FastQCFastQC Report
Sat 18 Jun 2016
SRR3550180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244313
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC34970.2810386132749557No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC34790.27959203190837034No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG31230.25098186710256987No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC26710.2146566016749805No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21400.1719824513607107No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC19960.16040980042802735No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG207.033511E-445.00000437
ATTCGAC253.890993E-545.025
TATGCGG2050.041.7073172
TCGATAG403.4585355E-739.3750041
TTACCGG403.4585355E-739.3750042
TACGTAG403.4585355E-739.3750041
TCGTCCC1700.038.3823538
CGTTTTT15550.038.344051
TATAGCG301.13997594E-437.5000041
TATCGAG301.13997594E-437.5000041
AACGTAG504.879439E-836.01
ATTCGCG250.002107175536.01
TTATCCG250.002107175536.033
ACCTACG250.002107175536.029
TCGCTTA250.002107175536.035
TTGCGAC250.002107175536.031
TTACGAG250.002107175536.01
CCGGTCA250.002107175536.032
GTGCCGA250.002107175536.016
ACGGGTA1400.035.3571435
Produced by FastQC (version 0.11.5)