##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	607066
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.107668688412794	33.0	31.0	34.0	30.0	34.0
2	32.233042206284	33.0	31.0	34.0	30.0	34.0
3	32.201427851337414	33.0	31.0	34.0	30.0	34.0
4	35.828669370381476	37.0	35.0	37.0	35.0	37.0
5	35.95104486167896	37.0	35.0	37.0	35.0	37.0
6	35.990719295760265	37.0	35.0	37.0	35.0	37.0
7	36.03008074904541	37.0	35.0	37.0	35.0	37.0
8	36.01960742324558	37.0	35.0	37.0	35.0	37.0
9	37.79821469164803	39.0	38.0	39.0	35.0	39.0
10	37.53664181489327	39.0	37.0	39.0	35.0	39.0
11	37.51700638810278	39.0	37.0	39.0	35.0	39.0
12	37.4668948681033	39.0	37.0	39.0	35.0	39.0
13	37.472208952568586	39.0	37.0	39.0	35.0	39.0
14	38.70861982057964	40.0	38.0	41.0	35.0	41.0
15	38.805602685704685	40.0	38.0	41.0	35.0	41.0
16	38.823006065238374	40.0	38.0	41.0	35.0	41.0
17	38.81420801033166	40.0	38.0	41.0	35.0	41.0
18	38.71777368523357	40.0	38.0	41.0	35.0	41.0
19	38.612897114975965	40.0	38.0	41.0	35.0	41.0
20	38.548212220747004	40.0	38.0	41.0	35.0	41.0
21	38.45897151215848	40.0	38.0	41.0	34.0	41.0
22	38.46833293249828	40.0	38.0	41.0	34.0	41.0
23	38.428223619837055	40.0	37.0	41.0	34.0	41.0
24	38.351055733643456	40.0	37.0	41.0	34.0	41.0
25	38.26777978012275	40.0	37.0	41.0	34.0	41.0
26	38.303716564591	40.0	37.0	41.0	34.0	41.0
27	38.24438034744163	40.0	37.0	41.0	34.0	41.0
28	38.18823982894776	40.0	37.0	41.0	34.0	41.0
29	38.13445490276181	40.0	37.0	41.0	34.0	41.0
30	38.02001265101323	40.0	37.0	41.0	34.0	41.0
31	37.95536070213124	40.0	37.0	41.0	34.0	41.0
32	37.7714762480521	40.0	36.0	41.0	33.0	41.0
33	37.6334154770651	40.0	36.0	41.0	33.0	41.0
34	37.57641508501547	40.0	36.0	41.0	33.0	41.0
35	37.436674101333296	40.0	36.0	41.0	33.0	41.0
36	37.320073929358585	40.0	36.0	41.0	33.0	41.0
37	37.23335189254546	40.0	35.0	41.0	33.0	41.0
38	37.12134759647221	40.0	35.0	41.0	32.0	41.0
39	37.073385760362136	40.0	35.0	41.0	32.0	41.0
40	36.952601529323005	39.0	35.0	41.0	32.0	41.0
41	36.83561095498677	39.0	35.0	41.0	31.0	41.0
42	36.77873740252296	39.0	35.0	41.0	31.0	41.0
43	36.697583129346725	39.0	35.0	41.0	31.0	41.0
44	36.62354340384736	39.0	35.0	41.0	31.0	41.0
45	36.53546401873931	39.0	35.0	41.0	31.0	41.0
46	36.432488395001535	39.0	35.0	41.0	31.0	41.0
47	36.35496634632808	39.0	35.0	41.0	31.0	41.0
48	36.28266778241575	39.0	35.0	41.0	31.0	41.0
49	36.227260297891824	39.0	35.0	41.0	30.0	41.0
50	36.13247324014193	39.0	35.0	41.0	30.0	41.0
51	35.11225962251221	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	18.0
10	20.0
11	22.0
12	19.0
13	20.0
14	25.0
15	25.0
16	49.0
17	88.0
18	128.0
19	261.0
20	465.0
21	732.0
22	1077.0
23	1608.0
24	2634.0
25	3762.0
26	4443.0
27	5327.0
28	5903.0
29	6628.0
30	7829.0
31	9757.0
32	12396.0
33	16757.0
34	30522.0
35	43188.0
36	42496.0
37	63863.0
38	118110.0
39	228611.0
40	277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60047507190322	20.640095146162032	24.190615188463855	12.568814593470892
2	32.54901443994557	25.646634797534368	24.610997815723497	17.19335294679656
3	31.272711698563256	25.064819970151515	26.886532930521557	16.77593540076367
4	28.82964949445365	26.950117450161926	25.780722359677533	18.439510695706893
5	24.089637700019438	31.34024965983929	24.788573235859033	19.781539404282235
6	24.010568867306027	35.29879782428929	25.279129452151828	15.411503856252862
7	74.50886724013533	8.863286693703815	10.713991559402109	5.913854506758738
8	74.70192697334393	7.908201085219729	10.980684143075054	6.409187798361299
9	67.19055259230463	10.666056079569602	13.717454115368016	8.425937212757756
10	35.98686139563079	27.07020323984542	20.449341587240927	16.493593777282868
11	28.114076558397276	26.297634853541464	25.425406792671634	20.162881795389627
12	24.480040061541906	23.92985276724442	31.238283810985955	20.35182336022772
13	23.23668266712351	25.202696247195526	31.645982479664486	19.91463860601648
14	19.47465349731331	28.795551060345993	29.72016222288845	22.00963321945225
15	18.976190397749175	26.270619669031042	33.51134802476172	21.24184190845806
16	20.209828914813215	27.137411747651818	30.352383431126107	22.30037590640886
17	21.0741500924117	26.259912431267768	28.034842998949046	24.631094477371487
18	21.764849291510313	26.200940260202348	30.403613445655004	21.63059700263233
19	22.90887646483249	27.119950713760943	27.770621316298396	22.200551505108177
20	23.54439220776654	27.426342440525413	28.277485479338328	20.751779872369728
21	22.447641607337587	28.276497118929406	28.68320742719902	20.592653846533985
22	21.042357832591513	26.79922776106717	26.687378308124654	25.471036098216672
23	20.59973709613123	27.514306516919085	27.799613221626647	24.086343165323047
24	21.364563325898665	26.812570626587558	28.30631265793176	23.516553389582022
25	21.391907963878722	28.02084122648938	26.446053641613926	24.141197168017975
26	20.763475470541916	28.68501942128204	27.261780432440624	23.289724675735425
27	22.51369702800025	27.427330800934328	27.965987223794446	22.09298494727097
28	19.475312404252584	28.185732688043803	28.936886598821214	23.402068308882395
29	21.836011240952384	28.09562716409748	26.91667792299355	23.15168367195659
30	23.04345820718011	29.15449061551792	25.82717529889666	21.974875878405314
31	23.229764144261086	29.661190051823034	25.305979909927423	21.803065893988464
32	23.346720125983005	29.726092385341957	25.167774179413772	21.759413309261266
33	22.9383625503652	29.477025562294713	24.974055539265912	22.61055634807418
34	22.643007514833645	27.67540926357266	27.26112152550135	22.420461696092353
35	23.61835451170054	27.308562825129396	26.484434970826896	22.588647692343173
36	23.98305950259115	29.911904142218475	25.782040173556087	20.322996181634288
37	23.06289596188882	29.421018472456044	28.263483706878663	19.252601858776476
38	21.30822678259036	30.577894331094146	27.044670595948382	21.06920829036711
39	25.013095775418158	27.595846250654787	26.36418445440858	21.02687351951847
40	25.14635970388722	25.759143157416165	28.265625154431312	20.8288719842653
41	23.238659387941343	25.678427057354554	27.14531863092316	23.93759492378094
42	24.035936784468245	25.43710239084383	28.27106113668036	22.255899688007563
43	24.00941578016229	24.679853590878093	28.973950114155627	22.33678051480399
44	22.306800249066825	25.825198578078822	28.012275436278756	23.855725736575593
45	22.909205918302128	25.235312140689807	27.466371037086578	24.389110903921484
46	23.68951646114261	26.191221382847996	28.397406542287	21.721855613722397
47	22.167111977939797	25.705606968599792	30.35996086092781	21.76732019253261
48	21.400144300619704	24.994316927648725	30.10381078828332	23.50172798344826
49	23.439955457890903	24.06278724224384	29.58245067257926	22.914806627285994
50	21.793017563164465	23.976799886668008	29.689358323477183	24.540824226690344
51	21.655800193059733	23.27391090919274	27.633733399663296	27.436555498084232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	993.0
1	1244.5
2	1496.0
3	2677.0
4	3858.0
5	2831.0
6	1804.0
7	1903.5
8	2003.0
9	2088.5
10	2174.0
11	2245.0
12	2316.0
13	2339.0
14	2362.0
15	2455.0
16	2548.0
17	2316.0
18	2084.0
19	2118.5
20	2153.0
21	2308.5
22	2464.0
23	2666.0
24	2868.0
25	3782.0
26	5424.0
27	6152.0
28	7069.0
29	7986.0
30	9035.5
31	10085.0
32	11131.5
33	12178.0
34	13374.0
35	14570.0
36	15631.0
37	16692.0
38	18552.0
39	20412.0
40	24687.0
41	28962.0
42	33246.0
43	37530.0
44	44879.5
45	52229.0
46	59124.5
47	66020.0
48	63807.0
49	61594.0
50	52529.0
51	43464.0
52	38319.5
53	33175.0
54	30761.0
55	28347.0
56	26859.0
57	25371.0
58	23860.0
59	22349.0
60	22182.5
61	22016.0
62	20058.0
63	18100.0
64	16093.0
65	14086.0
66	11949.5
67	9813.0
68	8606.0
69	7399.0
70	6362.5
71	5326.0
72	4627.5
73	3929.0
74	3171.5
75	1882.0
76	1350.0
77	1129.5
78	909.0
79	678.0
80	447.0
81	321.5
82	196.0
83	123.0
84	50.0
85	51.5
86	53.0
87	41.5
88	30.0
89	16.5
90	3.0
91	2.5
92	2.0
93	2.5
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	607066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.58274643724237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58835783329447	26.18475877515175
2	9.812969097641561	6.983447823957493
3	3.436983632310029	3.6689195129232006
4	1.9469386253292083	2.7710969373584975
5	1.3027071162022126	2.3176948498907284
6	1.0311416226733516	2.201451054028351
7	0.8512963932221929	2.1204024592074986
8	0.7336969995456397	2.0885563437278423
9	0.6533766481176856	2.0924042038188246
>10	6.5078535597777005	40.98494241900431
>50	0.08330627127089768	2.0530835174923427
>100	0.04304157348830515	2.8048542928457247
>500	0.005090938799692007	1.3513758998997194
>1k	0.0032396883270767317	2.3770119106937058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	3147	0.5183950344773056	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	2692	0.44344437013438404	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	2543	0.4189000866462625	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1984	0.3268178418821018	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1900	0.3129807961572547	TruSeq Adapter, Index 15 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1119	0.18432921626314108	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	1049	0.17279834482576853	No Hit
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	992	0.1634089209410509	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC	919	0.15138386929921954	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	849	0.139852997861847	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	831	0.13688791663509403	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	774	0.1274984927503764	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	762	0.1255217719325411	No Hit
ACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	692	0.11399090049516857	TruSeq Adapter, Index 21 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	652	0.10740183110238424	No Hit
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	610	0.10048330823996073	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5365149753074625	0.0
2	0.0	0.0	0.0	1.5899424444788541	0.0
3	0.0	0.0	0.0	2.202231717803336	0.0
4	0.0	0.0	0.0	2.839230001350759	0.0
5	0.0	0.0	0.0	4.531961928357049	0.0
6	0.0	0.0	0.0	5.616687477144166	0.0
7	0.0	0.0	0.0	6.661549156104938	0.0
8	0.0	0.0	0.0	8.50385295832743	0.0
9	0.0	0.0	0.0	9.375586838992795	0.0
10	0.0	0.0	0.0	10.93143084936399	0.0
11	0.0	0.0	0.0	13.016541858710585	0.0
12	0.0	0.0	0.0	14.5809516592924	0.0
13	0.0	0.0	0.0	15.243482586736862	0.0
14	0.0	0.0	0.0	15.532083826140815	0.0
15	0.0	0.0	0.0	16.010450264056956	0.0
16	0.0	0.0	0.0	16.922706921487944	0.0
17	0.0	0.0	0.0	17.993595424550215	0.0
18	0.0	0.0	0.0	19.22558667426606	0.0
19	0.0	0.0	0.0	19.89668339192114	0.0
20	0.0	0.0	0.0	20.544718366701478	0.0
21	0.0	0.0	0.0	21.313498038104587	0.0
22	0.0	0.0	0.0	22.083924976855894	0.0
23	0.0	0.0	0.0	22.80740479618361	0.0
24	0.0	0.0	0.0	23.386583995809353	0.0
25	0.0	0.0	0.0	23.896083786606397	0.0
26	1.6472673481960774E-4	0.0	0.0	24.393393799026793	0.0
27	1.6472673481960774E-4	0.0	0.0	24.87406641123041	0.0
28	1.6472673481960774E-4	0.0	0.0	25.38208366141408	0.0
29	1.6472673481960774E-4	0.0	0.0	25.887300557105817	0.0
30	1.6472673481960774E-4	0.0	0.0	26.46203213489143	0.0
31	1.6472673481960774E-4	0.0	0.0	26.976638454467885	0.0
32	1.6472673481960774E-4	0.0	0.0	27.4648884964732	0.0
33	1.6472673481960774E-4	0.0	0.0	27.9529738117437	0.0
34	1.6472673481960774E-4	0.0	0.0	28.453743085595306	0.0
35	1.6472673481960774E-4	0.0	0.0	28.944793482092557	0.0
36	1.6472673481960774E-4	0.0	0.0	29.421018472456044	0.0
37	1.6472673481960774E-4	0.0	0.0	29.8667690168779	0.0
38	3.294534696392155E-4	0.0	0.0	30.323062072328216	0.0
39	3.294534696392155E-4	0.0	0.0	30.77919040104371	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.0306525E-4	45.000004	25
ACCGGTT	20	7.0306525E-4	45.000004	42
TTGTGCG	20	7.0306525E-4	45.000004	1
CGTTACG	20	7.0306525E-4	45.000004	45
TAGCACG	20	7.0306525E-4	45.000004	1
CGGTCTA	20	7.0306525E-4	45.000004	31
CGTATGG	25	3.888624E-5	45.0	2
GATCGCA	25	3.888624E-5	45.0	10
GTATAAG	25	3.888624E-5	45.0	1
TCGTAAG	30	2.1638025E-6	44.999996	1
CGTTTTT	1725	0.0	40.434784	1
CGTTCGG	195	0.0	40.384617	45
AACGGGA	225	0.0	40.000004	4
CTACGAA	175	0.0	39.857143	11
AGCTACG	175	0.0	39.857143	9
ACGGGAC	125	0.0	39.600002	5
GGATCGC	40	3.4546247E-7	39.375004	9
ACGTCAT	40	3.4546247E-7	39.375004	35
GCTACGA	180	0.0	38.75	10
GCGAAAG	35	6.243421E-6	38.571426	1
>>END_MODULE