FastQCFastQC Report
Sat 18 Jun 2016
SRR3550191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353884
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.5657221010274553No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT13770.3891105560014016No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC10020.28314362898576934No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC9620.2718404901041019No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC6940.19610945959693007No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6270.1771767019701371No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5010.14157181449288467No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4960.14015892213267625No Hit
TCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC4660.13168156797142566No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4650.131398989499384No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC4220.11924811520159148No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4160.11755264436934137No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3780.1068146624317573No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG3550.10031535757479852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT453.8380676E-1045.00000414
AATAAGG351.2087366E-745.0000042
TCACGAC207.026662E-445.025
AGGTAAT253.8853148E-545.06
CCTACCG207.026662E-445.033
ATTCGCG207.026662E-445.01
TGCGACG207.026662E-445.01
TTAACGG207.026662E-445.02
CGTAAGG551.8189894E-1245.02
TAGATAG207.026662E-445.01
TTACGCG207.026662E-445.01
TAGTGTT207.026662E-445.011
GTGCGCC207.026662E-445.045
AGTAACG253.8853148E-545.01
TACAACG207.026662E-445.01
TACCGGG253.8853148E-545.03
ATGATCG302.1612286E-644.99999627
CGTGAGT302.1612286E-644.99999632
GCGTACG302.1612286E-644.9999961
TATAGGG1400.043.392863