FastQCFastQC Report
Sat 18 Jun 2016
SRR3550192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607066
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT31470.5183950344773056TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC26920.44344437013438404No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC25430.4189000866462625TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19840.3268178418821018No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC19000.3129807961572547TruSeq Adapter, Index 15 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA11190.18432921626314108No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC10490.17279834482576853No Hit
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC9920.1634089209410509TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC9190.15138386929921954No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG8490.139852997861847No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8310.13688791663509403No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7740.1274984927503764No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7620.1255217719325411No Hit
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC6920.11399090049516857TruSeq Adapter, Index 21 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6520.10740183110238424No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG6100.10048330823996073No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.0306525E-445.00000425
ACCGGTT207.0306525E-445.00000442
TTGTGCG207.0306525E-445.0000041
CGTTACG207.0306525E-445.00000445
TAGCACG207.0306525E-445.0000041
CGGTCTA207.0306525E-445.00000431
CGTATGG253.888624E-545.02
GATCGCA253.888624E-545.010
GTATAAG253.888624E-545.01
TCGTAAG302.1638025E-644.9999961
CGTTTTT17250.040.4347841
CGTTCGG1950.040.38461745
AACGGGA2250.040.0000044
CTACGAA1750.039.85714311
AGCTACG1750.039.8571439
ACGGGAC1250.039.6000025
GGATCGC403.4546247E-739.3750049
ACGTCAT403.4546247E-739.37500435
GCTACGA1800.038.7510
GCGAAAG356.243421E-638.5714261
Produced by FastQC (version 0.11.5)