Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550195_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 423936 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 3059 | 0.7215711805555556 | No Hit |
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT | 1845 | 0.4352072010869565 | Illumina PCR Primer Index 8 (95% over 24bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC | 1743 | 0.4111469655797101 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC | 1258 | 0.296742904589372 | Illumina PCR Primer Index 8 (95% over 23bp) |
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC | 1171 | 0.2762209390096618 | Illumina PCR Primer Index 8 (95% over 23bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 817 | 0.19271776871980678 | No Hit |
TCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC | 720 | 0.16983695652173914 | Illumina PCR Primer Index 8 (95% over 23bp) |
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGC | 678 | 0.15992980072463767 | No Hit |
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA | 586 | 0.1382284118357488 | No Hit |
CGTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTC | 561 | 0.13233129528985507 | No Hit |
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG | 559 | 0.13185952596618358 | No Hit |
ACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC | 522 | 0.12313179347826088 | Illumina PCR Primer Index 8 (95% over 23bp) |
TTGTGGGAGCTTGAACTTTTTTTTTAAACCCCTGTTTTACTTTTGAATGAT | 493 | 0.11629113828502415 | No Hit |
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 453 | 0.10685575181159421 | No Hit |
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 450 | 0.10614809782608695 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGG | 25 | 3.886625E-5 | 45.000004 | 2 |
CGAAAGG | 35 | 1.2095006E-7 | 45.000004 | 2 |
GCCGATA | 35 | 1.2095006E-7 | 45.000004 | 9 |
GCTACGA | 35 | 1.2095006E-7 | 45.000004 | 10 |
CCTATGC | 25 | 3.886625E-5 | 45.000004 | 35 |
TCACGAC | 20 | 7.028243E-4 | 45.0 | 25 |
CGACGGT | 20 | 7.028243E-4 | 45.0 | 28 |
ATTACGG | 20 | 7.028243E-4 | 45.0 | 2 |
CCTAAGC | 20 | 7.028243E-4 | 45.0 | 16 |
GTAGACT | 20 | 7.028243E-4 | 45.0 | 16 |
TCTAAGT | 20 | 7.028243E-4 | 45.0 | 12 |
AGCGCGC | 20 | 7.028243E-4 | 45.0 | 20 |
TCGTCGG | 20 | 7.028243E-4 | 45.0 | 1 |
AACGATC | 20 | 7.028243E-4 | 45.0 | 22 |
CATAATC | 20 | 7.028243E-4 | 45.0 | 11 |
CACGTAG | 20 | 7.028243E-4 | 45.0 | 1 |
GACTAAT | 105 | 0.0 | 42.857143 | 9 |
CGTTTTT | 1965 | 0.0 | 41.335876 | 1 |
TGCACGG | 60 | 3.6379788E-12 | 41.250004 | 2 |
CATGTGC | 45 | 1.9233994E-8 | 40.0 | 37 |