##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130182
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3329646187645	33.0	31.0	34.0	31.0	34.0
2	32.45558525756249	34.0	31.0	34.0	31.0	34.0
3	32.42330736968245	34.0	31.0	34.0	31.0	34.0
4	36.002504186446664	37.0	35.0	37.0	35.0	37.0
5	36.065485243735694	37.0	35.0	37.0	35.0	37.0
6	36.11117512405709	37.0	36.0	37.0	35.0	37.0
7	36.14561921002904	37.0	37.0	37.0	35.0	37.0
8	36.1427309459065	37.0	37.0	37.0	35.0	37.0
9	37.927194235762244	39.0	38.0	39.0	35.0	39.0
10	37.64651027023705	39.0	37.0	39.0	35.0	39.0
11	37.534397996650846	39.0	37.0	39.0	35.0	39.0
12	37.212702216896346	39.0	37.0	39.0	35.0	39.0
13	37.02961238880951	39.0	35.0	39.0	33.0	39.0
14	38.19618687683397	40.0	37.0	41.0	33.0	41.0
15	38.39460140418798	40.0	37.0	41.0	34.0	41.0
16	38.45914949839455	40.0	37.0	41.0	35.0	41.0
17	38.44261111367163	40.0	37.0	41.0	35.0	41.0
18	38.41362093069702	40.0	36.0	41.0	35.0	41.0
19	38.306232812524	40.0	36.0	41.0	34.0	41.0
20	38.197861455500764	40.0	36.0	41.0	34.0	41.0
21	38.09038115868553	40.0	35.0	41.0	34.0	41.0
22	38.064801585472644	40.0	35.0	41.0	34.0	41.0
23	38.0562520164078	40.0	35.0	41.0	34.0	41.0
24	37.947089459372265	40.0	35.0	41.0	34.0	41.0
25	37.881711757385816	40.0	35.0	41.0	34.0	41.0
26	37.917692154061235	40.0	35.0	41.0	34.0	41.0
27	37.85093177244166	40.0	35.0	41.0	34.0	41.0
28	37.796830591018725	40.0	35.0	41.0	34.0	41.0
29	37.772964004240215	40.0	35.0	41.0	34.0	41.0
30	37.68878954079673	40.0	35.0	41.0	34.0	41.0
31	37.60174985789126	40.0	35.0	41.0	34.0	41.0
32	37.442664884546254	40.0	35.0	41.0	33.0	41.0
33	37.307461861086786	40.0	35.0	41.0	33.0	41.0
34	37.23425665606612	40.0	35.0	41.0	33.0	41.0
35	37.124733066015274	40.0	35.0	41.0	33.0	41.0
36	37.057819053325346	39.0	35.0	41.0	33.0	41.0
37	36.96802169270713	39.0	35.0	41.0	33.0	41.0
38	36.874614001935754	39.0	35.0	41.0	32.0	41.0
39	36.79277473076155	39.0	35.0	41.0	32.0	41.0
40	36.662172957859	39.0	35.0	41.0	32.0	41.0
41	36.56383370972946	39.0	35.0	41.0	32.0	41.0
42	36.52474228387949	39.0	35.0	41.0	32.0	41.0
43	36.42004270943756	39.0	35.0	41.0	32.0	41.0
44	36.330890599314806	38.0	35.0	41.0	31.0	41.0
45	36.25182436896038	38.0	35.0	41.0	31.0	41.0
46	36.17076093469143	38.0	35.0	41.0	31.0	41.0
47	36.14067228956384	38.0	35.0	41.0	31.0	41.0
48	36.05597548048117	38.0	35.0	41.0	31.0	41.0
49	36.01978768186078	38.0	35.0	40.0	31.0	41.0
50	35.89134442549661	37.0	35.0	40.0	31.0	41.0
51	34.97520394524589	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	6.0
11	2.0
12	3.0
13	5.0
14	6.0
15	10.0
16	20.0
17	21.0
18	43.0
19	76.0
20	123.0
21	184.0
22	265.0
23	384.0
24	593.0
25	755.0
26	967.0
27	1147.0
28	1219.0
29	1440.0
30	1560.0
31	1991.0
32	2590.0
33	3712.0
34	9066.0
35	15293.0
36	8170.0
37	12113.0
38	21648.0
39	46701.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.14994392465932	21.869382863990413	26.155689726690323	11.824983484659937
2	31.00044553010401	24.99577514556544	26.68648507474152	17.31729424958904
3	31.024258346007898	24.840607764514296	26.38613633221183	17.74899755726598
4	29.066998509778617	26.681876142631083	25.062604661166677	19.188520686423622
5	24.954294756571567	31.47900631423699	24.5702170807024	18.99648184848904
6	25.43669631746324	36.298412991043314	23.40876618887404	14.85612450261941
7	80.28836551904257	7.784486334516292	7.369682444577591	4.557465701863546
8	82.87781720975251	6.591541073266657	6.582323209045797	3.9483185079350447
9	76.9645573120708	8.393633528444793	8.977431595765928	5.664377563718486
10	47.48121860165	23.844310273309674	14.637200227373985	14.037270897666343
11	38.2940805948595	22.761211227358622	20.999062850470878	17.945645327310995
12	33.239618380421255	22.84954909280853	25.301500975557296	18.609331551212918
13	24.77915533637523	28.536203161727432	27.482294019142433	19.20234748275491
14	20.323086140941143	30.39897988969289	28.79507151526325	20.48286245410272
15	19.842989046104684	24.12238251063895	36.32299396229893	19.71163448095743
16	22.208907529458756	22.795010062835107	33.39401760612066	21.60206480158547
17	23.025456668356608	23.051573950315714	27.503034213639367	26.419935167688312
18	24.878247376749474	25.04186446666974	28.422516169670153	21.657371986910633
19	27.118188382418463	26.940744496166907	25.095635341291423	20.84543178012321
20	28.1429076216374	25.6402574856739	25.421333210428475	20.79550168226022
21	26.42531225515048	26.118818269806887	25.786975157855924	21.66889431718671
22	23.858137069640964	26.087323900385613	26.07656972546128	23.977969304512143
23	23.897312992579618	26.318538661258856	26.613510316326373	23.170638029835153
24	23.942634158332183	24.702339801201393	29.43571307861302	21.919312961853404
25	22.685163847536526	25.800801954187214	27.77649751885821	23.737536679418046
26	21.24256809697193	28.403312285876698	26.330829146886664	24.02329047026471
27	22.301086171667357	28.091441213070933	28.659108018005565	20.94836459725615
28	20.184818177628244	27.771888586747785	29.619302207678484	22.42399102794549
29	22.33642131784732	26.35617827349403	29.467207448034287	21.840192960624357
30	23.545497841483463	27.54605091333671	27.148914596487995	21.75953664869183
31	26.04584351139174	27.92167888033676	23.551643084297368	22.480834523974128
32	27.251847413620933	27.619025671751857	23.90115376933831	21.227973145288903
33	25.78543884715245	27.863299073604647	24.244519211565347	22.10674286767756
34	22.261910248728704	28.900308798451395	26.326220214776235	22.511560738043663
35	23.549338618242153	26.848565853958306	26.843188766496134	22.758906761303404
36	25.205481556590005	28.56462490974175	25.051850486242337	21.178043047425913
37	25.219308352921292	29.252123949547553	26.064279239833464	19.464288457697684
38	23.991027945491698	29.20065754098109	23.427201917315756	23.38111259621146
39	24.697730869090964	27.90708392865373	25.01421087400716	22.380974328248147
40	25.243121168825184	25.93215651933447	24.988093592048056	23.83662871979229
41	20.99368576300871	25.82922370220153	26.152617105283372	27.024473429506386
42	23.474059393771796	26.03816195787436	25.6702155443917	24.817563103962144
43	24.85597087154906	24.18767571553671	27.08515770229371	23.871195710620515
44	22.571476855479254	27.823354995314254	26.197170115684198	23.4079980335223
45	22.408627920910725	28.334946459571984	25.033414757800614	24.223010861716674
46	23.55548386105606	28.252753836935984	25.265397674025596	22.926364627982363
47	22.685932002888265	26.274753806209766	28.112181407567867	22.927132783334102
48	24.160022122874132	23.942634158332183	28.49856354949225	23.39878016930144
49	23.927271051297414	24.159253967522393	28.749750349510684	23.163724631669506
50	21.86093315512129	26.46295186738566	28.538507627782643	23.137607349710407
51	21.65429936550368	26.87237866986219	25.833832634311964	25.639489330322164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	146.5
2	241.0
3	467.0
4	693.0
5	509.5
6	326.0
7	341.5
8	357.0
9	384.0
10	411.0
11	440.0
12	469.0
13	439.0
14	409.0
15	384.5
16	360.0
17	381.0
18	402.0
19	438.5
20	475.0
21	495.5
22	516.0
23	474.5
24	433.0
25	478.5
26	817.5
27	1111.0
28	1260.5
29	1410.0
30	1642.5
31	1875.0
32	2128.5
33	2382.0
34	2501.5
35	2621.0
36	3161.5
37	3702.0
38	3710.0
39	3718.0
40	4800.0
41	5882.0
42	6956.5
43	8031.0
44	8690.5
45	9350.0
46	9948.5
47	10547.0
48	10583.5
49	10620.0
50	10130.0
51	9640.0
52	9103.0
53	8566.0
54	7680.0
55	6794.0
56	7048.5
57	7303.0
58	6939.5
59	6576.0
60	6335.0
61	6094.0
62	5548.0
63	5002.0
64	4637.0
65	4272.0
66	3570.5
67	2869.0
68	2534.0
69	2199.0
70	1831.5
71	1464.0
72	1231.0
73	998.0
74	852.5
75	511.5
76	316.0
77	303.5
78	291.0
79	177.0
80	63.0
81	55.0
82	47.0
83	51.0
84	55.0
85	30.5
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	130182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.618948856216676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50141296156744	23.649198813968138
2	6.996514694800301	4.56437910002919
3	3.1108703843255463	3.0441996589390237
4	2.0346646571213265	2.654744895607688
5	1.7873963828183872	2.9151495598469834
6	1.3587980406932931	2.659353827718118
7	1.2127920120572722	2.7692000430166996
8	1.1397889977392615	2.9742975219308354
9	0.9466842501883949	2.7791860625892983
>10	8.85926902788244	50.132890875850734
>50	0.03296910324039186	0.8073312746769907
>100	0.018839487565938208	1.0500683658263046
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	360	0.2765359266258008	No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	193	0.14825398288549876	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	177	0.1359634972576854	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	176	0.13519534190594706	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	135	0.10370097248467529	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06606136024949685	0.0
2	0.0	0.0	0.0	0.40942680247653285	0.0
3	0.0	0.0	0.0	0.5799572905624434	0.0
4	0.0	0.0	0.0	0.7620101089244289	0.0
5	0.0	0.0	0.0	1.3281406031555822	0.0
6	0.0	0.0	0.0	1.7406400270390683	0.0
7	0.0	0.0	0.0	2.161589159791676	0.0
8	0.0	0.0	0.0	2.9950377164277704	0.0
9	0.0	0.0	0.0	3.445176752546435	0.0
10	0.0	0.0	0.0	4.244826473706042	0.0
11	0.0	0.0	0.0	5.0191270682582845	0.0
12	0.0	0.0	0.0	5.717380282988431	0.0
13	0.0	0.0	0.0	5.968567083006867	0.0
14	0.0	0.0	0.0	6.0553686377533	0.0
15	0.0	0.0	0.0	6.204390775990536	0.0
16	0.0	0.0	0.0	6.538538353996712	0.0
17	0.0	0.0	0.0	6.972546127728872	0.0
18	0.0	0.0	0.0	7.5563441950500065	0.0
19	0.0	0.0	0.0	7.820589636047995	0.0
20	0.0	0.0	0.0	8.074849057473383	0.0
21	0.0	0.0	0.0	8.365979935782212	0.0
22	0.0	0.0	0.0	8.675546542532762	0.0
23	0.0	0.0	0.0	8.95822771197247	0.0
24	0.0	0.0	0.0	9.21171897804612	0.0
25	0.0	0.0	0.0	9.422193544422424	0.0
26	0.0	0.0	0.0	9.624218401929607	0.0
27	0.0	0.0	0.0	9.828547725492003	0.0
28	0.0	0.0	0.0	10.045167534682214	0.0
29	0.0	0.0	0.0	10.274077829500238	0.0
30	0.0	0.0	0.0	10.518351231353028	0.0
31	0.0	0.0	0.0	10.720376088860212	0.0
32	0.0	0.0	0.0	10.952359005085189	0.0
33	0.0	0.0	0.0	11.197400562289717	0.0
34	0.0	0.0	0.0	11.430151633866434	0.0
35	0.0	0.0	0.0	11.65752561798098	0.0
36	0.0	0.0	0.0	11.871840961115977	0.0
37	0.0	0.0	0.0	12.108432809451383	0.0
38	0.0	0.0	0.0	12.355778832711128	0.0
39	0.0	0.0	0.0	12.634619225392143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGG	20	7.0102257E-4	45.000004	2
CGGCAAA	20	7.0102257E-4	45.000004	28
TACGGGT	20	7.0102257E-4	45.000004	4
ATACTTC	20	7.0102257E-4	45.000004	42
ATCCCCG	20	7.0102257E-4	45.000004	12
CTTGACT	20	7.0102257E-4	45.000004	16
AGCGATT	20	7.0102257E-4	45.000004	22
CATACTT	20	7.0102257E-4	45.000004	41
ATTGGGC	20	7.0102257E-4	45.000004	4
CGTAAGG	20	7.0102257E-4	45.000004	2
TACTAGG	20	7.0102257E-4	45.000004	2
TCATACT	20	7.0102257E-4	45.000004	40
TCGAGCC	20	7.0102257E-4	45.000004	12
TCCCCGA	20	7.0102257E-4	45.000004	13
GGCGATA	40	6.7357178E-9	45.000004	8
ATACGGG	20	7.0102257E-4	45.000004	3
CGCAAGG	20	7.0102257E-4	45.000004	2
CGCAAAA	20	7.0102257E-4	45.000004	45
CGTCATA	20	7.0102257E-4	45.000004	38
TGCGTTG	20	7.0102257E-4	45.000004	1
>>END_MODULE