FastQCFastQC Report
Sat 18 Jun 2016
SRR3550190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637416
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58040.9105513510799854No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC37620.5901954139839602No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT31770.4984186151587033Illumina PCR Primer Index 5 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC31700.49732043124113606Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTC16020.25132723370608834No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC15300.24003162769682596Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGC14810.2323443402738557No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG13630.213832097092009No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC12840.2014383071651794Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT12620.19798687199568255No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT11140.17476812630997654No Hit
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC7840.12299659876752389Illumina PCR Primer Index 5 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC6740.10573942292003967No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC6470.10150357066656626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCGG406.8066583E-945.0000042
CACCGGC406.8066583E-945.00000419
CCCGGTC207.0309185E-445.00000430
GTCGCTC253.8888436E-545.022
CGGCGCA502.1827873E-1145.022
TATCGAG253.8888436E-545.01
AGTCGAG253.8888436E-545.01
TAATCGG253.8888436E-545.02
CGTTTTT51400.043.774321
CGAATAT1100.042.95454814
CTACGAA1150.041.08695611
CGGGTCC451.925946E-839.9999966
ACTACGG900.039.9999962
TTCACGG403.4549885E-739.3750042
ATATGCG403.4549885E-739.3750041
TACGAAT1200.039.37499612
GCTACGA1200.039.37499610
ATTGGGC1500.039.04
CGTGTAT750.039.022
AGCTACG1250.037.89
Produced by FastQC (version 0.11.5)