##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637416
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88542333421188	33.0	31.0	34.0	30.0	34.0
2	32.02561906196267	33.0	31.0	34.0	30.0	34.0
3	31.940923980571558	33.0	31.0	34.0	30.0	34.0
4	35.62583618861152	37.0	35.0	37.0	33.0	37.0
5	35.8037859106141	37.0	35.0	37.0	35.0	37.0
6	35.87093044416833	37.0	35.0	37.0	35.0	37.0
7	36.00935338930934	37.0	35.0	37.0	35.0	37.0
8	35.99729219222611	37.0	35.0	37.0	35.0	37.0
9	37.798012600876035	39.0	38.0	39.0	35.0	39.0
10	37.36255443854563	39.0	37.0	39.0	34.0	39.0
11	37.37379042885651	39.0	37.0	39.0	34.0	39.0
12	37.38282691366392	39.0	37.0	39.0	35.0	39.0
13	37.40101597700717	39.0	37.0	39.0	35.0	39.0
14	38.58818887508315	40.0	38.0	41.0	34.0	41.0
15	38.70085156318636	40.0	38.0	41.0	35.0	41.0
16	38.7370273102652	40.0	38.0	41.0	35.0	41.0
17	38.72041806292908	40.0	38.0	41.0	35.0	41.0
18	38.56338874455614	40.0	38.0	41.0	35.0	41.0
19	38.37167250272977	40.0	37.0	41.0	34.0	41.0
20	38.2150416682355	40.0	37.0	41.0	34.0	41.0
21	38.12946803971033	40.0	36.0	41.0	34.0	41.0
22	38.153035066581324	40.0	36.0	41.0	34.0	41.0
23	38.11525753981701	40.0	36.0	41.0	34.0	41.0
24	38.00277997414561	40.0	36.0	41.0	34.0	41.0
25	37.93279585074739	40.0	36.0	41.0	34.0	41.0
26	37.99799816760169	40.0	36.0	41.0	34.0	41.0
27	37.971964933418676	40.0	36.0	41.0	34.0	41.0
28	37.92172145035581	40.0	36.0	41.0	34.0	41.0
29	37.865908605996715	40.0	36.0	41.0	34.0	41.0
30	37.70967468654693	40.0	36.0	41.0	33.0	41.0
31	37.558285327007795	40.0	36.0	41.0	33.0	41.0
32	37.30343292292631	40.0	36.0	41.0	33.0	41.0
33	37.023581146378504	40.0	35.0	41.0	31.0	41.0
34	36.76935784479837	40.0	35.0	41.0	31.0	41.0
35	36.597076320644604	40.0	35.0	41.0	30.0	41.0
36	36.48070333973418	40.0	35.0	41.0	30.0	41.0
37	36.391383335215934	40.0	35.0	41.0	30.0	41.0
38	36.334541335642655	40.0	35.0	41.0	30.0	41.0
39	36.24431925147784	40.0	35.0	41.0	29.0	41.0
40	36.14293491220804	39.0	35.0	41.0	29.0	41.0
41	36.03222699147809	39.0	35.0	41.0	28.0	41.0
42	36.01978927419456	39.0	35.0	41.0	28.0	41.0
43	35.923458149779734	39.0	35.0	41.0	27.0	41.0
44	35.825536227518604	39.0	35.0	41.0	27.0	41.0
45	35.73348174504562	39.0	35.0	41.0	26.0	41.0
46	35.61175119545164	39.0	35.0	41.0	26.0	41.0
47	35.55308307290686	39.0	35.0	41.0	26.0	41.0
48	35.50868035945128	39.0	35.0	41.0	26.0	41.0
49	35.44480527630307	39.0	35.0	41.0	26.0	41.0
50	35.32840876288013	39.0	35.0	41.0	24.0	41.0
51	34.36487474428003	38.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	13.0
10	14.0
11	18.0
12	14.0
13	11.0
14	17.0
15	20.0
16	44.0
17	91.0
18	203.0
19	323.0
20	602.0
21	947.0
22	1569.0
23	2506.0
24	4320.0
25	7274.0
26	10070.0
27	10150.0
28	9365.0
29	8966.0
30	9327.0
31	11224.0
32	13598.0
33	18216.0
34	30265.0
35	43023.0
36	42680.0
37	64001.0
38	120478.0
39	227908.0
40	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.0693048182035	20.77560023595266	25.236109542276942	14.9189854035669
2	35.8775117035029	23.51368650928122	24.89865331275023	15.710148474465655
3	28.174065288602733	23.25780965648807	33.22461312549419	15.343511929415014
4	24.501581384841298	25.24191422869837	32.92151436424564	17.33499002221469
5	21.532562721990033	29.012450267956876	31.443201927783427	18.011785082269665
6	21.753611456254628	33.19276579188473	31.06166145813723	13.99196129372341
7	72.44452602382118	6.573415163723534	15.52330032506244	5.458758487392848
8	73.06656877141458	5.780683258656827	14.96181457635202	6.190933393576565
9	67.3613464362363	7.889667030636193	17.70303851801649	7.04594801511101
10	33.97969301053001	24.75275173513059	25.556779246206556	15.710776008132838
11	25.34686923453443	25.904432897824968	29.556521957403014	19.192175910237587
12	22.569248340173452	22.63011910589003	35.356501876325666	19.444130677610854
13	21.692113156870864	25.06730298580519	36.147037413557236	17.09354644376671
14	18.76608055022152	28.126058963063365	33.72428680798725	19.383573678727863
15	17.225171630457975	26.793804987637586	37.30844534809293	18.67257803381151
16	19.078435433060985	26.582169258380713	34.70496504637474	19.634430262183567
17	19.50045182424037	26.64915847735231	31.71539465592329	22.13499504248403
18	19.91132949282729	26.19560851939707	34.62417008672515	19.26889190105049
19	19.900190770234826	29.413130514452103	31.764812932213815	18.92186578309926
20	20.08531320205329	28.82450393463609	31.76983320155126	19.320349661759355
21	19.06023695671273	30.173230668825386	32.041712162857536	18.724820211604353
22	17.942756378879725	27.604578485635756	30.01901427011559	24.43365086536893
23	18.195966213587358	28.454729721249546	30.581755086160374	22.767548979002726
24	18.683402989570393	27.207506557726823	31.874317557136937	22.234772895565847
25	18.290880680748522	28.861057770749397	30.133225397542578	22.7148361509595
26	18.123172308194334	28.493793692031577	32.052850885450006	21.330183114324083
27	20.19497471039321	27.70906284122143	30.711968322100482	21.383994126284875
28	17.703352284850084	27.843041279164627	31.954641866536136	22.49896456944915
29	20.587183252381493	25.521480477427616	30.647489237797608	23.24384703239329
30	21.91410319163623	27.101296484556396	28.488459655860538	22.496140667946836
31	19.719931724337012	30.18703640950337	27.40110069405224	22.69193117210738
32	20.665624960779148	28.512933468880608	29.477923365588566	21.343518204751685
33	21.077600813283635	26.936882663754915	28.950167551489137	23.03534897147232
34	19.760250762453406	28.670758186176688	29.120072291878458	22.448918759491445
35	20.341190054846443	25.630043801849965	30.627408160447807	23.40135798285578
36	21.12513648857261	28.32200635064071	29.345984412063707	21.20687274872297
37	18.488553785910614	30.19174291200723	31.195483012663626	20.124220289418528
38	18.88311557915082	28.797363103530504	31.957936418288842	20.361584899029832
39	21.85291865908606	26.476429835460674	31.759165129209183	19.911486376244085
40	21.699643560877043	25.905531081742534	32.216480289167514	20.17834506821291
41	18.763884182386384	26.465918646535386	31.111707268094936	23.658489902983295
42	20.26870991628701	25.755393651869422	32.11905568733763	21.85684074450594
43	19.70989118566211	25.16990474038932	32.68964067422217	22.430563399726395
44	20.19136639180692	26.1476021938577	29.449370583731817	24.211660830603563
45	21.13094117499404	24.676977044818454	29.92127590145211	24.270805878735395
46	22.165273541925522	26.057551112617194	29.898370922599998	21.878804422857286
47	18.960615987047706	25.97597173588363	32.66783387928762	22.39557839778104
48	18.479925197986873	24.431768264367385	32.68273780388318	24.405568733762568
49	20.847295957428116	23.1062602758638	32.92151436424564	23.124929402462442
50	19.268107483966514	23.05872460057482	32.20549844999184	25.467669465466823
51	18.990423836238815	23.1831331500935	29.827302734791722	27.999140278875963
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	823.0
1	950.5
2	1078.0
3	5362.0
4	9646.0
5	6829.0
6	4012.0
7	4077.5
8	4143.0
9	4303.0
10	4463.0
11	4576.5
12	4690.0
13	4788.0
14	4886.0
15	4806.0
16	4726.0
17	4592.5
18	4459.0
19	4252.5
20	4046.0
21	4068.0
22	4090.0
23	4001.5
24	3913.0
25	4460.0
26	5154.5
27	5302.0
28	6095.0
29	6888.0
30	7786.5
31	8685.0
32	9870.0
33	11055.0
34	13003.0
35	14951.0
36	16724.0
37	18497.0
38	21545.5
39	24594.0
40	29711.5
41	34829.0
42	40106.5
43	45384.0
44	56277.5
45	67171.0
46	73875.5
47	80580.0
48	76805.0
49	73030.0
50	61616.0
51	50202.0
52	43516.0
53	36830.0
54	31181.5
55	25533.0
56	22508.5
57	19484.0
58	17381.0
59	15278.0
60	14264.0
61	13250.0
62	11372.5
63	9495.0
64	7828.0
65	6161.0
66	5074.5
67	3988.0
68	3090.5
69	2193.0
70	1913.5
71	1634.0
72	1387.0
73	1140.0
74	872.5
75	448.0
76	291.0
77	215.0
78	139.0
79	141.5
80	144.0
81	106.0
82	68.0
83	46.5
84	25.0
85	16.5
86	8.0
87	4.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	637416.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.585082709358026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.72086610799907	35.7404789317472
2	10.560607753579639	9.839335713231904
3	3.7400352530413454	5.226895547965375
4	1.9533957629782404	3.6399641272980343
5	1.2989596783367985	3.0256072025720426
6	0.8927077862407093	2.495211963438681
7	0.7087777197426289	2.311292808773239
8	0.5418305005770732	2.019297494706865
9	0.4584016917145643	1.9219212650369355
>10	3.0337837965613295	24.671880523906466
>50	0.05343344873576935	1.727001769763058
>100	0.031113145866760582	2.6454855530298187
>500	0.0023673045768187063	0.7105252408387558
>1k	0.0033818636811695805	3.1107141641599183
>5k	3.3818636811695806E-4	0.914387693531738
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5804	0.9105513510799854	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	3762	0.5901954139839602	No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	3177	0.4984186151587033	Illumina PCR Primer Index 5 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	3170	0.49732043124113606	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTC	1602	0.25132723370608834	No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1530	0.24003162769682596	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGC	1481	0.2323443402738557	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG	1363	0.213832097092009	No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1284	0.2014383071651794	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	1262	0.19798687199568255	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT	1114	0.17476812630997654	No Hit
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	784	0.12299659876752389	Illumina PCR Primer Index 5 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	674	0.10573942292003967	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	647	0.10150357066656626	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5688341679531107E-4	0.0	0.0	0.5221080110947952	0.0
2	1.5688341679531107E-4	0.0	0.0	1.6326857185888022	0.0
3	1.5688341679531107E-4	0.0	0.0	2.3021072580543946	0.0
4	1.5688341679531107E-4	0.0	0.0	3.122764411310667	0.0
5	1.5688341679531107E-4	0.0	0.0	5.160052461814576	0.0
6	1.5688341679531107E-4	0.0	0.0	6.645738418866173	0.0
7	1.5688341679531107E-4	0.0	0.0	8.026469370081704	0.0
8	1.5688341679531107E-4	0.0	0.0	10.51291464287059	0.0
9	1.5688341679531107E-4	0.0	0.0	11.707424978350089	0.0
10	1.5688341679531107E-4	0.0	0.0	13.91461776924332	0.0
11	1.5688341679531107E-4	0.0	0.0	16.69270931385469	0.0
12	1.5688341679531107E-4	0.0	0.0	18.72419267793717	0.0
13	1.5688341679531107E-4	0.0	0.0	19.491980119733423	0.0
14	1.5688341679531107E-4	0.0	0.0	19.80323681865532	0.0
15	1.5688341679531107E-4	0.0	0.0	20.33146328300513	0.0
16	1.5688341679531107E-4	0.0	0.0	21.389798812706303	0.0
17	1.5688341679531107E-4	0.0	0.0	22.762058059414887	0.0
18	1.5688341679531107E-4	0.0	0.0	24.29167137316917	0.0
19	1.5688341679531107E-4	0.0	0.0	25.193594136325412	0.0
20	1.5688341679531107E-4	0.0	0.0	26.034803017181872	0.0
21	1.5688341679531107E-4	0.0	0.0	27.092040362965474	0.0
22	1.5688341679531107E-4	0.0	0.0	28.151317193167415	0.0
23	1.5688341679531107E-4	0.0	0.0	29.31821604729094	0.0
24	1.5688341679531107E-4	0.0	0.0	30.145776070886203	0.0
25	1.5688341679531107E-4	0.0	0.0	30.938664859369705	0.0
26	1.5688341679531107E-4	0.0	0.0	31.650601804786827	0.0
27	3.1376683359062214E-4	0.0	0.0	32.33932000451824	0.0
28	3.1376683359062214E-4	0.0	0.0	33.05596345243922	0.0
29	3.1376683359062214E-4	0.0	0.0	33.81229840480942	0.0
30	4.706502503859332E-4	0.0	0.0	34.65742937108576	0.0
31	4.706502503859332E-4	0.0	0.0	35.428354481217916	0.0
32	4.706502503859332E-4	0.0	0.0	36.206025578272275	0.0
33	4.706502503859332E-4	0.0	0.0	36.92000200810774	0.0
34	4.706502503859332E-4	0.0	0.0	37.58565834557024	0.0
35	4.706502503859332E-4	0.0	0.0	38.270297576464976	0.0
36	4.706502503859332E-4	0.0	0.0	38.989921809305066	0.0
37	4.706502503859332E-4	0.0	0.0	39.71252682706427	0.0
38	6.275336671812443E-4	0.0	0.0	40.39104760470399	0.0
39	7.844170839765553E-4	0.0	0.0	41.089021926026334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTCGG	40	6.8066583E-9	45.000004	2
CACCGGC	40	6.8066583E-9	45.000004	19
CCCGGTC	20	7.0309185E-4	45.000004	30
GTCGCTC	25	3.8888436E-5	45.0	22
CGGCGCA	50	2.1827873E-11	45.0	22
TATCGAG	25	3.8888436E-5	45.0	1
AGTCGAG	25	3.8888436E-5	45.0	1
TAATCGG	25	3.8888436E-5	45.0	2
CGTTTTT	5140	0.0	43.77432	1
CGAATAT	110	0.0	42.954548	14
CTACGAA	115	0.0	41.086956	11
CGGGTCC	45	1.925946E-8	39.999996	6
ACTACGG	90	0.0	39.999996	2
TTCACGG	40	3.4549885E-7	39.375004	2
ATATGCG	40	3.4549885E-7	39.375004	1
TACGAAT	120	0.0	39.374996	12
GCTACGA	120	0.0	39.374996	10
ATTGGGC	150	0.0	39.0	4
CGTGTAT	75	0.0	39.0	22
AGCTACG	125	0.0	37.8	9
>>END_MODULE