FastQCFastQC Report
Sat 18 Jun 2016
SRR3550198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282857
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34401.2161622303849648No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCC13090.46277801150404624No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC9030.3192425854760533Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT8640.3054546997245958Illumina Single End Adapter 1 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6250.22095970755540784No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC5200.18383847668609932No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5190.18348494115401068No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4940.17464655285179437No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC4870.1721718041271738Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG4620.16333341582495747No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC4480.15838391837571633No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3790.13398996666159932No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC3580.12656572048773762Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGT3220.11383844133254611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATGG406.7830115E-945.02
AGCGTTC207.024261E-445.07
CCCTCGT207.024261E-445.014
AGTTTAG253.8833256E-545.01
ATATGCG207.024261E-445.01
TAACGGG406.7830115E-945.03
TAACGCG207.024261E-445.01
ACGGGTA351.2075907E-745.05
ACGGGAG1050.045.05
GCACCTC207.024261E-445.038
ATCGTAA207.024261E-445.013
CGTTAGG253.8833256E-545.02
CGGATAG253.8833256E-545.018
CCATAGG207.024261E-445.02
TGTTACG207.024261E-445.01
GACGGGA502.1827873E-1145.04
TCGTAAA207.024261E-445.014
TTAGTTG207.024261E-445.01
TATAGAG207.024261E-445.01
CGATGCG207.024261E-445.010