##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550198_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	282857
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08540004313134	33.0	31.0	34.0	30.0	34.0
2	32.22496880048929	33.0	31.0	34.0	30.0	34.0
3	32.17235917795918	33.0	31.0	34.0	30.0	34.0
4	35.80742212496067	37.0	35.0	37.0	35.0	37.0
5	35.94257168816752	37.0	35.0	37.0	35.0	37.0
6	35.99036969210591	37.0	35.0	37.0	35.0	37.0
7	36.01458334069866	37.0	35.0	37.0	35.0	37.0
8	35.9838186786963	37.0	35.0	37.0	35.0	37.0
9	37.79039231837996	39.0	38.0	39.0	35.0	39.0
10	37.55889725196831	39.0	37.0	39.0	35.0	39.0
11	37.54797653938209	39.0	37.0	39.0	35.0	39.0
12	37.47245427901731	39.0	37.0	39.0	35.0	39.0
13	37.46924771174127	39.0	37.0	39.0	35.0	39.0
14	38.69756095836412	40.0	38.0	41.0	35.0	41.0
15	38.808514549754825	40.0	38.0	41.0	35.0	41.0
16	38.832710521570974	40.0	38.0	41.0	35.0	41.0
17	38.77781705950357	40.0	38.0	41.0	35.0	41.0
18	38.630463449729014	40.0	38.0	41.0	35.0	41.0
19	38.44575527563398	40.0	37.0	41.0	35.0	41.0
20	38.29399307777428	40.0	36.0	41.0	34.0	41.0
21	38.21789101913688	40.0	36.0	41.0	34.0	41.0
22	38.22157485938124	40.0	36.0	41.0	34.0	41.0
23	38.17885362568365	40.0	36.0	41.0	34.0	41.0
24	38.08199903131264	40.0	36.0	41.0	34.0	41.0
25	37.996761614526065	40.0	35.0	41.0	34.0	41.0
26	38.05367729983702	40.0	36.0	41.0	34.0	41.0
27	37.99996464644679	40.0	35.0	41.0	34.0	41.0
28	37.945141891485804	40.0	35.0	41.0	34.0	41.0
29	37.87506054295987	40.0	35.0	41.0	34.0	41.0
30	37.71964985840902	40.0	35.0	41.0	33.0	41.0
31	37.57151493510855	40.0	35.0	41.0	33.0	41.0
32	37.28767539781586	40.0	35.0	41.0	33.0	41.0
33	37.00089798025151	40.0	35.0	41.0	32.0	41.0
34	36.77775342310779	40.0	35.0	41.0	31.0	41.0
35	36.58898312574905	40.0	35.0	41.0	31.0	41.0
36	36.451853763562504	40.0	35.0	41.0	30.0	41.0
37	36.339995121209654	40.0	35.0	41.0	30.0	41.0
38	36.23661779627162	39.0	35.0	41.0	30.0	41.0
39	36.12230208197075	39.0	35.0	41.0	29.0	41.0
40	36.03225658194777	39.0	35.0	41.0	29.0	41.0
41	35.9137585423023	39.0	35.0	41.0	28.0	41.0
42	35.86792973127764	39.0	35.0	41.0	28.0	41.0
43	35.72712359955737	39.0	35.0	41.0	27.0	41.0
44	35.62644021537385	39.0	35.0	41.0	26.0	41.0
45	35.52995329795621	39.0	35.0	41.0	26.0	41.0
46	35.429467893670655	39.0	35.0	41.0	25.0	41.0
47	35.39591737167544	39.0	35.0	41.0	25.0	41.0
48	35.3545431083551	38.0	35.0	41.0	25.0	41.0
49	35.29594812926673	38.0	35.0	41.0	25.0	41.0
50	35.20015414149199	38.0	35.0	41.0	24.0	41.0
51	34.35592189693025	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	5.0
11	7.0
12	8.0
13	3.0
14	18.0
15	18.0
16	29.0
17	43.0
18	91.0
19	166.0
20	307.0
21	465.0
22	706.0
23	1219.0
24	2012.0
25	2990.0
26	3777.0
27	4117.0
28	4178.0
29	4255.0
30	4380.0
31	4995.0
32	5849.0
33	7864.0
34	14409.0
35	20919.0
36	19002.0
37	27550.0
38	49707.0
39	103631.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.31613147279367	20.981273222865266	23.23824405971922	15.46435124462184
2	34.49729015014654	26.53779118070262	23.192991511611876	15.771927157538968
3	30.22057081847011	25.344255224371327	29.335317846120123	15.09985611103844
4	26.411578995746964	27.07339751181693	29.227843044365176	17.28718044807093
5	23.217385463325993	31.189965247457195	27.264306699144797	18.328342590072015
6	22.033394966361094	36.66764478163878	27.24061981849486	14.05834043350527
7	71.4113491976511	9.892984794436765	13.69879479737111	4.996871210541015
8	70.7138235928402	9.758994827775165	13.660612959905535	5.8665686194791
9	63.96836564058871	12.20581424536073	16.538745726639256	7.287074387411306
10	34.728148852600434	27.23072082359638	22.584910396419392	15.456219927383803
11	27.47819569605843	26.029407085559136	27.33925623194759	19.153140986434842
12	24.707184195547573	23.611224046072753	32.40471333571381	19.27687842266587
13	22.03693032168198	25.700972576248777	33.51941086838933	18.74268623367992
14	17.73970592914441	29.01572172511198	32.18693544794719	21.057636897796414
15	18.10915056017705	26.05344750174116	35.86688680145798	19.970515136623806
16	20.06172730390268	26.06405356770382	33.157036947998456	20.717182180395042
17	19.181070293469844	27.421630010924247	30.4980962111597	22.899203484446204
18	20.462636597291212	26.563245739013	32.78334989058075	20.190767773115038
19	21.101475303775405	28.228044559618464	30.479358827959004	20.191121308647126
20	22.0457687099842	28.055165684427113	30.277843574668474	19.62122203092022
21	20.471474985593428	29.653499824999912	29.719257433968398	20.155767755438262
22	19.566777558978565	28.04915558038161	28.833650926086325	23.550415934553502
23	20.352687046811642	28.404105254598615	29.269560237151637	21.973647461438112
24	20.418091120248043	26.625821528192688	30.927641882647418	22.028445468911855
25	19.10718136726332	28.58405484043174	29.25577235140018	23.05299144090477
26	18.45879720141273	28.68834782239789	29.97733837239312	22.875516603796264
27	20.161777859483767	28.493549744217045	29.86067164680386	21.484000749495326
28	18.446423457789628	28.880671151854116	29.69839883757517	22.974506552781087
29	19.67849478711858	27.710115005108587	28.823751931187847	23.78763827658499
30	21.641324061274776	29.57748968560085	27.431529005822732	21.34965724730164
31	22.770516550765933	29.245873356501694	25.22263900133283	22.76097109139954
32	21.697889746408965	29.394358279978928	24.92849743863507	23.979254534977038
33	21.20223293042067	30.486429538600778	25.211325864305994	23.10001166667256
34	20.209151620783647	29.055317704705914	26.83688224084962	23.898648433660824
35	20.724252891036812	28.702489243681434	25.601629091731866	24.971628773549888
36	21.645212952127753	32.557440685576104	24.588749792297875	21.208596569998267
37	22.040465677002867	32.015470714884195	25.396578483120447	20.547485124992487
38	22.607536670473067	30.41749010984349	24.62657809423136	22.348395125452083
39	21.889152469268925	30.322742587243727	25.61082101556617	22.177283927921177
40	23.452840127697034	28.812438794161004	27.06102376819382	20.673697309948135
41	20.987990397974947	29.24198446564872	25.660315990058578	24.10970914631775
42	21.581223020819706	28.150973813623136	27.309559247252146	22.95824391830501
43	22.211223339001688	26.295619341221887	28.855570129075826	22.6375871907006
44	21.029707590761408	27.06738740777142	27.309205711720058	24.593699289747118
45	21.435566381599184	25.703447324973393	27.69208469297207	25.16890160045535
46	21.533495723987738	27.981276758220584	27.747236235977894	22.73799128181378
47	20.527333599663432	27.40466030538399	29.573954330279967	22.49405176467261
48	20.190767773115038	25.811982733324612	29.802338284009235	24.194911209551115
49	21.054455078007614	25.393396663331647	29.81365142103607	23.738496837624666
50	19.44798962019678	25.237841029212642	30.03673234178401	25.27743700880657
51	20.12430309308237	24.239810222126376	27.842690829641832	27.79319585514942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	511.0
1	589.5
2	668.0
3	3201.5
4	5735.0
5	3833.0
6	1931.0
7	1952.0
8	1973.0
9	2061.5
10	2150.0
11	2146.5
12	2143.0
13	2196.0
14	2249.0
15	2205.5
16	2162.0
17	2017.5
18	1873.0
19	1794.5
20	1716.0
21	1789.5
22	1863.0
23	1934.0
24	2005.0
25	2406.5
26	3253.0
27	3698.0
28	3971.0
29	4244.0
30	4551.0
31	4858.0
32	5657.5
33	6457.0
34	6628.0
35	6799.0
36	7159.0
37	7519.0
38	7985.0
39	8451.0
40	9811.0
41	11171.0
42	12570.5
43	13970.0
44	15879.0
45	17788.0
46	20759.5
47	23731.0
48	25461.0
49	27191.0
50	26022.0
51	24853.0
52	21629.0
53	18405.0
54	16577.0
55	14749.0
56	13310.0
57	11871.0
58	11123.0
59	10375.0
60	10019.0
61	9663.0
62	8923.0
63	8183.0
64	7215.0
65	6247.0
66	5456.5
67	4666.0
68	3763.5
69	2861.0
70	2495.0
71	2129.0
72	1804.0
73	1479.0
74	1149.0
75	618.0
76	417.0
77	338.0
78	259.0
79	189.5
80	120.0
81	97.5
82	75.0
83	43.5
84	12.0
85	9.0
86	6.0
87	4.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	282857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.08990835930818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.88583988602014	27.000351452311
2	10.737811870554397	8.180045402578171
3	4.162390656620135	4.756352359989046
4	2.42021411293981	3.6874293508712688
5	1.7766417294344645	3.3836060330740776
6	1.4029658280356243	3.206330389267071
7	1.2028916199730642	3.2072622099667645
8	0.9979191482445384	3.040851912530668
9	0.8347476078511566	2.861591389758205
>10	5.4800793839311295	33.549681414671774
>50	0.06069078323364111	1.506777792389694
>100	0.030842857053161876	2.51975994507019
>500	0.004974654363413206	1.3002400919741046
>1k	0.0019898617453652824	1.7997202555479521
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3440	1.2161622303849648	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCC	1309	0.46277801150404624	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	903	0.3192425854760533	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	864	0.3054546997245958	Illumina Single End Adapter 1 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	625	0.22095970755540784	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	520	0.18383847668609932	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	519	0.18348494115401068	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	494	0.17464655285179437	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	487	0.1721718041271738	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	462	0.16333341582495747	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC	448	0.15838391837571633	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	379	0.13398996666159932	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	358	0.12656572048773762	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGT	322	0.11383844133254611	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.535355320886526E-4	0.0	0.0	0.3192425854760533	0.0
2	3.535355320886526E-4	0.0	0.0	1.0595459896696917	0.0
3	3.535355320886526E-4	0.0	0.0	1.4431320419858797	0.0
4	3.535355320886526E-4	0.0	0.0	1.9320716828644864	0.0
5	3.535355320886526E-4	0.0	0.0	3.295658230130419	0.0
6	3.535355320886526E-4	0.0	0.0	4.297224392537572	0.0
7	3.535355320886526E-4	0.0	0.0	5.324598648787196	0.0
8	3.535355320886526E-4	0.0	0.0	7.262326900165101	0.0
9	3.535355320886526E-4	0.0	0.0	8.299600151313207	0.0
10	3.535355320886526E-4	0.0	0.0	10.124197032422744	0.0
11	3.535355320886526E-4	0.0	0.0	12.292430450722449	0.0
12	3.535355320886526E-4	0.0	0.0	13.873441350222905	0.0
13	3.535355320886526E-4	0.0	0.0	14.51722955415634	0.0
14	3.535355320886526E-4	0.0	0.0	14.78733070067207	0.0
15	3.535355320886526E-4	0.0	0.0	15.225361224929912	0.0
16	3.535355320886526E-4	0.0	0.0	15.994301007222731	0.0
17	3.535355320886526E-4	0.0	0.0	16.905362073415187	0.0
18	3.535355320886526E-4	0.0	0.0	17.914352481996204	0.0
19	3.535355320886526E-4	0.0	0.0	18.469403267375387	0.0
20	3.535355320886526E-4	0.0	0.0	19.02480758828666	0.0
21	3.535355320886526E-4	0.0	0.0	19.69475742159466	0.0
22	3.535355320886526E-4	0.0	0.0	20.341020374252714	0.0
23	3.535355320886526E-4	0.0	0.0	20.939556030078805	0.0
24	3.535355320886526E-4	0.0	0.0	21.46809165055134	0.0
25	3.535355320886526E-4	0.0	0.0	21.928394913330763	0.0
26	3.535355320886526E-4	0.0	0.0	22.35016280311253	0.0
27	3.535355320886526E-4	0.0	0.0	22.767334730977137	0.0
28	3.535355320886526E-4	0.0	0.0	23.23046627801327	0.0
29	3.535355320886526E-4	0.0	0.0	23.65294123885921	0.0
30	3.535355320886526E-4	0.0	0.0	24.161678869534782	0.0
31	3.535355320886526E-4	0.0	0.0	24.617739705929143	0.0
32	3.535355320886526E-4	0.0	0.0	25.076275291048127	0.0
33	3.535355320886526E-4	0.0	0.0	25.49238661231647	0.0
34	3.535355320886526E-4	0.0	0.0	25.920518141675828	0.0
35	3.535355320886526E-4	0.0	0.0	26.35324563295234	0.0
36	3.535355320886526E-4	0.0	0.0	26.782084233375876	0.0
37	3.535355320886526E-4	0.0	0.0	27.218347079973274	0.0
38	3.535355320886526E-4	0.0	0.0	27.619256373361804	0.0
39	3.535355320886526E-4	0.0	0.0	28.084509133590473	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATGG	40	6.7830115E-9	45.0	2
AGCGTTC	20	7.024261E-4	45.0	7
CCCTCGT	20	7.024261E-4	45.0	14
AGTTTAG	25	3.8833256E-5	45.0	1
ATATGCG	20	7.024261E-4	45.0	1
TAACGGG	40	6.7830115E-9	45.0	3
TAACGCG	20	7.024261E-4	45.0	1
ACGGGTA	35	1.2075907E-7	45.0	5
ACGGGAG	105	0.0	45.0	5
GCACCTC	20	7.024261E-4	45.0	38
ATCGTAA	20	7.024261E-4	45.0	13
CGTTAGG	25	3.8833256E-5	45.0	2
CGGATAG	25	3.8833256E-5	45.0	18
CCATAGG	20	7.024261E-4	45.0	2
TGTTACG	20	7.024261E-4	45.0	1
GACGGGA	50	2.1827873E-11	45.0	4
TCGTAAA	20	7.024261E-4	45.0	14
TTAGTTG	20	7.024261E-4	45.0	1
TATAGAG	20	7.024261E-4	45.0	1
CGATGCG	20	7.024261E-4	45.0	10
>>END_MODULE