SRR3550185_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398605
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1172	0.29402541363003476	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	796	0.19969644133917036	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	614	0.15403720475157112	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG	510	0.1279462124158001	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC	480	0.1204199646266354	No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	458	0.1149007162479146	Illumina Paired End PCR Primer 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	40	6.7957444E-9	45.0	24
TCGCACG	20	7.027736E-4	45.0	1
GATCGAG	20	7.027736E-4	45.0	1
GCGAATA	20	7.027736E-4	45.0	1
ATTACGG	20	7.027736E-4	45.0	2
TCGGACG	25	3.8862054E-5	45.0	1
GCGATAT	25	3.8862054E-5	45.0	9
GCGTAAG	20	7.027736E-4	45.0	1
GCCCGAT	20	7.027736E-4	45.0	39
TCGTTAG	30	2.1619217E-6	44.999996	1
ACCGGGT	30	2.1619217E-6	44.999996	4
CGAAAGG	30	2.1619217E-6	44.999996	2
CTAACGG	30	2.1619217E-6	44.999996	2
TCGTACG	80	0.0	42.1875	30
TCGTCCC	50	1.0786607E-9	40.5	38
CGTTGAT	45	1.9228537E-8	40.0	25
TACGGGT	40	3.450641E-7	39.375	4
AGGGCCG	40	3.450641E-7	39.375	6
CGGGACC	105	0.0	38.57143	6
CGTATGG	35	6.238026E-6	38.571426	2