##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550191_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 353884 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.169024313051736 33.0 31.0 34.0 30.0 34.0 2 32.308075527574005 34.0 31.0 34.0 30.0 34.0 3 32.279922234404495 34.0 31.0 34.0 30.0 34.0 4 35.867027048411344 37.0 35.0 37.0 35.0 37.0 5 35.98467011789174 37.0 35.0 37.0 35.0 37.0 6 36.03741621548304 37.0 35.0 37.0 35.0 37.0 7 36.05939234325372 37.0 35.0 37.0 35.0 37.0 8 36.04875608956607 37.0 36.0 37.0 35.0 37.0 9 37.85433927501667 39.0 38.0 39.0 35.0 39.0 10 37.59024708661595 39.0 37.0 39.0 35.0 39.0 11 37.565445174124854 39.0 37.0 39.0 35.0 39.0 12 37.42049089532163 39.0 37.0 39.0 35.0 39.0 13 37.37597348283619 39.0 37.0 39.0 35.0 39.0 14 38.57457528455652 40.0 38.0 41.0 35.0 41.0 15 38.70130325191305 40.0 38.0 41.0 35.0 41.0 16 38.73778130686892 40.0 38.0 41.0 35.0 41.0 17 38.71659357303523 40.0 38.0 41.0 35.0 41.0 18 38.5966616179313 40.0 38.0 41.0 35.0 41.0 19 38.45762170654791 40.0 37.0 41.0 35.0 41.0 20 38.34198777000373 40.0 37.0 41.0 34.0 41.0 21 38.24699053927276 40.0 36.0 41.0 34.0 41.0 22 38.229903019068395 40.0 36.0 41.0 34.0 41.0 23 38.21310938047496 40.0 36.0 41.0 34.0 41.0 24 38.13380373229646 40.0 36.0 41.0 34.0 41.0 25 38.065897299680124 40.0 36.0 41.0 34.0 41.0 26 38.0942540493495 40.0 36.0 41.0 34.0 41.0 27 38.0435566456805 40.0 36.0 41.0 34.0 41.0 28 37.973779543579255 40.0 36.0 41.0 34.0 41.0 29 37.92724169501871 40.0 36.0 41.0 34.0 41.0 30 37.79565620372777 40.0 36.0 41.0 34.0 41.0 31 37.67469848877033 40.0 35.0 41.0 33.0 41.0 32 37.446403341207855 40.0 35.0 41.0 33.0 41.0 33 37.26092165794441 40.0 35.0 41.0 33.0 41.0 34 37.109894767777014 40.0 35.0 41.0 33.0 41.0 35 36.97955262176306 40.0 35.0 41.0 32.0 41.0 36 36.865260932961085 40.0 35.0 41.0 32.0 41.0 37 36.73704942862633 40.0 35.0 41.0 31.0 41.0 38 36.637152287190155 39.0 35.0 41.0 31.0 41.0 39 36.560370064766985 39.0 35.0 41.0 31.0 41.0 40 36.43946603971923 39.0 35.0 41.0 31.0 41.0 41 36.33209187191283 39.0 35.0 41.0 30.0 41.0 42 36.257714392286736 39.0 35.0 41.0 30.0 41.0 43 36.112808151823764 39.0 35.0 41.0 30.0 41.0 44 36.01232324716574 39.0 35.0 41.0 30.0 41.0 45 35.92834375105967 39.0 35.0 41.0 29.0 41.0 46 35.8489872387562 39.0 35.0 41.0 29.0 41.0 47 35.787746832295326 39.0 35.0 41.0 29.0 41.0 48 35.737487425257996 38.0 35.0 41.0 29.0 41.0 49 35.68438810457664 38.0 35.0 41.0 29.0 41.0 50 35.57191904691933 38.0 35.0 41.0 28.0 41.0 51 34.617725017237284 37.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 7.0 10 10.0 11 14.0 12 15.0 13 12.0 14 17.0 15 13.0 16 35.0 17 65.0 18 113.0 19 212.0 20 372.0 21 535.0 22 810.0 23 1298.0 24 2196.0 25 3046.0 26 3574.0 27 4073.0 28 4122.0 29 4335.0 30 4797.0 31 5822.0 32 7177.0 33 10104.0 34 19014.0 35 30152.0 36 23585.0 37 35109.0 38 63228.0 39 129821.0 40 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.947553435589064 21.288331769732455 25.81269568559189 12.951419109086594 2 31.806750234540132 26.115619807620575 25.272405647048185 16.805224310791107 3 32.039312317030436 24.616541013439434 26.77346249053362 16.57068417899651 4 27.23576087079382 27.31657831379774 27.364051497100743 18.083609318307694 5 23.141763967853873 32.05965796701744 25.52616111494162 19.272416950187065 6 24.075968396423686 36.596172757174664 24.71516090018198 14.612697946219665 7 75.7341388703643 9.270834510743633 10.175085621277029 4.819940997615038 8 76.80821964259475 8.36347503701778 10.027297080399226 4.801008239988245 9 70.29930711758655 10.859490680561992 12.281425551875756 6.559776649975698 10 37.33228967684326 29.39748618191272 18.311932723717376 14.958291417526649 11 30.19916130709498 26.297882922087464 25.15061432559822 18.352341445219338 12 26.6689084558782 24.22403951577353 30.008420838466844 19.09863118988143 13 23.507420510675814 27.72546936284206 29.904997117699583 18.86211300878254 14 18.59253314645477 30.38566309864249 30.480044308304414 20.54175944659832 15 19.209967107865854 25.76917860089747 34.69413706186208 20.326717229374598 16 20.861355698477468 26.303251913056258 31.56345017011224 21.271942218354038 17 20.6426399611172 26.21028359575454 28.592702693537998 24.554373749590262 18 21.847554565902954 25.445061093465654 30.790598049078227 21.916786291553166 19 23.46136021973302 27.16200788959094 28.57546540674345 20.801166483932587 20 23.857535237535462 27.449390195657337 28.691605158752587 20.001469408054618 21 22.95243639158594 28.807744910761716 28.53816504843395 19.701653649218386 22 21.046162019192728 27.022131545930307 27.759378779487065 24.172327655389903 23 20.794102022131547 27.8260672988889 27.732251246171064 23.64757943280849 24 19.940997615037695 27.18574448124244 29.50627889364877 23.366979010071095 25 19.273547264075233 29.38137920900634 28.188050321574305 23.157023205344125 26 18.881893501825456 30.11410518701043 28.33188276384352 22.67211854732059 27 21.501113359179843 28.7777915927253 28.204722451424765 21.516372596670095 28 18.165557075199786 28.947056097478267 29.619875439409526 23.267511387912425 29 20.03707429553187 29.259305309084333 28.33894722564456 22.364673169739234 30 22.027557052593504 29.173684032055704 26.711577805156494 22.0871811101943 31 23.83012512574742 29.168597619558952 24.050818912411977 22.950458342281653 32 22.97673814018153 29.92449503227046 24.318703303907498 22.780063523640514 33 23.85866555142363 29.64304687411694 23.75665472301658 22.741632851442844 34 21.125849148308482 28.872455380859265 25.369895219902567 24.631800250929683 35 22.78599767155339 28.880932735020515 25.168416769336844 23.16465282408925 36 24.54080998293226 29.406528693018053 24.24749352895299 21.805167795096697 37 23.568457460636818 30.04261283358389 24.928225068101412 21.460704637677882 38 21.592103627177266 30.656938431802512 24.306835008081745 23.444122932938477 39 23.729809768172622 28.92247177041064 25.34050705881023 22.007211402606504 40 24.65468910716506 27.627979789987677 26.011065772965154 21.706265329882108 41 22.357891286410236 26.089905166664785 25.649647907223837 25.90255563970115 42 23.61056165297103 25.652191113472213 26.598828994811857 24.1384182387449 43 24.25116704908953 24.57104587944072 27.600287099727595 23.577499971742153 44 22.264357812164437 26.61917464479886 27.038803675780766 24.077663867255936 45 22.18919193860135 27.02778311537114 26.26679929016288 24.516225655864634 46 23.19319324976546 27.335511071424534 26.83054334188604 22.640752336923963 47 22.19625640040239 26.448497247685683 28.153575747985215 23.20167060392671 48 21.23181607532412 25.572221405884417 29.028156118954236 24.167806399837236 49 23.39721490657956 24.479490454499214 28.704886346938547 23.418408291982683 50 21.179821636468446 25.542268087847997 28.84984910309593 24.42806117258763 51 21.57288829107843 24.896858857704785 26.66156141560511 26.86869143561167 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 830.0 1 975.5 2 1121.0 3 2508.0 4 3895.0 5 2773.0 6 1651.0 7 1685.0 8 1719.0 9 1814.5 10 1910.0 11 1946.5 12 1983.0 13 1987.0 14 1991.0 15 1881.0 16 1771.0 17 1768.0 18 1765.0 19 1759.0 20 1753.0 21 1664.5 22 1576.0 23 1674.0 24 1772.0 25 2343.0 26 3319.5 27 3725.0 28 4592.0 29 5459.0 30 6049.0 31 6639.0 32 6956.0 33 7273.0 34 7869.5 35 8466.0 36 8821.5 37 9177.0 38 10428.0 39 11679.0 40 12513.0 41 13347.0 42 15504.0 43 17661.0 44 20192.0 45 22723.0 46 23275.0 47 23827.0 48 25972.0 49 28117.0 50 30586.0 51 33055.0 52 31524.0 53 29993.0 54 26610.5 55 23228.0 56 20726.5 57 18225.0 58 16927.0 59 15629.0 60 14185.0 61 12741.0 62 11388.0 63 10035.0 64 9186.0 65 8337.0 66 7140.5 67 5944.0 68 5065.0 69 4186.0 70 3657.0 71 3128.0 72 2526.5 73 1925.0 74 1665.0 75 1031.5 76 658.0 77 480.0 78 302.0 79 219.0 80 136.0 81 130.5 82 125.0 83 103.5 84 82.0 85 42.0 86 2.0 87 2.5 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 353884.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.75028010756529 #Duplication Level Percentage of deduplicated Percentage of total 1 75.71493895540249 25.55400398072041 2 8.845557115064423 5.9708006068178285 3 3.069175565964347 3.107566051517758 4 1.5934799623428746 2.1512158029945843 5 1.0406217114458403 1.7560637123655545 6 0.7819070529297302 1.5833749232675565 7 0.7248123762771205 1.7123834507347977 8 0.5536688886919416 1.4949184064157937 9 0.5259840197494428 1.597689719878216 >10 7.01982649677838 48.73587260413602 >50 0.09060005405270166 1.9385741205891347 >100 0.033555575575074686 2.3689279490242865 >500 0.003355557557507469 0.7882270120812255 >1k 0.0025166681681306016 1.2403816594568422 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2002 0.5657221010274553 No Hit CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT 1377 0.3891105560014016 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC 1002 0.28314362898576934 No Hit GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC 962 0.2718404901041019 No Hit CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC 694 0.19610945959693007 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 627 0.1771767019701371 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 501 0.14157181449288467 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 496 0.14015892213267625 No Hit TCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC 466 0.13168156797142566 No Hit GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 465 0.131398989499384 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC 422 0.11924811520159148 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 416 0.11755264436934137 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 378 0.1068146624317573 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG 355 0.10031535757479852 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.4012614302991941 0.0 2 0.0 0.0 0.0 1.107707610403409 0.0 3 0.0 0.0 0.0 1.4688428976726837 0.0 4 0.0 0.0 0.0 1.870669484915961 0.0 5 0.0 0.0 0.0 2.9204485085508245 0.0 6 0.0 0.0 0.0 3.621243119214206 0.0 7 0.0 0.0 0.0 4.299714030586294 0.0 8 0.0 0.0 0.0 5.5509715047868795 0.0 9 0.0 0.0 0.0 6.178578291191464 0.0 10 0.0 0.0 0.0 7.447638209130676 0.0 11 0.0 0.0 0.0 9.176453301081711 0.0 12 0.0 0.0 0.0 10.579737993240723 0.0 13 0.0 0.0 0.0 11.11381130539951 0.0 14 0.0 0.0 0.0 11.334787670536107 0.0 15 0.0 0.0 0.0 11.698183585581717 0.0 16 0.0 0.0 0.0 12.436843711498684 0.0 17 0.0 0.0 0.0 13.275819194990449 0.0 18 2.82578472041686E-4 0.0 0.0 14.289145595731934 0.0 19 2.82578472041686E-4 0.0 0.0 14.824914378722971 0.0 20 2.82578472041686E-4 0.0 0.0 15.358139955465633 0.0 21 2.82578472041686E-4 0.0 0.0 15.923579478021047 0.0 22 2.82578472041686E-4 0.0 0.0 16.46584756586904 0.0 23 2.82578472041686E-4 0.0 0.0 16.995682200947204 0.0 24 2.82578472041686E-4 0.0 0.0 17.46787082772886 0.0 25 2.82578472041686E-4 0.0 0.0 17.87676187677318 0.0 26 2.82578472041686E-4 0.0 0.0 18.243548733483287 0.0 27 2.82578472041686E-4 0.0 0.0 18.61259621796973 0.0 28 2.82578472041686E-4 0.0 0.0 18.992099105921714 0.0 29 2.82578472041686E-4 0.0 0.0 19.391099908444573 0.0 30 2.82578472041686E-4 0.0 0.0 19.85791954425744 0.0 31 2.82578472041686E-4 0.0 0.0 20.232336019712672 0.0 32 2.82578472041686E-4 0.0 0.0 20.63896644098066 0.0 33 5.65156944083372E-4 0.0 0.0 21.006883611578935 0.0 34 5.65156944083372E-4 0.0 0.0 21.376213674537418 0.0 35 5.65156944083372E-4 0.0 0.0 21.76645454442699 0.0 36 5.65156944083372E-4 0.0 0.0 22.095941042827594 0.0 37 5.65156944083372E-4 0.0 0.0 22.46753173356241 0.0 38 5.65156944083372E-4 0.0 0.0 22.84138305207356 0.0 39 5.65156944083372E-4 0.0 0.0 23.225972352522295 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATAT 45 3.8380676E-10 45.000004 14 AATAAGG 35 1.2087366E-7 45.000004 2 TCACGAC 20 7.026662E-4 45.0 25 AGGTAAT 25 3.8853148E-5 45.0 6 CCTACCG 20 7.026662E-4 45.0 33 ATTCGCG 20 7.026662E-4 45.0 1 TGCGACG 20 7.026662E-4 45.0 1 TTAACGG 20 7.026662E-4 45.0 2 CGTAAGG 55 1.8189894E-12 45.0 2 TAGATAG 20 7.026662E-4 45.0 1 TTACGCG 20 7.026662E-4 45.0 1 TAGTGTT 20 7.026662E-4 45.0 11 GTGCGCC 20 7.026662E-4 45.0 45 AGTAACG 25 3.8853148E-5 45.0 1 TACAACG 20 7.026662E-4 45.0 1 TACCGGG 25 3.8853148E-5 45.0 3 ATGATCG 30 2.1612286E-6 44.999996 27 CGTGAGT 30 2.1612286E-6 44.999996 32 GCGTACG 30 2.1612286E-6 44.999996 1 TATAGGG 140 0.0 43.39286 3 >>END_MODULE