##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398605
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17473187742251	33.0	31.0	34.0	30.0	34.0
2	32.3095846765595	34.0	31.0	34.0	30.0	34.0
3	32.29832290111765	34.0	31.0	34.0	30.0	34.0
4	35.877665859685656	37.0	35.0	37.0	35.0	37.0
5	35.96604658747381	37.0	35.0	37.0	35.0	37.0
6	36.002428469286635	37.0	35.0	37.0	35.0	37.0
7	36.06889025476349	37.0	35.0	37.0	35.0	37.0
8	36.048514193248955	37.0	36.0	37.0	35.0	37.0
9	37.82231532469488	39.0	38.0	39.0	35.0	39.0
10	37.51857101641976	39.0	37.0	39.0	35.0	39.0
11	37.45215438842965	39.0	37.0	39.0	35.0	39.0
12	37.29991093940116	39.0	37.0	39.0	35.0	39.0
13	37.23105078962883	39.0	37.0	39.0	34.0	39.0
14	38.43343410142873	40.0	38.0	41.0	34.0	41.0
15	38.57772481529334	40.0	38.0	41.0	35.0	41.0
16	38.640059708232464	40.0	38.0	41.0	35.0	41.0
17	38.60915693481015	40.0	38.0	41.0	35.0	41.0
18	38.56060260157299	40.0	38.0	41.0	35.0	41.0
19	38.50109757780259	40.0	37.0	41.0	35.0	41.0
20	38.408211136337975	40.0	37.0	41.0	34.0	41.0
21	38.31535229111527	40.0	37.0	41.0	34.0	41.0
22	38.304429196823925	40.0	37.0	41.0	34.0	41.0
23	38.259394638802824	40.0	37.0	41.0	34.0	41.0
24	38.18900164323077	40.0	37.0	41.0	34.0	41.0
25	38.09993100939526	40.0	36.0	41.0	34.0	41.0
26	38.116077319652284	40.0	36.0	41.0	34.0	41.0
27	38.06763588013196	40.0	36.0	41.0	34.0	41.0
28	38.00551172213093	40.0	36.0	41.0	34.0	41.0
29	37.9640847455501	40.0	36.0	41.0	34.0	41.0
30	37.87033529433901	40.0	36.0	41.0	34.0	41.0
31	37.83784197388392	40.0	36.0	41.0	34.0	41.0
32	37.71344564167534	40.0	36.0	41.0	33.0	41.0
33	37.633210822744324	40.0	36.0	41.0	33.0	41.0
34	37.566327567391276	40.0	35.0	41.0	33.0	41.0
35	37.45197375848271	40.0	35.0	41.0	33.0	41.0
36	37.37739867788914	40.0	35.0	41.0	33.0	41.0
37	37.317281519298554	40.0	35.0	41.0	33.0	41.0
38	37.21207461020308	40.0	35.0	41.0	33.0	41.0
39	37.14279549930382	39.0	35.0	41.0	33.0	41.0
40	37.03675066795449	39.0	35.0	41.0	32.0	41.0
41	36.92795624741285	39.0	35.0	41.0	32.0	41.0
42	36.89490849337063	39.0	35.0	41.0	32.0	41.0
43	36.80512788349368	39.0	35.0	41.0	32.0	41.0
44	36.742200925728476	39.0	35.0	41.0	32.0	41.0
45	36.66093501085034	39.0	35.0	41.0	32.0	41.0
46	36.557188193825965	39.0	35.0	41.0	31.0	41.0
47	36.4928463014764	39.0	35.0	41.0	31.0	41.0
48	36.42872768781124	38.0	35.0	41.0	31.0	41.0
49	36.36898935035938	38.0	35.0	41.0	31.0	41.0
50	36.25559137492004	38.0	35.0	40.0	31.0	41.0
51	35.322399367795185	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	12.0
10	8.0
11	9.0
12	4.0
13	11.0
14	15.0
15	11.0
16	38.0
17	42.0
18	78.0
19	163.0
20	259.0
21	479.0
22	664.0
23	1032.0
24	1542.0
25	2025.0
26	2558.0
27	2967.0
28	3366.0
29	4090.0
30	4938.0
31	6305.0
32	8167.0
33	11282.0
34	23376.0
35	36102.0
36	28160.0
37	41662.0
38	72151.0
39	146905.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.55768241743079	21.583020784987646	25.12938874324206	11.729908054339509
2	30.630323252342546	25.510718631226403	25.709913322712964	18.14904479371809
3	32.09217144792464	24.596530399769197	26.173279311599202	17.138018840706966
4	28.79818366553355	26.474830973018403	25.700630950439656	19.02635441100839
5	25.084482131433372	30.46600017561245	24.714441615132777	19.735076077821404
6	25.550356869582668	36.291315964425934	22.87954240413442	15.278784761856976
7	79.19995986001179	7.03177330941659	8.522221246597509	5.24604558397411
8	79.8861027834573	6.502678089838311	7.794683960311587	5.8165351663927956
9	73.73163909133102	8.333814176942084	10.381706200373804	7.552840531353095
10	42.25034808896025	24.29849098731827	17.21403394337753	16.237126980343948
11	32.24570690282359	25.026530023456807	22.869256532155894	19.858506541563703
12	29.30545276652325	21.489695312402002	28.32478267959509	20.88006924147966
13	24.460054439859007	24.725730986816522	29.894256218562237	20.919958354762233
14	19.951832014149346	27.79016821163809	29.027734223103074	23.230265551109493
15	19.578028373954165	24.022528568382235	34.62475382897856	21.77468922868504
16	21.64072201803791	23.58550444675807	31.287866434189237	23.48590710101479
17	21.221510016181433	24.683834874123505	28.159205228233464	25.935449881461597
18	22.70593695513102	24.85593507356907	29.41056936064525	23.02755861065466
19	24.366227217420754	26.3376023883293	25.76786543068953	23.528304963560416
20	25.66776633509364	26.026266604784187	26.391289622558673	21.914677437563505
21	24.658496506566653	27.546819533121763	25.991896739880332	21.802787220431256
22	23.09680009031497	25.281920698435794	25.832591161676344	25.788688049572883
23	22.739052445403342	26.526009457984724	26.295957150562586	24.438980946049345
24	22.946526009457983	24.634161638715018	28.302705686080206	24.116606665746794
25	21.97037167120332	26.556867073920298	26.75556001555425	24.717201239322137
26	21.59155053248203	27.245016996776254	26.579696692214092	24.583735778527615
27	22.86122853451412	26.218185923407884	26.28667477828928	24.633910763788712
28	20.95909484326589	26.87648173003349	27.226201377303344	24.938222049397275
29	21.689893503593783	25.51197300585793	26.843115364834862	25.955018125713426
30	23.475119479183654	25.6386648436422	25.51699050438404	25.369225172790106
31	24.259354498814616	25.5608936164875	23.085510718631227	27.094241166066656
32	25.0719383851181	25.81176854279299	23.39082550394501	25.725467568143902
33	23.478129978299318	25.565660240087308	24.947504421670576	26.0087053599428
34	21.806048594473225	24.94298867299708	26.593996563013512	26.65696616951619
35	22.294752950916322	25.3142835639292	26.337351513402997	26.053611971751483
36	23.242809297424767	26.160735565283925	26.715670902271672	23.880784235019632
37	23.402616625481365	27.575670149646893	26.732981272186752	22.28873195268499
38	22.051906022252606	27.09198329172991	27.51922329122816	23.336887394789326
39	23.545615333475496	25.26787170256269	28.52874399468145	22.657768969280365
40	24.867475320179125	23.482645726972816	29.272337276250926	22.377541676597133
41	21.233301137717792	24.221723259868792	28.264823572208076	26.28015203020534
42	21.77243135434829	24.660503505977093	27.701358487725948	25.86570665194867
43	22.516526385770376	24.172551774312918	28.530249244239286	24.780672595677427
44	22.935487512700544	24.9896514092899	26.7078937795562	25.366967298453357
45	21.986427666486875	25.092259254148846	26.918628717652815	26.00268436171147
46	22.965592503857202	25.54358324657242	26.952496832704053	24.53832741686632
47	22.479396896677162	24.06342118137003	29.041030594197263	24.416151327755546
48	21.89811969242734	23.092535216567782	28.815494035448623	26.19385105555625
49	22.673072339785	22.824099045420905	29.55708031760766	24.945748297186437
50	21.326124860450825	23.804267382496455	29.07665483373264	25.79295292332008
51	21.438516827435684	23.543357459138747	26.955758206746026	28.062367506679546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	113.0
1	314.5
2	516.0
3	934.5
4	1353.0
5	1062.0
6	771.0
7	733.5
8	696.0
9	734.5
10	773.0
11	784.0
12	795.0
13	816.5
14	838.0
15	878.0
16	918.0
17	853.5
18	789.0
19	801.0
20	813.0
21	954.0
22	1095.0
23	1184.5
24	1274.0
25	1559.5
26	2466.0
27	3087.0
28	3733.5
29	4380.0
30	5395.0
31	6410.0
32	6972.0
33	7534.0
34	8486.5
35	9439.0
36	10126.5
37	10814.0
38	11328.5
39	11843.0
40	13419.0
41	14995.0
42	17939.0
43	20883.0
44	23251.0
45	25619.0
46	30058.5
47	34498.0
48	35464.5
49	36431.0
50	35833.0
51	35235.0
52	30974.5
53	26714.0
54	24427.0
55	22140.0
56	21412.5
57	20685.0
58	20507.0
59	20329.0
60	19204.0
61	18079.0
62	16852.0
63	15625.0
64	13917.5
65	12210.0
66	11010.0
67	9810.0
68	8365.5
69	6921.0
70	5892.5
71	4864.0
72	3889.0
73	2914.0
74	2564.0
75	1692.5
76	1171.0
77	863.0
78	555.0
79	402.5
80	250.0
81	254.5
82	259.0
83	163.5
84	68.0
85	37.5
86	7.0
87	10.5
88	14.0
89	16.0
90	18.0
91	9.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.10644917416265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50515168332656	22.553778157023512
2	8.637163543491104	5.373429775490732
3	3.3156742108445547	3.0941655395315397
4	1.865419800968453	2.3210634490920716
5	1.3684922196596803	2.1284466838040434
6	1.1661420636346413	2.1764723299382465
7	0.9420898050993389	2.0513548047873567
8	0.8030761545144041	1.998467806670744
9	0.6980364355176273	1.9542091412828464
>10	8.571912417232694	51.74960804198791
>50	0.09290950036151001	1.8798115815587249
>100	0.03070053055423809	1.9421368853323295
>500	0.0024237260963872174	0.482518480827908
>1k	8.079086987957391E-4	0.2945373226720355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1172	0.29402541363003476	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	796	0.19969644133917036	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	614	0.15403720475157112	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG	510	0.1279462124158001	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC	480	0.1204199646266354	No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	458	0.1149007162479146	Illumina Paired End PCR Primer 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11866384014249695	0.0
2	0.0	0.0	0.0	0.8148417606402328	0.0
3	0.0	0.0	0.0	1.0903024297236612	0.0
4	0.0	0.0	0.0	1.4430325761091807	0.0
5	0.0	0.0	0.0	2.5784924925678303	0.0
6	0.0	0.0	0.0	3.451537236110937	0.0
7	0.0	0.0	0.0	4.348415097653065	0.0
8	0.0	0.0	0.0	6.064650468508925	0.0
9	0.0	0.0	0.0	6.88325535304374	0.0
10	0.0	0.0	0.0	8.362413918540911	0.0
11	0.0	0.0	0.0	9.699075525896564	0.0
12	0.0	0.0	0.0	10.79364282936742	0.0
13	0.0	0.0	0.0	11.184505964551374	0.0
14	0.0	0.0	0.0	11.331518671366391	0.0
15	0.0	0.0	0.0	11.603467091481543	0.0
16	0.0	0.0	0.0	12.125788688049573	0.0
17	0.0	0.0	0.0	12.742690131834774	0.0
18	0.0	0.0	0.0	13.544235521380816	0.0
19	0.0	0.0	0.0	13.919042661281217	0.0
20	0.0	0.0	0.0	14.281556929792652	0.0
21	0.0	0.0	0.0	14.72384942486923	0.0
22	2.5087492630549037E-4	0.0	0.0	15.18897153823961	0.0
23	5.017498526109807E-4	0.0	0.0	15.678428519461622	0.0
24	5.017498526109807E-4	0.0	0.0	16.08459502515021	0.0
25	5.017498526109807E-4	0.0	0.0	16.439081296019868	0.0
26	5.017498526109807E-4	0.0	0.0	16.74565045596518	0.0
27	5.017498526109807E-4	0.0	0.0	17.079063233025174	0.0
28	5.017498526109807E-4	0.0	0.0	17.387890267307235	0.0
29	5.017498526109807E-4	0.0	0.0	17.72406266855659	0.0
30	5.017498526109807E-4	0.0	0.0	18.107901305803992	0.0
31	0.0010034997052219615	0.0	0.0	18.456868328294927	0.0
32	0.0015052495578329423	0.0	0.0	18.79228810476537	0.0
33	0.0015052495578329423	0.0	0.0	19.095345015742403	0.0
34	0.0015052495578329423	0.0	0.0	19.42072979516062	0.0
35	0.0015052495578329423	0.0	0.0	19.77070031735678	0.0
36	0.0015052495578329423	0.0	0.0	20.091067598248895	0.0
37	0.0015052495578329423	0.0	0.0	20.403156006572924	0.0
38	0.0015052495578329423	0.0	0.0	20.707467292181484	0.0
39	0.0015052495578329423	0.0	0.0	21.021311824989652	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	40	6.7957444E-9	45.0	24
TCGCACG	20	7.027736E-4	45.0	1
GATCGAG	20	7.027736E-4	45.0	1
GCGAATA	20	7.027736E-4	45.0	1
ATTACGG	20	7.027736E-4	45.0	2
TCGGACG	25	3.8862054E-5	45.0	1
GCGATAT	25	3.8862054E-5	45.0	9
GCGTAAG	20	7.027736E-4	45.0	1
GCCCGAT	20	7.027736E-4	45.0	39
TCGTTAG	30	2.1619217E-6	44.999996	1
ACCGGGT	30	2.1619217E-6	44.999996	4
CGAAAGG	30	2.1619217E-6	44.999996	2
CTAACGG	30	2.1619217E-6	44.999996	2
TCGTACG	80	0.0	42.1875	30
TCGTCCC	50	1.0786607E-9	40.5	38
CGTTGAT	45	1.9228537E-8	40.0	25
TACGGGT	40	3.450641E-7	39.375	4
AGGGCCG	40	3.450641E-7	39.375	6
CGGGACC	105	0.0	38.57143	6
CGTATGG	35	6.238026E-6	38.571426	2
>>END_MODULE