.0	39.0
13	36.8683045613162	39.0	35.0	39.0	33.0	39.0
14	37.9227399602282	40.0	37.0	41.0	33.0	41.0
15	38.09585566567605	40.0	37.0	41.0	33.0	41.0
16	38.16551050180298	40.0	37.0	41.0	34.0	41.0
17	38.13219208456826	40.0	37.0	41.0	34.0	41.0
18	38.049884316625295	40.0	36.0	41.0	34.0	41.0
19	38.00918142724991	40.0	36.0	41.0	34.0	41.0
20	37.948550496344886	40.0	36.0	41.0	34.0	41.0
21	37.772671633794396	40.0	36.0	41.0	33.0	41.0
22	37.836578420886816	40.0	36.0	41.0	33.0	41.0
23	37.882557796537725	40.0	36.0	41.0	34.0	41.0
24	37.86959182731572	40.0	36.0	41.0	33.0	41.0
25	37.73391820493114	40.0	36.0	41.0	33.0	41.0
26	37.74133679012247	40.0	36.0	41.0	33.0	41.0
27	36.765912233140625	39.0	35.0	41.0	32.0	41.0
28	37.09111595828576	39.0	35.0	41.0	32.0	41.0
29	37.37086605009	39.0	35.0	41.0	32.0	41.0
30	37.41515381975868	39.0	36.0	41.0	33.0	41.0
31	37.43365212313607	39.0	35.0	41.0	33.0	41.0
32	37.35566567605043	39.0	35.0	41.0	33.0	41.0
33	37.26803225890601	39.0	35.0	41.0	32.0	41.0
34	37.163530941540266	39.0	35.0	41.0	32.0	41.0
35	37.11703284685448	39.0	35.0	41.0	32.0	41.0
36	37.04850875802409	39.0	35.0	41.0	32.0	41.0
37	36.9852440989439	39.0	35.0	41.0	32.0	41.0
38	36.89830096941263	39.0	35.0	41.0	31.0	41.0
39	36.84828923050991	39.0	35.0	41.0	31.0	41.0
40	36.748026961349915	39.0	35.0	41.0	31.0	41.0
41	36.69146852669748	39.0	35.0	41.0	31.0	41.0
42	36.656037106972505	39.0	35.0	41.0	31.0	41.0
43	36.56049548202744	39.0	35.0	41.0	31.0	41.0
44	36.471299887828266	39.0	35.0	40.0	31.0	41.0
45	36.362742127410236	38.0	35.0	40.0	30.0	41.0
46	36.32830600610423	38.0	35.0	40.0	30.0	41.0
47	36.30046273349882	38.0	35.0	40.0	31.0	41.0
48	36.257043843296685	38.0	35.0	40.0	31.0	41.0
49	36.18830504291221	38.0	35.0	40.0	30.0	41.0
50	36.06605691260838	38.0	35.0	40.0	30.0	41.0
51	34.74025119245179	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	26.0
9	57.0
10	69.0
11	50.0
12	27.0
13	20.0
14	19.0
15	31.0
16	61.0
17	98.0
18	234.0
19	393.0
20	684.0
21	1040.0
22	1720.0
23	2540.0
24	3854.0
25	5173.0
26	6761.0
27	8253.0
28	9887.0
29	12523.0
30	15615.0
31	20476.0
32	27250.0
33	38539.0
34	67863.0
35	92854.0
36	76763.0
37	113317.0
38	190897.0
39	299475.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31080400447081	22.311640777536756	25.549169949211688	10.828385268780739
2	30.40887501178907	24.542032295025713	27.285524227289237	17.76356846589598
3	31.75072189235125	24.679387490142332	26.912989647692452	16.656900969813965
4	28.81990917901927	25.86872896779929	26.8735589744413	18.437802878740143
5	25.881972858051583	30.698534944807093	24.8057061100487	18.613786087092624
6	27.862335780777496	33.628645330625694	23.706663884111947	14.802355004484863
7	82.23763552412696	5.878982949494625	7.121801650670322	4.7615798757080965
8	83.90315505585511	3.9489869427282014	7.1095610854371385	5.038296915979556
9	77.28753087732746	6.461011793082275	9.751616858268301	6.49984047132196
10	43.24110100874297	22.591167127861734	18.079114184407125	16.088617678988168
11	30.986689890296443	25.521377846182247	24.227590234035596	19.264342029485714
12	27.861934450769848	24.801492144968424	27.3329815006933	20.003591903568427
13	24.49046138904329	27.474149330882547	29.066125138709687	18.96926414136448
14	20.45739580971339	30.72622671533462	28.05196420938992	20.764413265562073
15	18.592916926695064	26.928741850492532	35.07824931824065	19.400091904571752
16	21.57028392091391	25.79970020648429	31.074480829468857	21.55553504313294
17	21.717873031225484	25.731975767694138	27.921030294395628	24.629120906684754
18	22.048067295015684	25.823980671946835	31.13157002305641	20.996382009981076
19	24.24845939443315	26.86713769431897	27.121279921660385	21.763122989587494
20	25.672879924068365	26.973490146344986	27.101313753780026	20.252316175806623
21	23.12925033561222	27.380539106599272	29.088499286635912	20.4017112711526
22	22.409364634398397	25.022825644184827	27.833339687725122	24.734470033691654
23	21.20266563391078	27.08395623094937	27.971898872864674	23.74147926227518
24	21.641218999765222	25.092155403005563	31.007559050694	22.259066546535216
25	21.700515508394822	27.447962547883687	28.139353818554692	22.712168125166805
26	20.0564671320757	29.824839518163195	27.511774019099295	22.60691933066181
27	19.710019002975862	31.04086944132856	27.923137276935766	21.32597427875981
28	18.656126402899208	27.41134118468605	30.978763622645445	22.953768789769295
29	20.129810190972886	26.558916248447357	30.728835360384316	22.582438200195448
30	21.322562973694826	27.595250660689523	29.24100468954114	21.841181676074513
31	22.368529306120482	28.789107100932494	27.059876430490647	21.78248716245638
32	22.75250179093516	29.702333533329455	25.432984911998364	22.112179763737025
33	23.9957218221185	27.57548515781299	26.397782250377748	22.031010769690756
34	20.368300548016123	29.028901780500576	28.251826553197297	22.350971118286
35	20.526926233537946	28.301290476639586	27.893840186377655	23.277943103444816
36	23.944953576151367	27.50133943890052	27.77303985407641	20.78066713087171
37	20.714247014606403	29.160036360498694	29.79614442261655	20.32957220227835
38	21.115677354753654	28.949639103990627	27.0550604703989	22.87962307085682
39	22.828252829878217	27.582909762954433	27.756484991261036	21.832352415906314
40	22.468560810526085	26.07360793670223	28.999504357440557	22.458326895331126
41	20.448666882047103	26.02404368075803	27.847085240486972	25.68020419670789
42	22.660296221678642	25.556995884360774	27.52200793429425	24.260699959666336
43	22.64203570633078	25.23512921822921	28.817802196479132	23.305032878960876
44	20.75648699791108	26.660553072883538	28.831347084237162	23.751612844968225
45	20.367598220502746	27.919525306866955	27.387562381733066	24.325314090897233
46	22.3518741108032	26.995763961769303	28.33309587974548	22.319266047682017
47	19.550991987446398	27.541171442159317	29.218530209113002	23.689306361281286
48	20.65966613356664	25.27205157893258	30.06172455517585	24.006557732324925
49	22.27351442681045	24.0753858286361	30.703651902404566	22.947447842148883
50	21.087684586720393	25.788964628779777	29.614542594157033	23.508808190342794
51	20.90317311570545	25.399373523858067	27.477159305939885	26.220294054496602
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	375.0
1	702.0
2	1029.0
3	1780.0
4	2531.0
5	1981.5
6	1432.0
7	1426.5
8	1421.0
9	1490.0
10	1559.0
11	1669.0
12	1779.0
13	1646.0
14	1513.0
15	1468.0
16	1423.0
17	1577.0
18	1731.0
19	1944.5
20	2158.0
21	2293.0
22	2428.0
23	2727.5
24	3027.0
25	4331.5
26	6887.0
27	8138.0
28	8688.0
29	9238.0
30	11575.5
31	13913.0
32	16191.5
33	18470.0
34	22486.0
35	26502.0
36	29777.0
37	33052.0
38	36391.5
39	39731.0
40	46790.5
41	53850.0
42	64992.5
43	76135.0
44	86342.0
45	96549.0
46	106555.5
47	116562.0
48	111963.5
49	107365.0
50	95292.0
51	83219.0
52	74242.5
53	65266.0
54	57697.0
55	50128.0
56	44942.0
57	39756.0
58	36178.0
59	32600.0
60	30329.5
61	28059.0
62	25347.5
63	22636.0
64	19430.0
65	16224.0
66	13529.5
67	10835.0
68	9168.5
69	7502.0
70	6262.0
71	5022.0
72	4083.5
73	3145.0
74	2470.0
75	1508.5
76	1222.0
77	1027.5
78	833.0
79	608.5
80	384.0
81	340.5
82	297.0
83	214.5
84	132.0
85	93.5
86	55.0
87	34.5
88	14.0
89	11.0
90	8.0
91	5.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	996686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.130095120925198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03462563153923	16.899421782474278
2	12.246597130163618	5.910231073169953
3	5.023317375557083	3.6363937828436623
4	2.415142831982532	2.331105050654367
5	1.4211016052687766	1.7145658455817538
6	0.9027627190343618	1.3070250169154531
7	0.6100542890397161	1.0304467616419721
8	0.45898611517079524	0.8860302894604174
9	0.364308478887035	0.791171842401335
>10	4.434624928225999	28.066654688330573
>50	1.9811489825116653	32.14984100196658
>100	0.10223916577559368	3.6401647584671606
>500	0.002969602325432182	0.46535964544408637
>1k	0.002121144518165844	1.1715884606483873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	3473	0.3484547791380635	No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	3011	0.30210116325502717	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	2602	0.2610651699732915	No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	1292	0.12962959246944375	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1067	0.10705477953939355	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0133000764533666E-4	0.0	0.0	0.2991915206995985	0.0
2	4.0133000764533666E-4	0.0	0.0	1.3028175373186741	0.0
3	4.0133000764533666E-4	0.0	0.0	1.779898583907068	0.0
4	4.0133000764533666E-4	0.0	0.0	2.319386446684312	0.0
5	4.0133000764533666E-4	0.0	0.0	3.7416999937793847	0.0
6	4.0133000764533666E-4	0.0	0.0	4.513858928489013	0.0
7	4.0133000764533666E-4	0.0	0.0	5.2962517783935965	0.0
8	4.0133000764533666E-4	0.0	0.0	6.558835982445825	0.0
9	4.0133000764533666E-4	0.0	0.0	6.98334279803268	0.0
10	4.0133000764533666E-4	0.0	0.0	7.933993253642572	0.0
11	4.0133000764533666E-4	0.0	0.0	9.352694830668836	0.0
12	5.016625095566708E-4	0.0	0.0	10.429663906185098	0.0
13	5.016625095566708E-4	0.0	0.0	10.857381361833115	0.0
14	5.016625095566708E-4	0.0	0.0	11.017010372374047	0.0
15	5.016625095566708E-4	0.0	0.0	11.370381444105767	0.0
16	5.016625095566708E-4	0.0	0.0	12.141035391286724	0.0
17	5.016625095566708E-4	0.0	0.0	13.017138797976495	0.0
18	5.016625095566708E-4	0.0	0.0	14.04634960258296	0.0
19	5.016625095566708E-4	0.0	0.0	14.59978368312588	0.0
20	5.016625095566708E-4	0.0	0.0	15.14960579359999	0.0
21	5.016625095566708E-4	0.0	0.0	15.861464894660907	0.0
22	5.016625095566708E-4	0.0	0.0	16.586969215981764	0.0
23	5.016625095566708E-4	0.0	0.0	17.30565092717265	0.0
24	5.016625095566708E-4	0.0	0.0	17.889385423292794	0.0
25	5.016625095566708E-4	0.0	0.0	18.37639938757041	0.0
26	5.016625095566708E-4	0.0	0.0	18.8171600684669	0.0
27	5.016625095566708E-4	0.0	0.0	19.243171871582422	0.0
28	5.016625095566708E-4	0.0	0.0	19.747944688698347	0.0
29	5.016625095566708E-4	0.0	0.0	20.25813546091748	0.0
30	5.016625095566708E-4	0.0	0.0	20.85360885976125	0.0
31	6.01995011468005E-4	0.0	0.0	21.36580628201861	0.0
32	6.01995011468005E-4	0.0	0.0	21.86516114403132	0.0
33	6.01995011468005E-4	0.0	0.0	22.347860810726747	0.0
34	6.01995011468005E-4	0.0	0.0	22.826948507353368	0.0
35	7.023275133793391E-4	0.0	0.0	23.35730611245668	0.0
36	7.023275133793391E-4	0.0	0.0	23.835791814071833	0.0
37	8.026600152906733E-4	0.0	0.0	24.350798546382713	0.0
38	8.026600152906733E-4	0.0	0.0	24.84543778080559	0.0
39	8.026600152906733E-4	0.0	0.0	25.353320905480764	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGTCG	20	7.032836E-4	45.0	1
ATTAACG	25	3.890432E-5	45.0	1
ATTCGGT	20	7.032836E-4	45.0	12
GCCGCAC	25	3.890432E-5	45.0	37
ATCGTAC	20	7.032836E-4	45.0	16
TCAATCG	20	7.032836E-4	45.0	44
TACGCAA	20	7.032836E-4	45.0	13
CTCGGTT	35	1.2117198E-7	45.0	17
GTCACGC	25	3.890432E-5	45.0	11
TTACGAC	25	3.890432E-5	45.0	32
TCGAGAG	35	1.2117198E-7	45.0	1
ATCGGTA	20	7.032836E-4	45.0	38
CGCAATA	35	1.2117198E-7	45.0	23
CGTCAAG	20	7.032836E-4	45.0	20
TCGAAGT	20	7.032836E-4	45.0	35
TGCAACG	35	1.2117198E-7	45.0	1
TTGTGCG	65	0.0	41.53846	1
TTGCTCG	60	3.6379788E-12	41.249996	1
CGACGGT	115	0.0	41.086956	28
CGACTGG	105	0.0	40.714287	2
>>END_MODULE