FastQCFastQC Report
Sat 18 Jun 2016
SRR3550195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423936
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30590.7215711805555556No Hit
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT18450.4352072010869565Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC17430.4111469655797101No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC12580.296742904589372Illumina PCR Primer Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC11710.2762209390096618Illumina PCR Primer Index 8 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8170.19271776871980678No Hit
TCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC7200.16983695652173914Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGC6780.15992980072463767No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5860.1382284118357488No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTC5610.13233129528985507No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG5590.13185952596618358No Hit
ACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC5220.12313179347826088Illumina PCR Primer Index 8 (95% over 23bp)
TTGTGGGAGCTTGAACTTTTTTTTTAAACCCCTGTTTTACTTTTGAATGAT4930.11629113828502415No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4530.10685575181159421No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4500.10614809782608695No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG253.886625E-545.0000042
CGAAAGG351.2095006E-745.0000042
GCCGATA351.2095006E-745.0000049
GCTACGA351.2095006E-745.00000410
CCTATGC253.886625E-545.00000435
TCACGAC207.028243E-445.025
CGACGGT207.028243E-445.028
ATTACGG207.028243E-445.02
CCTAAGC207.028243E-445.016
GTAGACT207.028243E-445.016
TCTAAGT207.028243E-445.012
AGCGCGC207.028243E-445.020
TCGTCGG207.028243E-445.01
AACGATC207.028243E-445.022
CATAATC207.028243E-445.011
CACGTAG207.028243E-445.01
GACTAAT1050.042.8571439
CGTTTTT19650.041.3358761
TGCACGG603.6379788E-1241.2500042
CATGTGC451.9233994E-840.037