##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550167_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1859694 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04642968144221 33.0 31.0 34.0 30.0 34.0 2 32.200126472419655 34.0 31.0 34.0 30.0 34.0 3 32.249127544639066 34.0 31.0 34.0 30.0 34.0 4 35.81862553731958 37.0 35.0 37.0 35.0 37.0 5 35.82464426943358 37.0 35.0 37.0 35.0 37.0 6 35.81988703517891 37.0 35.0 37.0 35.0 37.0 7 36.00465990641471 37.0 35.0 37.0 35.0 37.0 8 35.95609116338495 37.0 35.0 37.0 35.0 37.0 9 37.512731126733755 39.0 37.0 39.0 35.0 39.0 10 37.2523103263225 39.0 37.0 39.0 34.0 39.0 11 37.18265962034614 39.0 37.0 39.0 34.0 39.0 12 37.03566877131399 39.0 37.0 39.0 33.0 39.0 13 36.97044728863996 39.0 37.0 39.0 33.0 39.0 14 38.07697072744226 40.0 37.0 41.0 33.0 41.0 15 38.161465811042035 40.0 37.0 41.0 33.0 41.0 16 38.21176817261335 40.0 37.0 41.0 33.0 41.0 17 38.2117256925064 40.0 37.0 41.0 34.0 41.0 18 38.106577211089565 40.0 37.0 41.0 33.0 41.0 19 38.072478590563826 40.0 37.0 41.0 34.0 41.0 20 37.95245992082568 40.0 37.0 41.0 33.0 41.0 21 37.84996563950843 40.0 36.0 41.0 33.0 41.0 22 37.830277991970725 40.0 36.0 41.0 33.0 41.0 23 37.82003813530613 40.0 36.0 41.0 33.0 41.0 24 37.85323553229725 40.0 36.0 41.0 33.0 41.0 25 37.791171020608765 40.0 36.0 41.0 33.0 41.0 26 37.73254470896825 40.0 36.0 41.0 33.0 41.0 27 37.64071508538502 40.0 36.0 41.0 33.0 41.0 28 37.53436264245623 40.0 36.0 41.0 33.0 41.0 29 37.474376429670684 40.0 36.0 41.0 32.0 41.0 30 37.377758921629045 39.0 36.0 41.0 32.0 41.0 31 37.29131351717003 39.0 35.0 41.0 32.0 41.0 32 37.21560751392433 39.0 35.0 41.0 32.0 41.0 33 37.146367628222706 39.0 35.0 41.0 32.0 41.0 34 37.03324471660392 39.0 35.0 41.0 31.0 41.0 35 36.97759093700361 39.0 35.0 41.0 31.0 41.0 36 36.88414169212785 39.0 35.0 41.0 31.0 41.0 37 36.81654454980228 39.0 35.0 41.0 31.0 41.0 38 36.689343515653654 39.0 35.0 41.0 31.0 41.0 39 36.66978008209953 39.0 35.0 41.0 31.0 41.0 40 36.54384215897884 39.0 35.0 41.0 30.0 41.0 41 36.426949810022506 39.0 35.0 40.0 30.0 41.0 42 36.3149061082092 39.0 35.0 40.0 30.0 41.0 43 36.21630063870723 38.0 35.0 40.0 30.0 41.0 44 36.11292449187877 38.0 35.0 40.0 30.0 41.0 45 36.0105468964249 38.0 35.0 40.0 29.0 41.0 46 35.94159953196601 38.0 35.0 40.0 29.0 41.0 47 35.8237559512479 38.0 35.0 40.0 29.0 41.0 48 35.79528029880185 38.0 35.0 40.0 29.0 41.0 49 35.710026488228706 38.0 35.0 40.0 29.0 41.0 50 35.547797110707464 37.0 34.0 40.0 29.0 41.0 51 34.1471376473764 36.0 33.0 39.0 25.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 5.0 8 32.0 9 70.0 10 85.0 11 65.0 12 40.0 13 30.0 14 48.0 15 73.0 16 134.0 17 241.0 18 434.0 19 815.0 20 1332.0 21 2291.0 22 3606.0 23 5321.0 24 7646.0 25 10568.0 26 13887.0 27 17332.0 28 21196.0 29 26493.0 30 32678.0 31 40989.0 32 52328.0 33 71544.0 34 119889.0 35 147024.0 36 155331.0 37 223557.0 38 373703.0 39 530730.0 40 176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1564579979287 20.728356385512885 24.383258751170892 10.731926865387532 2 31.548899980319344 23.979267556920654 25.366968974465692 19.10486348829431 3 31.858735899561967 24.442246950304728 26.532483301016192 17.166533849117112 4 29.21206392019332 26.37331733070064 25.126983256385188 19.287635492720845 5 25.767626286905266 30.74349866160777 23.622488430892393 19.86638662059457 6 28.632667524872375 34.29300734421899 23.007225919963176 14.067099210945457 7 80.55776918138146 6.602107658571786 7.90194515871966 4.9381780013271 8 79.69655222848489 6.1152533696403815 8.431064465444315 5.757129936430402 9 72.65550138893818 8.352180520021035 11.145166893047996 7.847151197992789 10 39.52865363871691 25.99750281497924 18.23402129597665 16.239822250327205 11 30.556855052497884 25.255982973542963 23.63862011707302 20.548541856886132 12 27.65944289759498 22.15853791000025 29.26879368326187 20.9132255091429 13 25.280879542548394 23.6123254685986 30.316923106704653 20.789871882148354 14 21.46353109705145 27.595776509468763 28.141403908384927 22.79928848509486 15 20.67673498973487 25.999277300459106 32.551753137881825 20.772234571924198 16 22.616355163806517 25.232161850282896 30.700964782378175 21.45051820353241 17 22.55435571658563 25.114669402600644 27.636482130931217 24.694492749882507 18 23.10159628411986 25.607707504567955 29.224539090839674 22.066157120472507 19 24.588722660824846 26.7540251245635 26.23603668130348 22.421215533308168 20 26.739883013011816 25.94125700249611 26.277602659362238 21.041257325129834 21 25.03723730893362 25.84323012280515 27.185762819044424 21.933769749216804 22 23.14827062946915 24.603348722961947 26.19866494165169 26.049715705917208 23 23.05675019653771 25.871675662770325 27.015358440689706 24.05621570000226 24 24.298621171009856 23.951736145839046 27.730798722800632 24.018843960350466 25 23.594526841512636 25.832045487053247 25.594909700197988 24.978517971236126 26 22.569143095584543 26.637500578052087 26.881089039379596 23.912267286983774 27 25.20194182483785 26.403698673007497 25.78445701282039 22.609902489334267 28 22.262479741290772 26.719503316136954 26.77026435531867 24.2477525872536 29 24.309590717612682 25.322176659170808 25.830109684711573 24.538122938504937 30 25.75730200774966 24.975506723149078 24.908613997786734 24.358577271314527 31 24.514140498383068 26.7300964567289 24.295448606060997 24.460314438827034 32 24.925928674287277 26.763811680846416 24.553340495801997 23.75691914906431 33 24.4185333716192 26.816831156093425 24.37105244196088 24.393583030326493 34 22.10605615762593 26.443328848724573 27.161726606635284 24.288888387014207 35 24.28657617866165 24.468057648193735 26.234154651249074 25.01121152189554 36 23.89285549127975 28.672566562025796 24.597917721947805 22.83666022474665 37 23.547261001003392 27.451021512141242 27.136883809917116 21.86483367693825 38 21.095943741282166 27.369825358365407 26.390578234913914 25.14365266543851 39 22.676203719536655 25.388908067671352 26.66444049397374 25.270447718818257 40 23.738098848520238 23.352605321090458 30.148669619840685 22.76062621054862 41 21.202305325499786 25.725845219697437 28.423009376811454 24.648840077991323 42 23.1538629473451 26.109402944785543 27.597120816650484 23.13961329121888 43 24.39326039660288 25.074609048585412 27.02240261032191 23.509727944489793 44 22.773746648642195 25.39579092044175 25.778165655209946 26.052296775706115 45 23.104446215345106 23.520159768219933 26.634596874539575 26.74079714189539 46 23.468215738718307 24.538230483079474 26.85527834149059 25.13827543671163 47 21.274736596450815 25.452036732924878 29.603526171509937 23.66970049911437 48 21.863973320341948 24.174837365717156 28.43489305229785 25.526296261643044 49 22.001737920324526 24.33335806858548 28.475222267749427 25.189681743340568 50 20.581880674992767 23.20489284796316 29.65224386377544 26.560982613268635 51 21.053463634339842 22.963616594988206 27.01030384568644 28.972615924985508 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 483.0 1 1551.5 2 2620.0 3 3754.5 4 4889.0 5 3607.5 6 2326.0 7 2312.0 8 2298.0 9 2300.0 10 2302.0 11 2509.0 12 2716.0 13 2764.0 14 2812.0 15 3069.5 16 3327.0 17 3385.5 18 3444.0 19 3845.0 20 4246.0 21 4921.5 22 5597.0 23 5822.5 24 6048.0 25 8408.0 26 12772.0 27 14776.0 28 16678.0 29 18580.0 30 22947.5 31 27315.0 32 29796.5 33 32278.0 34 37172.5 35 42067.0 36 47335.5 37 52604.0 38 56159.0 39 59714.0 40 65664.5 41 71615.0 42 82208.0 43 92801.0 44 104542.0 45 116283.0 46 132611.5 47 148940.0 48 168115.0 49 187290.0 50 184905.5 51 182521.0 52 159834.5 53 137148.0 54 123660.5 55 110173.0 56 103334.0 57 96495.0 58 91592.0 59 86689.0 60 85008.0 61 83327.0 62 77629.5 63 71932.0 64 61342.5 65 50753.0 66 43071.5 67 35390.0 68 31570.5 69 27751.0 70 24112.0 71 20473.0 72 18234.5 73 15996.0 74 13004.5 75 8153.0 76 6293.0 77 4692.0 78 3091.0 79 2371.0 80 1651.0 81 1254.5 82 858.0 83 770.5 84 683.0 85 430.0 86 177.0 87 138.0 88 99.0 89 62.0 90 25.0 91 17.5 92 10.0 93 7.0 94 4.0 95 3.5 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1859694.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.34859594234214 #Duplication Level Percentage of deduplicated Percentage of total 1 68.35108045941676 14.59199598950606 2 12.416297686196371 5.301410448048878 3 5.330945853657012 3.414246270606832 4 2.8931935414056795 2.470624795938551 5 1.7085423339904542 1.8237489969374188 6 1.1159309828993027 1.4294135792074756 7 0.8111057287359733 1.2121177928512286 8 0.586233314685558 1.0012206530529508 9 0.4334446953101958 0.8328092097176073 >10 4.007891632484996 19.58404308194977 >50 1.6097541226621739 25.02125673314417 >100 0.7251223792071246 20.598278294445443 >500 0.006886494449004942 0.9451014263654847 >1k 0.003060664199557752 1.098979206564186 >5k 5.10110699926292E-4 0.6747535216639445 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC 6552 0.352316026184953 TruSeq Adapter, Index 23 (95% over 22bp) CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC 5840 0.3140301576495918 TruSeq Adapter, Index 23 (95% over 22bp) CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT 4072 0.218960753758414 TruSeq Adapter, Index 20 (95% over 24bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2206 0.11862166571489718 No Hit TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC 2175 0.11695472480956544 TruSeq Adapter, Index 23 (95% over 22bp) GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 2006 0.10786720826114404 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.1508914907506288E-4 0.0 0.0 0.24229792643305834 0.0 2 2.1508914907506288E-4 0.0 0.0 1.2038001950858581 0.0 3 2.1508914907506288E-4 0.0 0.0 1.6839329481086673 0.0 4 2.1508914907506288E-4 0.0 0.0 2.2567153520955596 0.0 5 2.1508914907506288E-4 0.0 0.0 3.8262208728962936 0.0 6 2.1508914907506288E-4 0.0 0.0 4.680554973022444 0.0 7 2.1508914907506288E-4 0.0 0.0 5.568550524978841 0.0 8 2.1508914907506288E-4 0.0 0.0 7.086112016277947 0.0 9 2.1508914907506288E-4 0.0 0.0 7.681263691768645 0.0 10 2.1508914907506288E-4 0.0 0.0 8.917757437513913 0.0 11 2.1508914907506288E-4 0.0 0.0 10.51597735971617 0.0 12 2.1508914907506288E-4 0.0 0.0 11.681437913979396 0.0 13 2.1508914907506288E-4 0.0 0.0 12.189048305796545 0.0 14 2.1508914907506288E-4 0.0 0.0 12.394512215450499 0.0 15 2.1508914907506288E-4 0.0 0.0 12.805117401034794 0.0 16 2.1508914907506288E-4 0.0 0.0 13.63390966470828 0.0 17 2.1508914907506288E-4 0.0 0.0 14.574118107602649 0.0 18 2.688614363438286E-4 0.0 0.0 15.645584703720074 0.0 19 2.688614363438286E-4 0.0 0.0 16.20949468030762 0.0 20 2.688614363438286E-4 0.0 0.0 16.787009045574166 0.0 21 2.688614363438286E-4 0.0 0.0 17.482284719959306 0.0 22 3.2263372361259434E-4 0.0 0.0 18.212082202770993 0.0 23 3.2263372361259434E-4 0.0 0.0 18.90805691689063 0.0 24 3.2263372361259434E-4 0.0 0.0 19.48444206412453 0.0 25 3.764060108813601E-4 0.0 0.0 19.990439287323614 0.0 26 3.764060108813601E-4 0.0 0.0 20.44637451107548 0.0 27 3.764060108813601E-4 0.0 0.0 20.89198545567174 0.0 28 3.764060108813601E-4 0.0 0.0 21.379216150614027 0.0 29 3.764060108813601E-4 0.0 0.0 21.868920370770674 0.0 30 3.764060108813601E-4 0.0 0.0 22.427399346344075 0.0 31 3.764060108813601E-4 0.0 0.0 22.93479464901215 0.0 32 3.764060108813601E-4 0.0 0.0 23.446760596098066 0.0 33 3.764060108813601E-4 0.0 0.0 23.92103216980858 0.0 34 3.764060108813601E-4 0.0 0.0 24.42299647146251 0.0 35 3.764060108813601E-4 0.0 0.0 24.91915336609141 0.0 36 4.3017829815012576E-4 0.0 0.0 25.41681588476384 0.0 37 4.839505854188915E-4 0.0 0.0 25.93034122818055 0.0 38 6.452674472251887E-4 0.0 0.0 26.40945230774525 0.0 39 6.452674472251887E-4 0.0 0.0 26.906792192694066 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAAACG 30 2.166229E-6 45.000004 14 TAAACCG 30 2.166229E-6 45.000004 36 GGATCGA 30 2.166229E-6 45.000004 8 TTCGTCG 30 2.166229E-6 45.000004 1 TCGAAAC 30 2.166229E-6 45.000004 13 GTCGATA 20 7.034414E-4 45.0 39 CGTATCA 40 6.8193913E-9 45.0 25 CCGCTTA 20 7.034414E-4 45.0 13 CCGTATC 25 3.891739E-5 45.0 4 TCGTCAA 20 7.034414E-4 45.0 14 GTCGTAC 20 7.034414E-4 45.0 22 CAATACG 25 3.891739E-5 45.0 30 TACGTCG 25 3.891739E-5 45.0 13 TCTATCG 20 7.034414E-4 45.0 43 CGAATAT 295 0.0 42.711864 14 TACGAAT 315 0.0 41.428574 12 CGTACAT 60 3.6379788E-12 41.250004 35 CTAACGG 115 0.0 41.086956 2 ATTGCGT 55 6.184564E-11 40.909092 12 TTAACGG 50 1.0822987E-9 40.5 2 >>END_MODULE