##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550181_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1190852
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44855028164709	33.0	31.0	34.0	31.0	34.0
2	32.5497333001918	34.0	31.0	34.0	31.0	34.0
3	32.551498422977836	34.0	31.0	34.0	31.0	34.0
4	36.07945487768421	37.0	35.0	37.0	35.0	37.0
5	36.09630751764283	37.0	35.0	37.0	35.0	37.0
6	36.08119144948323	37.0	35.0	37.0	35.0	37.0
7	36.23126635383742	37.0	37.0	37.0	35.0	37.0
8	35.89663702962249	37.0	36.0	37.0	35.0	37.0
9	37.79814116279773	39.0	38.0	39.0	35.0	39.0
10	37.59223816225694	39.0	37.0	39.0	35.0	39.0
11	37.497291854907246	39.0	37.0	39.0	35.0	39.0
12	37.36876118946771	39.0	37.0	39.0	35.0	39.0
13	37.324012555716415	39.0	37.0	39.0	35.0	39.0
14	38.579750464373404	40.0	38.0	41.0	35.0	41.0
15	38.65893662688563	40.0	38.0	41.0	35.0	41.0
16	38.72111311901059	40.0	38.0	41.0	35.0	41.0
17	38.638887955850095	40.0	38.0	41.0	35.0	41.0
18	38.61906853244568	40.0	38.0	41.0	35.0	41.0
19	38.563445331577725	40.0	38.0	41.0	35.0	41.0
20	38.49249025067767	40.0	37.0	41.0	34.0	41.0
21	38.43415806498205	40.0	37.0	41.0	34.0	41.0
22	38.43552179447992	40.0	37.0	41.0	34.0	41.0
23	38.39240728486831	40.0	37.0	41.0	34.0	41.0
24	38.36177375526094	40.0	37.0	41.0	34.0	41.0
25	38.23992821945968	40.0	37.0	41.0	34.0	41.0
26	38.20371885003342	40.0	37.0	41.0	34.0	41.0
27	38.15218179924961	40.0	37.0	41.0	34.0	41.0
28	38.08172971956213	40.0	37.0	41.0	34.0	41.0
29	38.05588687763047	40.0	36.0	41.0	34.0	41.0
30	37.94483529439427	40.0	36.0	41.0	34.0	41.0
31	37.899566864732144	40.0	36.0	41.0	34.0	41.0
32	37.75557416034906	40.0	36.0	41.0	33.0	41.0
33	37.661186276716165	40.0	36.0	41.0	33.0	41.0
34	37.56890276877395	40.0	36.0	41.0	33.0	41.0
35	37.488670296560784	40.0	36.0	41.0	33.0	41.0
36	37.40677682869072	40.0	35.0	41.0	33.0	41.0
37	37.336365896013945	40.0	35.0	41.0	33.0	41.0
38	37.2465621252683	40.0	35.0	41.0	33.0	41.0
39	37.181121583538506	40.0	35.0	41.0	33.0	41.0
40	37.077616697960785	39.0	35.0	41.0	32.0	41.0
41	36.99659991333936	39.0	35.0	41.0	32.0	41.0
42	36.93893951557372	39.0	35.0	41.0	32.0	41.0
43	36.880644278214255	39.0	35.0	41.0	32.0	41.0
44	36.76881174150944	39.0	35.0	41.0	32.0	41.0
45	36.705834142277965	39.0	35.0	41.0	32.0	41.0
46	36.52742070383221	39.0	35.0	41.0	31.0	41.0
47	36.50510054985842	39.0	35.0	41.0	31.0	41.0
48	36.48412733068425	39.0	35.0	41.0	31.0	41.0
49	36.41256596117738	39.0	35.0	41.0	31.0	41.0
50	36.278735728705165	38.0	35.0	41.0	31.0	41.0
51	35.330977317080546	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	29.0
10	34.0
11	30.0
12	28.0
13	30.0
14	26.0
15	52.0
16	89.0
17	134.0
18	296.0
19	467.0
20	896.0
21	1493.0
22	2196.0
23	3151.0
24	4375.0
25	5879.0
26	7763.0
27	8986.0
28	9972.0
29	11531.0
30	13994.0
31	17707.0
32	23477.0
33	33500.0
34	68748.0
35	94690.0
36	80213.0
37	122463.0
38	226124.0
39	452067.0
40	396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24000631480654	18.782686681468395	22.28303769066181	14.694269313063252
2	29.251074020953066	26.547547470214607	26.278412430763858	17.922966078068477
3	31.834938346662728	25.329092112202016	26.036904669933797	16.79906487120146
4	28.626059325592095	25.36562058089502	27.505517058375013	18.50280303513787
5	27.539274401856822	29.239401705669554	24.32913577841747	18.892188114056154
6	28.089720636989313	34.70506830403778	22.881516762788323	14.32369429618458
7	80.59061915334567	6.141065388478165	8.977354028880164	4.290961429296
8	82.51008521629892	5.8969544494194075	7.2641268604326985	4.3288334738489755
9	76.02430864624655	7.48262588466073	9.242878208207234	7.250187260885483
10	44.93765808009728	22.97665872837263	16.953156227642058	15.13252696388804
11	32.95497677293232	25.402316996570523	23.128062933093283	18.514643297403875
12	29.10915882074347	23.166942659541238	27.101940459435763	20.62195806027953
13	26.08191446124287	23.70218969275779	29.724348617628387	20.491547228370948
14	21.89281287683104	26.76646636189888	28.696009243801917	22.644711517468167
15	21.005968835757926	25.806565383439757	32.65980995119461	20.52765582960771
16	24.38229099837763	24.714070262299597	29.698148888358922	21.20548985096385
17	24.47760091094443	25.542972594411395	28.16143399851535	21.81799249612882
18	24.80627315569021	25.26292100109837	27.847793008703015	22.083012834508402
19	25.37301024812487	26.835324624722467	26.433343522116935	21.35832160503572
20	27.589154655658305	26.058569830675854	27.035265507384633	19.31701000628122
21	26.284038654677495	27.744169720502633	26.657048902802366	19.314742722017513
22	25.354200186085258	24.787798987615588	26.896793220316212	22.961207605982946
23	24.44795826853379	26.77805470369114	26.978667374283287	21.795319653491784
24	24.576941551091153	24.742873169797758	27.89137525066087	22.788810028450218
25	23.54860217726468	27.559512013247655	26.53343992368489	22.35844588580277
26	23.01612626925932	27.543221155945492	27.265856714352417	22.174795860442774
27	23.14225445311424	28.22147504475787	26.90527454293229	21.7309959591956
28	21.96620570818204	27.891459224152122	28.115080631346295	22.027254436319545
29	23.376876387661945	26.964139960297334	27.44312475437754	22.215858897663185
30	23.019737129382996	28.32425859804577	26.809460789418	21.846543483153237
31	23.346645930812564	27.410627013264456	25.940083234524526	23.302643821398462
32	25.37309422161612	28.520840541058	24.84263367740072	21.263431559925163
33	24.291011813390746	28.082918784198203	25.35848283413892	22.267586568272126
34	23.2199299325189	27.41616926368684	27.026448290803557	22.337452512990698
35	23.81379046262676	28.197962467208352	25.549858420693756	22.438388649471136
36	22.97657475488138	30.369936818345185	25.52391061189804	21.1295778148754
37	23.871480251114328	28.922989590646026	26.225593104768684	20.97993705347096
38	23.947476260694025	28.62068502215221	25.341184294941772	22.090654422211998
39	24.947096700513583	26.79896410301196	25.921525092958653	22.332414103515802
40	23.813874436118006	27.135949723391317	27.17701276061173	21.873163079878942
41	22.03665946733935	27.296507038658035	26.683668499528068	23.983164994474546
42	22.709623026203086	27.582604723340935	27.128811976635212	22.578960273820762
43	24.360038023196836	26.211569531730223	26.626986392935475	22.80140605213746
44	22.803169495453677	26.85606607706079	26.98782048482935	23.352943942656182
45	22.477940163849077	26.145230473644084	26.70860862642881	24.668220736078034
46	23.46118577287522	27.925972329055167	25.855521928837504	22.75731996923211
47	21.915989560415568	27.43371972335773	28.725987780177554	21.92430293604915
48	22.625985428919797	26.317544077685557	27.754078592469927	23.302391900924714
49	22.522865981666907	25.592181060282893	28.802319683722246	23.08263327432796
50	22.177231091688977	26.279672033132577	27.717130256320683	23.82596661885776
51	21.711178215261008	26.02548427512403	26.729769946223374	25.53356756339159
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	306.0
1	964.5
2	1623.0
3	3280.5
4	4938.0
5	3614.0
6	2290.0
7	2219.0
8	2148.0
9	2169.0
10	2190.0
11	2313.5
12	2437.0
13	2532.0
14	2627.0
15	2598.0
16	2569.0
17	2652.5
18	2736.0
19	2884.5
20	3033.0
21	3487.0
22	3941.0
23	4263.5
24	4586.0
25	5648.0
26	8247.0
27	9784.0
28	12330.0
29	14876.0
30	17295.0
31	19714.0
32	23242.0
33	26770.0
34	29335.0
35	31900.0
36	34356.0
37	36812.0
38	41768.5
39	46725.0
40	50962.0
41	55199.0
42	60728.5
43	66258.0
44	83171.5
45	100085.0
46	100322.5
47	100560.0
48	97649.0
49	94738.0
50	87588.0
51	80438.0
52	74943.5
53	69449.0
54	67364.5
55	65280.0
56	61640.0
57	58000.0
58	57875.0
59	57750.0
60	56453.0
61	55156.0
62	50397.5
63	45639.0
64	39585.0
65	33531.0
66	29219.0
67	24907.0
68	21637.5
69	18368.0
70	15616.0
71	12864.0
72	11226.0
73	9588.0
74	7662.5
75	4832.5
76	3928.0
77	3244.0
78	2560.0
79	1888.5
80	1217.0
81	816.0
82	415.0
83	359.0
84	303.0
85	177.0
86	51.0
87	73.0
88	95.0
89	53.0
90	11.0
91	7.5
92	4.0
93	2.5
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1190852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.766714650093338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7595749030184	17.29256054782131
2	9.672650258473826	4.59774237206598
3	3.463724959400026	2.4696408820939966
4	1.7901851717924233	1.701872805952754
5	1.1019943884566297	1.3095393088226417
6	0.7883050144563961	1.124125220149372
7	0.5939308336862642	0.988104925227944
8	0.5031566623192903	0.9566704654108755
9	0.41989979173514097	0.8981674678622443
>10	7.661369826105771	47.18063567279798
>50	1.1933989702205798	17.454729784780813
>100	0.04680695078574237	1.875280678092384
>500	0.0025011347748103013	0.38672409582176137
>1k	0.0025011347748103013	1.7642057730999325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC	4531	0.38048388884596906	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	4434	0.3723384601948857	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG	4135	0.34723038631164915	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	2571	0.21589584599933492	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2186	0.18356605186874608	No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	1773	0.1488849999832053	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10110408346293243	0.0
2	0.0	0.0	0.0	0.5779055667706818	0.0
3	0.0	0.0	0.0	0.7888469767863681	0.0
4	0.0	0.0	0.0	1.095938034281338	0.0
5	0.0	0.0	0.0	2.1251171430202915	0.0
6	0.0	0.0	0.0	2.7414825687826867	0.0
7	0.0	0.0	0.0	3.265393180680723	0.0
8	0.0	0.0	0.0	4.085478296211452	0.0
9	0.0	0.0	0.0	4.417677427589658	0.0
10	0.0	0.0	0.0	5.440222630519997	0.0
11	0.0	0.0	0.0	6.227726031446393	0.0
12	0.0	0.0	0.0	7.29880791231824	0.0
13	0.0	0.0	0.0	7.592547184704732	0.0
14	0.0	0.0	0.0	7.719767023945881	0.0
15	0.0	0.0	0.0	7.968748425497039	0.0
16	0.0	0.0	0.0	8.37501217615623	0.0
17	0.0	0.0	0.0	8.869867960082361	0.0
18	0.0	0.0	0.0	9.415947573669945	0.0
19	0.0	0.0	0.0	9.832624037243923	0.0
20	0.0	0.0	0.0	10.192954288190304	0.0
21	0.0	0.0	0.0	10.621303067047794	0.0
22	0.0	0.0	0.0	11.080889984649646	0.0
23	0.0	0.0	0.0	11.52468988589682	0.0
24	0.0	0.0	0.0	11.899547550829155	0.0
25	0.0	0.0	0.0	12.22494482941625	0.0
26	0.0	0.0	0.0	12.53203588691122	0.0
27	8.397349124828274E-5	0.0	0.0	12.84693647909228	0.0
28	8.397349124828274E-5	0.0	0.0	13.16234091222083	0.0
29	8.397349124828274E-5	0.0	0.0	13.497563089283975	0.0
30	8.397349124828274E-5	0.0	0.0	13.887368035658504	0.0
31	1.6794698249656548E-4	0.0	0.0	14.256851397150948	0.0
32	1.6794698249656548E-4	0.0	0.0	14.619280985378536	0.0
33	1.6794698249656548E-4	0.0	0.0	14.959289651442832	0.0
34	2.5192047374484824E-4	0.0	0.0	15.295015669453466	0.0
35	2.5192047374484824E-4	0.0	0.0	15.650391484416199	0.0
36	2.5192047374484824E-4	0.0	0.0	16.01676782673246	0.0
37	2.5192047374484824E-4	0.0	0.0	16.39565621924471	0.0
38	2.5192047374484824E-4	0.0	0.0	16.758589648419786	0.0
39	2.5192047374484824E-4	0.0	0.0	17.127821089438488	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAACG	25	3.8908904E-5	45.0	12
TACCGGT	35	1.2119744E-7	45.0	40
ATAACGG	40	3.4583718E-7	39.375	2
ATCGGAT	40	3.4583718E-7	39.375	34
CTTAACG	35	6.2484796E-6	38.571426	1
TCGAACC	35	6.2484796E-6	38.571426	14
ACCGGAT	30	1.1399468E-4	37.499996	28
TCGATAG	30	1.1399468E-4	37.499996	1
CTAGCGG	210	0.0	37.499996	2
CTAAGCG	80	0.0	36.5625	1
TACGGCT	605	0.0	36.44628	7
CGTTTTT	1515	0.0	36.089108	1
TAACGCG	25	0.0021071387	36.0	1
TATTGCG	25	0.0021071387	36.0	1
AAACGCG	25	0.0021071387	36.0	1
GTATCGA	25	0.0021071387	36.0	9
TATCGGC	25	0.0021071387	36.0	39
ATACCGG	75	1.8189894E-12	36.0	2
AGGGCGA	965	0.0	35.673576	6
AAGGGCG	380	0.0	34.93421	5
>>END_MODULE