##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1643288
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89856921002283	33.0	31.0	34.0	30.0	34.0
2	32.051320888365275	33.0	31.0	34.0	30.0	34.0
3	32.107829546616294	33.0	31.0	34.0	30.0	34.0
4	35.70989321409272	37.0	35.0	37.0	33.0	37.0
5	35.72905053770246	37.0	35.0	37.0	35.0	37.0
6	35.72519850446179	37.0	35.0	37.0	33.0	37.0
7	35.944031113231524	37.0	35.0	37.0	35.0	37.0
8	35.90318739015924	37.0	35.0	37.0	35.0	37.0
9	37.48789074100218	39.0	37.0	39.0	35.0	39.0
10	37.205868356611866	39.0	37.0	39.0	34.0	39.0
11	37.1579364055479	39.0	37.0	39.0	34.0	39.0
12	37.01075648334315	39.0	37.0	39.0	33.0	39.0
13	36.94686689125704	39.0	37.0	39.0	33.0	39.0
14	37.988877786486604	40.0	37.0	41.0	33.0	41.0
15	38.055978014809334	40.0	37.0	41.0	33.0	41.0
16	38.1231470077065	40.0	37.0	41.0	33.0	41.0
17	38.14265484808506	40.0	37.0	41.0	33.0	41.0
18	38.05270348228673	40.0	37.0	41.0	33.0	41.0
19	38.016704314764056	40.0	37.0	41.0	33.0	41.0
20	37.89505369722167	40.0	37.0	41.0	33.0	41.0
21	37.790309428414254	39.0	36.0	41.0	33.0	41.0
22	37.76286445224452	39.0	36.0	41.0	33.0	41.0
23	37.77320287131653	39.0	36.0	41.0	33.0	41.0
24	37.84135221580149	40.0	36.0	41.0	33.0	41.0
25	37.775071077011454	40.0	36.0	41.0	33.0	41.0
26	37.738319150386296	40.0	36.0	41.0	33.0	41.0
27	37.67349667252484	40.0	36.0	41.0	33.0	41.0
28	37.573141165760354	39.0	36.0	41.0	33.0	41.0
29	37.534746800317414	39.0	36.0	41.0	33.0	41.0
30	37.406172868054774	39.0	36.0	41.0	32.0	41.0
31	37.303732516759084	39.0	36.0	41.0	32.0	41.0
32	37.25046978983599	39.0	36.0	41.0	32.0	41.0
33	37.18670799032184	39.0	35.0	41.0	32.0	41.0
34	37.073758829858185	39.0	35.0	41.0	31.0	41.0
35	37.04227560841435	39.0	35.0	41.0	31.0	41.0
36	36.942535331603466	39.0	35.0	41.0	31.0	41.0
37	36.85860421301683	39.0	35.0	41.0	31.0	41.0
38	36.756925748864475	39.0	35.0	41.0	31.0	41.0
39	36.6875416847199	39.0	35.0	40.0	31.0	41.0
40	36.55055230732531	39.0	35.0	40.0	30.0	41.0
41	36.51325269824888	39.0	35.0	40.0	30.0	41.0
42	36.43755689812133	39.0	35.0	40.0	30.0	41.0
43	36.35998072157771	38.0	35.0	40.0	30.0	41.0
44	36.2615676619071	38.0	35.0	40.0	30.0	41.0
45	36.13656461922682	38.0	35.0	40.0	30.0	41.0
46	36.06958488104337	38.0	35.0	40.0	30.0	41.0
47	35.9972402889816	38.0	35.0	40.0	30.0	41.0
48	36.024825228444435	38.0	35.0	40.0	30.0	41.0
49	35.98011912701851	38.0	35.0	40.0	30.0	41.0
50	35.836856351412536	38.0	35.0	40.0	29.0	41.0
51	34.475438876204294	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	20.0
9	44.0
10	59.0
11	41.0
12	23.0
13	19.0
14	28.0
15	40.0
16	92.0
17	191.0
18	351.0
19	629.0
20	1084.0
21	1765.0
22	2766.0
23	4137.0
24	6053.0
25	8422.0
26	11012.0
27	13986.0
28	17518.0
29	22520.0
30	28456.0
31	37083.0
32	48269.0
33	64923.0
34	104477.0
35	133801.0
36	141586.0
37	204645.0
38	341954.0
39	447155.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.09492675660018	22.445365632804474	25.917002984260822	10.542704626334519
2	30.36960045956643	25.46985068959306	26.476734449469603	17.68381440137091
3	31.25343822872193	24.320265224355072	27.142898871043908	17.28339767587909
4	28.709209828100736	25.974753056068078	26.511360151111674	18.804676964719512
5	24.97912721324564	29.65554425030792	25.03523423769905	20.33009429874739
6	26.078995282628487	34.103395144369095	24.49509763352498	15.322511939477437
7	77.97689753713287	6.395105422786511	9.52790989771726	6.10008714236336
8	76.78215869646708	5.509746313488567	10.13614168666722	7.571953303377131
9	70.10171071656337	7.791695673552049	13.468302573864108	8.638291036020467
10	37.30575529061248	26.806682699563318	19.354124170565353	16.533437839258852
11	28.079314155522344	24.415927092512085	26.281942057630797	21.222816694334774
12	25.023854613433556	22.785476435049727	30.92592412285613	21.264744828660586
13	24.245111021318237	23.92362142241652	32.45420157635181	19.37706597991344
14	19.830973024813666	27.889511759350764	29.768610249694515	22.510904966141055
15	18.050213961277635	25.514091260935395	35.10023805930549	21.33545671848148
16	20.079681711300758	26.024409598317522	30.59993135713277	23.29597733324895
17	20.08016854014634	26.107048794855192	27.704638505240712	26.10814415975775
18	20.45794772431856	25.947551494321143	30.693828470724547	22.90067231063575
19	21.648609373402593	27.157747150834183	27.72861482588566	23.46502864987756
20	22.863734171977157	27.233814157956488	27.557068511423438	22.345383158642914
21	22.184242810755023	28.404028995526044	28.005681292627948	21.406046901090985
22	20.732640900438636	26.173987761122824	25.658557720862074	27.43481361757647
23	20.238631329383526	27.376150741683748	27.465118713213993	24.92009921571873
24	21.62104269002147	24.592767670670025	28.868889689451883	24.917299949856627
25	20.781749760236792	27.26332815671994	25.99605181806232	25.958870264980945
26	19.282864598293177	26.959668664287694	28.70622800142154	25.051238735997583
27	21.92007730842068	26.514828806636455	27.10809060858474	24.45700327635813
28	18.748874208294588	27.653704037271616	27.879410060804922	25.718011693628874
29	21.416209453242523	25.242927593945797	27.47029126969831	25.87057168311337
30	22.24844336476625	27.61652248419023	25.703528535472785	24.431505615570735
31	21.151922243696784	30.58143186100063	23.44786793307077	24.81877796223182
32	22.534881286786003	27.842532775752026	24.23999931843962	25.38258661902235
33	21.104091309618276	28.839436544294124	22.754258535326734	27.302213610760866
34	19.405119492140148	28.423380442137958	26.43894436033124	25.73255570539066
35	20.656026210865047	25.520359182322274	25.544152942150127	28.279461664662552
36	19.888114560563945	29.463490270725522	24.575241832229043	26.073153336481493
37	20.469753323823944	29.92859437907415	25.144770727955173	24.456881569146734
38	20.54411642998671	29.28993578727527	23.955812979830682	26.21013480290734
39	22.13543821898535	27.626989304370262	24.671086261203147	25.566486215441238
40	21.574002852817035	26.078204185754416	28.2414890147071	24.106303946721454
41	18.358254913320124	26.10662281961531	25.14738743300018	30.38773483406439
42	20.56730165375759	24.078798116946025	27.0979280564332	28.255972172863185
43	22.180104765567567	23.330177059651138	27.35874661045416	27.130971564327126
44	20.90662135912877	25.147265725788788	26.595459834186098	27.350653080896347
45	21.68086178442245	24.062489350619003	26.812950620950193	27.443698244008353
46	22.604071836464453	25.53983233614558	26.92133089269805	24.934764934691913
47	19.04730029063682	24.74277180871521	29.398316059023127	26.81161184162484
48	19.07304136584701	22.95209360745043	28.993517873921064	28.981347152781495
49	21.729788083403516	21.947522284590406	30.09971471829649	26.222974913709585
50	19.85963507309735	21.902490616374003	29.966688736240997	28.27118557428765
51	19.299173364620202	21.80147363091558	26.985714007526372	31.91363899693785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	508.0
1	1083.0
2	1658.0
3	2821.5
4	3985.0
5	2894.0
6	1803.0
7	1781.0
8	1759.0
9	1858.0
10	1957.0
11	2159.5
12	2362.0
13	2453.5
14	2545.0
15	2509.0
16	2473.0
17	2732.5
18	2992.0
19	3008.0
20	3024.0
21	3549.0
22	4074.0
23	4284.5
24	4495.0
25	6286.5
26	9723.5
27	11369.0
28	13190.5
29	15012.0
30	17582.0
31	20152.0
32	24045.0
33	27938.0
34	30253.0
35	32568.0
36	38113.0
37	43658.0
38	46138.5
39	48619.0
40	58291.5
41	67964.0
42	76930.5
43	85897.0
44	102154.5
45	118412.0
46	146015.5
47	173619.0
48	199934.5
49	226250.0
50	215676.0
51	205102.0
52	164778.0
53	124454.0
54	103966.5
55	83479.0
56	74396.0
57	65313.0
58	61660.0
59	58007.0
60	55095.5
61	52184.0
62	46827.0
63	41470.0
64	36317.0
65	31164.0
66	26870.5
67	22577.0
68	19632.0
69	16687.0
70	14018.5
71	11350.0
72	9461.5
73	7573.0
74	6351.0
75	4057.0
76	2985.0
77	2350.5
78	1716.0
79	1082.0
80	448.0
81	371.0
82	294.0
83	188.0
84	82.0
85	68.0
86	54.0
87	41.5
88	29.0
89	18.0
90	7.0
91	6.5
92	6.0
93	5.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1643288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.74464646456038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.82792638973831	16.536334124737394
2	13.146484449411325	6.506102199050479
3	5.871792456341293	4.358862853363137
4	3.110821038384896	3.0790466723740346
5	1.951206591158465	2.414095863876811
6	1.2519217035664698	1.858701597363747
7	0.9057884741045947	1.568939089437426
8	0.6636726177889061	1.3137875436316626
9	0.5089589742185932	1.1334608893803948
>10	4.180985652821242	23.21246892194201
>50	1.2464588046817462	21.946255248629583
>100	0.31905067953934185	11.109246782533928
>500	0.010452517771408496	1.731928531419737
>1k	0.00373304206121732	1.6449133481280256
>5k	4.977389414956427E-4	0.9583370717520248
>10k+	2.4886947074782134E-4	0.6275192623797156
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	10190	0.6200982420610386	RNA PCR Primer, Index 40 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	8907	0.5420230659507037	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	6655	0.40498074591915717	Illumina PCR Primer Index 8 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	3013	0.18335191396760642	RNA PCR Primer, Index 40 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	2993	0.1821348418536495	RNA PCR Primer, Index 40 (96% over 27bp)
AGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	2214	0.13472988301502842	Illumina PCR Primer Index 8 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC	2208	0.13436476138084133	No Hit
GCCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	2136	0.12998330177059653	RNA PCR Primer, Index 40 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2011	0.1223766010583659	No Hit
ACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	1796	0.10929307583332928	RNA PCR Primer, Index 40 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	1697	0.10326856886924264	Illumina PCR Primer Index 8 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	1680	0.10223405757237927	Illumina PCR Primer Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4341442279137923E-4	0.0	0.0	0.6011727706890089	0.0
2	2.4341442279137923E-4	0.0	0.0	2.136996071291216	0.0
3	2.4341442279137923E-4	0.0	0.0	3.0920934127188904	0.0
4	2.4341442279137923E-4	0.0	0.0	4.197560013825939	0.0
5	2.4341442279137923E-4	0.0	0.0	6.801850923270906	0.0
6	2.4341442279137923E-4	0.0	0.0	8.33943897843835	0.0
7	2.4341442279137923E-4	0.0	0.0	9.893153239115724	0.0
8	3.0426802848922406E-4	0.0	0.0	12.617751727025329	0.0
9	3.0426802848922406E-4	0.0	0.0	13.651654487831713	0.0
10	3.6512163418706884E-4	0.0	0.0	15.663474692202463	0.0
11	4.259752398849137E-4	0.0	0.0	18.33975541718798	0.0
12	4.259752398849137E-4	0.0	0.0	20.381759009984858	0.0
13	4.8682884558275846E-4	0.0	0.0	21.189164650383866	0.0
14	5.476824512806032E-4	0.0	0.0	21.501161086796714	0.0
15	5.476824512806032E-4	0.0	0.0	22.096005082493146	0.0
16	5.476824512806032E-4	0.0	0.0	23.279851127739022	0.0
17	5.476824512806032E-4	0.0	0.0	24.714657442882807	0.0
18	5.476824512806032E-4	0.0	0.0	26.370666614738255	0.0
19	5.476824512806032E-4	0.0	0.0	27.255721456007713	0.0
20	5.476824512806032E-4	0.0	0.0	28.15172994630278	0.0
21	5.476824512806032E-4	0.0	0.0	29.20601866501794	0.0
22	6.085360569784481E-4	0.0	0.0	30.274851395494885	0.0
23	6.085360569784481E-4	0.0	0.0	31.261410051068346	0.0
24	6.085360569784481E-4	0.0	0.0	32.070945567666776	0.0
25	6.085360569784481E-4	0.0	0.0	32.76972752189513	0.0
26	6.085360569784481E-4	0.0	0.0	33.41118537955611	0.0
27	6.085360569784481E-4	0.0	0.0	34.0129058326964	0.0
28	6.085360569784481E-4	0.0	0.0	34.650469059592716	0.0
29	6.085360569784481E-4	0.0	0.0	35.31395592251632	0.0
30	6.085360569784481E-4	0.0	0.0	36.05600479039584	0.0
31	6.085360569784481E-4	0.0	0.0	36.72003933577072	0.0
32	6.693896626762929E-4	0.0	0.0	37.345249280710384	0.0
33	7.302432683741377E-4	0.0	0.0	37.943135956691705	0.0
34	7.302432683741377E-4	0.0	0.0	38.52976471561893	0.0
35	7.910968740719825E-4	0.0	0.0	39.15296649157056	0.0
36	7.910968740719825E-4	0.0	0.0	39.73655257021289	0.0
37	7.910968740719825E-4	0.0	0.0	40.32336388995721	0.0
38	7.910968740719825E-4	0.0	0.0	40.905915457302676	0.0
39	7.910968740719825E-4	0.0	0.0	41.50264591477574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.034172E-4	45.000004	40
CGCGATT	25	3.8915394E-5	45.000004	30
CGTTATC	50	2.1827873E-11	45.000004	42
ACGATAG	30	2.1660726E-6	45.000004	1
CCGTACT	25	3.8915394E-5	45.000004	17
AATTGCG	20	7.034172E-4	45.000004	1
TTGCGAC	25	3.8915394E-5	45.000004	1
GCGATCG	20	7.034172E-4	45.000004	9
CGTAATA	20	7.034172E-4	45.000004	32
ACGTATG	20	7.034172E-4	45.000004	1
TCGCAAA	45	3.8562575E-10	45.0	12
ACGTTAG	45	3.8562575E-10	45.0	1
CGGTCTA	75	0.0	45.0	31
TATCTCG	65	0.0	44.999996	1
AGTCGAG	65	0.0	44.999996	1
TTAACGG	165	0.0	42.272728	2
GTTAACG	155	0.0	40.64516	1
GCGCGTT	45	1.9292202E-8	40.0	1
TGTTACG	45	1.9292202E-8	40.0	1
GCTAGCG	125	0.0	39.6	1
>>END_MODULE