##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	238698
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24154789734309	33.0	31.0	34.0	30.0	34.0
2	32.367024440925356	34.0	31.0	34.0	30.0	34.0
3	32.353894879722496	34.0	31.0	34.0	30.0	34.0
4	35.92845352705092	37.0	35.0	37.0	35.0	37.0
5	36.02015098576444	37.0	35.0	37.0	35.0	37.0
6	36.047474214279134	37.0	35.0	37.0	35.0	37.0
7	36.11733236139389	37.0	36.0	37.0	35.0	37.0
8	36.119887891813086	37.0	37.0	37.0	35.0	37.0
9	37.88060645669423	39.0	38.0	39.0	35.0	39.0
10	37.608010959455044	39.0	37.0	39.0	35.0	39.0
11	37.52010909182314	39.0	37.0	39.0	35.0	39.0
12	37.30790371096532	39.0	37.0	39.0	35.0	39.0
13	37.21677182045933	39.0	37.0	39.0	34.0	39.0
14	38.40797157915022	40.0	38.0	41.0	34.0	41.0
15	38.55449982823484	40.0	38.0	41.0	35.0	41.0
16	38.61226319449681	40.0	38.0	41.0	35.0	41.0
17	38.6040436032141	40.0	38.0	41.0	35.0	41.0
18	38.56297078316534	40.0	37.0	41.0	35.0	41.0
19	38.48173005219985	40.0	37.0	41.0	35.0	41.0
20	38.39808879839798	40.0	37.0	41.0	34.0	41.0
21	38.28732121760551	40.0	36.0	41.0	34.0	41.0
22	38.275921876178266	40.0	36.0	41.0	34.0	41.0
23	38.27412043670245	40.0	36.0	41.0	34.0	41.0
24	38.204086335034226	40.0	36.0	41.0	34.0	41.0
25	38.1087776185808	40.0	36.0	41.0	34.0	41.0
26	38.14909215829207	40.0	36.0	41.0	34.0	41.0
27	38.09826642870908	40.0	36.0	41.0	34.0	41.0
28	38.04861791887657	40.0	36.0	41.0	34.0	41.0
29	37.97511499886886	40.0	36.0	41.0	34.0	41.0
30	37.87328339575531	40.0	36.0	41.0	34.0	41.0
31	37.84531918993875	40.0	36.0	41.0	34.0	41.0
32	37.644056506548026	40.0	35.0	41.0	33.0	41.0
33	37.54667404000033	40.0	35.0	41.0	33.0	41.0
34	37.49089225716177	40.0	35.0	41.0	33.0	41.0
35	37.36229880434691	40.0	35.0	41.0	33.0	41.0
36	37.25011520833857	40.0	35.0	41.0	33.0	41.0
37	37.15932684815122	39.0	35.0	41.0	33.0	41.0
38	37.06647311665787	39.0	35.0	41.0	33.0	41.0
39	37.004775909307995	39.0	35.0	41.0	32.0	41.0
40	36.933179163629354	39.0	35.0	41.0	32.0	41.0
41	36.85813035718775	39.0	35.0	41.0	32.0	41.0
42	36.79547377858214	39.0	35.0	41.0	32.0	41.0
43	36.66985898499359	39.0	35.0	41.0	32.0	41.0
44	36.56983301074999	39.0	35.0	41.0	31.0	41.0
45	36.45508969492832	39.0	35.0	41.0	31.0	41.0
46	36.41004532924448	39.0	35.0	41.0	31.0	41.0
47	36.33693202289085	38.0	35.0	41.0	31.0	41.0
48	36.26730848184736	38.0	35.0	41.0	31.0	41.0
49	36.180575455177674	38.0	35.0	41.0	31.0	41.0
50	36.069351230425056	38.0	35.0	40.0	31.0	41.0
51	35.12773462701824	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	4.0
10	8.0
11	5.0
12	6.0
13	5.0
14	15.0
15	10.0
16	19.0
17	34.0
18	52.0
19	95.0
20	159.0
21	251.0
22	410.0
23	582.0
24	985.0
25	1346.0
26	1545.0
27	1901.0
28	2126.0
29	2359.0
30	2982.0
31	3622.0
32	4667.0
33	6909.0
34	14730.0
35	23432.0
36	16078.0
37	24282.0
38	42974.0
39	86991.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7196625024089	21.708602501906174	24.944909467192854	11.62682552849207
2	30.955852164659948	24.85567537222767	26.10956103528308	18.078911427829308
3	31.97261811996749	24.34792080369337	26.817568643222817	16.861892433116324
4	28.699025546925405	26.473619385164515	25.502517825872022	19.324837242038058
5	24.812943552103494	31.510109008035258	24.779428399064926	18.897519040796322
6	25.860292084558733	35.730923593829864	23.108698020092334	15.300086301519075
7	80.40159532128463	7.672875348767061	7.394699578546951	4.5308297514013525
8	81.80713705183956	6.181869977963786	6.8764715246881	5.13452144550855
9	74.81545718858139	9.041131471566583	9.75290953422316	6.390501805628871
10	43.88893078283019	24.54272763073004	15.746256776345005	15.822084810094763
11	33.87963032786199	23.86949199406782	22.362985864984207	19.887891813085993
12	30.30691501395068	22.127122975475285	27.970070968336557	19.595891042237472
13	25.157311749574777	26.651668635681908	28.195460372520927	19.99555924222239
14	19.9859236357238	29.96003318000151	27.76437171656235	22.28967146771234
15	19.59547210282449	24.472764748762035	35.20012735758154	20.73163579083193
16	21.474834309462164	23.470242733495883	31.695280228573342	23.359642728468607
17	21.689331288908996	23.75009426136792	27.93027172410326	26.63030272561982
18	23.11037377774426	24.706532941206046	29.75014453409748	22.432948746952217
19	25.517180705326396	26.53813605476376	26.111236792935006	21.83344644697484
20	26.637424695640515	25.871184509296263	26.535203478872887	20.956187316190334
21	24.40238292738104	27.783223990146546	25.63280798330945	22.18158509916296
22	22.670068454700083	25.887104206989587	24.907623859437447	26.535203478872887
23	23.270827572916406	26.791594399617928	25.758908746617067	24.178669280848602
24	23.434213943979422	24.40405868503297	28.779880853630946	23.38184651735666
25	21.84559568995132	26.553636813044097	27.196289872558634	24.404477624445953
26	21.306839604856346	27.785318687211458	26.263311799847504	24.644529908084692
27	22.946568467268264	27.12967850589448	26.8297178861993	23.09403514063796
28	21.43712976229378	26.76436333777409	28.25830128446824	23.54020561546389
29	22.332403287836513	25.649984499241718	27.555320949484287	24.462291263437482
30	23.336601060754596	26.797040611986695	26.372236047222852	23.49412228003586
31	26.93612849709675	26.1183587629557	23.729985169544783	23.21552757040277
32	26.814636067331943	25.58169737492564	24.486589749390443	23.117076808351978
33	25.141810991294438	26.810446673202122	24.88416325231045	23.16357908319299
34	23.19583741799261	25.462299642225737	27.193776236080737	24.148086703700912
35	24.98428977201317	24.312729893002874	26.743835306537967	23.959145028445985
36	26.49456635581362	26.195024675531425	26.72414515412781	20.586263814527143
37	24.894636737635004	27.399056548441962	27.89382399517382	19.812482718749212
38	24.26790337581379	26.693562576980117	27.341242909450436	21.69729113775566
39	23.538529857811962	24.94993674014864	29.37435588065254	22.137177521386857
40	24.957896588995297	24.084407912927634	29.165305113574476	21.792390384502593
41	22.503330568333208	24.47946777936975	27.435504277371408	25.58169737492564
42	24.00941775800384	24.688518546447813	26.606842118492825	24.695221577055527
43	24.849391281032936	23.052979078165716	28.43970205028949	23.65792759051186
44	23.732917745435657	24.609757936807178	27.35423003125288	24.303094286504287
45	21.9302214513737	26.231472404460867	27.040444410929293	24.79786173323614
46	23.913061693017955	25.89757769231414	27.562861858917962	22.626498755749942
47	23.520934402466715	24.229360949819437	29.533133918172755	22.716570729541093
48	23.792826081492095	22.88624119179884	29.0429748049837	24.27795792172536
49	23.612263194496812	23.552773797853355	29.576284677709907	23.258678329939926
50	22.174463129142264	24.687680667621848	28.829315704362834	24.30854049887305
51	22.40781238217329	24.24821322340363	27.18204593251724	26.161928461905838
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	145.0
1	261.5
2	378.0
3	695.0
4	1012.0
5	761.0
6	510.0
7	511.5
8	513.0
9	535.5
10	558.0
11	615.0
12	672.0
13	642.5
14	613.0
15	603.5
16	594.0
17	566.0
18	538.0
19	564.5
20	591.0
21	681.0
22	771.0
23	794.0
24	817.0
25	1082.5
26	1650.5
27	1953.0
28	2313.0
29	2673.0
30	3125.0
31	3577.0
32	4031.0
33	4485.0
34	5004.0
35	5523.0
36	6074.5
37	6626.0
38	6753.5
39	6881.0
40	8207.5
41	9534.0
42	11327.5
43	13121.0
44	14680.0
45	16239.0
46	17959.0
47	19679.0
48	20658.0
49	21637.0
50	21076.5
51	20516.0
52	18116.0
53	15716.0
54	14108.5
55	12501.0
56	11970.5
57	11440.0
58	11631.5
59	11823.0
60	11316.5
61	10810.0
62	10163.5
63	9517.0
64	8408.0
65	7299.0
66	6416.5
67	5534.0
68	4786.0
69	4038.0
70	3541.5
71	3045.0
72	2636.5
73	2228.0
74	1836.5
75	1169.5
76	894.0
77	672.5
78	451.0
79	350.5
80	250.0
81	186.5
82	123.0
83	87.0
84	51.0
85	38.5
86	26.0
87	14.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	238698.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.80002346060712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.41643569667787	23.752607897845813
2	8.179530737102935	5.365776001474667
3	3.173974684837725	3.1231933237815146
4	1.9388706525487593	2.543800115627278
5	1.4880002043605431	2.440322080620701
6	1.2721443807236916	2.503581931980997
7	1.0946061589158673	2.513217538479585
8	0.9617718059085743	2.5236910238041377
9	0.8851366022505205	2.6129251187693234
>10	8.51800288659267	49.335143151597414
>50	0.04342661540623044	0.9673311045756562
>100	0.026822321280318806	2.0586682753940124
>500	0.0012772533943008952	0.2597424360488986
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	620	0.2597424360488986	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	496	0.2077939488391189	No Hit
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	439	0.1839144022991395	No Hit
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT	352	0.14746667336969727	Illumina Single End Adapter 1 (95% over 21bp)
GCTCTTGGGCTGTCTCTTATACACATCTGACGCCGGTTTGTCTCGTATGCC	333	0.13950682452303748	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	328	0.137412127458127	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	280	0.11730303563498645	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	272	0.11395152033112971	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	269	0.11269470209218342	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	243	0.10180227735464896	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1893941298209455E-4	0.0	0.0	0.15752121928126755	0.0
2	4.1893941298209455E-4	0.0	0.0	0.8026879152736931	0.0
3	4.1893941298209455E-4	0.0	0.0	1.032685653000863	0.0
4	4.1893941298209455E-4	0.0	0.0	1.3091856655690455	0.0
5	4.1893941298209455E-4	0.0	0.0	2.1872826751795156	0.0
6	4.1893941298209455E-4	0.0	0.0	2.790974369286714	0.0
7	4.1893941298209455E-4	0.0	0.0	3.4009501545886436	0.0
8	4.1893941298209455E-4	0.0	0.0	4.66237672707773	0.0
9	4.1893941298209455E-4	0.0	0.0	5.320949484285583	0.0
10	4.1893941298209455E-4	0.0	0.0	6.701773789474566	0.0
11	4.1893941298209455E-4	0.0	0.0	7.939739754836656	0.0
12	4.1893941298209455E-4	0.0	0.0	8.95482995249227	0.0
13	4.1893941298209455E-4	0.0	0.0	9.322658757090549	0.0
14	4.1893941298209455E-4	0.0	0.0	9.483950431088656	0.0
15	4.1893941298209455E-4	0.0	0.0	9.731543624161073	0.0
16	4.1893941298209455E-4	0.0	0.0	10.234270919739588	0.0
17	4.1893941298209455E-4	0.0	0.0	10.85681488743098	0.0
18	4.1893941298209455E-4	0.0	0.0	11.651124014445031	0.0
19	4.1893941298209455E-4	0.0	0.0	11.989208120721582	0.0
20	4.1893941298209455E-4	0.0	0.0	12.331481621127953	0.0
21	8.378788259641891E-4	0.0	0.0	12.739947548785494	0.0
22	8.378788259641891E-4	0.0	0.0	13.180252871829676	0.0
23	8.378788259641891E-4	0.0	0.0	13.632707437850337	0.0
24	8.378788259641891E-4	0.0	0.0	13.979589271799512	0.0
25	8.378788259641891E-4	0.0	0.0	14.268657466757158	0.0
26	8.378788259641891E-4	0.0	0.0	14.526724145154128	0.0
27	8.378788259641891E-4	0.0	0.0	14.808670370091077	0.0
28	8.378788259641891E-4	0.0	0.0	15.091035534441009	0.0
29	8.378788259641891E-4	0.0	0.0	15.396023427091974	0.0
30	8.378788259641891E-4	0.0	0.0	15.753378746365701	0.0
31	8.378788259641891E-4	0.0	0.0	16.049149971931058	0.0
32	8.378788259641891E-4	0.0	0.0	16.351624228104132	0.0
33	8.378788259641891E-4	0.0	0.0	16.63734090775792	0.0
34	8.378788259641891E-4	0.0	0.0	16.913840920326102	0.0
35	0.0012568182389462836	0.0	0.0	17.224275025345836	0.0
36	0.0012568182389462836	0.0	0.0	17.506221250282785	0.0
37	0.0012568182389462836	0.0	0.0	17.776437171656234	0.0
38	0.0012568182389462836	0.0	0.0	18.07346521546054	0.0
39	0.0012568182389462836	0.0	0.0	18.40777886702025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	20	7.0220424E-4	45.000004	1
AACCCCG	20	7.0220424E-4	45.000004	11
TAGAATA	20	7.0220424E-4	45.000004	11
ACGGGTC	20	7.0220424E-4	45.000004	5
GTATGCG	20	7.0220424E-4	45.000004	1
AGCGCAA	20	7.0220424E-4	45.000004	26
GCGATTA	30	2.158251E-6	45.000004	23
ACATCGG	20	7.0220424E-4	45.000004	2
GGCGATA	60	0.0	45.000004	8
AGGGCGC	30	2.158251E-6	45.000004	6
GCGTAAG	20	7.0220424E-4	45.000004	1
CTCGTAT	50	2.1827873E-11	45.0	42
ATAGGGT	35	1.2065175E-7	45.0	4
TCGTGTA	25	3.88149E-5	45.0	17
ATTCAAT	25	3.88149E-5	45.0	14
CAGATGA	25	3.88149E-5	45.0	34
TGATTGG	25	3.88149E-5	45.0	2
GTATACG	25	3.88149E-5	45.0	1
TAGGGAC	160	0.0	43.593754	5
TAGATGG	55	6.002665E-11	40.909092	2
>>END_MODULE