FastQCFastQC Report
Sat 18 Jun 2016
SRR3550175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260962
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC79230.6283297989947357No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT72790.5772576810403486Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC56390.4471982502248283No Hit
TCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC25100.19905437277253402No Hit
ACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC22540.17875241284035523No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.1490925182519378No Hit
CGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG15790.12522185442543074No Hit
GCCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG15380.12197036865504274No Hit
ACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG15260.12101871428322186No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC14730.11681557414101296No Hit
AGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG14230.1128503475917593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG406.8157533E-945.01
ACACGAA253.8910224E-545.06
CGAAACG207.0335483E-445.032
TAAGTCG351.2120472E-745.01
CGACAAT253.8910224E-545.020
AATGCGA253.8910224E-545.025
AGACGCA253.8910224E-545.044
CGCTATA207.0335483E-445.029
CTACGAT253.8910224E-545.020
TATTACG253.8910224E-545.01
TTACGCG207.0335483E-445.01
CCACGCG207.0335483E-445.015
ATAGCCG302.1656706E-644.9999961
CCCCGCA302.1656706E-644.9999965
TAGTGCG302.1656706E-644.9999961
ATGATCG850.042.3529427
CGTGAGT850.042.3529432
TGATTCG800.042.187515
TGTCGCA800.042.187539
TTTAGCG650.041.5384641