SRR3550176_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1643288
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	10190	0.6200982420610386	RNA PCR Primer, Index 40 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	8907	0.5420230659507037	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	6655	0.40498074591915717	Illumina PCR Primer Index 8 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	3013	0.18335191396760642	RNA PCR Primer, Index 40 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	2993	0.1821348418536495	RNA PCR Primer, Index 40 (96% over 27bp)
AGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	2214	0.13472988301502842	Illumina PCR Primer Index 8 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC	2208	0.13436476138084133	No Hit
GCCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	2136	0.12998330177059653	RNA PCR Primer, Index 40 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2011	0.1223766010583659	No Hit
ACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	1796	0.10929307583332928	RNA PCR Primer, Index 40 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	1697	0.10326856886924264	Illumina PCR Primer Index 8 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	1680	0.10223405757237927	Illumina PCR Primer Index 8 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.034172E-4	45.000004	40
CGCGATT	25	3.8915394E-5	45.000004	30
CGTTATC	50	2.1827873E-11	45.000004	42
ACGATAG	30	2.1660726E-6	45.000004	1
CCGTACT	25	3.8915394E-5	45.000004	17
AATTGCG	20	7.034172E-4	45.000004	1
TTGCGAC	25	3.8915394E-5	45.000004	1
GCGATCG	20	7.034172E-4	45.000004	9
CGTAATA	20	7.034172E-4	45.000004	32
ACGTATG	20	7.034172E-4	45.000004	1
TCGCAAA	45	3.8562575E-10	45.0	12
ACGTTAG	45	3.8562575E-10	45.0	1
CGGTCTA	75	0.0	45.0	31
TATCTCG	65	0.0	44.999996	1
AGTCGAG	65	0.0	44.999996	1
TTAACGG	165	0.0	42.272728	2
GTTAACG	155	0.0	40.64516	1
GCGCGTT	45	1.9292202E-8	40.0	1
TGTTACG	45	1.9292202E-8	40.0	1
GCTAGCG	125	0.0	39.6	1