##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1337584
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03205107118506	33.0	31.0	34.0	30.0	34.0
2	32.177460256701636	34.0	31.0	34.0	30.0	34.0
3	32.23883883180421	34.0	31.0	34.0	30.0	34.0
4	35.81043059725595	37.0	35.0	37.0	35.0	37.0
5	35.83002488068039	37.0	35.0	37.0	35.0	37.0
6	35.8245291510664	37.0	35.0	37.0	35.0	37.0
7	36.01077091233149	37.0	35.0	37.0	35.0	37.0
8	35.9702755116688	37.0	35.0	37.0	35.0	37.0
9	37.53736812043206	39.0	37.0	39.0	35.0	39.0
10	37.29193755308078	39.0	37.0	39.0	34.0	39.0
11	37.23432023708418	39.0	37.0	39.0	34.0	39.0
12	37.07191847390519	39.0	37.0	39.0	33.0	39.0
13	36.98761797389921	39.0	37.0	39.0	33.0	39.0
14	38.0752700391153	40.0	37.0	41.0	33.0	41.0
15	38.14356107728561	40.0	37.0	41.0	33.0	41.0
16	38.20357226162992	40.0	37.0	41.0	34.0	41.0
17	38.20672944652448	40.0	37.0	41.0	34.0	41.0
18	38.10820853120253	40.0	37.0	41.0	33.0	41.0
19	38.05570865082118	40.0	37.0	41.0	34.0	41.0
20	37.94322450029306	40.0	36.0	41.0	33.0	41.0
21	37.8506366702951	40.0	36.0	41.0	33.0	41.0
22	37.82369780140911	40.0	36.0	41.0	33.0	41.0
23	37.81954329597244	40.0	36.0	41.0	33.0	41.0
24	37.88812440938289	40.0	36.0	41.0	33.0	41.0
25	37.82773941673943	40.0	36.0	41.0	33.0	41.0
26	37.756103541908395	40.0	36.0	41.0	33.0	41.0
27	37.659438958599985	40.0	36.0	41.0	33.0	41.0
28	37.56676739554301	39.0	36.0	41.0	33.0	41.0
29	37.502178554767404	39.0	36.0	41.0	33.0	41.0
30	37.36797763729231	39.0	36.0	41.0	32.0	41.0
31	37.226984623021806	39.0	35.0	41.0	32.0	41.0
32	37.158524623500284	39.0	35.0	41.0	32.0	41.0
33	37.087684960346415	39.0	35.0	41.0	32.0	41.0
34	36.913533654708786	39.0	35.0	41.0	31.0	41.0
35	36.88586511202287	39.0	35.0	41.0	31.0	41.0
36	36.7911876936327	39.0	35.0	41.0	31.0	41.0
37	36.7134751910908	39.0	35.0	40.0	31.0	41.0
38	36.66673644421584	39.0	35.0	40.0	31.0	41.0
39	36.622148590294145	39.0	35.0	40.0	31.0	41.0
40	36.44977885501023	39.0	35.0	40.0	30.0	41.0
41	36.35542066890752	38.0	35.0	40.0	30.0	41.0
42	36.315239267216114	38.0	35.0	40.0	30.0	41.0
43	36.288589725953656	38.0	35.0	40.0	30.0	41.0
44	36.186406984533306	38.0	35.0	40.0	30.0	41.0
45	36.06160958863144	38.0	35.0	40.0	30.0	41.0
46	35.99791340207419	38.0	35.0	40.0	30.0	41.0
47	35.909536896374355	38.0	35.0	40.0	30.0	41.0
48	35.94061980406464	38.0	35.0	40.0	30.0	41.0
49	35.894675026017055	38.0	35.0	40.0	29.0	41.0
50	35.78623174320267	38.0	35.0	40.0	29.0	41.0
51	34.44613571932678	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	33.0
10	55.0
11	44.0
12	30.0
13	31.0
14	32.0
15	41.0
16	73.0
17	169.0
18	266.0
19	442.0
20	856.0
21	1416.0
22	2201.0
23	3274.0
24	5006.0
25	6859.0
26	9204.0
27	11435.0
28	14243.0
29	17766.0
30	22695.0
31	29477.0
32	38352.0
33	52160.0
34	88579.0
35	111163.0
36	114920.0
37	168104.0
38	281170.0
39	357383.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.032189380255744	21.90696060957667	25.87568332157083	11.185166688596754
2	30.995511309943897	25.03954891804926	25.034315601861266	18.930624170145578
3	32.59488749865429	23.32212406846972	26.59339525592411	17.489593176951878
4	28.69868359669374	26.15701144750535	25.62702604098135	19.517278914819556
5	26.222427899855262	30.12401464132346	23.927394466440983	19.726162992380292
6	28.13124259859568	32.967649134559025	23.1637041112932	15.7374041555521
7	78.83071268795081	6.560709458247108	9.154116676036795	5.4544611777652845
8	78.1806600557423	5.39958611945119	9.531214488211582	6.888539336594936
9	71.1359436117657	8.089211593440112	12.426210241749303	8.348634553044892
10	38.57305410351798	25.651772150384573	19.532006961805763	16.24316678429168
11	30.69788514216677	24.454763214870994	23.70101616048039	21.14633548248185
12	27.29249153698011	21.304605916338712	30.131715092285795	21.27118745439539
13	24.646825918970322	23.536465747197933	31.072889627866363	20.743818705965385
14	20.10572793932942	27.804160336846135	29.17588727137884	22.914224452445602
15	19.018842928743165	25.14683189990311	34.85366152705176	20.980663644301963
16	21.214518116245408	24.97996387516597	30.65863527075683	23.146882737831792
17	21.398431799423438	24.587689446046003	28.066648524503883	25.947230230026676
18	22.269554659744735	24.87611992966423	30.029889711599424	22.824435698991614
19	23.258427134295864	26.319991865931406	27.122857330829316	23.298723668943406
20	25.456195648273304	25.44976614552806	27.547279273675525	21.546758932523115
21	24.156090383856267	26.396024473976958	27.971701216521726	21.476183925645042
22	21.47924915369801	25.614839890429312	26.331430399885164	26.574480555987513
23	22.785933444179953	25.647211689135034	27.253540712209478	24.31331415447553
24	24.110261486381415	22.97822043325877	28.576149236234883	24.33536884412493
25	21.831600856469574	26.125237742078255	25.951192597997586	26.091968803454584
26	21.080171413533655	28.204882833526717	26.454861900261967	24.260083852677663
27	22.72948839100946	28.39627268268759	26.221680283256976	22.65255864304597
28	19.75360052153734	29.0050568786708	26.922421320829198	24.318921278962666
29	23.18702974915968	27.639908970202992	25.648034067393148	23.525027213244176
30	24.767491237933466	27.956076029617577	24.337013600641157	22.9394191318078
31	23.194954485101498	29.506109522841186	23.965896721252648	23.333039270804676
32	24.58791373102549	29.452505412744173	22.48479347839089	23.474787377839448
33	24.687271976937524	27.737921506238113	23.472170719745453	24.10263579707891
34	22.223576238950226	27.126969222119886	27.282922044522063	23.366532494407828
35	23.130435172669532	26.092043565114416	24.76741647627364	26.010104785942417
36	22.117414681993804	30.02263783059606	24.01688417325566	23.843063314154474
37	22.999078936350912	27.910994748741015	26.801157908587424	22.28876840632065
38	23.239512434359263	27.283370614481033	23.81308388856326	25.66403306259644
39	25.423225756288954	23.01096604026364	26.43303149559205	25.13277670785536
40	24.405943851003002	22.950782904101725	29.371987105108914	23.271286139786362
41	19.235726503905546	26.228483594301366	26.03619660522255	28.499593296570534
42	21.08772234117633	25.6242598595677	26.42293867151521	26.86507912774076
43	23.816747209894856	24.755080802401945	26.421966769937438	25.006205217765764
44	23.34986064426608	24.831636742066294	25.731094271462577	26.08740834220505
45	23.726435124822068	22.989285158913383	26.29950717113841	26.98477254512614
46	23.644645868969725	24.764650294859987	26.736489072835802	24.85421476333449
47	20.42017548056795	25.009569492458045	29.8396960489958	24.730558977978205
48	21.045482003373245	22.55320039713394	28.33085623033768	28.07046136915513
49	22.406518020550482	22.53540712209476	29.70908742927547	25.348987428079283
50	21.95181760547375	22.034130192944893	28.73972774793957	27.27432445364179
51	20.61762102417493	21.808574265242406	26.844968241246903	30.728836469335757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	382.0
1	1196.0
2	2010.0
3	2673.5
4	3337.0
5	2494.5
6	1652.0
7	1632.0
8	1612.0
9	1645.0
10	1678.0
11	1774.5
12	1871.0
13	1971.5
14	2072.0
15	2284.5
16	2497.0
17	2396.0
18	2295.0
19	2547.0
20	2799.0
21	3209.0
22	3619.0
23	4228.0
24	4837.0
25	4962.5
26	7309.0
27	9530.0
28	10690.0
29	11850.0
30	14719.5
31	17589.0
32	20230.5
33	22872.0
34	25590.5
35	28309.0
36	31349.0
37	34389.0
38	36315.0
39	38241.0
40	43613.0
41	48985.0
42	56978.0
43	64971.0
44	75580.0
45	86189.0
46	103805.5
47	121422.0
48	138150.5
49	154879.0
50	151834.5
51	148790.0
52	125526.5
53	102263.0
54	88883.5
55	75504.0
56	69649.0
57	63794.0
58	61616.0
59	59438.0
60	58544.5
61	57651.0
62	52167.5
63	46684.0
64	40083.5
65	33483.0
66	28702.5
67	23922.0
68	20721.5
69	17521.0
70	15116.5
71	12712.0
72	10997.0
73	9282.0
74	7446.5
75	4283.0
76	2955.0
77	2318.5
78	1682.0
79	1211.0
80	740.0
81	535.0
82	330.0
83	246.0
84	162.0
85	108.5
86	55.0
87	36.5
88	18.0
89	12.0
90	6.0
91	5.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1337584.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.23649308467438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.15921923035428	15.605447332182578
2	12.67252540911556	5.889300980685482
3	5.6106682553911	3.9111676235039208
4	3.0350993343534673	2.8210025877601663
5	1.9235667062949473	2.2348472234366206
6	1.2992613053912283	1.8114165802744966
7	0.9036209657542337	1.4697887625340527
8	0.6750014793466746	1.2547733765587183
9	0.5334198611495861	1.1155326223347293
>10	4.30302562952552	22.584134668770666
>50	1.4391552235689222	24.15743644670283
>100	0.4344061231404389	13.629564178825845
>500	0.007461793003652817	1.1720886943336764
>1k	0.0029198320449076243	1.3161497629516117
>5k	6.488515655350276E-4	1.027349159144542
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	6922	0.5175002093326475	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	6706	0.5013516908096987	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	4729	0.3535478893288197	TruSeq Adapter, Index 22 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	3064	0.22906972571442244	TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	2057	0.1537847342671563	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1445	0.10803059845213459	No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	1386	0.10361966052225505	TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	1356	0.10137681072740104	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.38083589516621014	0.0
2	0.0	0.0	0.0	1.9094127920190433	0.0
3	0.0	0.0	0.0	2.6171066639553104	0.0
4	0.0	0.0	0.0	3.4598200935417887	0.0
5	0.0	0.0	0.0	5.7179212670008015	0.0
6	0.0	0.0	0.0	6.885548870201797	0.0
7	0.0	0.0	0.0	8.153132812593451	0.0
8	0.0	0.0	0.0	10.079666024713214	0.0
9	0.0	0.0	0.0	10.842234954963576	0.0
10	0.0	0.0	0.0	12.419556454024569	0.0
11	0.0	0.0	0.0	14.604989294130313	0.0
12	0.0	0.0	0.0	16.26267957750691	0.0
13	0.0	0.0	0.0	16.942487350327156	0.0
14	0.0	0.0	0.0	17.230618787306067	0.0
15	0.0	0.0	0.0	17.781238411942727	0.0
16	0.0	0.0	0.0	18.87036627232383	0.0
17	0.0	0.0	0.0	20.14998684194787	0.0
18	0.0	0.0	0.0	21.535170875249705	0.0
19	0.0	0.0	0.0	22.318598308592208	7.476165982846685E-5
20	0.0	0.0	0.0	23.049692580054785	7.476165982846685E-5
21	0.0	0.0	0.0	23.980624768238854	7.476165982846685E-5
22	0.0	0.0	0.0	24.896455101137573	7.476165982846685E-5
23	0.0	0.0	0.0	25.77909125707245	7.476165982846685E-5
24	0.0	0.0	0.0	26.495831289847963	7.476165982846685E-5
25	0.0	0.0	0.0	27.130782066771133	7.476165982846685E-5
26	0.0	0.0	0.0	27.695456883455545	7.476165982846685E-5
27	0.0	0.0	0.0	28.268355482721084	7.476165982846685E-5
28	0.0	0.0	0.0	28.82361033026711	7.476165982846685E-5
29	0.0	0.0	0.0	29.38432277898061	7.476165982846685E-5
30	0.0	0.0	0.0	30.025404012009712	7.476165982846685E-5
31	0.0	0.0	0.0	30.625366332133158	7.476165982846685E-5
32	0.0	0.0	0.0	31.211572506848167	7.476165982846685E-5
33	0.0	0.0	0.0	31.78013492984366	7.476165982846685E-5
34	0.0	0.0	0.0	32.34077261689733	7.476165982846685E-5
35	0.0	0.0	0.0	32.89557889448438	7.476165982846685E-5
36	0.0	0.0	0.0	33.47251462338066	7.476165982846685E-5
37	0.0	0.0	0.0	34.020368066603666	7.476165982846685E-5
38	0.0	0.0	0.0	34.577865763944544	7.476165982846685E-5
39	0.0	0.0	0.0	35.13013014509743	7.476165982846685E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	20	7.0337E-4	45.000004	29
ATCGTAA	20	7.0337E-4	45.000004	45
ACCTATC	20	7.0337E-4	45.000004	34
CGAATTC	20	7.0337E-4	45.000004	16
CGTACAC	20	7.0337E-4	45.000004	26
TCGTACA	20	7.0337E-4	45.000004	34
ACATGCG	40	6.8157533E-9	45.000004	1
CGTAAGT	20	7.0337E-4	45.000004	17
CGCGTTG	20	7.0337E-4	45.000004	20
CGTTTAA	20	7.0337E-4	45.000004	10
CGAAGTT	20	7.0337E-4	45.000004	17
CGCCGAC	45	3.8562575E-10	45.0	13
TTATTCG	35	1.21212E-7	45.0	29
ACCGGTA	25	3.891151E-5	44.999996	14
GCGCGTA	25	3.891151E-5	44.999996	1
TACGATT	30	2.1657706E-6	44.999996	29
TGCGATA	25	3.891151E-5	44.999996	13
TATAGCG	60	0.0	44.999996	1
ATACGAT	30	2.1657706E-6	44.999996	28
TGTCGAA	30	2.1657706E-6	44.999996	30
>>END_MODULE