FastQCFastQC Report
Sat 18 Jun 2016
SRR3550183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238698
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC6200.2597424360488986No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.2077939488391189No Hit
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC4390.1839144022991395No Hit
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT3520.14746667336969727Illumina Single End Adapter 1 (95% over 21bp)
GCTCTTGGGCTGTCTCTTATACACATCTGACGCCGGTTTGTCTCGTATGCC3330.13950682452303748No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3280.137412127458127No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT2800.11730303563498645No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2720.11395152033112971No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2690.11269470209218342No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC2430.10180227735464896No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG207.0220424E-445.0000041
AACCCCG207.0220424E-445.00000411
TAGAATA207.0220424E-445.00000411
ACGGGTC207.0220424E-445.0000045
GTATGCG207.0220424E-445.0000041
AGCGCAA207.0220424E-445.00000426
GCGATTA302.158251E-645.00000423
ACATCGG207.0220424E-445.0000042
GGCGATA600.045.0000048
AGGGCGC302.158251E-645.0000046
GCGTAAG207.0220424E-445.0000041
CTCGTAT502.1827873E-1145.042
ATAGGGT351.2065175E-745.04
TCGTGTA253.88149E-545.017
ATTCAAT253.88149E-545.014
CAGATGA253.88149E-545.034
TGATTGG253.88149E-545.02
GTATACG253.88149E-545.01
TAGGGAC1600.043.5937545
TAGATGG556.002665E-1140.9090922