##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550175_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1260962
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.894739096023514	33.0	31.0	34.0	30.0	34.0
2	32.03718192935235	33.0	31.0	34.0	30.0	34.0
3	32.10699688015975	33.0	31.0	34.0	30.0	34.0
4	35.69970784210785	37.0	35.0	37.0	33.0	37.0
5	35.72630023743777	37.0	35.0	37.0	35.0	37.0
6	35.73251850571231	37.0	35.0	37.0	35.0	37.0
7	35.92284462180462	37.0	35.0	37.0	35.0	37.0
8	35.87693919404391	37.0	35.0	37.0	35.0	37.0
9	37.4576378986837	39.0	37.0	39.0	35.0	39.0
10	37.21969813523326	39.0	37.0	39.0	34.0	39.0
11	37.15481275407189	39.0	37.0	39.0	34.0	39.0
12	37.00957760820707	39.0	37.0	39.0	33.0	39.0
13	36.91329397713809	39.0	37.0	39.0	33.0	39.0
14	37.96200995747691	40.0	37.0	41.0	33.0	41.0
15	38.04668261216436	40.0	37.0	41.0	33.0	41.0
16	38.099446295764665	40.0	37.0	41.0	33.0	41.0
17	38.12153022850808	40.0	37.0	41.0	33.0	41.0
18	38.03734767582211	40.0	37.0	41.0	33.0	41.0
19	38.0026083260241	40.0	37.0	41.0	33.0	41.0
20	37.879626031553684	40.0	36.0	41.0	33.0	41.0
21	37.76152175878417	39.0	36.0	41.0	33.0	41.0
22	37.74396690780531	39.0	36.0	41.0	33.0	41.0
23	37.747414275767234	39.0	36.0	41.0	33.0	41.0
24	37.81760116482495	39.0	36.0	41.0	33.0	41.0
25	37.75025258493119	39.0	36.0	41.0	33.0	41.0
26	37.687661483851215	39.0	36.0	41.0	33.0	41.0
27	37.59902677479575	39.0	36.0	41.0	33.0	41.0
28	37.51072910999697	39.0	36.0	41.0	33.0	41.0
29	37.454039059067604	39.0	36.0	41.0	33.0	41.0
30	37.35927173063106	39.0	36.0	41.0	32.0	41.0
31	37.24872676575504	39.0	35.0	41.0	32.0	41.0
32	37.11426196824329	39.0	35.0	41.0	32.0	41.0
33	37.01279578607444	39.0	35.0	41.0	31.0	41.0
34	36.84141552243446	39.0	35.0	41.0	31.0	41.0
35	36.76816906457133	39.0	35.0	41.0	31.0	41.0
36	36.670936158266464	39.0	35.0	40.0	31.0	41.0
37	36.46534312691421	39.0	35.0	40.0	30.0	41.0
38	36.369268859807036	39.0	35.0	40.0	30.0	41.0
39	36.32355296987538	39.0	35.0	40.0	30.0	41.0
40	36.09847322916948	38.0	35.0	40.0	30.0	41.0
41	36.05361303512715	38.0	35.0	40.0	30.0	41.0
42	35.9333651608851	38.0	35.0	40.0	29.0	41.0
43	35.76552425846298	38.0	35.0	40.0	29.0	41.0
44	35.63967669128808	38.0	35.0	40.0	28.0	41.0
45	35.58550614530811	38.0	34.0	40.0	28.0	41.0
46	35.582118255744426	38.0	34.0	40.0	28.0	41.0
47	35.482022455871	38.0	34.0	40.0	27.0	41.0
48	35.472071323322986	38.0	34.0	40.0	27.0	41.0
49	35.401145316036484	38.0	34.0	40.0	27.0	41.0
50	35.23348205576377	38.0	34.0	40.0	26.0	41.0
51	33.76376845614698	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	23.0
10	30.0
11	32.0
12	16.0
13	5.0
14	23.0
15	32.0
16	69.0
17	148.0
18	306.0
19	526.0
20	1007.0
21	1590.0
22	2573.0
23	3955.0
24	5931.0
25	7946.0
26	10045.0
27	12354.0
28	15344.0
29	19055.0
30	23815.0
31	30438.0
32	38993.0
33	51388.0
34	82665.0
35	107097.0
36	109554.0
37	159266.0
38	260552.0
39	316052.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.378011391302834	23.930459442869807	24.374168293731294	10.317360872096067
2	29.76909692758386	25.775320747175567	26.844504433916327	17.611077891324243
3	31.16763233150563	25.518374066783934	27.19685446508301	16.117139136627433
4	27.659120576195	27.202881609437874	26.74577029283991	18.392227521527214
5	23.915232972920673	30.797438780867303	25.72139366610572	19.5659345801063
6	26.07953292803431	34.806124213100794	24.340939695248547	14.77340316361635
7	78.32012384195559	6.763486925061976	8.945789008709223	5.9706002242732135
8	77.09518605636015	5.449331542108327	9.76437037753715	7.691112023994378
9	70.02415616013805	8.153060916982431	13.367571742843957	8.45521118003556
10	38.14595523100617	27.78576991217816	18.71777262122094	15.350502235594728
11	27.924314927809085	24.725328756933198	26.474945319525883	20.87541099573183
12	25.91687933498393	22.5550016574647	30.477603607404507	21.05051540014687
13	23.10339248922648	25.47769084238859	32.2702032257911	19.148713442593827
14	19.376872578237887	28.631473430602984	29.19651821387163	22.795135777287502
15	17.525587606922333	25.78999208540781	35.66538880632406	21.019031501345797
16	19.68766703516839	27.0725842650294	30.72812662078635	22.511622079015865
17	19.967849943138653	26.361619144748218	27.841203779336727	25.829327132776402
18	20.364213989002046	25.93749851304004	31.46906885377989	22.22921864417802
19	20.675246359525506	28.35755558058054	28.393877055771704	22.57332100412225
20	22.387589792555207	28.27547539101099	27.5374674256639	21.799467390769905
21	21.12815453598126	29.70858757044225	28.108935875942336	21.054322017634156
22	19.822167519719073	27.45871802639572	25.52392538395289	27.195189069932326
23	19.259184654256035	28.42004755099678	27.305580977063542	25.01518681768364
24	20.79769255536646	25.948204624723033	28.67747005857433	24.576632761336185
25	18.777013105866793	29.10396982621205	26.082149977556817	26.03686709036434
26	19.348243642552273	29.23886683341766	27.957464221760848	23.45542530226922
27	22.843511541188395	28.64701711867606	26.00641415046607	22.503057189669477
28	18.27073298005808	30.03405336560499	27.21644268423632	24.478770970100605
29	20.362231375727422	29.3348253159096	25.670321548151332	24.63262176021165
30	23.706106924713037	29.006583862162383	24.94595396213367	22.34135525099091
31	22.263874724218493	31.567644385794335	22.015413628642257	24.15306726134491
32	23.27595914865	32.426908979017604	21.984564166089065	22.31256770624333
33	24.702568356540482	28.776204199650746	23.575571666711607	22.94565577709717
34	20.104095127371007	29.50628171189933	25.630193455472885	24.75942970525678
35	23.387064796560086	29.133233198145543	23.724981403087483	23.754720602206884
36	25.2837119595991	29.51072276563449	25.11883783968113	20.086727435085276
37	22.86690637782899	30.15483416629526	25.435183613780588	21.543075842095163
38	22.806476325218362	29.665921732772283	25.00194296100913	22.52565898100022
39	24.242284858703115	26.196189893113353	26.544654002261765	23.016871245921763
40	24.881637987504778	23.95908837855542	28.09822976425935	23.06104386968045
41	23.780970401962946	25.227802265254624	23.9868449644002	27.004382368382235
42	26.527762137161943	24.952774151798387	25.191798008187398	23.327665702852265
43	25.868582875614017	24.801064584023944	26.982335708768385	22.348016831593657
44	22.001931858374796	26.729988691173883	25.960179608901772	25.307899841549546
45	21.666949519493848	24.85427792431493	27.011599080701878	26.467173475489346
46	24.582104773974155	25.046591411953727	27.328975813704144	23.042328000367974
47	21.49914113192943	24.229437524683537	29.58257267070697	24.688848672680063
48	22.0348432387336	23.216797968535136	28.460017034613255	26.288341758118005
49	24.28503000090407	21.942691373728945	30.19567599975257	23.576602625614413
50	21.68043128976131	21.53752452492621	30.03048466171066	26.75155952360182
51	21.33371188029457	21.142587960620542	26.62784445526511	30.89585570381978
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	348.0
1	680.5
2	1013.0
3	2142.0
4	3271.0
5	2378.5
6	1486.0
7	1572.0
8	1658.0
9	1826.5
10	1995.0
11	2174.0
12	2353.0
13	2511.0
14	2669.0
15	2876.0
16	3083.0
17	3163.0
18	3243.0
19	3450.5
20	3658.0
21	3569.0
22	3480.0
23	3728.0
24	3976.0
25	4853.0
26	7298.0
27	8866.0
28	10357.0
29	11848.0
30	14285.5
31	16723.0
32	18990.5
33	21258.0
34	23498.5
35	25739.0
36	28571.0
37	31403.0
38	35082.5
39	38762.0
40	45006.5
41	51251.0
42	60316.0
43	69381.0
44	81632.0
45	93883.0
46	115833.0
47	137783.0
48	158862.5
49	179942.0
50	172079.0
51	164216.0
52	132656.5
53	101097.0
54	83282.5
55	65468.0
56	55339.0
57	45210.0
58	41434.0
59	37658.0
60	35593.0
61	33528.0
62	30052.5
63	26577.0
64	23520.5
65	20464.0
66	16704.5
67	12945.0
68	11953.0
69	10961.0
70	8808.5
71	6656.0
72	5674.5
73	4693.0
74	3761.0
75	2330.5
76	1832.0
77	1404.5
78	977.0
79	750.5
80	524.0
81	365.5
82	207.0
83	208.0
84	209.0
85	124.0
86	39.0
87	42.0
88	45.0
89	29.0
90	13.0
91	11.0
92	9.0
93	5.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1260962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.507204220066157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.16526075887003	20.703806783661722
2	12.721285630788584	7.507391460989438
3	5.098270370794507	4.513071150004378
4	2.5909528881787436	3.058071039842417
5	1.579605043424738	2.330486430169011
6	1.0296358213985448	1.8229004672581457
7	0.7348780061915224	1.5178936783869807
8	0.5713027135389646	1.3486036671897745
9	0.42835212470800754	1.1375526259672581
>10	4.014955814709489	26.881229374715687
>50	0.9126304261299494	18.377279230235004
>100	0.14284166928462028	6.652757940628145
>500	0.006776170258386577	1.2630978582000318
>1k	0.0024394212930191673	1.219029795142461
>5k	8.131404310063892E-4	1.666828497609502
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	7923	0.6283297989947357	No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT	7279	0.5772576810403486	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	5639	0.4471982502248283	No Hit
TCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	2510	0.19905437277253402	No Hit
ACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	2254	0.17875241284035523	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1880	0.1490925182519378	No Hit
CGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG	1579	0.12522185442543074	No Hit
GCCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG	1538	0.12197036865504274	No Hit
ACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG	1526	0.12101871428322186	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC	1473	0.11681557414101296	No Hit
AGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG	1423	0.1128503475917593	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.93045309850733E-5	0.0	0.0	0.6429218326959892	0.0
2	7.93045309850733E-5	0.0	0.0	2.2584344333929174	0.0
3	7.93045309850733E-5	0.0	0.0	3.1864560549802454	0.0
4	7.93045309850733E-5	0.0	0.0	4.263411585757541	0.0
5	7.93045309850733E-5	0.0	0.0	6.932246966998212	0.0
6	7.93045309850733E-5	0.0	0.0	8.53665693335723	0.0
7	7.93045309850733E-5	0.0	0.0	10.210775582452127	0.0
8	7.93045309850733E-5	0.0	0.0	13.34933170071739	0.0
9	7.93045309850733E-5	0.0	0.0	14.689102447179216	0.0
10	7.93045309850733E-5	0.0	0.0	17.089333381973447	0.0
11	7.93045309850733E-5	0.0	0.0	20.049375000991308	0.0
12	7.93045309850733E-5	0.0	0.0	22.311457442809537	0.0
13	7.93045309850733E-5	0.0	0.0	23.246299254061583	0.0
14	7.93045309850733E-5	0.0	0.0	23.615144627673157	0.0
15	7.93045309850733E-5	0.0	0.0	24.25608384709452	0.0
16	7.93045309850733E-5	0.0	0.0	25.52162555255432	0.0
17	7.93045309850733E-5	0.0	0.0	27.062988416780204	0.0
18	7.93045309850733E-5	0.0	0.0	28.858839520937188	0.0
19	7.93045309850733E-5	0.0	0.0	29.85006685371962	0.0
20	7.93045309850733E-5	0.0	0.0	30.784987969502648	0.0
21	7.93045309850733E-5	0.0	0.0	31.88375224630084	0.0
22	1.586090619701466E-4	0.0	0.0	32.908842613813896	0.0
23	1.586090619701466E-4	0.0	0.0	33.904986827517405	0.0
24	1.586090619701466E-4	0.0	0.0	34.75838288544778	0.0
25	2.3791359295521992E-4	0.0	0.0	35.47109270541063	0.0
26	3.172181239402932E-4	0.0	0.0	36.11758324200095	0.0
27	3.172181239402932E-4	0.0	0.0	36.74083755101264	0.0
28	3.965226549253665E-4	0.0	0.0	37.3419658958795	0.0
29	3.965226549253665E-4	0.0	0.0	37.985522164823365	0.0
30	3.965226549253665E-4	0.0	0.0	38.75319002475887	0.0
31	3.965226549253665E-4	0.0	0.0	39.41086250021809	0.0
32	3.965226549253665E-4	0.0	0.0	40.030151582680524	0.0
33	3.965226549253665E-4	0.0	0.0	40.599637419684335	0.0
34	3.965226549253665E-4	0.0	0.0	41.18577720819501	0.0
35	3.965226549253665E-4	0.0	0.0	41.782940326512616	0.0
36	3.965226549253665E-4	0.0	0.0	42.375345172971116	0.0
37	4.7582718591043983E-4	0.0	0.0	42.91778816490901	0.0
38	4.7582718591043983E-4	0.0	0.0	43.4868774792579	0.0
39	4.7582718591043983E-4	0.0	0.0	44.10402533938374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	40	6.8157533E-9	45.0	1
ACACGAA	25	3.8910224E-5	45.0	6
CGAAACG	20	7.0335483E-4	45.0	32
TAAGTCG	35	1.2120472E-7	45.0	1
CGACAAT	25	3.8910224E-5	45.0	20
AATGCGA	25	3.8910224E-5	45.0	25
AGACGCA	25	3.8910224E-5	45.0	44
CGCTATA	20	7.0335483E-4	45.0	29
CTACGAT	25	3.8910224E-5	45.0	20
TATTACG	25	3.8910224E-5	45.0	1
TTACGCG	20	7.0335483E-4	45.0	1
CCACGCG	20	7.0335483E-4	45.0	15
ATAGCCG	30	2.1656706E-6	44.999996	1
CCCCGCA	30	2.1656706E-6	44.999996	5
TAGTGCG	30	2.1656706E-6	44.999996	1
ATGATCG	85	0.0	42.35294	27
CGTGAGT	85	0.0	42.35294	32
TGATTCG	80	0.0	42.1875	15
TGTCGCA	80	0.0	42.1875	39
TTTAGCG	65	0.0	41.538464	1
>>END_MODULE