FastQCFastQC Report
Sat 18 Jun 2016
SRR3550177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215651
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC69540.5720391790077909TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT47790.3931226972214887TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC42410.3488665743704402TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC29060.23904887175677886TruSeq Adapter, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC19860.16336925647245798TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17880.14708168709604977No Hit
AGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG15490.12742143921240554No Hit
ACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG13960.11483559014881738No Hit
GCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG13410.11031126532203732TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC13000.10693858681480128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC207.033448E-445.012
GTCGACA207.033448E-445.011
CGACATA207.033448E-445.013
TGATTCG253.89094E-545.015
CGTAGCG207.033448E-445.02
GTATACG351.2120108E-745.01
TATAGCG800.045.01
ACGTATG453.8562575E-1045.01
CGGTCTA351.2120108E-745.031
ATAACGC207.033448E-445.011
CGGTCGA207.033448E-445.043
CGTCCCG207.033448E-445.033
TATCGTG453.8562575E-1045.01
ATCGATT207.033448E-445.020
CGTTAGG1300.041.538462
AACGTTG451.9284926E-840.01
TTACGAG451.9284926E-840.01
CATGCGG750.039.02
CACAACG1050.038.5714312
GCATAAC356.2485888E-638.5714269
Produced by FastQC (version 0.11.5)