FastQCFastQC Report
Sat 18 Jun 2016
SRR3550181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190852
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC45310.38048388884596906No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC44340.3723384601948857No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG41350.34723038631164915No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC25710.21589584599933492No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.18356605186874608No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC17730.1488849999832053No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG253.8908904E-545.012
TACCGGT351.2119744E-745.040
ATAACGG403.4583718E-739.3752
ATCGGAT403.4583718E-739.37534
CTTAACG356.2484796E-638.5714261
TCGAACC356.2484796E-638.57142614
ACCGGAT301.1399468E-437.49999628
TCGATAG301.1399468E-437.4999961
CTAGCGG2100.037.4999962
CTAAGCG800.036.56251
TACGGCT6050.036.446287
CGTTTTT15150.036.0891081
TAACGCG250.002107138736.01
TATTGCG250.002107138736.01
AAACGCG250.002107138736.01
GTATCGA250.002107138736.09
TATCGGC250.002107138736.039
ATACCGG751.8189894E-1236.02
AGGGCGA9650.035.6735766
AAGGGCG3800.034.934215
Produced by FastQC (version 0.11.5)