FastQCFastQC Report
Sat 18 Jun 2016
SRR3550168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337584
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC69220.5175002093326475TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC67060.5013516908096987TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT47290.3535478893288197TruSeq Adapter, Index 22 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC30640.22906972571442244TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC20570.1537847342671563TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.10803059845213459No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG13860.10361966052225505TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG13560.10137681072740104No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC207.0337E-445.00000429
ATCGTAA207.0337E-445.00000445
ACCTATC207.0337E-445.00000434
CGAATTC207.0337E-445.00000416
CGTACAC207.0337E-445.00000426
TCGTACA207.0337E-445.00000434
ACATGCG406.8157533E-945.0000041
CGTAAGT207.0337E-445.00000417
CGCGTTG207.0337E-445.00000420
CGTTTAA207.0337E-445.00000410
CGAAGTT207.0337E-445.00000417
CGCCGAC453.8562575E-1045.013
TTATTCG351.21212E-745.029
ACCGGTA253.891151E-544.99999614
GCGCGTA253.891151E-544.9999961
TACGATT302.1657706E-644.99999629
TGCGATA253.891151E-544.99999613
TATAGCG600.044.9999961
ATACGAT302.1657706E-644.99999628
TGTCGAA302.1657706E-644.99999630
Produced by FastQC (version 0.11.5)