##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1215651
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.847619917229533	31.0	31.0	34.0	30.0	34.0
2	31.99770657861508	33.0	31.0	34.0	30.0	34.0
3	32.055071727000595	33.0	31.0	34.0	30.0	34.0
4	35.66714213207574	37.0	35.0	37.0	33.0	37.0
5	35.67932655013651	37.0	35.0	37.0	33.0	37.0
6	35.67799310821938	37.0	35.0	37.0	33.0	37.0
7	35.91610914645733	37.0	35.0	37.0	35.0	37.0
8	35.867981846763584	37.0	35.0	37.0	35.0	37.0
9	37.453882734436114	39.0	37.0	39.0	35.0	39.0
10	37.12229579048592	39.0	37.0	39.0	33.0	39.0
11	37.05128527842284	39.0	37.0	39.0	33.0	39.0
12	36.89192292853788	39.0	37.0	39.0	33.0	39.0
13	36.82985824056411	39.0	37.0	39.0	33.0	39.0
14	37.85014284527385	40.0	37.0	41.0	33.0	41.0
15	37.928236804806644	40.0	37.0	41.0	33.0	41.0
16	37.98808457361529	40.0	37.0	41.0	33.0	41.0
17	37.99354008675187	40.0	37.0	41.0	33.0	41.0
18	37.90534454378765	40.0	37.0	41.0	33.0	41.0
19	37.86743892778437	40.0	37.0	41.0	33.0	41.0
20	37.736031969701834	39.0	36.0	41.0	33.0	41.0
21	37.61782287844127	39.0	36.0	41.0	33.0	41.0
22	37.5898765352885	39.0	36.0	41.0	33.0	41.0
23	37.590168559890955	39.0	36.0	41.0	33.0	41.0
24	37.63204077486055	39.0	36.0	41.0	33.0	41.0
25	37.590164446868386	39.0	36.0	41.0	33.0	41.0
26	37.54190964347498	39.0	36.0	41.0	33.0	41.0
27	37.48015178698492	39.0	36.0	41.0	33.0	41.0
28	37.3583512044164	39.0	36.0	41.0	32.0	41.0
29	37.33524588882829	39.0	36.0	41.0	32.0	41.0
30	37.250639369358474	39.0	36.0	41.0	32.0	41.0
31	37.16102236579413	39.0	35.0	41.0	32.0	41.0
32	37.09431572054808	39.0	35.0	41.0	31.0	41.0
33	37.04080365170596	39.0	35.0	41.0	31.0	41.0
34	36.905393900058485	39.0	35.0	41.0	31.0	41.0
35	36.90315148015343	39.0	35.0	41.0	31.0	41.0
36	36.82296234692358	39.0	35.0	41.0	31.0	41.0
37	36.73121973329516	39.0	35.0	41.0	31.0	41.0
38	36.63488534126982	39.0	35.0	40.0	30.0	41.0
39	36.60290330037157	39.0	35.0	40.0	30.0	41.0
40	36.494765356175414	39.0	35.0	40.0	30.0	41.0
41	36.413564419393396	39.0	35.0	40.0	30.0	41.0
42	36.299064451886274	38.0	35.0	40.0	30.0	41.0
43	36.21942728628529	38.0	35.0	40.0	30.0	41.0
44	36.124362995629504	38.0	35.0	40.0	30.0	41.0
45	36.068271239031596	38.0	35.0	40.0	30.0	41.0
46	36.04495862710597	38.0	35.0	40.0	30.0	41.0
47	35.98796036033368	38.0	35.0	40.0	30.0	41.0
48	35.97016824730124	38.0	35.0	40.0	30.0	41.0
49	35.90231242354919	38.0	35.0	40.0	29.0	41.0
50	35.76877574238001	38.0	35.0	40.0	29.0	41.0
51	34.41702758439717	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	29.0
10	34.0
11	39.0
12	15.0
13	14.0
14	35.0
15	32.0
16	76.0
17	138.0
18	315.0
19	509.0
20	879.0
21	1512.0
22	2180.0
23	3308.0
24	4968.0
25	6908.0
26	9004.0
27	11553.0
28	14157.0
29	17904.0
30	22216.0
31	28704.0
32	37195.0
33	49950.0
34	82171.0
35	99348.0
36	104467.0
37	148457.0
38	247704.0
39	321725.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.8338412916207	23.046746146714806	26.056327021488894	10.063085540175592
2	29.663447815203543	25.228869140896528	26.90640652621517	18.201276517684764
3	30.591674748756013	24.783428796587177	27.896328798314645	16.728567656342157
4	28.63066784792675	26.03222470922987	26.712107340017816	18.625000102825563
5	25.620840191798465	29.395278743652575	25.065499884424064	19.91838118012489
6	27.05455759917937	34.15347003375147	23.69874248447951	15.093229882589657
7	78.89188591133475	6.188124716715571	9.186764951453995	5.733224420495684
8	77.94556167847514	5.425817113628829	9.519508477350819	7.109112730545197
9	71.64424658063868	7.583179711940351	12.419189389059854	8.353384318361108
10	38.1310096401023	27.371178076602575	18.74814399856538	15.749668284729745
11	29.106297777898426	24.57251299920783	25.66057198982274	20.660617233071005
12	26.430447554437908	22.36636995321848	30.33099137828209	20.87219111406152
13	24.92861849330112	23.61129962464556	31.809540731673813	19.650541150379507
14	19.896664420956345	28.003596426935033	29.40572582097987	22.694013331128755
15	18.795772799923665	25.68837602239459	34.047929874610396	21.467921303071357
16	21.048804303208733	26.09704594492992	30.15882025351026	22.69532949835109
17	21.225006190098966	25.812095741294172	28.02235180985332	24.94054625875354
18	21.280531994791268	26.05772544916263	30.249635791851443	22.412106764194657
19	22.330915698666807	27.872144225604224	27.34946131743403	22.447478758294938
20	23.459446831368542	27.42193277511391	27.254121454266066	21.86449893925148
21	22.758587785474614	28.243550163657165	28.23491281626059	20.762949234607632
22	21.63351159173151	26.38438170165615	25.696931109339772	26.28517559727257
23	21.37118301222966	26.93733645594007	27.296238805380817	24.39524172644945
24	22.45134499951055	24.726340043318356	28.16367526535165	24.658639691819445
25	21.419716678553304	26.571030665873675	26.015772618950667	25.99348003662235
26	20.063570876838828	27.076027576993727	27.12266925293526	25.737732293232185
27	22.326967196999796	26.10667041774325	26.011741856832266	25.55462052842469
28	18.629524427652346	27.119872397587798	27.42481189091277	26.82579128384709
29	20.376571894400612	24.70174416835095	25.812671564453943	29.109012372794496
30	21.908426020296943	25.726298090488143	25.619935326833108	26.745340562381802
31	21.126540429777954	27.377841173165653	24.642845685151414	26.852772711904983
32	21.047981698694773	26.66028325563834	24.360774597314524	27.930960448352366
33	21.732882217017877	25.556923821063776	25.92503934106088	26.785154620857465
34	19.544754209884253	24.930921785940207	29.5162016072047	26.00812239697084
35	19.200658741694777	23.234382236349084	28.33255597206764	29.232403049888493
36	21.781004581084538	25.995701068810046	27.771128391289935	24.452165958815485
37	20.727824021861537	26.277443114841347	29.722593079757264	23.272139783539846
38	20.359543980961643	26.749535845403	28.358961576965758	24.531958596669604
39	23.57839544408716	23.339428832781778	29.66649145190519	23.41568427122587
40	22.082818177256467	21.57214529499009	33.1839483535982	23.161088174155246
41	20.194940817718244	24.16507698344344	29.29072570992826	26.349256488910054
42	22.301301936164244	23.97744089380916	28.10395417763815	25.617302992388442
43	22.984804026813617	23.065830571438678	27.75903610493472	26.19032929681298
44	20.8869979953128	24.18375010591033	27.10407839091976	27.82517350785711
45	20.804737543916797	22.003848143916304	28.217309079661845	28.974105232505053
46	22.305826260991026	23.156563849328467	29.132292080539564	25.405317809140946
47	19.438473706680618	22.907890504758356	31.816779651396658	25.836856137164364
48	19.838917584076352	21.785611166362713	30.355258211443907	28.020213038117024
49	22.53294736729538	20.528918250386006	31.332512374028404	25.605622008290208
50	19.781664309904734	20.567580662542127	31.85387911497626	27.796875912576883
51	19.726138505212436	20.26420411779368	28.82718806631179	31.182469310682094
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	375.0
1	787.5
2	1200.0
3	2169.5
4	3139.0
5	2216.0
6	1293.0
7	1259.0
8	1225.0
9	1367.5
10	1510.0
11	1579.5
12	1649.0
13	1813.5
14	1978.0
15	1902.0
16	1826.0
17	2073.5
18	2321.0
19	2328.5
20	2336.0
21	2903.0
22	3470.0
23	3462.5
24	3455.0
25	4323.0
26	6692.5
27	8194.0
28	9967.5
29	11741.0
30	13515.0
31	15289.0
32	17768.5
33	20248.0
34	23231.5
35	26215.0
36	28402.5
37	30590.0
38	34426.0
39	38262.0
40	42506.5
41	46751.0
42	53982.0
43	61213.0
44	71787.5
45	82362.0
46	102239.5
47	122117.0
48	140183.5
49	158250.0
50	151460.5
51	144671.0
52	120895.0
53	97119.0
54	82157.5
55	67196.0
56	60952.0
57	54708.0
58	51845.0
59	48982.0
60	46163.0
61	43344.0
62	38386.0
63	33428.0
64	28876.5
65	24325.0
66	20904.0
67	17483.0
68	14094.0
69	10705.0
70	9578.0
71	8451.0
72	7284.0
73	6117.0
74	4571.0
75	2417.0
76	1809.0
77	1307.5
78	806.0
79	765.5
80	725.0
81	478.0
82	231.0
83	210.0
84	189.0
85	133.0
86	77.0
87	51.5
88	26.0
89	24.0
90	22.0
91	14.0
92	6.0
93	5.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1215651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.668819196316498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.50963743927218	20.21428047344323
2	12.320891343464643	7.064508125264979
3	4.984818310164633	4.287265645817934
4	2.500665182259489	2.8676447192288457
5	1.5039735513047656	2.155857290919918
6	0.9963541222955042	1.7138577712556657
7	0.6976810571617763	1.4001184459126146
8	0.5298653870104404	1.2152491982870868
9	0.4172715734064355	1.0766414964376447
>10	4.410316901009056	29.585943373134622
>50	1.0126530639241116	19.423569289214097
>100	0.10599825836084056	5.344953533779558
>500	0.006388936119290555	1.1192939968047344
>1k	0.0031944680596452777	1.9518542443536295
>5k	2.9040618724047974E-4	0.5789623961455016
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	6954	0.5720391790077909	TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	4779	0.3931226972214887	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	4241	0.3488665743704402	TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	2906	0.23904887175677886	TruSeq Adapter, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1986	0.16336925647245798	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1788	0.14708168709604977	No Hit
AGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG	1549	0.12742143921240554	No Hit
ACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG	1396	0.11483559014881738	No Hit
GCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG	1341	0.11031126532203732	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC	1300	0.10693858681480128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.93562708376006E-4	0.0	0.0	0.42981085854410517	0.0
2	4.93562708376006E-4	0.0	0.0	1.8672299862378265	0.0
3	4.93562708376006E-4	0.0	0.0	2.743879616765009	0.0
4	4.93562708376006E-4	0.0	0.0	3.713730338723861	0.0
5	4.93562708376006E-4	0.0	0.0	6.144197635670106	0.0
6	4.93562708376006E-4	0.0	0.0	7.543036611659103	0.0
7	5.75823159772007E-4	0.0	0.0	8.973463600984164	0.0
8	5.75823159772007E-4	0.0	0.0	11.529131304955122	0.0
9	5.75823159772007E-4	0.0	0.0	12.50975814604685	0.0
10	5.75823159772007E-4	0.0	0.0	14.39154823218177	0.0
11	6.58083611168008E-4	0.0	0.0	16.78384667968027	0.0
12	6.58083611168008E-4	0.0	0.0	18.583376314419187	0.0
13	6.58083611168008E-4	0.0	0.0	19.29920676246719	0.0
14	6.58083611168008E-4	0.0	0.0	19.568856522143278	0.0
15	6.58083611168008E-4	0.0	0.0	20.088989356320194	0.0
16	6.58083611168008E-4	0.0	0.0	21.11658691515904	0.0
17	6.58083611168008E-4	0.0	0.0	22.407171137110897	0.0
18	6.58083611168008E-4	0.0	0.0	23.857587416125188	0.0
19	6.58083611168008E-4	0.0	0.0	24.675749865709815	0.0
20	6.58083611168008E-4	0.0	0.0	25.463804990083503	0.0
21	6.58083611168008E-4	0.0	0.0	26.39918858290743	0.0
22	6.58083611168008E-4	0.0	0.0	27.367311835386964	0.0
23	6.58083611168008E-4	0.0	0.0	28.335352827415104	0.0
24	6.58083611168008E-4	0.0	0.0	29.093629668383443	0.0
25	6.58083611168008E-4	0.0	0.0	29.763970086809454	0.0
26	6.58083611168008E-4	0.0	0.0	30.39433192585701	0.0
27	6.58083611168008E-4	0.0	0.0	30.98117798611608	0.0
28	6.58083611168008E-4	0.0	0.0	31.58126797904991	0.0
29	6.58083611168008E-4	0.0	0.0	32.22569635528618	0.0
30	6.58083611168008E-4	0.0	0.0	32.92301820177008	0.0
31	6.58083611168008E-4	0.0	0.0	33.565307806270056	0.0
32	6.58083611168008E-4	0.0	0.0	34.16128477663408	0.0
33	6.58083611168008E-4	0.0	0.0	34.74681466967082	0.0
34	6.58083611168008E-4	0.0	0.0	35.34394328635439	0.0
35	7.403440625640089E-4	0.0	0.0	35.95925146279648	0.0
36	8.226045139600099E-4	0.0	0.0	36.57094017937714	0.0
37	8.226045139600099E-4	0.0	0.0	37.16395577349091	0.0
38	8.226045139600099E-4	0.0	0.0	37.743398393124345	0.0
39	8.226045139600099E-4	0.0	0.0	38.33707207084928	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGC	20	7.033448E-4	45.0	12
GTCGACA	20	7.033448E-4	45.0	11
CGACATA	20	7.033448E-4	45.0	13
TGATTCG	25	3.89094E-5	45.0	15
CGTAGCG	20	7.033448E-4	45.0	2
GTATACG	35	1.2120108E-7	45.0	1
TATAGCG	80	0.0	45.0	1
ACGTATG	45	3.8562575E-10	45.0	1
CGGTCTA	35	1.2120108E-7	45.0	31
ATAACGC	20	7.033448E-4	45.0	11
CGGTCGA	20	7.033448E-4	45.0	43
CGTCCCG	20	7.033448E-4	45.0	33
TATCGTG	45	3.8562575E-10	45.0	1
ATCGATT	20	7.033448E-4	45.0	20
CGTTAGG	130	0.0	41.53846	2
AACGTTG	45	1.9284926E-8	40.0	1
TTACGAG	45	1.9284926E-8	40.0	1
CATGCGG	75	0.0	39.0	2
CACAACG	105	0.0	38.57143	12
GCATAAC	35	6.2485888E-6	38.571426	9
>>END_MODULE